Labshake search
Citations for Agilent :
6351 - 6400 of 6919 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were quality controlled using a 2100 Bioanalyzer system with Agilent High Sensitivity DNA Kit (Agilent Technologies, Inc., 5067-4626) and quantified with Qubit™ 1X dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The quality of sequencing libraries was validated with a Bioanalyzer 2100 using the Agilent High Sensitivity DNA Kit (Agilent, 5067-4626). The pooled sequencing libraries were quantified and sequenced on either the MiSeq (v2 or v2 nano ...
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bioRxiv - Biophysics 2022Quote: ... Amber codon mutagenesis in the selected positions were performed using the QuickChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, California, USA) following the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: PTEN monoubiquitination mutants PTEN-K13R and PTEN-K289R were generated from GFP-PTEN by site directed mutagenesis using QuickChange XL Site Directed Mutagenesis Kit (Agilent technologies). Table S1 includes the primer sequences.
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bioRxiv - Genomics 2022Quote: ... Quality check and quantification of total RNA were performed using the Agilent Bioanalyzer 2100 in combination with the RNA 6000 Nano Kit (Agilent Technologies). Libraries were constructed from 500 ng total RNA using NEBNext Ultra II RNA - polyA+ (mRNA ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... and the concentration and RNA Integrity Number (RIN) was determined with a RNA 6000 nano kit on 2100 Bioanalyzer instrument (Agilent Technologies). Real-time PCR was performed on samples in duplicate on an ABI 7900HT Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2023Quote: ... The final libraries were then quality checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent, 5067-4626) and Qubit dsDNA HS Assay kit (Thermo Scientific ...
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bioRxiv - Genetics 2023Quote: ... Transcriptional profiling was performed on mouse colon samples using the SurePrint G3 Mouse GE8×60K Microarray kit (design ID:028005, Agilent Technologies). Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies) ...
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bioRxiv - Genetics 2023Quote: ... Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies). The specific activities and cRNA yields were determined by using a NanoDrop ND-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... Three Myc epitopes were then inserted before the stop codon into the three plasmids with the QuickChange Site-Directed Mutagenesis Kit (Agilent Technologies) to get SLITRK3-WT-Myc ...
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bioRxiv - Cancer Biology 2023Quote: ... and RT-qPCR was performed using the 1-Step Brilliant II SYBR Green quantitative RT-PCR master mix kit (Agilent Technologies) on a Bio-Rad C1000 Thermal Cycler CFX96 Real-Time System ...
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bioRxiv - Cancer Biology 2022Quote: ... were constructed from pcDNA3.1+-C-(K)-DYK-EBP plasmids using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, #210519) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA libraries were constructed with the NEBNext® Ultra™ II FS DNA Library Prep Kit (Agilent, CA, US). Thereafter ...
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bioRxiv - Physiology 2023Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the fusion constructs were made from the appropriate backbone vector with the QuikChange Lightning Multi Site Directed Mutagenesis kit (Agilent Technologies). Reporters were sequenced from Genewiz before transfecting the parasites ...
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bioRxiv - Microbiology 2023Quote: ... AIP56D274S/D276-278S and AIP56D274N/D276-278N with or without V5-tag were generated with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, 200518) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and library profiles were assessed using a High Sensitivity DNA kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Libraries were then sequenced on an Illumina NovaSeq instrument (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... The final library was assessed for quality using the Agilent TapeStation System with a D1000 kit (Agilent Technologies, Santa Clara, California), and paired-end ...
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bioRxiv - Cell Biology 2023Quote: The NR2A(M705V) or NR2A(A727T) mutation was introduced to the NR2A subunit using QuikChange II site-directed mutagenesis Kit (Agilent Genomics). DNA sequencing was used to confirm the cDNA sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... The quality and quantity of libraries were determined using Fragment Analyzer by DNF-474 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies) and QuantiFluor dsDNA System (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was quantified and quality checked using Bioanalyzer 2100 instrument in combination with RNA 6000 nano kit (both Agilent Technologies). Libraries were prepared from 800 ng of input material (total RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The above residue mutants were created by site-directed mutagenesis following instructions provided in the QuickChange XL Site-Directed Mutagenesis Kit manual (Agilent Technologies). All other full-length UNK residue mutants ...
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bioRxiv - Microbiology 2023Quote: ... into AAATA in the DENV-2 wild type plasmid (pFK-DVs) was generated using QuickChange XL site-directed mutagenesis kit (Agilent, 200517) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA integrity was determined using capillary chip-based electrophoresis with an RNA 6000 Nano Methods 59 LabChip Kit and an Agilent Bioanalyzer 2100 (Agilent, USA) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... GGC -> GGT) of dCas9 on pKR387 to remove an AscI recognition site using the QuikChange II site-directed mutagenesis kit (Agilent #200523). The resulting product was transformed into NEB 10-beta cells using a standard heat shock protocol and transformants were selected on LB agar plates with 100 μg/mL of carbenicillin ...
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bioRxiv - Neuroscience 2024Quote: RNA from sorted cells were analyzed on the Agilent 2100 Bioanalyser system using the Eukaryote Total RNA Pico kit (Agilent Technologies) and the mean RIN number was 9.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Twin-strep-Flag-HALO-DVL3 or pCS2+xDvl3 vector was performed using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) following a manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of RNA samples was used for reverse transcription with the AccuScript High-Fidelity 1st Strand cDNA Synthesis Kit (Agilent; 200820) using random hexamer primers ...
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bioRxiv - Microbiology 2023Quote: ... The purified libraries were then assessed for their fragment sizes using the High Sensitivity DNA kit on the Bioanalyzer 2100 (Agilent Technologies). After applying the NetoVIR protocol ...
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bioRxiv - Immunology 2023Quote: ... The size of the resulting libraries was determined with the Agilent D1000 reagent kit and Agilent 4200 TapeStation (Agilent Technologies, USA). Pooling was performed automatically using a Tecan Freedom EVO robotic station (Tecan ...
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bioRxiv - Immunology 2023Quote: ... and quality was verified on an Agilent 2100 Bioanalyzer instrument with the Agilent DNA 1000 kit (Agilent Technologies, Catalog# 5067-1504). Additionally ...
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bioRxiv - Molecular Biology 2023Quote: ... The desired PNUTS mutant in the RVXF motif (converting the RISW motif to RASA) was generated by oligonucleotide-mediated site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell RNA-seq and CMO libraries were prepared according to the manufacturer’s protocol and the library quality was confirmed with a Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). Cellecta barcode libraries were prepared using 25% of the adaptor-ligated cDNA to amplify only the barcoded cDNA product ...
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bioRxiv - Biochemistry 2023Quote: Mutations were generated by site-directed mutagenesis (table 2) on this plasmid using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and plasmids were verified through sequencing through the Molecular Informatics Core at the University of Rhode Island.
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bioRxiv - Biochemistry 2023Quote: ... The desired PP1 or PNUTS mutants were generated by oligonucleotide- mediated site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... H80S/D83S and G93A mutants were generated by the use of QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) (60) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... as previously described.19 Site-directed mutagenesis was performed using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Protease activity of NIa-Pro was abolished by mutating cysteine 151 to alanine (C151A) using the same construct and the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA) in both pMDC32 and pSITE (Huogen et al. ...
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bioRxiv - Biophysics 2023Quote: ... site-directed mutagenesis was used using the vector pET21a-αSynuclein with the kit QuikChange XL Site-Directed Mutagenesis (Agilent technologies, USA) and the inserted mutations were checked by sequencing ...
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bioRxiv - Immunology 2023Quote: ... RNA quality was verified using the Fragment Analyzer (Advanced Analytical) and the RNA kit (DNF-471, Agilent Technologies, Wilmington, DE, USA). All samples with an RNA Quality Number (RQN ...
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was checked with an RNA Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Site-specific mutagenesis was performed according to standard procedures of the QuickChange Site-Directed Mutagenesis Kit purchased from Stratagene (GE Healthcare) or Mut Express II (Vazyme Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... Quality control checks and quantification were performed using an Agilent 2100 Electrophoresis Bioanalyzer instrument with the Agilent RNA 6000 Pico kit (Agilent Technologies), and a Qubit 2.0 fluorometer with the Qubit RNA HS Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: Site-directed mutagenesis of SARS-CoV-2 spike was performed with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent 200522), using primers listed in Supplementary table S2 and according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... To assess quality and size of the libraries samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies). Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified total RNA samples (100 ng) were labeled with Cyanine3 (Cy3) using a Low Input Quick Amp WT labeling kit (One-color; Agilent Technologies). The Cy3-labeled cRNA samples were checked for amount (>825 ng ...
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bioRxiv - Developmental Biology 2024Quote: ... HySp5-2992-mBS4:Luc and HySp5-2992-mBS5:Luc constructs were generated using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Genomics 2024Quote: ... RNA samples were quantified and RNA integrity numbers (RIN) derived using the Bioanalyzer High Sensitivity RNA 6000 Pico kit (Agilent, UK).
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bioRxiv - Genomics 2024Quote: ... and the RNA integrity number (RIN) was measured using a tape-station system with an RNA screen-tape analysis kit (Agilent Technologies).