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Citations for Agilent :
5501 - 5550 of 5718 citations for Rat Cystatin S CST4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... RNA concentration and integrity were measured using the RNA Pico 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA), and only samples with RIN > 8 were included in RNA-seq ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality of the RNA samples (RIN score >7) was verified on the Agilent 2100 Bioanalyzer using the RNA 6000 kit (Agilent, cat# 5067-1513). 1 µg of each RNA sample was then used for poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA was used as input material for RNA sample preparations ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Physiology 2023Quote: ... Point mutations were generated using the QuikChange II XL Site-Directed Mutagenesis or Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The integrity of the RNA samples was confirmed using the Agilent Bioanalyzer 2100 with an RNA 6000 Pico Kit (Agilent, Santa Clara, CA). All samples had an RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2022Quote: ... followed by quality check (QC) and fragment size distribution using a High Sensitivity Tape station Kit (Agilent 2200, #5067-5584 and #5067-5585). The library was sequenced using the HiSeq 4000 Illumina platform.
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bioRxiv - Immunology 2022Quote: ... reverse transcription was carried out with 500ng of RNA using an Affinity Script qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the libraries were checked for quality and size distribution using an Agilent 2100 Bioanalyzer and the High sensitivity DNA kit (Agilent, San Diego, USA). Quantitation of the library was performed by Qubit using the Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified DNA library was analyzed using an Agilent 2100 bioanalyzer with an Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA) and a Qubit 2.0 with Qubit 1X dsDNA HS Assay Kits (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and quality was measured using the RNA 6000 Pico kit on the Bioanalyzer 2100 capillary electrophoresis system (Agilent, Santa Clara, CA) and stored at −80°C ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutations were introduced into ATP8B2 and ATP8B4 using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) and/or the SLiCE cloning method (Zhang et al ...
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bioRxiv - Bioengineering 2023Quote: ... The RNA quality was evaluated using a 5200 Fragment Analyzer System and an Agilent HS RNA Kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were constructed Using MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... The total amount and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... The total RNA quantity and purity were analyzed by using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511). RNA-seq libraries were constructed and then sequenced using an Illumina Novaseq™ 6000 (LC-Bio Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... two successive rounds of cysteine to serine mutagenesis were performed using the QuikChange Lightning Multi Site-directed Mutagenesis kit (Agilent Technologies #210513/#210515) according to manufacturer’s instructions at 1/4th scale.
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bioRxiv - Systems Biology 2023Quote: ... quality check and quantification of total RNA was done using the Agilent Bioanalyzer 2100 in combination with the RNA 6000 pico kit (Agilent Technologies, 5067-1513). Total RNA library preparation was done by introducing 500 ng total RNA into Illumina’s NEBNext Ultra II directional mRNA (UMI ...
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bioRxiv - Physiology 2023Quote: ... Quality control of the total RNA submissions was completed using the Agilent TapeStation 4200 using the RNA ScreenTape Assay kit (Agilent Technologies 5067-5576). Total RNA (500ng ...
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bioRxiv - Microbiology 2023Quote: ... tissues were incubated with proteinase K diluted 1:10 in 1X TBS (Fisher Scientific, Cat. #BP24711; and PNA ISH kit, Agilent Dako, Cat. #K5201) for 10 min at RT in a humidity chamber ...
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bioRxiv - Genetics 2023Quote: ... Double-stranded cDNA was synthesised from 2 μg of total RNA using AccuScript High Fidelity 1st Strand cDNA Synthesis Kit (Stratagene, La Jolla, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies, Santa Clara, CA, USA) to determine the length distribution of the fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... The exome region of the whole genome libraries was enriched with the Agilent SureSelectXT Human All Exon Kit V6 (Agilent; Santa Clara, CA) and sequenced on the NovaSeq 6000 Sequencing System (Illumina ...
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bioRxiv - Genomics 2022Quote: ... we assessed the quality of the RNA by quantifying the RNA integrity number (RIN) for each sample using the Agilent RNA 6000 Pico kit (Agilent, Santa Clara, CA) on the Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... The human GABAA receptor α1 subunit missense mutations (S76R, R214C, D219N, G251D, P260L, M263T, T289P, and A322D) were constructed using QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). Enhanced cyan fluorescent protein (CFP ...
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bioRxiv - Physiology 2023Quote: ... and the quality (e.g. fragment length) was assessed for a subset using a Bioanalyzer High Sensitivity DNA chip kit (Agilent Technologies, Santa Clara, CA). Single-end sequencing with 100-bp read length was conducted on a NovaSeq platform (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... 10ug of total synaptosomal protein was plated in each well of a polyethyleneimine coated 96 well plate (n=5-6/animal) and analyzed using the MitoStress kit from Agilent (Santa Clara, CA). The MitoStress kit measures oxygen consumption rate (OCR ...
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bioRxiv - Molecular Biology 2023Quote: ... quantitation and sample purity were assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Beads-based double size selection was performed to ensure the fragment size of each sample was between 300-600 bp as measured by an Agilent DNA High Sensitivity NGS Fragment Analysis Kit (Agilent, DNF-474-0500). The concentration of ligated fragments in each library was quantified with the KAPA Library Quantification Kits for Illumina platforms (Roche/KAPA Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... and leucine (Leu41) within the conserved destruction box were mutagenized to alanine (RxxL-> AxxA) using the Quickchange mutagenesis kit (Stratagene, La Jolla, CA). Worms were transformed with the pJK3 plasmid carrying gfp::ify-1dm using microparticle bombardment as previously described (Praitis et al. ...
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bioRxiv - Plant Biology 2023Quote: ... The integrity and concentration of the RNA were accurately measured using the Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). The detection indicators included RIN value ...
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bioRxiv - Genomics 2023Quote: ... Libraries were eluted in 15µL H20 and concentrations measured with an Agilent 2100 Bioanalyzer using an Agilent HS DNA kit (Agilent Technologies, Cat# 5067-4627). Barcoded libraries of PV+ ...
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bioRxiv - Plant Biology 2024Quote: ... and RNA integrity and concentration were measured using the RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 System (Agilent Technologies, CA, USA). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... To determine the RNA integrity number the isolated RNA was run on an Agilent Bioanalyzer 2100 using an Agilent RNA 6000 Nano Kit as recommended by the manufacturer (Agilent Technologies, Waldbronn, Germany). Remaining genomic DNA was removed by digesting with TURBO DNase (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... The quality of the barcoded cDNA libraries was assessed with the High Sensitivity DNA Analysis Kit in the Agilent 2100 Bioanalyzer (Agilent Technologies, United States) and then sequenced (150 bp paired-end reads ...
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bioRxiv - Cell Biology 2024Quote: ... a DeNovix DS-11 FX spectrophotometer and an Agilent FEMTO Pulse System with a Genomic DNA 165 kb Kit (Agilent, FP-1002-0275), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA integrity was assessed using the Agilent TapeStation 4200 and the Agilent RNA ScreenTape Assay Kit (Agilent; 5067-5576 and 5067-5577).
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bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: The oxygen consumption rate (OCR) of cells was measured using the Seahorse Cell Mito Stress Test kit following the user guide (Agilent, cat. 103010-100). Briefly ...
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bioRxiv - Genetics 2024Quote: ... poly(A)+ RNA was separated and purified from total RNA using an oligo(dT) cellulose column Poly(A) Quick mRNA Isolation Kit (Stratagene, La Jolla, CA). Single-stranded cDNAs were synthesized by reverse transcription reaction using 0.25 U AMV reverse transcriptase (Life Sciences Advanced Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... while the concentration and integrity of RNA samples were detected by using Agilent 2100 RNA nano 6000 assay kit (Agilent Technologies, CA, USA). Subsequently ...
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bioRxiv - Biophysics 2024Quote: Introduction of the 118C mutation into the T4L gene (pET11a vector) was accomplished using the QuikChange site-directed mutagenesis kit (Agilent; Santa Clara, CA) and confirmed by DNA sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... Average fragment lengths of 40-50 kbp were confirmed using a Fragment Analyzer (Advanced Analytical) with an HS Large Fragment 50 Kb analysis kit (Agilent, DNF-464-33). Samples were prepared for Oxford Nanopore Technologies (ONT ...
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bioRxiv - Genetics 2024Quote: ... RNA concentration and quality was assessed using an Agilent 2100 Bioanalyzer with the RNA Nano 6000 kit (Agilent Technologies, Santa Clara, CA, USA), and only RNA samples with RIN >7 were processed for subsequent cDNA library construction and sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... Library qualities were assessed using the Agilent High Sensitive DNA Kit (#5067-4626) on an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). Qualified libraries underwent paired-end sequencing on an Illumina NovaSeq System (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 8.2-10 for all samples ...
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bioRxiv - Genetics 2021Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 7.3–10 for all samples ...
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bioRxiv - Developmental Biology 2020Quote: ... analysis was performed using TransStart Tip Green qPCR SuperMix kit (TransGen Co.) on a Stratagene Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) under the conditions of 94 °C for 30 s ...
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bioRxiv - Genomics 2019Quote: ... and successful size-selection verified by electrophoresis on an Agilent TapeStation using the Genomic DNA High ScreenTape kit (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Standard quality control steps were included to determine total RNA quantity and quality using Agilent 2100 Bioanalyzer with Eukaryote Total RNA Pico Kit and High Sensitivity DNA Assay (Agilent Technologies, Santa Clara, CA). Libraries were prepared using the TruSeq Stranded mRNA Library preparation Kit (Illumina Inc. ...