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Citations for Agilent :
6201 - 6250 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Mitochondrial energetics was measured using a Seahorse XFe96 extracellular flux analyzer and the XF Cell Mito Stress kit (Seahorse Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1zeo+/ nFlag-cmScarlet-hRobo1 was generated by inserting the Flag tag sequence at the N-terminus after the signal peptide sequence of Robo1 with QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Genetics 2024Quote: Membranes were hybridized with “probe 4” for the HIS4::LEU2 hotspot 100 which was 32P-dCTP labelled using the Prime-It II Random Primer Labeling Kit (Stratagene). Following hybridization ...
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bioRxiv - Developmental Biology 2024Quote: The quality of the cDNA and resulting sequencing libraries were checked by bioanalyzer (High Sensitivity DNA Kit, Agilent, 5067-4626). The libraries were sequenced using Illumina NovaSeq PE150 ...
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bioRxiv - Genetics 2024Quote: ... The SCN5A mutations E171Q (Ensembl transcript ENST00000333535 c.511G>C) and R222Q (c.665G>A) were introduced with a QuikChange Multi Lightning kit (Agilent) with primers 5’ttcaccgccatttacacctttCagtctctggtca (E171Q ...
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bioRxiv - Immunology 2024Quote: ... and RNA integrity was assessed with Agilent RNA 6000 nano kit (Aligent, Cat. No. 5067-1511) using an Agilent 2100 bioanalyzer (Agilent). The average A260/280 ratio was 1.9 (range 1.8-2.1) ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids expressing the BA.5 spike with the A570S mutation or the A570S/K147E double mutation were obtained by site-directed mutagenesis using a QuikChange II Site-Directed Mutagenesis Kit (Agilent). The dual split protein (DSP ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial stress test assay for pDC on the XF-HS Mini was optimized in-house within the ranges provided by the manufacturer and performed using the mitochondrial stress test kit (Agilent), with the addition of 2-DG (Millipore Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and quantity of both total and PolyA+ RNA samples were assessed using the Agilent 2100 Bioanalyzer with the RNA 6000 Nano LabChip Kit (Agilent), the NanoDrop 1000 Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). ii ...
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bioRxiv - Neuroscience 2023Quote: Point mutations were introduced into Stx1A or Stx2 using a TA cloning vector with the QuickChange Site-Directed Mutagenesis Kit (Stratagene). Chimeric constructs of both syntaxin isoforms were obtained using Gibson Assembly (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were analysed on the 2100 Bioanalyzer Agilent system using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626). DNA concentrations were measured by Qubit Quant IT kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... the codon for C34 in pCEP4-MFAP4 was modified from TGC to TCG using the QuickChange Site Directed Mutagenesis kit as instructed by the manufacturer (Agilent). The entire insert in pCEP4-MFAP4 and the sequence coding for the C34S mutation in pCEP4- MFAP4C34S was validated by Sanger DNA sequencing ...
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bioRxiv - Genetics 2023Quote: ... The quality and concentration of the library were assessed using the Femto Pulse Genomic DNA 165 kb Kit (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... the concentration and integrity of the RNA were assessed using the ND-1000 UV-visible light spectrophotometer from Nanodrop Technologies and the Bioanalyzer 2100 with the RNA 6000 Nano Lab Chip kit by Agilent.
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bioRxiv - Cell Biology 2023Quote: ... The mutations corresponding to the MARylation sites of RACK1 (D144N/E145Q/D203N) were introduced into the pCMV3-RACK1 plasmid using the QuikChange Site-Directed Mutagenesis kit (Agilent).
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bioRxiv - Immunology 2023Quote: ... Specific nucleotide substitutions or coding-sequence truncations were introduced as required using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent). All plasmid inserts and modifications were authenticated by Sanger sequencing.
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bioRxiv - Genomics 2023Quote: ... The integrity of the RNA sample was verified using the Agilent RNA 6000 kit and Agilent Bioanalyzer 2100 (Agilent Technologies). Samples with an RNA Integrity Number (RIN ...
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bioRxiv - Genomics 2023Quote: ... Mean length and library concentration was determined by running libraries on a 2100 bioanalyzer using a DNA 1000 Kit (Agilent). RNA-seq libraries were sequenced by the Norwegian Sequencing Center (Oslo ...
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bioRxiv - Immunology 2024Quote: ... The resulting libraries tagged with unique dual indices were checked for size and quality using the Agilent High Sensitivity DNA Kit (Agilent). Library concentrations were measured using the KAPA SYBR Fast qPCR kit and loaded for sequencing on the Illumina NovaSeq 6000 instrument (paired-end 2X150 ...
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bioRxiv - Cancer Biology 2023Quote: The metabolic profile of 1205Lu melanoma cells was assessed using the Seahorse XF Cell Energy Phenotype Test Kit (Cat no.103325-100, Agilent). Both parental and NKX2.2 KO 1205Lu cells ...
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bioRxiv - Cell Biology 2023Quote: ... The tau P301L mutants were generated by PCR using QuickChange Lightning Site-Direct mutagenesis kit (Agilent Technologies, Santa Clara, USA). For all intermolecular sensors ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity of brain organoids and mouse tissue was evaluated by analysis on the 2100 Bioanalyzer RNA 6000 Pico Chip kit (Agilent) using the RNA Integrity Number (RIN) ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining visualisation was performed by incubating with a cocktail of anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Envision Kit, Agilent) for 60 minutes at room temperature ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... and RNA Integrity Number (RIN) was assessed using Agilent RNA 6000 Pico Kit and an Agilent 2100 bioanalyzer (Agilent, USA). Library preparation and sequencing was performed by BGI (Shenzhen ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent, G2964AA) with RNA ScreenTape (Agilent ...
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bioRxiv - Pathology 2023Quote: ... RNA Integrity Number (RIN) and DV200 were determined by BioAnalyzer electrophoresis using an RNA 6000 Pico Kit (#5067-1513, Agilent). The DV200 metric refers to the percentage of total profiled RNA fragments greater than 200bp in length ...
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bioRxiv - Systems Biology 2022Quote: ... Adherent NSCs were infected with retro-Cre-Tomato retrovirus (5μl of virus was used to infect 3x105 cells) or nucleofected with expression constructs according to instructions of the Amaxa nucleofection kit (Agilent) [28] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Amplified and purified sequencing libraries were analyzed on an Agilent 2100 Bioanalyzer (High sensitivity DNA analysis kit, Agilent, 5067-4626), pooled in equimolar amounts (2.47 ng/μL) ...
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bioRxiv - Molecular Biology 2022Quote: ... Point mutations in the repM gene were generated using specific primers and a QuikChange Site-Directed Mutagenesis kit according to the protocol supplied by the manufacturer (Stratagene). All oligonucleotide primers used in this study are listed in Supplementary Table S2.
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bioRxiv - Cancer Biology 2023Quote: ... The stranded mRNA libraries were analyzed on a 2100 Bioanalyzer instrument following the manufacturer’s protocol “Agilent DNA 7500 kit” (G2938-90024, Agilent Technologies), diluted to 10nM and pooled equimolar into multiplex sequencing pools for paired end sequencing on the NovaSeq 6000 Illumina sequencing instrument ...
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bioRxiv - Immunology 2023Quote: ... All mutations in this study were introduced using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Cat #200521), KLD Enzyme Mix ...
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bioRxiv - Immunology 2022Quote: ... SARS-COV-2-Strunc variants were generated in house by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) starting from synthetic DNA (Genscript) ...
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bioRxiv - Molecular Biology 2023Quote: ... and C66A triple mutation), KR5>A (contains K286A, R287A, K288A, K300A, and R301A mutations) were done using QuikChange Mutagenesis Kit (Agilent). Deletion mutants (1-280 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting pHD1344tub(PAC)-mt-LAF3-Cterm3V5 plasmid was then used as a template for site-directed mutagenesis of D211 (QuikChange II kit; Agilent) using forward and reverse primers listed in Supplemental Table 1 ...
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bioRxiv - Genomics 2023Quote: XF Cell Mito and XF Glycolysis Stress Test Kits were used to measure mitochondrial and glycolytic function on a Seahorse Extracellular Flux Analyzer (Agilent). The Seahorse microplate was coated with Cultrex (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... and their quality was assessed by capillary electrophoresis on the FragmentAnalyzer using the NGS High Sensitivity kit (DNF-474, Agilent). The libraries were pooled and sequenced on an Illumina NovaSeq 2000 ...
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bioRxiv - Microbiology 2023Quote: ... and a barcoding primer (unique for each sample to allow for demultiplexing of reads) with the Easy A cloning kit (Agilent). A 200–400 bp product was gel-purified and sequenced on an Illumina NextSeq Platform (in lab ...
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bioRxiv - Microbiology 2023Quote: ... This DNA was then subject to an initial round of PCR amplification with primers oTB535 and oTB536 with the Easy A cloning kit (Agilent), digested with NotI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfer of the Gateway cassette from these two entry vectors to the pBIFCt-2in1-NN destination vector 57 was performed through LR clonase II kit (Agilent).
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bioRxiv - Biochemistry 2022Quote: ... The quality of total RNA was examined using a fragment analyzer system with an Agilent HS RNA kit (Agilent Technologies). Libraries were prepared using an MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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bioRxiv - Microbiology 2023Quote: ... and pKTP109 (catR, Para::ftsI) by using the QuickChange site-directed mutagenesis kit according to the manufacturer’s instruction (Agilent Technologies). The list of primers used in this study was previously published.
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bioRxiv - Developmental Biology 2023Quote: ... RNA concentration and quality of the RNAseq libraries were assessed using a 2100 Bioanalyzer with the High Sensitivity DNA Kit (Agilent), and libraries were sequenced as single-end 50 bp reads on an Illumina HiSeq 2500.
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected with either EGFP-AKT2 or EGFP-AKT2+ HA-SIRT5 constructs or left untransfected and after 48 hours were subjected to the Mitostress assay kit (Cat# 103015-100) from Agilent, USA28.
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bioRxiv - Neuroscience 2023Quote: ... RNA quality of the tissue sections (RIN > 7) was confirmed using the Agilent RNA 6000 Nano Kit on the Bioanalyzer 2100 system (Agilent). Visium spatial gene expression slides were processed according to manufacturer instructions (10X Genomics ...
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bioRxiv - Cell Biology 2023Quote: ... vector containing the SARS-CoV-2 HA-ORF3a gene was used in site-directed mutagenesis using a QuikChange II site-directed mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA integrity was validated on a 2100 Bioanalyser with the Agilent RNA 6000 Nano kit (Agilent Technologies, Santa Clara, USA). Sequencing libraries were generated with the Illumina TruSeq RNA library Prep kit v2 (StarSEQ ...
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bioRxiv - Cell Biology 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). cDNA was synthesized using SMARTer Ultra Low RNA Kit for Illumina Sequencing (634936 ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis for Drp1 R456E and Y493A was completed to introduce each mutation into this construct individually using the QuikChange Lightning kit (Agilent) with primers from Integrated DNA Technologies (Coralville ...
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bioRxiv - Biophysics 2023Quote: ... derivative encoding two cysteine residues at positions 366 and 511 [constructed from plasmid pGEMD (-Cys)46 by use of site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit; Agilent) to replace codons 366 and 511 by a codon encoding cysteine residue] ...