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Citations for Agilent :
7751 - 7800 of 8609 citations for Cyclic GMP cGMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA, USA, 5067-1511). High-Quality RNA samples with RIN number > 7.0 were used to construct the sequencing library ...
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bioRxiv - Cell Biology 2023Quote: ... Dephosphorylated and denatured total RNA was labeled with cyanine 3-pCp and subsequently hybridized to the Agilent Mouse microRNA Microarray Release 15.0 using the microRNA Complete Labeling and Hyb Kit (Agilent Technologies, Inc., Santa Clara CA). Following hybridization for 20 h ...
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bioRxiv - Cell Biology 2022Quote: ... The quality of the cDNA product was checked using an Agilent High Sensitivity DNA Kit and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) to ensure the complete removal of contaminants ...
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bioRxiv - Biochemistry 2023Quote: ... The cDNA of Affilin variant Af2p was used as template for error-prone PCR employing the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA, USA). The error rate was set to 10-14 mutations per kbp and the resulting theoretical library size was calculated to contain 6·1010 independent variants ...
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bioRxiv - Cell Biology 2023Quote: ... The size distribution and concentration of the libraries were assessed on Tapestation with a DNA High Sensitivity kit (# 5067-4626, Agilent Technologies, Santa Clara, CA). Paired end 38 bp sequences were generated from samples on a NextSeq 500/550 (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... A mutant Acr library was made using ACX175 as a backbone and error-prone PCR with low mutation rate using GeneMorph II random mutagenesis kit (Agilent, Santa Clara, CA, USA). The resulting library was subcloned into a second plasmid containing AiEvo2 ...
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bioRxiv - Cell Biology 2023Quote: ... S781A or S781D) were created by standard mutagenesis using QuickChange II XL Site-Directed Mutagenesis Kit (cat#200522, Agilent Technologies, Santa Clara, CA, USA). CDS of full-length Ctb ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 expression was assessed using the US Food and Drug Administration-approved Dako PD-L1 IHC 22C3 pharmDx kit (Agilent Technologies, Santa Clara, CA) in the samples ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with FSCN1 primary antibodies at 4°C overnight and the color of the sample was developed using the DAKO ChemMate Envision Kit HRP (Dako-Cytomation, Carpinteria, CA, USA) followed by counterstaining with hematoxylin ...
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bioRxiv - Neuroscience 2023Quote: ... and the average size of the libraries was determined using the High Sensitivity NGS fragment analysis kit on the Fragment Analyzer (Agilent Technologies; Santa Clara, CA). The normalized libraries were then sequenced on an Illumina NextSeq500 sequencing platform with 75-bp single-end reads for at least 20 million reads per sample in multiplexed sequencing experiments.
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bioRxiv - Neuroscience 2023Quote: ... Libraries concentrations and size distributions were assessed by running samples on Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit (Agilent, Part Number 5067-4626). cDNA sample size modes were about 300 nucleotides (N.B. ...
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bioRxiv - Genetics 2023Quote: ... The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA, USA, 5067-1511). High-Quality RNA samples with RIN number > 7.0 were used to construct the sequencing library ...
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bioRxiv - Neuroscience 2023Quote: ... labelled cRNA was prepared from 200 ng (mPFC/HT) or 40 ng (NAc) of total RNA using the One-Color Quick Amp Labelling kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... A panel of SUMO3-CXCL17 truncation mutants was generated by SDM by introducing stop codons at the required positions using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies; Santa Clara, USA).
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bioRxiv - Cancer Biology 2023Quote: ... SCR_019417) was used for library size distribution analysis with a high-sensitivity NGS Fragment Kit (Cat# DNF-474-0500, Agilent, Santa Clara, CA, USA). qPCR for quantification was run using an Illumina-compatible KAPA Library Quantification Kits (ROX Low ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A subset of the samples from each developmental stage was quality-checked by Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies Catalog # 5067-1513). The resulting RNA integrity numbers were between 8.4 and 10 ...
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bioRxiv - Genomics 2023Quote: ... The quality of the cDNA libraries and final DNA libraries were analyzed using the 2100 Bioanalyzer Instrument with the High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA).
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bioRxiv - Pathology 2023Quote: ... RNA concentration was determined using an RNA 6000 Pico Kit run on a Bioanalyzer 2100 running Agilent 2100 Expert Software (B.02.11; Agilent Technologies, Santa Clara, CA, USA). RNA was converted to cDNA using iScript cDNA Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The hybridization was performed according to the manufacturer instructions on 4×180K mouse microarrays (SurePrint G3 Mouse CGH Microarray Kit, 4x180K, AGILENT Technologies, reference genome: mm9). Microarrays were scanned with an Agilent High-Resolution C Scanner using a resolution of 3 µm and the autofocus option ...
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bioRxiv - Physiology 2023Quote: ... Standard curves for each gene were generated by cloning qPCR products into the pSCA vector with the Strataclone PCR cloning kit (Agilent, Santa Clara, CA, USA), isolating plasmid DNA using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Aliquots of ∼0.1 g were used for total RNA (RNAt) extractions with the Agilent Plant RNA isolation Mini kit (Agilent Technologies, Santa Clara CA, USA). Three aliquots of RNAt per sample were separated and their concentrations adjusted to 50 ng μL−1.
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bioRxiv - Neuroscience 2023Quote: ... Quality control of total RNA quality and quantity was done on the Agilent 2100 Bio analyzer (Agilent RNA 6000 Nano Kit, Cat# 5067-1511) QC ...
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bioRxiv - Cancer Biology 2024Quote: ... Extracts from tumors and media were analyzed using the Absolute IDQ p180 kit (Biocrates Life Sciences AG, Innsbruck, Austria) with an Agilent 1290 Infinity UHPLC system (Agilent, Santa Clara, CA, USA) coupled with an MS/MS Sciex Triple Quad 6500 (AB Sciex LLC ...
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bioRxiv - Plant Biology 2024Quote: ... concentration and integrity were evaluated on 0.8% agarose gels (v/w) and with the Bioanalyzer RNA Nano 6000 Assay Kit (Agilent Technologies, Santa Clara, CA, USA). RNA sequencing was performed using Illumina NovaSeq 6000 platform ...
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bioRxiv - Immunology 2023Quote: ... The oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) were measured with an XF Cell Mito Stress Kit (Agilent, Santa Clara, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Basal ATP production rates were calculated using the Seahorse XF Real-Time ATP Rate Assay Kit Report Generator (Wave Desktop Software, Agilent Technologies, Cedar Creek, TX). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The quality and concentration of both cDNA and libraries were assessed using an Agilent BioAnalyzer with High Sensitivity kit (#5067-4626, Agilent, Santa Clara, CA USA) and Qubit Fluorometer with dsDNA HS assay kit (#Q33230 ...
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bioRxiv - Cell Biology 2023Quote: Completed sequencing libraries were quantified using the Qubit Fluorometer and the Qubit dsDNA HS Assay Kit and fragment length distributions assessed using the Agilent 4200 TapeStation and the High Sensitivity D1000 ScreenTape Assay (Agilent; 5067-5584, 5067-5585).
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bioRxiv - Plant Biology 2023Quote: ... isolated and DNAse treated RNA extracted from 5-week-old plants was assayed for RNA integrity with the Agilent 2100 Bioanalyzer using the RNA Nano 6000 kit (Agilent Technologies, Santa Clara, USA). Single-end RNA-seq was performed on a HiSeq 2500 High Output V4 platform (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids harboring mutated sRNA alleles under the control of the PLlac promoter were constructed using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) with oligonucleotides that contain mismatched bases at the desired locations (Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Point mutation of D5R-STOP from D5-TANGO was made by polymerase chain reaction (PCR) using the QuikChange II Site–Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... Point mutations were induced using either the QuikChange II XL Site-Directed Mutagenesis or Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... the cDNA was purified by SPRIselect Reagent (Cat# B23318) and quantified using Picogreen™ dsDNA assay kit (Cat# P7589) and quality assessed using High Sensitivity D5000 ScreenTape (Agilent, Cat# 5067-5592) with High Sensitivity D5000 Reagents (Agilent ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Aliquots of ∼0.1 g were used for total RNA (RNAt) extractions with the Agilent Plant RNA isolation Mini kit (Agilent Technologies, Santa Clara CA, USA). Three aliquots of total RNA (RNAt ...
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bioRxiv - Neuroscience 2024Quote: ... The library quality was evaluated by measuring the library size in base pairs using the Agilent High Sensitivity DNA Kit and Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). During the library construction ...
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bioRxiv - Cell Biology 2024Quote: ... Sample amplification and labeling were carried out using the Low Input Quick Amp labeling Kit (Catalog number 5190-2305, Agilent Technologies, Santa Clara, CA). Briefly ...
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bioRxiv - Immunology 2024Quote: RNA from splenocytes isolated from UVB exposed WT and Trim21-/-mice were analyzed for RNA integrity on the 2100 Bioanalyzer using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA) and quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... We used the following antibodies for the murine sections: anti-GFAP (1:500, Dako, raised in mouse), anti-NeuN (1:150 ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with rabbit anti-mouse horseradish peroxidase (HRP) conjugated antibody (P026002-2, Dako, 1:1000) as secondary antibody at RT for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... antigen retrieval and stained with a prediluted anti-Ki-67 antibody (Mouse, Clone MIB-1, Dako #M7240) using an automated immunostainer ...
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bioRxiv - Physiology 2020Quote: ... and then incubated with polyclonal rabbit antibody raised against human von Willebrand Factor (1:500; A0082, Dako), at 4°C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were processed as free-floating and stained with rabbit polyclonal anti-GFAP (1:2000 dilution, DAKO Z0334 ...
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bioRxiv - Bioengineering 2019Quote: ... Hydrogels were incubated in a 1:100 dilution mouse anti-human CD31 primary antibody (Agilent IS61030-2) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used were: mouse anti-human CD8 (Dako, clone C8/144B, 1:800, pH 9 retrieval), mouse anti-human CD68 (Dako ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Sections were incubated with primary antibodies as follows: Mouse anti-CD31 antibody (1:20; REF MD0823, Dako), Rabbit anti-CD90 antibody (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies were conjugated to HRP and used in dilutions of 1:10000 (goat α-mouse, Dako) and 1:3000 (goat α-guinea pig ...
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bioRxiv - Cancer Biology 2021Quote: ... the plasma was diluted 4:1 with Buffer A for Multiple Affinity Removal LC Columns (Agilent Technologies) and filtered through a 0.22 μm hydrophilic PVDF membrane filter plate (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary incubations were performed with horseradish peroxidase-linked polyclonal goat anti-rabbit (1:5000; Dako, Glostrup, Denmark) or polyclonal rabbit anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with secondary horseradish peroxide (HRP)-conjugated goat anti-rabbit antibodies (Dako P0448, 1:1000) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... and covered with glass coverslip of #1 thickness (Carl Roth GmbH NK79.1) using mounting media (Dako S3023). The sections were imaged on Zeiss LSM 780 confocal microscope ...