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Citations for Agilent :
7651 - 7700 of 8609 citations for Cyclic GMP cGMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... β-catenin was detected using an anti-human β-catenin antibody (1:1000) (Agilent Dako, USA). After three washes in 1X PBS – 0.3% Tween 20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used were: Goat anti-mouse IgG HRP (Cat no. P0447, Dako, 1:1500 dilution) and Goat anti-rabbit IgG HRP (Cat no ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was immunoprobed with the polyclonal anti-TTR antibody (DAKO, ref. A0002, dilution 1:1000) and then incubated with alkaline phosphatase-conjugated polyclonal anti rabbit secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with Cgrp antibody (rabbit polyclonal, abcam #ab47027, 1:300 in DAKO antibody diluent (Agilent, #S0809)) ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a ...
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bioRxiv - Pathology 2023Quote: ... followed by another 30 min room temperature incubation with streptavidin/horseradish peroxidase (1:300, P0397, Agilent). Staining was obtained by using an alcohol-soluble peroxidase substrate ...
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bioRxiv - Cell Biology 2023Quote: ... Both membrane parts were incubated with a rabbit-anti-mouse-(1:1000; P0261; DAKO, Agilent, USA) and swine-anti-rabbit-HRP conjugated secondary antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Both membrane parts were incubated with a rabbit-anti-mouse-(1:1000; P0261; DAKO, Agilent, USA) and swine-anti-rabbit-HRP conjugated secondary antibody (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody incubation was performed in polyclonal rabbit anti mouse – HRP antibody 1:2000 (Dako, P0260) or polyclonal goat anti rabbit – HRP antibody 1:2000 (Dako ...
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bioRxiv - Cell Biology 2023Quote: ... we performed immunochemistry assays using mouse anti- human ki67 antibody (M7240, DAKO, 1/200 at pH9) in a series of paraffin-embedded tissue blocks of HGSOC ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation with the primary antibody (diluted 1:3,000 in dilution buffer; Dako) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... the tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After washing with PBS the aortic tissue sections were specifically stained with primary antibodies for platelet GPIbα (CD42b #M042-0 ...
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bioRxiv - Cancer Biology 2023Quote: ... of which 1 μl was examined on a Bioanalyser high sensitivity DNA chip (Agilent 5067–4626). Ideal cycle number should bring final library to final concentration of 1 to 3 nM ...
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bioRxiv - Immunology 2023Quote: ... Tetramers were assembled by conjugating the biotin-labeled monomers with PE-labeled streptavidin (Agilent, #PJRS27-1) according to NIH Tetramer Facility protocols ...
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bioRxiv - Cell Biology 2022Quote: ... samples were exposed to antigen retrieval (10 minutes at 105°C in 1 x TRS (DAKO)) ...
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bioRxiv - Plant Biology 2023Quote: ... equipped with a DB-1 capillary column (15 m/0.25 mm/0.25 µm, Agilent J&W) and a mass selective detector (Agilent 5975C MSD ...
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bioRxiv - Molecular Biology 2023Quote: ... and the quality was assessed using Femto Pulse at 1 mg/mL (Agilent, Santa Clara, CA).
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bioRxiv - Cancer Biology 2023Quote: ... Electrical impedance was measured for 260 intervals of 1 hour using the RTCA xCELLIgence device (Agilent). Cell index slope and doubling time were retrieved from the software and used for statistical analysis in R.
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... Immunohistochemistry (IHC) for Ki-67 antigen was performed using the Ki-67 Antibody (MIB-1, Dako) at 1:100 as we have previously described (79) ...
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bioRxiv - Microbiology 2023Quote: ... were cloned into the pShuttle (PS-1) vector of the AdEasy adenoviral vector system (Agilent Technologies). The entire cassette was amplified with the primers CRISPRcut_PS1_fw and CRISPRcut_PS1_rev and assembled with the KpnI digested PS-1 plasmid using In-Fusion® HD Cloning ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at room temperature and probed with primary antibodies diluted in antibody diluent (Dako) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated with Hoechst (1:1500) for 5 minutes and mounted with Fluoromont (Dako) after 3 baths of PBS.
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bioRxiv - Neuroscience 2024Quote: ... were adjusted to 1 μg/ml in background-reducing antibody diluent (Agilent, Santa Clara, CA, USA) and applied overnight at 4 °C in a humidified chamber ...
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bioRxiv - Neuroscience 2024Quote: ... Immunohistochemistry was performed against glial fibrillary acidic protein (GFAP, 1:250, Agilent, Cat# Z0334, RRID: AB_10013382), ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Plant Biology 2024Quote: ... Standards of 1 μl derivatized sample was injected into GCMS (Agilent 6890-5975, Santa Clara, CA), with the inlet temperature of 250°C ...
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bioRxiv - Microbiology 2024Quote: ... was diluted 1:400 in antibody diluent with background reducing components (Agilent, Santa Clara, CA, US). Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... the incubation with a respective secondary antibody conjugated to horseradish peroxidase (1:10000 in PBST, DAKO) for 1 hour at room temperature was performed ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 8.2-10 for all samples ...
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bioRxiv - Genetics 2021Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 7.3–10 for all samples ...
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bioRxiv - Developmental Biology 2020Quote: ... analysis was performed using TransStart Tip Green qPCR SuperMix kit (TransGen Co.) on a Stratagene Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) under the conditions of 94 °C for 30 s ...
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bioRxiv - Genomics 2019Quote: ... and successful size-selection verified by electrophoresis on an Agilent TapeStation using the Genomic DNA High ScreenTape kit (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Standard quality control steps were included to determine total RNA quantity and quality using Agilent 2100 Bioanalyzer with Eukaryote Total RNA Pico Kit and High Sensitivity DNA Assay (Agilent Technologies, Santa Clara, CA). Libraries were prepared using the TruSeq Stranded mRNA Library preparation Kit (Illumina Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... early and late nascent strands were labeled with Cy3 and Cy5 ULS molecules using the Genomic DNA labeling Kit (Agilent, Santa Clara, CA, USA) following manufacturer's instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmid DNA mutations in the predicted orthosteric binding site of PAR4 were generated using the QuikChange XL Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Physiology 2019Quote: ... RNA quality and integrity were validated using a NanoDrop ND-1000 UV–vis Spectrophotometer (NanoDrop Technologies, Wilmington, USA) and Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, USA) respectively ...
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bioRxiv - Immunology 2019Quote: Various N-linked glycosylation site mutants of UL144 were generated by site-directed mutagenesis using Quick Change II site directed mutagenesis Kit (Stratagene, La Jolla, CA, USA). The Asn residues carrying the N-linked glycan were mutated individually or in combination at residues N61 ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatants were transferred to new tubes and 50 µl of each cleared lysate was mixed with 50 µl Lysis Buffer (0.7 µl β-mercaptoethanol/100 µl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at –80° for later preparation as input RNA ...
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bioRxiv - Genomics 2021Quote: ... Libraries from all sample sets were quantified on the 2100 Bioanalyzer instrument with the Agilent DNA 7500 Kit (both Agilent Technologies, Santa Clara, CA).
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bioRxiv - Bioengineering 2022Quote: ... Size pattern was assessed with Fragment Analyzer-96 with the DNF-474-0500 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies, DNF-474-0500). Libraries (Average size ...
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bioRxiv - Biochemistry 2021Quote: Mutated forms of rat and human BDH2 enzymes (Y157F and Y147F, respectively) were generated by site-directed mutagenesis using a QuikChange II XL kit (Stratagene, La Jolla, CA, USA), with pCOLD I/rBDH2 and pCOLD I/hBDH2 plasmids as templates and mutagenic primers Y157F-rBDH2-S and Y157F-rBDH2-AS as well as Y147F-hBDH2-S and Y147F-hBDH2-AS ...
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bioRxiv - Neuroscience 2020Quote: ... the purity and concentration of RNA samples were determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and Ile-residues of the HXXXDX motif of ZmGL2 and ZmGL2-LIKE were generated using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The ZmGL2 protein was also expressed in E ...
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bioRxiv - Systems Biology 2020Quote: ... The sub-libraries were each resuspended to a final volume of 20 μL in nuclease-free water and fragment distribution was checked running a bioanalyzer (Agilent High Sensitivity DNA Kit) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... The fragment sizes and concentrations of the pooled samples were determined on a Fragment analyzer 5200 using the DNF-473-Standard Sensitivity NGS Fragment Analysis Kit (Agilent, Santa Clara, CA, USA). The indexing PCR was performed under the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equal quantity and combined to multiplex to make a final library and QC was done again using Qubit quantification kit and Agilent bioanalyzer using high sensitivity DNA chip (Agilent Biotechnology, catalog # 5067-4626). The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using High output 300-bp Single end Read sequencing kit.
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bioRxiv - Plant Biology 2021Quote: ... Other related alleles required for transient expression or infection analyses were either created using the QuickChange Site-directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) or synthesised by Twist Bioscience (South San Francisco ...
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bioRxiv - Neuroscience 2022Quote: ... with the standard-sensitivity RNA chip or an Agilent 2100 Bioanalyzer system with the RNA 6000 Nano Labchip Kit (Agilent Biotechnologies, Palo Alto, USA) was used to analyze the extracted RNA samples ...
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bioRxiv - Genomics 2022Quote: ... The obtained library quality and concentration was assessed by monitoring the distribution of the fragment sizes with a Bioanalyzer 2100 instrument using RNAnano reagent kit (Agilent Technologies, Santa Clara, CA). This size and quantity information was used for pooling the libraries in equimolar concentrations to normalize each library ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA quality was assessed by determining RNA integrity numbers (RINs) using the Agilent 2100 Bioanalyzer system with the Agilent RNA 6000 Nano Chip Kit (Agilent Technologies, Santa Clara, California).