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Citations for Agilent :
6101 - 6150 of 7040 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Libraries were bisulfite converted with the EZ-96 DNA Methylation-Direct MagPrep kit and cleaned up using an automated liquid handling platform (Agilent Bravo). The bisulfite converted libraries were amplified for 12 cycles with KAPA HiFi HotStart Uracil+ ReadyMix (Roche) ...
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bioRxiv - Genomics 2022Quote: ... DNA integrity of the clinical samples was assessed with the Femto Pulse System using the Genomic DNA 165 kb Kit (Agilent Technologies) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Purified total RNA (100 ng) was labelled with Cyanine3 (Cy3) using a Low Input Quick Amp WT labelling kit (One-color; Agilent Technologies). The Cy3-labelled cRNA was checked for its amount (> 5 μg ...
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bioRxiv - Microbiology 2022Quote: ... RNA-Seq and ChIP-Seq libraries were quantified with the Qubit™ dsDNA HS Assay kit and their size distribution profiles recorded with the TapeStation 4200 System (Agilent) in combination with the D5000 DNA ScreenTape System (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... were prepared by double-stranded plasmid mutagenesis using the primer pairs listed in Table S1 and a Quikchange Site-Directed Mutagenesis Kit (Agilent Tech.). After verification by DNA sequencing ...
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PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaksbioRxiv - Molecular Biology 2019Quote: ... Three silent mutations (in capital, gctggaGatCAgGgagcaa) were introduced in the Flag-PHF2 plasmid using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies) to make it resistant to siRNA oligonucleotide PHF2#1 (Flag-PHF2*) ...
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bioRxiv - Molecular Biology 2019Quote: ... The quantity and quality of the pool of selected dsDNA oligos were determined using Qubit fluorometric assay and Agilent BioAnalyzer High-Sensitivity DNA kit (Agilent Technologies). Emulsion PCR and Ion Sphere Particles (ISP ...
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bioRxiv - Molecular Biology 2019Quote: ... The pGL3b-ChREBPβ-exon1b-luc reporter mutants Ebox-del (E-box deleted) and DR4-del (candidate DR4 response element deleted) were generated by using the QuikChange Site-directed Mutagenesis kit (Agilent Genomics) and the primers listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... two cysteine to alanine point mutations (C115A/C118A) in the DNA binding domain were introduced using the QuikChange Site-directed Mutagenesis kit (Agilent Genomics) and the primers listed in Supplementary Table 1 ...
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bioRxiv - Genomics 2019Quote: ... upon enzymatic fragmentation with SureSelect XT HS and XT Low Input Enzymatic Fragmentation Kit (G9702-90050, Revision A0, September 2018, Agilent Technologies), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... standard curve qPCR and an Agilent high sensitivity DNA kit with the Agilent 2200 Tapestation instrument (Agilent genomics, Santa Clara, US). Library preparation was carried out using dual-indexed forward and reverse primers ...
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bioRxiv - Genetics 2019Quote: ... Full-length cDNA was then synthesised with a mix of random and oligo (dT) using the AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Genomics) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2019Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Plant Biology 2019Quote: ... and the integrity was examined with the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was extracted from four samples using a Qiagen Gentra HMW kit resulting in molecules with a length of mainly > 48 kbp (TapeStation, Agilent Genomics). Further work was carried out by The University of Liverpool’s Centre for Genomic Research ...
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bioRxiv - Cancer Biology 2019Quote: ... Quality control and quantification of total RNA was carried out using the NanoDrop 1000 UV-Vis spectrophotometer (Kisker) as well as the Agilent 2100 Bioanalyzer with a RNA Nano 6000 microfluidics kit (Agilent Technologies).
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bioRxiv - Evolutionary Biology 2019Quote: ... The size distribution of the extracted gDNA was accessed using the genomic DNA kit on Agilent TapeStation 4200 (Agilent technologies, CA). A 10X Genomic library was constructed by using the Chromium Genome Reagent Kits v2 on 10X Chromium Controller (10X Genomics Inc. ...
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bioRxiv - Genetics 2019Quote: ... RNA quality was assessed on an Agilent 2100 Bioanalyzer utilizing the Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA) as well as a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... the point mutation K82R was introduced in the pFast-Bac HT-PLK1 vector using QuikChange XL site-directed mutagenesis kit (Agilent Technologies), see Table EV7 for primer sequences.
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bioRxiv - Cell Biology 2019Quote: ... and FACS-isolated eYFP positive cells (FACS ARIA IIu sorter, Becton Dickinson) were collected in Lysis Buffer (400753, Absolutely RNA Nanoprep kit, Agilent Technologies).
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bioRxiv - Developmental Biology 2019Quote: ... truncated and deletion versions of RAB13 3’UTR were generated by PCR using 0.3 μM sequence-specific primers (Extended Data Table 2) and Platinum Pfx DNA polymerase or using QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions and introduced into the pcDNA3-HBB-24XMS2SL-MCS using the NheI and XhoI sites.
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bioRxiv - Genomics 2019Quote: ... Each pool of RNA was individually labelled with Cyanine-3 and Cyanine-5 (Cy3 and Cy5) using the Low input Quick Amp Labelling Kit (Agilent Technologies) followed by purification through Qiagen RNeasy Columns (Qiagen ...
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bioRxiv - Genetics 2019Quote: ... The PAM sequences in the c(3)G gene were mutated using the Quik Change II XL Site-Directed Mutagenesis Kit (Agilent Technology). The bases changed are in bold above ...
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bioRxiv - Biochemistry 2021Quote: ... and PcrA V448C were generated by site-directed mutagenesis of the wild type construct using the Quikchange II XL kit (Agilent Technologies). In the last gel filtration chromatography of PcrA V448C ...
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bioRxiv - Genomics 2021Quote: ... Gene-specific probes were generated by random priming in the presence of ATP [α32P] using the Prime-It II Random Primer Labeling Kit (Agilent, 300385) using PCR generated DNA template produced from gDNA isolated from a wild type S.pombe strain (YP71 ...
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bioRxiv - Genomics 2021Quote: ... and library profile was assessed using a High Sensitivity DNA kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was sequenced on an Illumina NovaSeq instrument (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... The Drich and Reverse Drich CP mutants were made using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA). For the Drich mutant ...
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bioRxiv - Microbiology 2021Quote: Mutation of E2 residues 399YAL401 to AAA was achieved using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA). The following primers were used ...
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bioRxiv - Genomics 2021Quote: ... The libraries were quantified and qualified using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA analysis kit (Agilent, 5067-4626).
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bioRxiv - Cell Biology 2020Quote: ... residues at positions 29 and 30 and lysine residues at positions 33 and 34 to alanine (A) using the QuikChange Site-directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... To construct a plasmid encoding GAS6 tagged with Myc-His (pSecTag2-hGAS6-MycHis) the stop codon from pSecTag2-hGAS6 was removed using the QuickChange II XL Site-Directed Mutagenesis kit (Agilent Technologies). Next ...
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bioRxiv - Neuroscience 2020Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Genomics 2021Quote: ... Final libraries were evaluated using fluorescent-based assays including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100). Libraries were sequenced on an Illumina NovaSeq 6000 system using 2 x 150bp cycles.
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bioRxiv - Neuroscience 2020Quote: ... Primary myotubes underwent assessment of key parameters of glucose and fatty acid oxidation dependency and capacity using the Seahorse Mito Fuel Flex Test Kit (Agilent Technologies).
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bioRxiv - Neuroscience 2020Quote: The dependence and capacity of EDL fibers to use glucose and fatty acid as fuel substrates was determined using the Seahorse XF Mito Fuel Flex Test Kit (Agilent Technologies). Prior to the assay ...
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bioRxiv - Neuroscience 2020Quote: ... The integrity and concentration of the extracted RNA were assessed using an Agilent RNA 6000 Nano Kit on a 2100 Bioanalyzer (Agilent, USA). Based on RNA integrity number (RIN > 7.2 ...
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bioRxiv - Microbiology 2021Quote: Mutations in AdMarRars were introduced by site-directed mutagenesis using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The mutagenic oligonucleotides used for both strands and the respective changes introduced (underlined ...
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bioRxiv - Cancer Biology 2022Quote: ... An aliquot of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Agilent).
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bioRxiv - Cancer Biology 2022Quote: ... Finally their fragment size was analyzed by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Agilent). 2×150 bp paired-end reads were sequenced on an Illumina HiSeq 2500 with a target depth of 60 million reads per sample.
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bioRxiv - Biochemistry 2020Quote: ... The mutations to DrBphP (H533D, E536A) and for AgP1 (D529H) were introduced with QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies). For cloning the chimera construct ...
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bioRxiv - Bioengineering 2020Quote: ... respectively in ThuE were designed by site-directed mutations in optical sucrose sensors by using the QuickChange II Site-Directed Mutagenesis (SDM) Kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Biochemistry 2020Quote: ... Histone H3 bearing K36 substituted with cysteine (K36C) was generated by site-directed mutagenesis using an Agilent Quikchange XL Site-Directed Mutagenesis kit (Agilent #200517) with the canonical H3 gene in pET3 vector ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were prepared according to the instruction of the Agilent protein 80 kit and analyzed on a 2100 Bio-analyzer device (Agilent technologies). The ATPase activity of FSS-PscN was determined by measuring the release of total phosphate at 37°C for 30 minutes which was detected by the malachite green method (Casabona et al. ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA integrity was evaluated using the RNA Nano 6000 Assay Kit for a Bioanalyzer 2100 System (Agilent Technologies, CA, USA). The complete circRNA study involved the following steps ...
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bioRxiv - Molecular Biology 2020Quote: ... pET29b-PyoS8-ImS8 was used as a template for the site-directed mutagenesis (QuikChange XL site-directed mutagenesis kit; Agilent Technologies) to yield pET29b-PyoS8Glu736Ala-ImS8.
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bioRxiv - Cancer Biology 2021Quote: Comparative gene expression profiling of the prostate cancer cells starved for glutamine for 24 h or cultured with glutamine supplementation was performed using SurePrint G3 Human Gene Expression 8×60K v3 Microarray Kit (Design ID 039494, Agilent Technologies) according to manufacturer’s recommendations as described previously 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Compound incubated plates and basal plates were then washed with assay media (Seahorse XF DMEM assay media kit (Agilent; #103575-100), 10 mM glucose ...
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bioRxiv - Cell Biology 2021Quote: ... except that in this case the cDNA was synthesized from 1 µg (testis) or 13-20 ng (spermatozoa) of RNA using the AccuScript High-Fidelity 1st Strand cDNA Synthesis Kit (Agilent 200820) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...