Labshake search
Citations for Agilent :
5551 - 5600 of 5718 citations for Rat Cystatin S CST4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... early and late nascent strands were labeled with Cy3 and Cy5 ULS molecules using the Genomic DNA labeling Kit (Agilent, Santa Clara, CA, USA) following manufacturer's instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmid DNA mutations in the predicted orthosteric binding site of PAR4 were generated using the QuikChange XL Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Physiology 2019Quote: ... RNA quality and integrity were validated using a NanoDrop ND-1000 UV–vis Spectrophotometer (NanoDrop Technologies, Wilmington, USA) and Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, USA) respectively ...
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bioRxiv - Immunology 2019Quote: Various N-linked glycosylation site mutants of UL144 were generated by site-directed mutagenesis using Quick Change II site directed mutagenesis Kit (Stratagene, La Jolla, CA, USA). The Asn residues carrying the N-linked glycan were mutated individually or in combination at residues N61 ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatants were transferred to new tubes and 50 µl of each cleared lysate was mixed with 50 µl Lysis Buffer (0.7 µl β-mercaptoethanol/100 µl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at –80° for later preparation as input RNA ...
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bioRxiv - Genomics 2021Quote: ... Libraries from all sample sets were quantified on the 2100 Bioanalyzer instrument with the Agilent DNA 7500 Kit (both Agilent Technologies, Santa Clara, CA).
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bioRxiv - Bioengineering 2022Quote: ... Size pattern was assessed with Fragment Analyzer-96 with the DNF-474-0500 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies, DNF-474-0500). Libraries (Average size ...
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bioRxiv - Biochemistry 2021Quote: Mutated forms of rat and human BDH2 enzymes (Y157F and Y147F, respectively) were generated by site-directed mutagenesis using a QuikChange II XL kit (Stratagene, La Jolla, CA, USA), with pCOLD I/rBDH2 and pCOLD I/hBDH2 plasmids as templates and mutagenic primers Y157F-rBDH2-S and Y157F-rBDH2-AS as well as Y147F-hBDH2-S and Y147F-hBDH2-AS ...
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bioRxiv - Neuroscience 2020Quote: ... the purity and concentration of RNA samples were determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and Ile-residues of the HXXXDX motif of ZmGL2 and ZmGL2-LIKE were generated using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The ZmGL2 protein was also expressed in E ...
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bioRxiv - Systems Biology 2020Quote: ... The sub-libraries were each resuspended to a final volume of 20 μL in nuclease-free water and fragment distribution was checked running a bioanalyzer (Agilent High Sensitivity DNA Kit) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... The fragment sizes and concentrations of the pooled samples were determined on a Fragment analyzer 5200 using the DNF-473-Standard Sensitivity NGS Fragment Analysis Kit (Agilent, Santa Clara, CA, USA). The indexing PCR was performed under the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equal quantity and combined to multiplex to make a final library and QC was done again using Qubit quantification kit and Agilent bioanalyzer using high sensitivity DNA chip (Agilent Biotechnology, catalog # 5067-4626). The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using High output 300-bp Single end Read sequencing kit.
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bioRxiv - Plant Biology 2021Quote: ... Other related alleles required for transient expression or infection analyses were either created using the QuickChange Site-directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) or synthesised by Twist Bioscience (South San Francisco ...
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bioRxiv - Neuroscience 2022Quote: ... with the standard-sensitivity RNA chip or an Agilent 2100 Bioanalyzer system with the RNA 6000 Nano Labchip Kit (Agilent Biotechnologies, Palo Alto, USA) was used to analyze the extracted RNA samples ...
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bioRxiv - Genomics 2022Quote: ... The obtained library quality and concentration was assessed by monitoring the distribution of the fragment sizes with a Bioanalyzer 2100 instrument using RNAnano reagent kit (Agilent Technologies, Santa Clara, CA). This size and quantity information was used for pooling the libraries in equimolar concentrations to normalize each library ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA quality was assessed by determining RNA integrity numbers (RINs) using the Agilent 2100 Bioanalyzer system with the Agilent RNA 6000 Nano Chip Kit (Agilent Technologies, Santa Clara, California).
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bioRxiv - Immunology 2022Quote: ... for fragment size using the High Sensitivity NGS Fragment Analysis kit on a Fragment Analyzer capillary electrophoresis system (catalog # DNF-474-0500; Agilent, Santa Clara, CA, USA), and functional validation using the Universal Library Quantification kit (catalog # 07960140001 ...
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bioRxiv - Immunology 2022Quote: ... and for integrity using the Standard Sensitivity RNA Analysis kit on a Fragment Analyzer capillary electrophoresis system (catalog # DNF-471-0500; Agilent, Santa Clara, CA, USA). A total of 250ng total RNA was used for each sample as input to the TruSeq Stranded Total RNA library prep kit with unique-dual indexing (catalog #s 20020598 & 2002371 ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 0.3 μg of total RNA using the Affinityscript qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, US). qPCR was performed using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA extraction was performed using a FinePure Plant RNA Kit (Genfine Biotech Co. Ltd., Beijing, China) and the qualities were assessed using a Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA). A cDNA library was constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... RNA integrity (RIN) was assessed using a Bioanalyzer 2100 instrument in conjunction with the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, California).
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bioRxiv - Plant Biology 2022Quote: ... The RNA quality was assessed using both the NanoPhotometer ® spectrophotometer (IMPLEN, CA, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were constructed using NEB Next® UltraTM RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2022Quote: ... RNA samples were then quantified and checked for RNA integrity using an Agilent BioAnalyzer 2100 with RNA Nano 6000 kit (Agilent Technologies, Santa Clara, USA), and only samples with an RNA integrity number ≥8 were sent for RNA-Seq ...
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bioRxiv - Cell Biology 2022Quote: ... I15K/R54P) were generated by mutagenesis of pcDNA-3XFLAG-TRIM25 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA), while the TRIM25-PTAA mutant was generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values were > 7 for all samples ...
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bioRxiv - Neuroscience 2021Quote: ... The quality and quantity of the extracted RNA samples were analyzed with a 2100 Bioanalyzer using an RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA). Paired-end cDNA libraries were prepared from 0,2 ng of extracted RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed as previously described (Sodi et al., 2015) using Brilliant II qRT-PCR Master Mix 2 Kit (Stratagene, San Diego, CA, USA) using Applied Biosystems 7500 machine. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples from Tenebrio molitor and Calliphora vicina were qualified with the High Sensitivity RNA ScreenTape Assay Kit and the Agilent 4200 TapeStation system (Agilent, Santa Clara, United States) and Pyrrhocoris apterus samples with the Agilent RNA 6000 Pico Kit on a BioAnalyzer 2100 (Agilent ...
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bioRxiv - Immunology 2022Quote: ... Individual indexed IGK and IGL AIRR-seq libraries were assessed using the Agilent 2100 Bioanalyzer High Sensitivity DNA Assay Kit (Agilent, Cat. No. 5067-4626) and the Thermofisher Qubit 3.0 Fluorometer dsDNA High Sensitivity Assay Kit (Thermofisher ...
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bioRxiv - Immunology 2022Quote: ... Phe322 was substituted for the Tyr322 residue of WT and G307S DNAM-1 by using QuikChange II Site-Directed Mutagenesis Kits (Agilent technologies, Palo Alto, CA) with primers (5′-GAGAGGATATTTTTGTCAACTATCCAACC-3′ and 5′-GGTTGGATAGTTGACAAAAATATCCTCTC-3′) ...
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bioRxiv - Neuroscience 2022Quote: ... cDNAs were synthesized through reverse transcription from the total RNA according to the protocol of Affinity Script Multiple Temperature cDNA synthesis kit (Agilent Technologies, Santa Clara, CA). Expression levels of genes encoding plp (forward primer ...
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bioRxiv - Plant Biology 2019Quote: ... Additional synthetic SlMai1 variants were generated by PCR amplification using oligonucleotides encoding the amino acid changes followed either by BP Clonase recombination (for G2A and C3/4S mutants) or using the QuickChange Site-Directed Mutagenesis kit (for K91M; Agilent, Santa Clara, CA, USA).
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bioRxiv - Cancer Biology 2019Quote: ... we modified the PTK-ATF4-Luc plasmid from (Dey et al., 2010)39 using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent; Santa Clara, California, USA) to introduce two BsmBI restriction sites flanking the ATF4 5’ UTR ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasmid DNA mutations in the C-terminus of PAR2 were created using QuikChange XL Multi Site-Directed Mutagenesis kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Cancer Biology 2020Quote: ... Target capture were performed on pools of 96 libraries using a Sure Select® user-defined probe kit (Agilent Technologies, Palo Alto, CA, USA). Massively parallel sequencing was carried out in a High-Seq® machine (Illumina ...
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bioRxiv - Cancer Biology 2019Quote: The pEGFP-N1-CELF1 putative eIF4E binding mutants were generated via site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, La Jolla, CA) and primers listed in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2019Quote: We used the manufacturer protocol for the Agilent SureSelect Capture kit for non-human exomes with 200 ng gDNA per individual (75 Mb capture designed on the zv9 version of the zebrafish genome; Agilent SSXT Zebrafish All Exon kit; Agilent Technologies, Santa Clara, CA). Samples were multiplexed and run across two lanes of the Illumina HiSeq 4000 as paired-end 150 bp reads ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Neuroscience 2019Quote: ... and SNAREQ (SNAP25 K40A/D51A/E52A/E55A/D166A) were generated in the above described Syt1 and t-SNARE background respectively using the QuickChange mutagenesis kit (Agilent Technologies, Santa Clara, CA). All proteins were expressed and purified as described elsewhere (Mahal et al ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutants in the VPg cistron were synthesized by long inverse site-directed PCR mutagenesis using the QuickChange II XL Kit (Stratagene, San Diego CA, USA) following the manufacturer’s instructions and using p35STunos as template ...
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bioRxiv - Microbiology 2021Quote: ... The purity and integrity of the RNA pools were checked by the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and the RNA Nano 6000 Assay Kit and Bioanalyzer2100 system (Agilent Technologies, CA, USA). One microgram of RNA from each pool was used for library preparation with the NEBNext® UltraTMRNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid phapXVENUS-hph (Gsaller et al., 2014) was used as template and modified using QuikChange II site-directed mutagenesis kit (Agilent for K38E and R50A) or Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... The fragment distribution of each library was assessed the using the High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Libraries were quantified prior to sequencing using the Qubit DNA High Sensitivity kit (ThermoScientific ...
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bioRxiv - Genomics 2021Quote: ... exome enrichment was applied to the cDNA purified from the previous step to enrich spliced cDNA by using probe kit SSELXT Human All Exon V8 (Agilent, catalog no. 5191-6879) and the compatible reagent kit SureSelectXT HSQ (Agilent ...
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bioRxiv - Immunology 2021Quote: cDNA was synthesized from the previously extracted RNA of mice colon tissue by using Affinity Script One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA). RNA was mixed with random 9mer primers ...
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bioRxiv - Molecular Biology 2021Quote: ... after the molecular cloning the wt sequence was restored by mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, CA), the pETM11-CspA plasmid as DNA template and the mutagenic primers G670 5′-TTTCAGGGCGCCATGTCCGGTAAAATGACTG-3′ and G671 5′-CAGTCATTTTACCGGACATGGCGCCCTGAAA-3′.
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bioRxiv - Cell Biology 2021Quote: ... pCMV6-XL5-WT-SorLA759 or pCMV6-XL5-WT-SorLA2131 plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent (Santa Clara, CA, USA) according to manufactory’s instructions ...