Labshake search
Citations for Agilent :
6051 - 6100 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... RNA size and integrity were confirmed using RNA 6000 Pico kit for the Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) and Nanodrop™ ND-1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... vector encoding the pG gene as reported previously26 was site-specifically mutated by the insertion of an N-terminal serine using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent), according to the manufacturer’s instructions using following forward and reverse primers (ser codon underlined) ...
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bioRxiv - Genetics 2019Quote: ... Exons of DNA samples were captured using the in-solution enrichment methodology (Agilent SureSelect All Exon XT2 50 Mb Kit) and sequenced with an Illumina HiSeq 2500 instrument to generate 100 bp paired-end reads ...
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bioRxiv - Microbiology 2019Quote: ... Mutation of glycine at position 2 to alanine was accomplished using the Quick-Change Mutagenesis kit (Agilent, Santa Clara, CA) to generate ptubTgFBXO1HAG2A.
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bioRxiv - Genetics 2020Quote: ... WGS library-size selection was then verified using the 2100 Bioanalyzer DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA). The WGS library was then sequenced (2×150 bp ...
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bioRxiv - Genetics 2020Quote: ... in the nmt1-GFP-swi6+ expression construct (kindly gifted by Dr. Alison Pidoux) using the QuickChange site-directed mutagenesis kit (Stratagene), as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... plasmid using oligonucleotides containing the SHRF syndrome-linked G58V and G226D missense mutations and the QuikChange Site-Directed Mutagenesis Kit (Stratagene). The untagged RRP4/rrp4-G226D (pAC3656 ...
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bioRxiv - Genetics 2020Quote: The BRCA2 and PALB2 constructs were made siRNA resistant by introducing three silent mutations with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed mutagenesis of Aγ was performed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent, Santa Clara, CA). cRNAs were transcribed using mMessage mMachine Transcription Kits (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
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bioRxiv - Genomics 2021Quote: ... Concentration and quality of the libraries were measured using Qubit® 2.0 Fluorometer and Bioanalyzer High Sensitivity DNA Analysis kit (Agilent), respectively ...
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bioRxiv - Plant Biology 2021Quote: ... The total RNA quality was assayed using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies, St. Clara, USA). Labelling was done following the Low Input Quick Amp Labelling protocol of the Two-Color Microarray-Based Gene Expression Analysis system (Agilent Technologies ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: The first yeast library (called library A in this study) of FAST constructed by error-prone PCR using Genemorph II kit (Agilent) was previously described23 ...
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bioRxiv - Neuroscience 2021Quote: ... Both Southern blot probes were generated by standard PCR and subjected to random labelling using a Prime-It II Random Primer Labelling Kit (#300385, Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA integrity was determined by microfluidic capillary electrophoresis using the Agilent RNA 6000 Nano Kit and the 2100 Bioanalyzer Instrument (Agilent).
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bioRxiv - Molecular Biology 2020Quote: ... Mutation of the near-cognate codon AAG to CAG was performed using a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All generated constructs were verified by Sanger sequencing.
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bioRxiv - Microbiology 2021Quote: ... and RNA integrity was assessed using the RNA Nano 6000 Assay Kit for the Agilent Bioanalyzer 2100 system (Agilent Technologies). Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted RNA using the Qiagen RNeasy Blood and Tissue Kit and checked RNA integrity with a Bioanalyzer 2000 (Agilent) or TapeStation 2200 (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed using Illumina Nexterna XT kit and were analyzed for concentration by Qubit and for size distribution by Agilent Bioanalyzer ...
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bioRxiv - Neuroscience 2019Quote: ... RNA integrity was measured using Agilent 2100 Bioanalyzer System and Agilent RNA 6000 Pico Kit (Cat. No. 5067-1513, Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
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bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
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bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... The D238A catalytically deficient fbl mutant was generated by site-directed mutagenesis with the help of the QuikChange II site-directed mutagenesis kit (Stratagene) and primers LD3881 and LD3882 ...
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bioRxiv - Developmental Biology 2021Quote: ... or the Illumina TruSeq Stranded Total RNA Library Prep kit (medaka hybrid and mCherry or KDM4E-injected zebrafish samples) and checked using a Fragment Analyzer System (Agilent) before multiplexing ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: The pGEX6P1-Hecw C1394W construct was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit protocol (Agilent) using the following primers ...
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bioRxiv - Biochemistry 2021Quote: ... 67)) plasmid as PCR template Bri2 BRICHOS D148N was obtained with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, US), and the DNA sequence was confirmed (GATC Bioteq ...
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bioRxiv - Cancer Biology 2021Quote: ... The amount and average fragment length of amplified RNA was determined using the RNA 6000 Pico Kit (Agilent, 5067-1513) with a 2100 Bioanalyzer according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2021Quote: Mutations were introduced to LRH-1 in the pMSC7 and pCI vectors using the Quikchange Lightning Site-Directed Mutagenesis Kit as directed by the manufacturer (Agilent). Constructs were sequenced prior to use.
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bioRxiv - Biochemistry 2021Quote: ... Parkin mutant plasmids were cloned beginning with a Homo sapiens wild type eGFP-parkin vector [36] and performing PCR point mutagenesis (Quikchange Mutagenesis kit: Stratagene) utilizing primers ordered from Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... MYC-binding sites (E-boxes with the sequence CACGTG at positions -1149 bp and -1353 bp upstream of the transcription start site of the PTEN gene) were mutated to CAAGAA using Quikchange Lightening kit (Agilent). The TATA synthetic promoter sequence was derived from pGL firefly reporter with a minimal promoter.
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bioRxiv - Biochemistry 2020Quote: ... This construct was expressed in the HEK cell line and regenerated with 11-cis-retinal.22 MRh was prepared similarly.22 Site-directed mutagenesis was performed using the QuikChange Multisite-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... pCMVHA hEZH2 and V5-EZH2 vector was used to generate EZH2-H689A mutant vector using the mutagenesis primers listed in Table S1 with QuikChange II site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... DNA quality of the samples was assessed with the Fragment AnalyzerTM and the DNF-488 High Sensitivity genomic DNA Analysis Kit (Agilent). DNA was slightly more fragmented than defined by the quality control standards but this fragmentation was minor ...
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bioRxiv - Biophysics 2020Quote: A Pol I expression vector was generated from the plasmid pXS67 (Yale Coli Genetic Stock Center, Strain CJ803) by site-directed mutagenesis using a QuikChange kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... psub-201 [61] containing the full-length AAV2 genome as template and the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Biochemistry 2021Quote: ... AccuScript high-fidelity first-strand cDNA synthesis kit for reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was procured from Agilent.
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bioRxiv - Biochemistry 2021Quote: About 2 µg of obtained total RNA was immediately used for cDNA formation using AccuScript High Fidelity cDNA Synthesis Kit (Agilent). RT-qPCR was performed in a 96-well plate on a CFX96 qPCR system (BioRad) ...
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bioRxiv - Developmental Biology 2021Quote: ... pCS2+-5’HA-sobp and pCS2+-sobp-3’HA were generated using the QuikChange lightning Site-directed mutagenesis kit (Agilent). The same kit was used to sequentially remove three nucleotides located at the 5’ end of the pCS2+-3’HA-sobp ORF to generate a construct whose transcribed mRNA does not bind to the designed translation-blocking antisense morpholino oligonucleotide (pCS2+-sobpMOI-3’HA ...
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bioRxiv - Developmental Biology 2021Quote: ... the PCR products from the genotyping of the first outcross (generation F1) were cloned into the cloning vectors provided by the StrataClone PCR cloning kit (Stratagene). The cloning was performed according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: ... The ethanol was removed carefully and the resulting RNA extract was resuspended in 10 μL RNase-free water for immediate analysis (RNA 6000 Nano kit, Bioanalyzer instrument and the Bioanalyzer 2100 software, Agilent).
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bioRxiv - Evolutionary Biology 2021Quote: Cys to Ala substitution mutations were introduced at specific sites in the ectodomain of wild type toll by using QuickChange II XL site-directed mutagenesis kit (Stratagene) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Quality and quantity of fragmented DNA and the library for sequencing were analyzed by Bioanalyzer (Agilent High Sensitivity DNA kit). To identify variants from the whole genome sequencing ...
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bioRxiv - Microbiology 2021Quote: ... RNA integrity was assessed using the RNA Nano 6000 assay kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Libraries were prepared for sequencing by Cambridge Genomics Services using 3 μg of total RNA and an Ultra™ RNA library prep kit for Illumina (NEB ...
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bioRxiv - Genomics 2020Quote: ... The library preparations for the genome-wide data were performed according to the SureSelect XT Mouse Methyl-Seq Kit Enrichment System for Illumina Multiplexed Sequencing Library protocol (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... The indicated domain deletions and point mutations were generated by site-directed mutagenesis using the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent). For CD34+ HSPC cultures ...
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bioRxiv - Immunology 2020Quote: ... quantified by Nanodrop and the quality was assessed using an Agilent Bioanalyzer (Agilent, Cheshire, UK. The two-color low input Quick Amp Labelling kit (Agilent) was used to Cy3- or Cy5-fluorescently label cRNA samples ...
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bioRxiv - Microbiology 2021Quote: ... Insertion of the desired point mutations in S-RBD was done using QuickChange II site directed mutagenesis kit (Agilent Technologies). Primers for mutagenesis were designed using The QuickChange® Primer Design Program provided by manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... 6XHis tag was added to the N-terminus of Upf1 in pGEM-3Zf (+) using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) with oligonucleotides 5-N-His-UPF1 and 5-N-HIS-UPF1-r to yield pGEM3Zf(+)-6XHis-UPF1 ...