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Citations for Agilent :
7451 - 7500 of 9205 citations for Mouse Autophagy related protein 16 1 ATG16L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and PcrA V448C were generated by site-directed mutagenesis of the wild type construct using the Quikchange II XL kit (Agilent Technologies). In the last gel filtration chromatography of PcrA V448C ...
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bioRxiv - Genomics 2021Quote: ... Gene-specific probes were generated by random priming in the presence of ATP [α32P] using the Prime-It II Random Primer Labeling Kit (Agilent, 300385) using PCR generated DNA template produced from gDNA isolated from a wild type S.pombe strain (YP71 ...
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bioRxiv - Genomics 2021Quote: ... and library profile was assessed using a High Sensitivity DNA kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was sequenced on an Illumina NovaSeq instrument (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... The Drich and Reverse Drich CP mutants were made using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA). For the Drich mutant ...
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bioRxiv - Microbiology 2021Quote: Mutation of E2 residues 399YAL401 to AAA was achieved using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA). The following primers were used ...
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bioRxiv - Genomics 2021Quote: ... The libraries were quantified and qualified using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA analysis kit (Agilent, 5067-4626).
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bioRxiv - Cell Biology 2020Quote: ... residues at positions 29 and 30 and lysine residues at positions 33 and 34 to alanine (A) using the QuikChange Site-directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... To construct a plasmid encoding GAS6 tagged with Myc-His (pSecTag2-hGAS6-MycHis) the stop codon from pSecTag2-hGAS6 was removed using the QuickChange II XL Site-Directed Mutagenesis kit (Agilent Technologies). Next ...
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bioRxiv - Genomics 2021Quote: ... Final libraries were evaluated using fluorescent-based assays including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100). Libraries were sequenced on an Illumina NovaSeq 6000 system using 2 x 150bp cycles.
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bioRxiv - Neuroscience 2020Quote: ... Primary myotubes underwent assessment of key parameters of glucose and fatty acid oxidation dependency and capacity using the Seahorse Mito Fuel Flex Test Kit (Agilent Technologies).
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bioRxiv - Neuroscience 2020Quote: The dependence and capacity of EDL fibers to use glucose and fatty acid as fuel substrates was determined using the Seahorse XF Mito Fuel Flex Test Kit (Agilent Technologies). Prior to the assay ...
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bioRxiv - Neuroscience 2020Quote: ... The integrity and concentration of the extracted RNA were assessed using an Agilent RNA 6000 Nano Kit on a 2100 Bioanalyzer (Agilent, USA). Based on RNA integrity number (RIN > 7.2 ...
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bioRxiv - Microbiology 2021Quote: Mutations in AdMarRars were introduced by site-directed mutagenesis using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The mutagenic oligonucleotides used for both strands and the respective changes introduced (underlined ...
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bioRxiv - Cancer Biology 2022Quote: ... An aliquot of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Agilent).
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bioRxiv - Cancer Biology 2022Quote: ... Finally their fragment size was analyzed by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Agilent). 2×150 bp paired-end reads were sequenced on an Illumina HiSeq 2500 with a target depth of 60 million reads per sample.
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bioRxiv - Biochemistry 2020Quote: ... The mutations to DrBphP (H533D, E536A) and for AgP1 (D529H) were introduced with QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies). For cloning the chimera construct ...
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bioRxiv - Bioengineering 2020Quote: ... respectively in ThuE were designed by site-directed mutations in optical sucrose sensors by using the QuickChange II Site-Directed Mutagenesis (SDM) Kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Histone H3 bearing K36 substituted with cysteine (K36C) was generated by site-directed mutagenesis using an Agilent Quikchange XL Site-Directed Mutagenesis kit (Agilent #200517) with the canonical H3 gene in pET3 vector ...
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bioRxiv - Microbiology 2021Quote: ... (labeled regions A and B) were generated using the primer sets and cycle conditions indicated in Table S1B (EasyA PCR kit (Agilent Tech) and BioRad T100 thermal cycler) ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA integrity was evaluated using the RNA Nano 6000 Assay Kit for a Bioanalyzer 2100 System (Agilent Technologies, CA, USA). The complete circRNA study involved the following steps ...
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bioRxiv - Molecular Biology 2020Quote: ... pET29b-PyoS8-ImS8 was used as a template for the site-directed mutagenesis (QuikChange XL site-directed mutagenesis kit; Agilent Technologies) to yield pET29b-PyoS8Glu736Ala-ImS8.
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bioRxiv - Cancer Biology 2021Quote: Comparative gene expression profiling of the prostate cancer cells starved for glutamine for 24 h or cultured with glutamine supplementation was performed using SurePrint G3 Human Gene Expression 8×60K v3 Microarray Kit (Design ID 039494, Agilent Technologies) according to manufacturer’s recommendations as described previously 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Compound incubated plates and basal plates were then washed with assay media (Seahorse XF DMEM assay media kit (Agilent; #103575-100), 10 mM glucose ...
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bioRxiv - Cell Biology 2021Quote: ... except that in this case the cDNA was synthesized from 1 µg (testis) or 13-20 ng (spermatozoa) of RNA using the AccuScript High-Fidelity 1st Strand cDNA Synthesis Kit (Agilent 200820) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
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bioRxiv - Physiology 2021Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies), according to the manufacturer's instructions ...
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bioRxiv - Physiology 2020Quote: ... by site-directed mutagenesis of a pCI-NaV1.5 plasmid containing the human NaV1.5 hH1C cDNA (30) (NCBI Reference Sequence NM_000335) using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Sant Clara, CA) or the Q5 Site-Directed Mutagenesis kit (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... CA- and DN-RABG3 variants were generated by site directed mutagenesis with QuickChange Lightning II Site-Directed Mutagenesis Kit (Agilent Technologies) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA and libraries were made using the Ovation® RNA-Seq System V2 kit (NuGen) and quality was assessed by Agilent High Sensitivity DNA kit on a Bioanalyzer (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... two arginines at position 682 and 683 were changed to alanines using the QuickChange II XL site-directed mutagenesis kit (Agilent Technologies) and the plasmid pIB-spike-V5 as a template ...
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bioRxiv - Cell Biology 2021Quote: ... A gene fragment encoding three copies of the 9-amino-acid HA epitope followed by EcoR1 and XbaI restriction sites was inserted using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent technologies). The resulting GSPK-HA transgene plasmid (13,468 bp ...
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bioRxiv - Cell Biology 2021Quote: The oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) were measured using the MitoStress Test kit (Agilent, #103010–100) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... We performed mutagenesis with the primers (Table S1) designed for the R397W mutation according to the manufacturer’s instruction (QuikChange II Site-Directed Mutagenesis Kit, Agilent Technologies, USA). Upon reaching 50-60% density ...
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bioRxiv - Cell Biology 2021Quote: Site-directed mutations of the phosphorylation sites were created using similar methods as the Quickchange® II XL kit from Agilent technologies by using the primers listed below to recreate the whole vector via PCR with the new mutation in place ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR analysis of gene expression was undertaken using the KAPA SYBR FAST qPCR Kit and Stratagene Mx3005P qPCR System (Agilent Technologies). Thermal cycling conditions were as follows ...
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bioRxiv - Plant Biology 2020Quote: ... RNA quality was evaluated using the Agilent RNA 6000 Nano Kit on the Bioanalyser 2100 (Agilent Technologies, Santa Clara, CA, USA). Only samples with an RNA integrity number ≥ 7.0 were kept for library construction ...
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bioRxiv - Plant Biology 2020Quote: ... RNA quality was assessed using an Agilent RNA 6000 Nano Kit with the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Only samples with an RNA integrity number ≥ 7.0 were kept for library construction ...
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bioRxiv - Microbiology 2020Quote: ... RNA was processed and labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies), according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... The integrity of ribosome-depleted RNA was assessed using an Agilent RNA 6000 Pico Assay kit on the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA-seq libraries were constructed using ScriptSeq™ v2 RNA-Seq Library Preparation kits (Epicentre Biotechnologies ...
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bioRxiv - Microbiology 2021Quote: ... Sizes of indexed amplicons were verified using the High Sensitivity DNA kit on an Agilent 2100 Bioanalyzer system (Agilent Technologies, Canada). Indexed amplicons were pooled in equimolar amounts and sequenced on an in-house Illumina MiSeq benchtop sequencer (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA (100 ng) was labelled with Cy5 or Cy3 using the Two-Color Low Input Quick Amp labelling Kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was next converted to cDNA by using a polydT primer and following the AffinityScript Multiple Temperature cDNA Synthesis kit instructions (Agilent Technologies). In order to quantify the HEV genome ...
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bioRxiv - Immunology 2019Quote: ... Fragment sizes were confirmed using either the High Sensitivity DNA assay or the DNA 1000 kit and 2100 bioanalyzer (Agilent Technologies). Libraries were quantified with qPCR ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were then validated on a Fragment Analyzer system with a High Sensitivity NGS Fragment Kit (Agilent Technologies, Mississauga, ON, Canada) to check for size and quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... or turboGFP-MCMBPΔNLS (bipartite NLS was identified using cNLS Mapper35 generated by site directed QuickChange II XL Site-Directed Mutagenesis kit (Agilent, 200522) with primers (forward ...
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bioRxiv - Neuroscience 2020Quote: ... The integrity of the RNA was checked by capillary electrophoresis using the RNA 6000 Pico Labchip kit and the Bioanalyser 2100 (Agilent Technologies), and quantity was estimated using a Nanodrop 1000 (ThermoScientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... staggered QuikChange oligos AG66/AG67 were used to generate the A783U mutation in pTRE3G-HOTAIR using the QuikChange Site Directed Mutagenesis Kit (Agilent 200519) to generate pTRE3G-A783U_HOTAIR ...
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bioRxiv - Microbiology 2019Quote: ... A site directed mutagenesis of the cloned UBP-1 PCR product in the TOPO 2.1 vector was carried out using a QuikChange® multi-site directed mutagenesis kit (Agilent technologies) using the following primer combinations ...
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bioRxiv - Microbiology 2019Quote: ... Both infected and mock-infected RNA samples were labeled with Cy3 and Cy5 using an Agilent low-input RNA labeling kit (Agilent Technologies). Cy5-labeled infected or mock-infected samples were co-hybridized with Cy3-labeled mock-infected or infected samples in one array ...