Labshake search
Citations for Agilent :
7201 - 7250 of 9205 citations for Mouse Autophagy related protein 16 1 ATG16L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Point mutations were introduced into Stx1A or Stx2 using a TA cloning vector with the QuickChange Site-Directed Mutagenesis Kit (Stratagene). Chimeric constructs of both syntaxin isoforms were obtained using Gibson Assembly (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... Mitochondrial energetics was measured using a Seahorse XFe96 extracellular flux analyzer and the XF Cell Mito Stress kit (Seahorse Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1zeo+/ nFlag-cmScarlet-hRobo1 was generated by inserting the Flag tag sequence at the N-terminus after the signal peptide sequence of Robo1 with QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Developmental Biology 2024Quote: The quality of the cDNA and resulting sequencing libraries were checked by bioanalyzer (High Sensitivity DNA Kit, Agilent, 5067-4626). The libraries were sequenced using Illumina NovaSeq PE150 ...
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bioRxiv - Genetics 2024Quote: Membranes were hybridized with “probe 4” for the HIS4::LEU2 hotspot 100 which was 32P-dCTP labelled using the Prime-It II Random Primer Labeling Kit (Stratagene). Following hybridization ...
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bioRxiv - Genetics 2024Quote: ... The SCN5A mutations E171Q (Ensembl transcript ENST00000333535 c.511G>C) and R222Q (c.665G>A) were introduced with a QuikChange Multi Lightning kit (Agilent) with primers 5’ttcaccgccatttacacctttCagtctctggtca (E171Q ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids expressing the BA.5 spike with the A570S mutation or the A570S/K147E double mutation were obtained by site-directed mutagenesis using a QuikChange II Site-Directed Mutagenesis Kit (Agilent). The dual split protein (DSP ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial stress test assay for pDC on the XF-HS Mini was optimized in-house within the ranges provided by the manufacturer and performed using the mitochondrial stress test kit (Agilent), with the addition of 2-DG (Millipore Sigma ...
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bioRxiv - Immunology 2024Quote: ... and RNA integrity was assessed with Agilent RNA 6000 nano kit (Aligent, Cat. No. 5067-1511) using an Agilent 2100 bioanalyzer (Agilent). The average A260/280 ratio was 1.9 (range 1.8-2.1) ...
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bioRxiv - Immunology 2024Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). ii ...
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bioRxiv - Systems Biology 2023Quote: Oxygen consumption rate was measured with the Seahorse XFe96 bioanalyzer using the Seahorse XF Cell Mito Stress Test Kit (Agilent), or using High-Resolution Respirometry (Oxygraph-2K ...
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bioRxiv - Neuroscience 2023Quote: ... ChIP-seq and RNA-seq library fragment size were measured by using high sensitivity DNA analysis kit (5067-4626, Agilent) on a TapeStation system (2200 ...
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bioRxiv - Immunology 2023Quote: ... An aliquot of the chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (Agilent) on Fragment Analyzer (Agilent) ...
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bioRxiv - Genomics 2023Quote: RNA quality was determined initially by nanodrop (A260/A280 and A260/A230 ratios) and then via Agilent Fragment Analyzer 5200 using the RNA (15nt) DNF-471 kit (Agilent). All samples achieved Nanodrop ratios of >1.8 and Fragment Analyzer RIN >9.0 prior to sequencing and indicated high quality RNA ...
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bioRxiv - Neuroscience 2023Quote: ... Each library was quantified by Qubit dsDNA BR or HS Assay kits and assessed by TapeStation using D1000 or HS D1000 DNA tapes (Agilent). was determined using Qubit dsDNA HS Assay Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Integrity was assessed using Agilent RNA 6000 Pico Kit on a 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA, USA). RNA sequencing libraries were prepared using the Illumina Stranded mRNA prep ligation kit (Illumina ...
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bioRxiv - Biophysics 2024Quote: ... The pBAD30-ClsA (EE) G51A mutant plasmid was generated from the pBAD30- ClsA (EE) template with QuikChange II Site-Directed Mutagenesis Kit (Agilent). Two residues ...
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bioRxiv - Biochemistry 2024Quote: All of the mutations were introduced into a plasmid backbone expressing 6xHis tagged pNL4-3-derived IN by QuikChange site-directed mutagenesis kit (Agilent). The plasmids containing full-length WT and mutants were transformed into BL21 (DE3 ...
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bioRxiv - Cancer Biology 2024Quote: ... The library average fragment size and quantification was performed using Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Cat. 5067-4626) and a final concentration determination was performed using NEBNext® Library Quant Kit for Illumina (New England Biolabs Cat ...
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bioRxiv - Cell Biology 2023Quote: ... labeled cRNA was prepared from 0.1 micro-g Total RNA using the Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... An aliquot of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment AnalyzerTM (Agilent). ChIP was performed using IP-Star® Compact Automated System (Diagenode Cat# B03000002 ...
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bioRxiv - Biochemistry 2023Quote: RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-8000 (Nanodrop Technologies) and RNA integrity and concentration were assessed using a Fragment Analyzer SS-RNA kit (Agilent). Per sample ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: XF Cell Mito and XF Glycolysis Stress Test Kits were used to measure mitochondrial and glycolytic function on a Seahorse Extracellular Flux Analyzer (Agilent). The Seahorse microplate was coated with Cultrex (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... and their quality was assessed by capillary electrophoresis on the FragmentAnalyzer using the NGS High Sensitivity kit (DNF-474, Agilent). The libraries were pooled and sequenced on an Illumina NovaSeq 2000 ...
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bioRxiv - Microbiology 2023Quote: ... and a barcoding primer (unique for each sample to allow for demultiplexing of reads) with the Easy A cloning kit (Agilent). A 200–400 bp product was gel-purified and sequenced on an Illumina NextSeq Platform (in lab ...
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bioRxiv - Microbiology 2023Quote: ... This DNA was then subject to an initial round of PCR amplification with primers oTB535 and oTB536 with the Easy A cloning kit (Agilent), digested with NotI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfer of the Gateway cassette from these two entry vectors to the pBIFCt-2in1-NN destination vector 57 was performed through LR clonase II kit (Agilent).
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bioRxiv - Biochemistry 2022Quote: ... The quality of total RNA was examined using a fragment analyzer system with an Agilent HS RNA kit (Agilent Technologies). Libraries were prepared using an MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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bioRxiv - Microbiology 2023Quote: ... and pKTP109 (catR, Para::ftsI) by using the QuickChange site-directed mutagenesis kit according to the manufacturer’s instruction (Agilent Technologies). The list of primers used in this study was previously published.
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bioRxiv - Cancer Biology 2023Quote: The metabolic profile of 1205Lu melanoma cells was assessed using the Seahorse XF Cell Energy Phenotype Test Kit (Cat no.103325-100, Agilent). Both parental and NKX2.2 KO 1205Lu cells ...
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bioRxiv - Cell Biology 2023Quote: ... The tau P301L mutants were generated by PCR using QuickChange Lightning Site-Direct mutagenesis kit (Agilent Technologies, Santa Clara, USA). For all intermolecular sensors ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... and RNA Integrity Number (RIN) was assessed using Agilent RNA 6000 Pico Kit and an Agilent 2100 bioanalyzer (Agilent, USA). Library preparation and sequencing was performed by BGI (Shenzhen ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent, G2964AA) with RNA ScreenTape (Agilent ...
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bioRxiv - Pathology 2023Quote: ... RNA Integrity Number (RIN) and DV200 were determined by BioAnalyzer electrophoresis using an RNA 6000 Pico Kit (#5067-1513, Agilent). The DV200 metric refers to the percentage of total profiled RNA fragments greater than 200bp in length ...
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bioRxiv - Systems Biology 2022Quote: ... Adherent NSCs were infected with retro-Cre-Tomato retrovirus (5μl of virus was used to infect 3x105 cells) or nucleofected with expression constructs according to instructions of the Amaxa nucleofection kit (Agilent) [28] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Amplified and purified sequencing libraries were analyzed on an Agilent 2100 Bioanalyzer (High sensitivity DNA analysis kit, Agilent, 5067-4626), pooled in equimolar amounts (2.47 ng/μL) ...
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bioRxiv - Molecular Biology 2022Quote: ... Point mutations in the repM gene were generated using specific primers and a QuikChange Site-Directed Mutagenesis kit according to the protocol supplied by the manufacturer (Stratagene). All oligonucleotide primers used in this study are listed in Supplementary Table S2.
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bioRxiv - Cancer Biology 2023Quote: ... The stranded mRNA libraries were analyzed on a 2100 Bioanalyzer instrument following the manufacturer’s protocol “Agilent DNA 7500 kit” (G2938-90024, Agilent Technologies), diluted to 10nM and pooled equimolar into multiplex sequencing pools for paired end sequencing on the NovaSeq 6000 Illumina sequencing instrument ...
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bioRxiv - Immunology 2023Quote: ... All mutations in this study were introduced using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Cat #200521), KLD Enzyme Mix ...
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bioRxiv - Immunology 2022Quote: ... SARS-COV-2-Strunc variants were generated in house by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) starting from synthetic DNA (Genscript) ...
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bioRxiv - Molecular Biology 2023Quote: ... and C66A triple mutation), KR5>A (contains K286A, R287A, K288A, K300A, and R301A mutations) were done using QuikChange Mutagenesis Kit (Agilent). Deletion mutants (1-280 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting pHD1344tub(PAC)-mt-LAF3-Cterm3V5 plasmid was then used as a template for site-directed mutagenesis of D211 (QuikChange II kit; Agilent) using forward and reverse primers listed in Supplemental Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Genetics 2023Quote: ... after which DNA fragments in a range of 400-700 bp were excised and extracted using Gel Extraction Kit (A&A Biotechnology) The quality and quantity of the libraries were verified with TapeStation system (Agilent) and Qubit 2.0 fluorometer ...
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bioRxiv - Developmental Biology 2023Quote: ... Quality of the final library preparation was analysed on the Bioanalyzer using the High Sensitivity DNA reagents kit and cassettes (5067-4626, Agilent). For all the steps involving purification of library intermediates ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted using the ZYMO RNA purification kit and RNA integrity was confirmed by the TapeStation system (Agilent). Total RNA was used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... all libraries were ligated to NEBNext® Multiplex Oligos for Illumina® (Dual Index Primer Set I) and quality control was performed using a 2100 Bioanalyser High Sensitivity DNA Kit 5067-4626 (Agilent) and libraries quantified using a QubitTM Fluorometer following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... we created all other Gαi3 mutants featured in this work using a QuikChange® Site-Directed Mutagenesis Kit as per manufacturer’s protocol (Stratagene, USA). A variant that is insensitive to ADP-ribosylation by Pertussis Bordetella toxin (PTX ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...