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Citations for Agilent :
751 - 800 of 982 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... rinsed with water and then blocked in a serum-free protein block buffer for 8 min (Dako, X0909). Primary CD133 antibody (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... All antibody conjugates were run on a Bioanalyzer Protein 230 electrophoresis chip (Agilent Technologies, cat. no. 5067-1517) to verify successful conjugation.
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bioRxiv - Neuroscience 2021Quote: ... we performed immunohistochemical stainings against glial fibrillary acidic protein (GFAP, rabbit-anit-GFAP, 1:500, Dako, Hamburg, Germany) counterstained with a Cy3-conjugated secondary antibody goat-anti-rabbit (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The peptides from proteins digested were desalted and concentrated with C18 reverse phase chromatography (OMIX C18, Agilent technologies) and peptides were eluted with 80% acetonitrile (ACN ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μl of a protein sample at 8.5-10 mg/ml was injected to a BioSEC3-300 (Agilent) or Superdex 75 10/300 GL increase column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... To generate mutant proteins these vectors were altered by using QuickChange® XL site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were expressed in and purified from BL21-CodonPlus(DE3)pLysS Escherichia coli (Agilent Technologies, Santa Clara, CA) as described 48 and dialyzed against PLS buffer ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a rinse with TBS and a 30-minute blocking step with the Dako Protein Block (Dako) at room temperature ...
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bioRxiv - Immunology 2022Quote: ... C antibody (clone W6/32) that was immobilized and covalently linked to Protein A cartridges (Agilent G5496-60000). Peptides were acid eluted from antibody bound ProteinA cartridges using 0.1M acetic acid/0.1%TFA followed by desalting with C18 solid-phase extraction (SPE) ...
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bioRxiv - Bioengineering 2023Quote: ... cryosections of the tissues were first permeabilized and blocked using a protein block solution (Dako, Carpinteria, CA, USA) containing 0.1% saponin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: The purified IMP2 protein +/− RNA was analyzed by SEC-MALS using an HPLC system (Agilent Technologies 1260 Infinity) connected to a MALS system (Wyatt DAWN HELEOS II Ambient with Optilab TrEX HC differential refractive index detector) ...
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bioRxiv - Immunology 2023Quote: SECMALS analysis of protein nanoparticles was performed on a 1260 Infinity II high-performance liquid chromatography system (Agilent) coupled with a miniDAWN and Optilab detectors (Wyatt Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were passively cooled to room temperature and then incubated in Protein Block Serum-Free Reagent (Agilent (Dako), Santa Clara ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were passively cooled to room temperature and then incubated in Protein Block Serum-Free Reagent (Agilent (Dako), Santa Clara ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2022Quote: ... 14,000 rpm on a tabletop centrifuge to remove aggregated proteins and then sequentially injected into a HPLC (Agilent) equipped with a C18 analytical column (Agilent Poroshell 120).
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bioRxiv - Biophysics 2022Quote: ... The UV–visible spectra of purified NQO1 proteins were measured in a Cary spectrophotometer (Agilent Technologies, Waldbronn, Germany) and used to quantify NQO1 concentration and the content of FAD as described in (12) ...
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bioRxiv - Synthetic Biology 2022Quote: The Cas12a library targeting the protein-coding genes in PO1f was ordered as an oligonucleotide pool from Agilent Technologies Inc ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Abs) against glial fibrillary acidic protein (GFAP, rabbit anti-GFAP (1:2000, Dako, Z0334, RRID: AB_10013382)) and neuronal nuclear antigen (NeuN ...
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bioRxiv - Immunology 2023Quote: ... Antigens were tetramerized by incubation at a >4:1 ratio of biotinylated protein with streptavidin-PE (Agilent; PJRS25) or streptavidin-APC (Agilent ...
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bioRxiv - Neuroscience 2023Quote: Free-floating brain tissue was stained for polyclonal rabbit anti-glial fibrillary acidic protein primary antibody (GFAP; DAKO, Santa Clara ...
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bioRxiv - Pathology 2024Quote: ... The samples were then desalted with C18 tips with the AssayMAP Bravo Protein Sample Prep Platform (Agilent Technologies), by using the Peptide Clean-Up program ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were revealed by chemiluminescence (Super Signal West Dura Substrate, Pierce) using a secondary peroxydase-conjugated antibody (Dako) at a dilution of 1:10000 ...
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bioRxiv - Synthetic Biology 2024Quote: The Cas9 library targeting the protein-coding genes in PO1f was ordered as an oligonucleotide pool from Agilent Technologies Inc ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibodies used were rabbit anti–glial fibrillary acidic protein (GFAP; 1:250, Agilent Cat# Z0334, RRID:AB_10013382); goat anti–ionized calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2024Quote: GST-fusion proteins were expressed in BL21-Codon Plus strain of Escherichia coli cells (Agilent, Santa Clara, CA) and purified with glutathione-Sepharose beads (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... 17 500 ng of total protein was injected onto a home-packed PLRP column (PLRP-S) (Agilent Technologies), 10 μm particle size ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were initiated by addition of protein and monitored using a Cary Eclipse fluorometer (Agilent Technologies, Mississauga, Canada). Cleavage of Z-VLR-AMC was monitored using 380 nm and 450 nm excitation and emission wavelengths ...
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bioRxiv - Cancer Biology 2024Quote: ... After elution bound proteins were enzymatically digested with trypsin using an AssayMAP Bravo liquid handling system (Agilent technologies) running the autoSP3 protocol as described here77 ...
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bioRxiv - Plant Biology 2020Quote: ... The sample components were separated on a C18 olumn (Hypersil GOLD C18; 4.6 mm × 100 mm; 3 µm particle size; Agilent, Santa Cruz, CA, USA). The column was eluted at 0.3 mL/min with a gradient from 95% solvent A (0.1% formic acid in water ...
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bioRxiv - Developmental Biology 2022Quote: ... High quality total RNA (RNA integrity number = 10) was labelled with Cyanine 3 CTP using the Low Input Quick Amp Labelling Kit (Agilent Technologies-5190-2305), and purified using Qiagen’s RNeasy Mini Spin Columns ...
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bioRxiv - Biochemistry 2022Quote: ... All libraries at this step yielded >300,000 colonies implying a 100x coverage (assuming 1/3 of the variants were perfect based on our previous analysis of Agilent OLS based libraries). Each sublibrary was combined at an equimolar ratio to make a complete library with all intended mutations included.
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bioRxiv - Microbiology 2021Quote: ... The extracts were acidified to pH 2 and additionally pre-treated with solid-phase extractions using Agilent Bond Elut-PPL 3 mL columns and diluted to 50 ppm (Agilent Technologies, DE, USA) following standard lab protocol29 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibodies were diluted 1:4000 for anti-sGCα1 and 1:2000 for anti-sGCβ1 antibody in 3% dry milk in TBST and incubated with nitrocellulose membranes at 4°C over-night following challenge of membranes with secondary goat anti-rabbit antibody (1:2000 in 3% milk in TBST) conjugated to horseradish peroxidase (Dako A/S, Denmark). Immuno-complexes were visualized using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in micebioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared using an automated - QuantSeq 3’mRNA-seq (Lexogen GmbH, Vienna) and Agilent NGS Workstation (Agilent Technologies, Santa Clara) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Biochemistry 2022Quote: 50 μL of pure CrRPE1 concentrated at 9.5 mg mL−1 were injected on BioSEC-3 300 size-exclusion chromatography column (Agilent Technologies, Santa Clara, USA) equilibrated in buffer 20 mM Tris-HCl (pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Plant Biology 2024Quote: ... For separation of all metabolites, we used a Zorbax RRHD Eclipse XDB-C18 column (50 x 3 mm, 1.8 µm) (Agilent, Santa Clara, CA, USA) with a 1290 Infinity II inline filter (0.3 µm ...
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bioRxiv - Immunology 2024Quote: ... The antigens on the slides were retrieved by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent, Cat# S2375) and incubating at 97 °C for 40 min in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... and endogenous peroxidase was blocked with 3% H2O2 in TBS for 5 min followed by incubation with anti-mouse EnVision+ labelled polymer (Agilent Technologies, Santa Clara, USA) for 30 min ...
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bioRxiv - Immunology 2022Quote: Naive CD3+ T cells were placed on PLL-coated Seahorse Bioanalyzer XFp culture plates (3 × 105 cells/well) with Seahorse XF RPMI Assay Media (RPMI Medium, pH 7.4, 103576-100; Agilent Technologies, Santa Clara, CA, USA), supplemented with 10 mM glucose (103577-100 ...
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bioRxiv - Cell Biology 2022Quote: ... were diluted with ultra-pure water to reduce the acid concentration below 3% and loaded into the ICP-MS-MS (triple quad Agilent 8800x ICP-MS-MS). The instrumental conditions were optimized to remove interferences by using a collision/reaction cell ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Physiology 2021Quote: ... was amplified by PCR using cDNA of mouse kidney at the template with the primers 5′-CCGTCGACATGCAGGTCAAGAAATCCTG-3′ (SalI site in italics) and 5′-CCGGATCCTTAAAGGCGTGTGTGATCTT-3′ (M6 reverse primer; BamHI site in italics) and cloned into pBluescript SK(−) (Stratagene, La Jolla, CA, USA) using the SalI and BamHI sites ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For phthalates and BPA extraction we used Agilent Vac Elut Manifold with SPE cartridge C18 ODS 3 ml tubes 200 mg (Agilent Santa Clara CA, USA). SPE cartridges were conditioned with 2 ml of methanol and then 2 ml of MilliQ water ...
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bioRxiv - Synthetic Biology 2023Quote: Samples (3 µL) were injected into an ion mobility drift tube-quadrupole time-of-flight mass spectrometer (LC-IM-QTOF, Agilent 1290 series LC 6560). An Agilent Poroshell 120 HILIC-Z 150 × 2.1 mm ...