Labshake search
Citations for Agilent :
7301 - 7350 of 9205 citations for Mouse Autophagy related protein 16 1 ATG16L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Genetics 2023Quote: ... Point mutations in KCNJ1 were introduced into the resulting pRS415TEF1-ROMK1 vector using either two-step overlap extension mutagenesis (141) or site-directed mutagenesis with the QuikChange kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutants of the vectors were constructed by site-directed mutagenesis using a QuikChange Site-Directed Mutagenesis kit (Stratagene, CA) (Yoshida et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... according to manufacturer’s instructions and the quality was assessed with the Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent Technologies). PolyA-enriched RNA libraries were prepared with the KAPA Stranded mRNA Sample Preparation Kit (Kapa Biosystems).
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bioRxiv - Cancer Biology 2023Quote: ... The mutagenic primers were designed using QuickChange primer design program at www.agilent.com/genomics/qcpd according to the guidelines mentioned in the manufacturer’s protocol (QuickChange Lightning Site-Directed Mutagenesis kit, Cat# 210518, Agilent Technologies). This was followed by mutant strand synthesis and transformation was performed as mentioned in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 50 μM 2-deoxy-glucose (2-DG) + 1μg/μL Hoechst (Seahorse XF Glycolysis Stress Test Kit, Agilent, 103020) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The concentration and quality of the synthesized MPXV mRNA were measured using an Agilent 2100 Bioanalyzer and RNA Nano 6000 Assay Kit (Agilent), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... DNA integrity was evaluated with a Fragment Analyzer (Advanced Analytical Technologies) using the HS NGS Fragment Kit (DNF-467, Agilent). dsDNA HS assay (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... UHOR_02675 active site mutants (Ribo1-ME80Q/D98N) were created from by using QuickChange XL Site-Directed Mutagenesis Kit from Agilent Technology according to manufactures instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and run on the Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit (Agilent Technologies, Santa Clara CA, USA) to confirm amplicon length and purity ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA concentration and quality of the RNAseq libraries were assessed using a 2100 Bioanalyzer with the High Sensitivity DNA Kit (Agilent), and libraries were sequenced as single-end 50 bp reads on an Illumina HiSeq 2500.
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected with either EGFP-AKT2 or EGFP-AKT2+ HA-SIRT5 constructs or left untransfected and after 48 hours were subjected to the Mitostress assay kit (Cat# 103015-100) from Agilent, USA28.
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bioRxiv - Neuroscience 2023Quote: ... Generation of GluK2 point mutants was performed by site-directed mutagenesis using the QuickChange Mutagenesis Kit (Stratagene, La Jolla, CA). Forward and reverse primer for creation of mutants were ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2 the XF Real-Time ATP Rate Assay Kit (Cat. No. 103592-100, Agilent Technologies, Cedar Creek, TX) was run following the manufacturer’s protocol using the Seahorse XF96 Analyzer as described above.
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bioRxiv - Microbiology 2023Quote: ... The quality and size distribution of the DNA was assessed on a Fragment Analyzer using the HS NGS Fragment Kit (Agilent). The pooled samples were then sequenced on a NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and the RNA integrity number (RIN) measured using the standard Agilent RNA 6000 Nano Kit Protocol and BioAnalyzer (Agilent Technologies). Extractions <7 RIN were not sequenced ...
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bioRxiv - Genetics 2023Quote: ... PREPLs was cloned into the bacterial expression vector pVP68K and mutations were introduced using the QuikChange II site directed mutagenesis kit (Stratagene) and confirmed by direct Sanger sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... The library size distribution was determined using an Agilent 2100 Bioanalyzer with the High Sensitivity DNA Kit (5067-4626, Agilent). We next used the SureSelect XT HS reagent Kits (5190-9685 and 5191-6686 ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cleaned up using the Takara Nucleospin kit per manufacturers protocol and concentration and size range assessed using the D5000 Screentape (Agilent). 200fmol of each sample was barcoded and subject to library prep using the Native Barcoding Ligation Kit with V14 chemistry (SQK-NBD-114-24 ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were validated using the NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified with the Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated by RNeasy Mini or RNeasy Plus Micro Kit and controlled for quality with a 2100 Bioanalyzer (Agilent) prior to cDNA libraries construction ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were tested for oxygen consumption rate and ATP production following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Measurements were taken both at baseline prior to mitochondrial stress and following the addition of 2 μM FCCP uncoupling agent.
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bioRxiv - Neuroscience 2023Quote: ... Transcribed cDNA was diluted ten-fold and used for real-time quantitative PCR (QuantiTect SYBR Green PCR Kit; Quiagen; 204145) performed with the MX3005P thermocycler (Stratagene).
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bioRxiv - Molecular Biology 2023Quote: ... mutant plasmids encoding the different RNA exosomopathy amino acid substitutions were generated by site-directed mutagenesis of the respective wild-type plasmids (pAC3656, pAC3652, pAC3482) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the desired missense mutations as previously described [33 ...
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bioRxiv - Genomics 2023Quote: ... the concentration and integrity of the RNA were assessed using the ND-1000 UV-visible light spectrophotometer from Nanodrop Technologies and the Bioanalyzer 2100 with the RNA 6000 Nano Lab Chip kit by Agilent.
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bioRxiv - Genetics 2023Quote: ... The quality and concentration of the library were assessed using the Femto Pulse Genomic DNA 165 kb Kit (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... The integrity of the RNA sample was verified using the Agilent RNA 6000 kit and Agilent Bioanalyzer 2100 (Agilent Technologies). Samples with an RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2023Quote: ... Specific nucleotide substitutions or coding-sequence truncations were introduced as required using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent). All plasmid inserts and modifications were authenticated by Sanger sequencing.
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bioRxiv - Genomics 2023Quote: ... and resulting size distribution was assessed with the High Sensitivity (HS) Large fragment kit run on the Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The integrity of the RNA was tested on a Bioanalyzer 2100 using the Prokaryote Total RNA Nano kit (Agilent Technologies) and RNA Integrity Numbers (RIN ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were analysed on the 2100 Bioanalyzer Agilent system using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626). DNA concentrations were measured by Qubit Quant IT kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Purified pools were quantified using Qubit dsDNA HS Assay and the quality checked using a Tapestation High Sensitivity D1000 kit (Agilent) to ensure the size distribution of amplicons was as expected ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was quantified by NanoDrop and loaded abundance measured using a Pico 6000 kit and Eukaryotic Total RNA assay on a BioAnalyzer (Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... The size distribution and quality of the libraries were evaluated using a high sensitivity DNA kit (Agilent Technologies #5067-4626). Quantification of libraries was carried out with the Quant-iT DNA Assay Kit ...
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bioRxiv - Cell Biology 2023Quote: ... the codon for C34 in pCEP4-MFAP4 was modified from TGC to TCG using the QuickChange Site Directed Mutagenesis kit as instructed by the manufacturer (Agilent). The entire insert in pCEP4-MFAP4 and the sequence coding for the C34S mutation in pCEP4- MFAP4C34S was validated by Sanger DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... The mutations corresponding to the MARylation sites of RACK1 (D144N/E145Q/D203N) were introduced into the pCMV3-RACK1 plasmid using the QuikChange Site-Directed Mutagenesis kit (Agilent).
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bioRxiv - Bioengineering 2023Quote: ... The library of CjCas9 variants was produced by random mutagenesis of the wild-type sequence using error-prone PCR approach (GeneMorph II kit from Agilent) (for primers ...
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bioRxiv - Biochemistry 2023Quote: ... The NopD point mutants constructs were generated by different primers and were created by the QuickChange site-directed mutagenesis kit (Stratagene). All primers are shown in the Supplementary Table1 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) and assessed on a BioAnalyzer 2100 (Agilent) for library quantification and quality control ...
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bioRxiv - Genomics 2023Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and post library QC was performed using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed as expected ...
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bioRxiv - Bioengineering 2023Quote: ... Quality assessment of the isolated RNAs from each condition was conducted using the RNA 6000 Pico Kit and the 2100 Bioanalyzer Systems (Agilent), while quantification was carried out using the Qubit system (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... vector containing the SARS-CoV-2 HA-ORF3a gene was used in site-directed mutagenesis using a QuikChange II site-directed mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... and quality checked using the Qubit dsDNA HS Assay Kit (Thermo cat. #Q32854) and a Bioanalyzer 2100 (Agilent cat. # G2939A) High Sensitivity DNA Kit (Agilent cat ...
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bioRxiv - Immunology 2024Quote: ... From this construct, CDC42 point mutants (R186C, C188Y, Y64C) were obtained by site-directed mutagenesis (Quick change kit, Agilent Technologies). The *192C*24 plasmid was produced by ThermoFisher.
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bioRxiv - Genomics 2024Quote: ... cDNA libraries were quantified using the Qubit dsDNA Assay Kit (Thermo, Q32851) and library quality was assessed with the 4150 Tapestation System (Agilent) and D5000 screen tapes (Agilent ...
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bioRxiv - Genetics 2024Quote: ... The BP-lowering rs1173771 haplotype was then constructed by site directed mutagenesis at the 11 SNPs locations in the haplotype using QuikChange II Site-directed mutagenesis kit (Agilent) and confirmed by Sanger sequencing ...
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bioRxiv - Immunology 2024Quote: ... The glycolytic function of infected macrophage was analyzed by the Agilent Seahorse XF Glycolytic Stress Test Kit (Agilent, 103020-100). Mφs and amastigotes were distributed in Seahorse XF96 tissue culture microplates for 3 days at 34°C ...
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bioRxiv - Biochemistry 2024Quote: The plasmid for expression of the rare codon reporter pCIneo-RLuc (30xRC) was generated by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis kit (Stratagene). Protein mutant plasmids used in this study are listed in Supplementary Table S1 ...
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bioRxiv - Genomics 2024Quote: ... RNA integrity (RIN) values for each sample were determined using the Agilent RNA 6000 Nano chip kit (Agilent, 5067-1511) and an Agilent Bioanalyzer 2100 instrument following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: ... and the mean fragment size was assessed using the DNA High Sensitivity KIT on a 2100 Bioanalyzer (Agilent Technologies, Netherlands). 4 nM pooled libraries was sequenced on NovaSeq S1 flow cell by Livestock Improvement Corporation Ltd ...