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Citations for Agilent :
8151 - 8200 of 8609 citations for Cyclic GMP cGMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... by inserting synthesized coding sequence for full length hRpn13 (NM_007002.3) or for residues 1–279 between the BamHI and HindIII restriction sites of pCMV-3Tag-1a (Agilent Technologies, 240295). Unmodified pCMV-3Tag-1a was used as empty vector (EV ...
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bioRxiv - Biophysics 2021Quote: ... The samples were heated up with a ramp rate of 1 °C/min over a temperature range of 15-95 °C using the qPCR System MX 3005 P (Stratagene). Measurements were performed in triplicate ...
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bioRxiv - Biochemistry 2021Quote: Rat Kir6.1 and N-terminal FLAG-tagged (DYKDDDDK) SUR2B were first cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses in HEK293 cells according to manufacturer’s instructions63,64 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane produced following transfer was probed with MAb214 primary antibody and then visualized with anti-mouse horseradish peroxidase secondary antibody (1:1000; Dako) for 1 h.
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bioRxiv - Neuroscience 2021Quote: ... A corresponding anti-rabbit or anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody at a dilution of 1:7000 (Dako) or 1:2000 (Pierce ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections (50 μm thickness) were prepared by a microslicer (DTK-1000, Dosaka EM) and treated with 1 mg/mL pepsin (DAKO) in PBS containing 0.1% Triton X-100 and 0.2 N HCl (Wako ...
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bioRxiv - Microbiology 2020Quote: Proteins were detected using the following primary antibodies: anti-enterovirus VP1 clone 5-D8/1 antibody purchased from Dako (Denmark); Mouse anti-Ub ...
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bioRxiv - Microbiology 2020Quote: ... 1:5,000 anti-mouse (for anti-FLAG) or 1:5000 anti-rabbit (for all the others) linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... 1:5,000 anti-mouse (for anti-FLAG) or 1:5000 anti-rabbit (for all the others) linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... Tissue samples were stained with hematoxylin and eosin or immunostained to detect SARS-CoV-2 antigen using VIDO-InterVac’s polyclonal rabbit anti-SARS-CoV-2 AS20-014 (1:400 dilution) and detected using an HRP-labelled polymer detection reagent (EnVision+ System – HRP Labelled Polymer, Agilent Technologies Canada Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... sections were incubated for 2 hours at room temperature with polyclonal guinea pig anti-insulin antibody (1:5, Agilent Technologies). Slides were then washed 3 x 5 minutes with PBS and incubated with the Anti-guinea pig IgG Alexa Fluor 488 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... 5% or 10% milk overnight followed by 1 hour incubation with the indicated primary antibodies followed by HRP conjugated secondary antibodies (Dako) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were incubated overnight with primary antibodies (collagen I; rabbit anti-bovine IgG, Cederlane, Burlington, ON, dilution 1:500, collagen IV; mouse anti-human IgG, Dako, Carpinteria ...
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bioRxiv - Biophysics 2021Quote: ... cells were resuspended by 200 μL 1% (w/v) BSA in PBS for detection by a NovoCyte flow cytometry (Agilent).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subjected to antigen retrieval antigen retrieval (pH = 6.0 for cleaved caspase-3 and pH = 9.0 for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Cancer Biology 2021Quote: ... while pH = 9.0 was used for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Biochemistry 2022Quote: ... and meprin α R372T (100 μl at 0.44 mg ml−1) were applied onto an AdvanceBio SEC column (300 Å, 7.8×300 mm, Agilent Technologies). The data was evaluated using ASTRA® 6 (Wyatt Technology).
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bioRxiv - Molecular Biology 2022Quote: ... the blots were further incubated with a primary anti-total Tau antibody (rabbit Dako-A0024, 1:5000 in blocking buffer), then with the anti-rabbit HRP-conjugated secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... approximate ~1 ×105 TH17 cells were suspended in 50 μL assay medium (Seahorse XF RPMI Assay Medium, pH 7.4, Agilent Technologies) containing 10 mM glucose ...
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bioRxiv - Physiology 2022Quote: ... Membranes were washed with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: swine anti-rabbit Ig HRP-conjugated (1:3000, catalog no. P0399, Dako) or goat anti-mouse Ig HRP-conjugated (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were washed three times with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibodies (Dako). Proteins were detected by enhanced chemiluminescence reagents (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The plasma (50 µL) was diluted with 150 µL of buffer A (1:4 dilutions, as recommended by Agilent Technologies), and then filtered to remove particulates using a 0.45 μm spin filter (Spin-X centrifuge tube filter ...
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bioRxiv - Cancer Biology 2022Quote: ... Extracts precipitated using Mouse anti-Menin antibody were probed using Rabbit anti-GFAP antibody (Dako Agilent Technologies, #Z033429, 1:2000) whereas extracts precipitated with Rat anti-GFAP antibody were probed with Rabbit anti-Menin antibody (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Extracts precipitated using Mouse anti-Menin antibody were probed using Rabbit anti-GFAP antibody (Dako Agilent Technologies, #Z033429, 1:2000) whereas extracts precipitated with Rat anti-GFAP antibody were probed with Rabbit anti-Menin antibody (Bethyl Laboratories ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were incubated in 0.1% Triton with 2% goat serum in PBS at 4°C overnight: rabbit anti-GFAP (1: 1000, DAKO, Z0334), rabbit anti-IBA-1 (1 ...
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bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation for 45 minutes at room temperature in 1:100 dilution of rabbit anti-goat immunoglobulins/HRP or ready-to-use goat antirabbit HRP labelled polymer (Dako). The slides were washed twice in 1x Wash buffer and the color was developed with 3,3-diaminobenzidine tetrahydrochloride (DAB ...
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bioRxiv - Immunology 2020Quote: ... and were used directly in experiments or fractionated into CD45RA+ and CD45RO+ subsets using anti-CD45RA-PE (UCHL-1, Dako) and anti-PE magnetic beads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... SIV vpx start codon and two additional ATG codons upstream of the HIV-1 vpr start codon were mutated by Quickchange mutagenesis (Stratagene) and the sequence between the stop codon of vif and start codon of vpr were deleted ...
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bioRxiv - Molecular Biology 2020Quote: ... Converted libraries were enriched by 10 cycles of PCR with the following reaction composition: 1 μl Pfu TurboCx Hotstart DNA polymerase (Stratagene), 5 μl PfuTurbo Cx reaction buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Sensor plates were calibrated overnight in a CO2-free incubator at 37°C using 1 mL/well of XF calibrant solution (Agilent). After polarization ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Secondary antibodies used were goat anti-rabbit BrightVision Horse Radish Peroxidase (Klinipath, Duiven, The Netherlands) and goat anti-mouse (#A32723, dilution 1:5000) obtained from Dako products (CA ...
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bioRxiv - Neuroscience 2021Quote: ... we used horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies (diluted 1:200 in PBS; DAKO); staining for fluorescent immunohistochemistry was performed using donkey anti-mouse ...
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bioRxiv - Microbiology 2021Quote: ... 1 µL was injected in splitless mode through a GC inlet liner (ultra inert, splitless, single taper, glass wool, Agilent) onto a DB-5MS column (30 m × 0.25 mm ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS and incubated with a fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200). Cell nuclei were stained with 4’ ...
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bioRxiv - Physiology 2022Quote: ... for 1 h at RT and incubated overnight at 4°C with 1:100 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
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Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiaebioRxiv - Bioengineering 2019Quote: ... The dried samples were sent to the Profleader Institute for complex sphingolipids analysis and solubilized in dichloromethane-methanol (2:1,vol/vol) before analysis by UHPLC-QTOF-MS (Agilent) analysis (Fig ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µL was injected in splitless mode through a GC inlet liner (ultra inert, splitless, single taper, glass wool, Agilent) onto a DB-5MS column (30 m × 0.25 mm ...
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bioRxiv - Cancer Biology 2019Quote: ... 3-4 μm slides were cut and stained for MLH1 (Monoclonal Mouse Anti-Human; Clone ES05, dilution 1 : 10,Dako), MSH2 (Monoclonal Mouse Anti-Human ...
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bioRxiv - Biochemistry 2020Quote: UV-visible spectra were recorded in 1-cm optic path quartz cuvettes under aerobic conditions on a Cary 100 Uv-vis spectrophotometer (Agilent) and under anaerobic conditions in a glove box on a XL-100 Uvikon spectrophotometer equipped with optical fibers.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were incubated with 1:200 (v/v) mouse monoclonal anti-AX AO3.2 [16] and 1:2000 (v/v) anti-mouse IgG-HRP (Dako Cytomation) diluted in 1%(w/v ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 μL of this mixture was injected into an Agilent 7890A Gas Chromatograph with 5977A MSD (Agilent, Santa Clara, CA) using an Agilent HP-5MS (30 m length x 0.25 mm i.d ...
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bioRxiv - Cancer Biology 2019Quote: ... Devices were then washed with 1× PBS solution for 3 times and mounted on glass slides using fluorescent mounting medium (Dako) and the coverslips were sealed using nail polish ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
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bioRxiv - Genomics 2021Quote: ... Between 1 and 5 ng of the purified digested DNA was loaded on a High Sensitivity DNA chip (Agilent Technologies) and run on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Neuroscience 2020Quote: ... we used horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies (diluted 1:200 in PBS; DAKO) to bind the primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Stained cells suspensions were counted in 20 μl sample volume at medium flow rate (60 μl min−1) using an ACEA NovoCyte Green flow cytometer (OMNI Life Science Agilent). The SYBR Green I signal was measured in the FITC-channels of the instrument ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated from 25 °C to 96 °C at 1 °C/min using an Mx3005P qPCR machine (Agilent Technologies). The dye was excited at 492 nm ...