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Citations for Agilent :
6201 - 6250 of 6919 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Site-directed PCR mutagenesis was then carried out to mutate the codon corresponding to valine 189 (GTC into GAC) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). Overlapping PCR was used to add a C-terminus 3Flag epitope and a marker conferring resistance to Nourseothricin and the resulting PCR fragment was transformed into fission yeast using routine protocols ...
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bioRxiv - Microbiology 2019Quote: ... encoding the I78A variant of alt-SDHC was obtained by site-directed mutagenesis using QuikChange® II Site Directed Mutagenesis kit (Stratagene) following provider’s instructions and oligos listed in S3 dataset ...
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bioRxiv - Immunology 2019Quote: ... cDNA was subjected to quality control using 1 µl of amplified cDNA on an Agilent 2100 BioAnalyser and Agilent High Sensitivity DNA kits (Agilent Technologies). 5 µl of cDNA per cell was cleaned using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... RT qPCR analysis was run for the samples using a miScript SYBR Green PCR Kit (Cat. No. 218073) on a Stratagene Mx3005P (Agilent Technologies). Housekeeping gene GAPDH primers were purchased from Qiagen (Cat ...
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bioRxiv - Genetics 2021Quote: RNA-sequencing libraries were prepared using the SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing kit (Agilent, Cat#: G9691A) according to the manufacturer’s protocol using 100 ng total RNA ...
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bioRxiv - Cell Biology 2021Quote: ... The mutant eGFP-CCR5-L196K (substitution of L196 with a lysine) was generated by site-directed mutagenesis using the QuickChange II Mutageneis kit (Agilent Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Disruption of the Lmxb1 binding sites was performed using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) following manufacturer recommendations and confirmed by Sanger sequencing.
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bioRxiv - Cell Biology 2020Quote: ... HA-USP29 siRNA-resistant and the catalytically inactive USP29C294S were generated using the QuikChange® II XL Site-Directed Mutagenesis Kit (Stratagene) and the oligos reported in Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... amino acid substitutions were introduced using a QuikChange II XL site directed mutagenesis kit (200521) purchased from Agilent (Santa Clara, CA). After transformation into BL21-Gold (DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... The quality of obtained libraries were analyzed using Agilent Bioanalyzer 2100 and High Sensitivity DNA kit (Agilent, cat. no. 5067-4626). Finally ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... Total RNA from all other cell lines was used to prepare cDNA sequencing libraries using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies) after the polyA selection step ...
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bioRxiv - Cell Biology 2019Quote: ... and integrated lentiviral sgRNA sequences were amplified by a two-step PCR reaction (20 cycles and 14 cycles, respectively) as previously described (32, 41) using a Herculase II Fusion DNA Polymerase kit (Agilent biotechnologies). DNA was purified after each of the PCR reactions using a QIAquick PCR purification kit (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration and quality of the purified libraries were determined using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626) on the Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: The EnVision™ staining kit (G|2 Double-stain System, Rabbit/Mouse, DAB+/Permanent RED code K5361; Agilent technologies, Dako DK) was used for the immunohistochemical stain of Mamu-DR ...
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bioRxiv - Neuroscience 2021Quote: The EnVision™ staining kit (G|2 Double-stain System, Rabbit/Mouse, DAB+/Permanent RED code K5361; Agilent technologies, Dako DK) was used for the immunohistochemical stain of Mamu-DR ...
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bioRxiv - Biophysics 2021Quote: Mutations were introduced into the human CFTR(E1371S)/pGEMHE and CFTR(D1370N)/pGEMHE sequences using the QuikChange II XL Kit (Agilent Technologies) and confirmed by automated sequencing (LGC Genomics GmbH) ...
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bioRxiv - Biochemistry 2021Quote: ... Multi-site-directed mutagenesis was carried out using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). Proteins were expressed in and purified from BL21-CodonPlus(DE3)pLysS Escherichia coli (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... The quality and quantity of RNA was determined by capillary electrophoresis using HS RNA kit and 5200 Fragment Analyzer (Agilent Technologies). For single-end RNA sequencing (RNA-seq) ...
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bioRxiv - Genomics 2021Quote: ... site-directed mutagenesis was performed targeting desired codon positions of the rplD and rplV genes on the pS10 plasmid [20] using the QuikChange Lightning kit (Agilent Technologies) and the primers indicated in Supplemental Table 2 ...
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bioRxiv - Biophysics 2021Quote: ... the double-mutant S11N/G74S variant and the chimera involving the loop [70-77] were introduced using the QuikChange Lighting Site-Directed Mutagenesis kit (Agilent Technologies) and the sequences were confirmed by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... followed by library interrogation using a Bioanalyzer 2100 instrument with Agilent High Sensitivity DNA Kit (Agilent Technologies, Inc. Part# 5067-4626).
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Neuroscience 2021Quote: ... Cy3- or Cy5- labeled cRNA probe was synthesized from 150 ng of total RNA using the Quick-Amp Labeling Kit (Agilent Technologies). Quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-2000 spectrophotometer ...
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bioRxiv - Immunology 2020Quote: ... cDNA before and after TCR enrichment was profiled using both Qubit (Thermofischer scientific) and Bioanalyzer High Sensitivity DNA kit (Agilent Technologies). Libraries for RNA-seq and V(D)J were prepared following the manufacturer’s user guide (10X Genomics) ...
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bioRxiv - Molecular Biology 2021Quote: ... Enzymatically-dead zDHHC3 mutant transgenic mice overexpressing zDHHC3DHHS in cardiomyocytes were generated by site-directed mutagenesis of the α-MHC-Zdhhc3 promoter-transgene construct using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) to encode a mutation of Cys-157 in mouse zDHHC3 protein to Ser ...
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bioRxiv - Molecular Biology 2020Quote: ... integrity and the amount of total RNA were measured with a ‘Bioanalyzer 2100’ using an ‘RNA Nano 6000 Microfluidics Kit’ (both Agilent genomics). RNA labeling was performed with the two color ‘Quick Amp Labeling Kit’ (Agilent genomics ...
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bioRxiv - Neuroscience 2021Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Microbiology 2021Quote: ... The MPER changes (L669S, W672A, I675A, T676A and L679A) were introduced using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). All the mutations were confirmed by DNA sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA extracted from FACS isolated cells was quantified and assessed for quality using the RNA 6000 Pico Kit (Agilent – 5067-1513) and an Agilent 2100 Bioanalyzer ...
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bioRxiv - Developmental Biology 2020Quote: ... Post cDNA amplification and post library construction quality control was performed using the Agilent Bioanalyzer High Sensitivity kit (Agilent 5067-4626). Libraries were sequenced using a NovaSeq 6000 and the S2 flow cell ...
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bioRxiv - Cell Biology 2021Quote: ... and -ΔPID (aa 524-571) domain deletions were generated by PCR and the R57D mutant by the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and quality was determined with a DNA ScreenTape Kit on the TapeStation 2200 system (Agilent, 5067-5582, 5067-5583, 5067-5586). Paired end sequencing was done using a HiSeq 2500 (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: Whole RNA was extracted from three replicates for each of the cell-lines followed by a quality assessment using Agilent Bioanalyser-2100 with Agilent nano Kit (Agilent Technologies). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... and the quality and quantity of ds-cDNA was determined with NanoDrop 1000 and a 2100 Bioanalyzer using the Agilent DNA 7500 kit (Agilent Technologies). Four hundred ng of total RNA yielded on average 12 μg of ds-cDNA ...
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bioRxiv - Cancer Biology 2022Quote: ... and a p72Plk3 point mutant (Plk3R354G) was generated via site-directed mutagenesis using a QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies). All mutants were subcloned into the pCMV-Tag2A vector ...
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bioRxiv - Cell Biology 2022Quote: ... ASNA-1A63V::GFP was generated by mutagenesis of pVB222GK (Kao et al., 2007) to introduce the A63V change using the QuickChange II Kit (Agilent Technologies). The resulting plasmid (pGK200 ...
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bioRxiv - Microbiology 2022Quote: ... ΔNef ANCH3 (HIV-1 ANCH3 IND116A) was obtained by insertional mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent #200522), for integrase (IN ...
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bioRxiv - Molecular Biology 2022Quote: ... The quality of the prepared libraries was assessed using an Agilent 2100 bioanalyzer and a High Sensitivity DNA kit (Agilent Technologies), or Fragment Analyzer and dsDNA 915 Reagent Kit (Agilent Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... Point mutations (I91F and K176E) were introduced using the QuickChange II XL Site-Directed Mutagenesis Kit (SI Table 1; Agilent Technologies). The full-length sequence of the PAI-1 constructs was confirmed by Sanger sequencing (SI Table 1) ...
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bioRxiv - Biophysics 2022Quote: ... point mutations were introduced to cDNA by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Inc.) and PrimeSTAR Max DNA Polymerase (Takara Bio Inc. ...
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bioRxiv - Genetics 2022Quote: To introduce the T1035S or R1147W mutations we used the QuikChange II XL Multi Site-Directed Mutagenesis kit (Agilent Technologies, #200521). The primers for the single mutagenesis experiments were designed by QuikChange software (Stratagene ...
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bioRxiv - Biophysics 2022Quote: ... PPARγ2 mutants were generated by nucleotide substitution using the QuikChange II XL Site Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... c-Src cloned in pBluescript SK+ vector through BamHI and XbaI sites was mutated using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) with the forward primer (5’-CCACTTTCGTGGCTCTCGAGGACTACGAGTCCCGGACTG-3’ ...
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bioRxiv - Cell Biology 2022Quote: Glycolysis stress kit was used to study acute ECAR change upon TNFα stimulation using a seahorse eXF96 extracellular flux analyzer (Seahorse Bioscience). HLMVECs were seeded in an XF96 cell plates at 25,000 cells/well for analysis the following day ...
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bioRxiv - Cell Biology 2022Quote: ... All cell lines tested negative for mycoplasma using the Mycoplasma Plus™ PCR kit (#302008, Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Genomics 2022Quote: ... The final size of ∼250 bp was assessed using a Fragment Analyzer genomic DNA analysis Kit (Agilent Technologies, Santa Clara, CA). For library preparation ...
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bioRxiv - Immunology 2022Quote: ... the cDNA was evaluated for size distribution and quantity using the Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies) and the Qubit dsDNA HS Assay Kit (ThermoFisher ...
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bioRxiv - Genetics 2022Quote: ... 2014) bearing FRQ point mutations to restriction-digested pCB05 in place of the QuickChange II® Site-directed Mutagenesis Kit (Stratagene). Four primer sets were used as flanks to facilitate homologous recombination in a yeast strain (FY834 ...