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Citations for Agilent :
8401 - 8450 of 8609 citations for Cyclic GMP cGMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed a further 3 times in TBS-T for 5 minutes and developed with DAB diluted at a 1:50 ratio in DAB-chromagen (Agilent; #K3468) until appearance of brown staining ...
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bioRxiv - Molecular Biology 2023Quote: ... desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies) coupled to a 5975B mass spectrometer (Agilent Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... 500 mg (1 mL) of sample powder was transferred immediately into a 20-mL headspace vial (Agilent, Palo Alto, CA, USA) that contained an NaCl-saturated solution ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and incubated with HRP-conjugated secondary antibodies for 1 h at RT (Goat-anti-Rabbit-HRP, Goat-anti-Mouse-HRP, both Dako Denmark). Marker lanes were incubated with Streptavidin-biotinylated-HRP (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
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bioRxiv - Microbiology 2023Quote: ... approximately 2 µg of protein was injected on a Zorbax Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) and separated using a 15 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Plant Biology 2023Quote: ... the pET-28a plasmid bearing WT CG-1 domain or its six-point mutants (M1 to M6) were transformed into BL21-Codon Plus (DE3)-RIL (Stratagene, USA) cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed in a Tris-EDTA buffer for 30 min at 95°C for Ki-67 (1:200, Dako, M7240), ATRX (1:400 ...
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bioRxiv - Plant Biology 2023Quote: ... allowing a sub-sample (∼1 μg) into the pyrolizer of the GC/MS apparatus (Agilent, 7890A/5975C, Agilent Technologies AB, Sweden). Following pyrolysis ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: The tissue sections used for the Visium spatial experiments were stained with DAPI Fluoromount G and Ki67-Cy5 (1:100 DAKO #M7420). The individual channels corresponding to DAPI and Cy5 were scanned and saved as TIFF files ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-SDPN-1 SH3 (P479L) fusion proteins were expressed in the ArcticExpress strain of Escherichia coli (Stratagene, La Jolla, CA). Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 was scored on the glass slide of whole tissue sections by a breast pathologist (BC) according to the Ki-67 IHC MIB-1 pharmDx (Dako Omnis) Interpretation Manual for Breast Carcinoma ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were the following: goat anti-mouse IgG conjugated to horseradish peroxidase (IB: 1:10,000, cat#P044701-2 Dako, Glostrup, Denmark), donkey anti-Rabbit IgG Alexa Fluor 488 (IF ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... neurons were incubated for 1 hour in CO2-free incubator in Agilent Seahorse DMEM assay medium (Agilent Technologies, cat# 103575-100) supplemented with 1 mM sodium pyruvate (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... 11 cycles of TCR target enrichment PCR 1 and 2 were performed and the resulting cDNA was quantified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). TCR libraries were prepared and indexed with 9 cycles of amplification using the PN-220103 Chromium i7 Sample Index Plate ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with primary antibodies (chicken anti-Map2 [Thermo Scientific, PA1-16751, 1:1000] and rabbit anti-Gfap [Dako, Z0334 ...
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bioRxiv - Biochemistry 2023Quote: ... blots were incubated for 1h at room temperature with a horse radish peroxidase-labeled secondary antibody (anti-rabbit, Dako; 1/1000), and revealed by ECL chemiluminescence (Millipore ...
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bioRxiv - Pathology 2023Quote: ... EGTA 1 mM and 1mg/ml bovine serum albumin) supplemented with respiratory substrates (glutamate/malate) and 50 nM MitoXpress (Agilent, USA). Each well was loaded with 10μl respiratory substrates ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with DAPI (1 μg/ml) and mounted onto glass slides using DAKO Fluorescent Mounting Medium (Agilent Technologies S3023). Images were acquired using an Upright ZEISS AXIO Imager.M2 with Apotome 2.
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bioRxiv - Plant Biology 2024Quote: ... Powdered samples (1 g) were weighed and transferred immediately to a 20 mL head-space vial (Agilent, Palo Alto, CA, USA), containing NaCl saturated solution to inhibit potential enzyme reactions ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with Alexa Fluor®-conjugated secondary antibodies for 1 hour at room temperature and mounted on slides using DAPI-containing mounting media (Dako). Images were acquired using a Zeiss LSM 800 series confocal microscope at the Yale CCMI facility ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody against pS65-Ub (in- house, 1:250) [20] that was diluted in Dako Antibody Diluent with Background Reducing Components (Agilent, S3022) for 45 minutes at room temperature ...
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bioRxiv - Pathology 2024Quote: ... Van-Gieson as a specific elastin stain or labelled with alpha-smooth muscle actin (a-SMA, Cat#M0851, Dako, 1:1000) as a specific marker for muscle ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μL of derivatized sample was injected into a J&W HP-5ms Ultra Inert GC column (Agilent Technologies, #19091S-433UI) using a 1:10 split mode ...
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bioRxiv - Neuroscience 2024Quote: ... Then membranes were probed with the following antibodies: anti-human tau polyclonal antiserum KJ9A (1:5,000; A0024, Dako Cooperation, Carpinteria, CA) and rabbit anti-GAPDH antiserum (1:5,000 ...
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bioRxiv - Plant Biology 2024Quote: ... Fatty acid methyl esters (1 ml) were injected onto a DB-23 capillary column (60 m · 0.25 mm · 0.25 mm (Agilent Technologies, United States). A mix of FAME standards was used as references for peak identification by retention times ...
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bioRxiv - Plant Biology 2019Quote: ... RNA quality and integrity were evaluated by electrophoresis on 1% agarose gel and the RNA integrity number (RIN) was analysed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.).
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bioRxiv - Microbiology 2019Quote: ... We extracted extracellular metabolites using solid phase extraction (SPE) with 1 g/6 cc PPL cartridges (BondElut, Agilent, Santa Clara, CA, USA) as described previously (Dittmar et al ...
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bioRxiv - Immunology 2021Quote: ... and bound serum IgG was detected by 70 µL/well of 1:3000 diluted rabbit anti-human IgG antibody linked to horseradish peroxidase (Agilent, P021402-2) incubated for 2h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was diluted (1:10-20) and used for qRT-PCR in technical triplicate using Brilliant III SYBR master mix (Agilent Technologies, 600882) and CFX384 Touch Real-Time PCR Detection Systems (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako, Cambridge, UK). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated for 2 hours at room temperature with a biotinylated goat anti-rabbit secondary antibody (Dako, 1:500 in blocking solution). Cells were washed 3 times and incubated for 30 minutes with streptavidin Cy3 (Sigma ...
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bioRxiv - Physiology 2019Quote: ... At least 10 sections per pancreas spaced at least 100 μm apart were stained with the following antibodies: Guinea pig anti-insulin (Dako, 1:500), Rabbit anti-proglucagon (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Five mL of headspace gas sample from the 20 mL culture bottle was administered via a 1 mL GSV Loop to the GC-MS (Agilent 7890A/5975C). The volatile compounds were separated by CP7348 column (Agilent PoraBOND Q 25 m × 250 µm × 3 µm ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μl of each PCR product was subjected to Chip-based capillary electrophoresis with the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) and the proportional amount of each fragment to the whole yield of splicing products was calculated ...
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bioRxiv - Bioengineering 2021Quote: ... mRNA was isolated from high quality total RNA samples with RIN > 9 and 28S:18S > 1 (measured with an Agilent 2100 Bioanalyzer) using poly-T oligonucleotide beads ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a 2uL aliquot from each plated sample was diluted 1:9 on a Bravo liquid handling platform (Agilent, Santa Clara, CA, USA). A 5 μL aliquot from each diluted sample was arrayed into a 384 well plate for input into the mNGS library prep ...
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bioRxiv - Plant Biology 2021Quote: ... at 37⁰C (1:50 protein/trypsin ratio) and cleaned up with a C18 column (OMIX C18, Agilent, Santa Clara, CA, USA). Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were loaded onto a ZORBAX SB-C18 column (150 x 1 mm, 3.5 μm, flow of 90 μl/min; Agilent, Les Ulis, France) heated at 40°C ...
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bioRxiv - Neuroscience 2021Quote: ... A capillary column was the stationary phase (30 m x 0.53 mm x 1 µm film thickness; Agilent Technologies, Santa Clara, CA), and helium was used as the mobile phase (at a flow rate of 8.5 mL/min) ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody detection was followed by 45 min of incubation with goat anti-mouse-biotin (DAKO, E0433, 1:200 in TNB blocking buffer) and subsequent incubation with Streptavidin-HRP Conjugate (PerkinElmer ...
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bioRxiv - Neuroscience 2019Quote: ... Endogenous peroxides were quenched with 1 % hydrogen peroxide in 10 % methanol for 10 minutes followed by blocking in antibody diluent (Dako /S 202230) for 10 minutes ...
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bioRxiv - Physiology 2019Quote: ... for 15 minutes and then incubated overnight at 4°C with primary antibodies diluted in DAKO antibody diluent: anti-Insulin (DAKO, 1:1000), anti-BRD4 (Bethyl laboratories ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Synthetic Biology 2020Quote: ... and was allowed to pass through the packed catalyst bed at space velocities in the range of 1-45 s−1 by helium as the diluent gas.44 The injection was immediately followed by separation using HP-PLOTQ column (Agilent, 19091P-QO4), and compounds in the effluent stream were identified using online Agilent 5975C Triple-Axis MS detector ...
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bioRxiv - Microbiology 2020Quote: ... Cells were next washed three times with PBS and incubated with a secondary fluorescein isothiocynate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200) in 1% BSA for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with Bond Wash buffer 3 x 30s and then incubated with either mouse anti-CD68 (M0814, 1:100, Dako, Carpenteria, CA), mouse anti-βIII-Tubulin (G7121 ...