Labshake search
Citations for Agilent :
5201 - 5250 of 7040 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... The sections were incubated in the kit polymer-HRP anti-mouse (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark), as secondary antibody ...
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bioRxiv - Plant Biology 2019Quote: ... The phosphomimetic substitutions were introduced using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA). Resulting entry vectors were transferred into pGWB550(4 ...
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bioRxiv - Genomics 2019Quote: ... and RNA quality was verified using RNA 6000 Nano kits on an Agilent 2100 Bioanalyzer (Agilent Technologies, Mississauga, ON). RNA abundance levels were assayed using Affymetrix Mouse Gene 1.1 ST arrays (Affymetrix Mouse Gene 2.0 ST arrays were used for the C57BL/6 mice for EXP1 ...
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bioRxiv - Epidemiology 2019Quote: ... The average size of the library was determined by the Agilent High Sensitivity DNA Kit (Agilent, Santa Clara, CA). An accurate library quantification was determined using the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems ...
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bioRxiv - Systems Biology 2019Quote: ... Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina ...
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bioRxiv - Plant Biology 2019Quote: ... The quality and quantity of each library were determined using a Bioanalyzer with High Sensitivity DNA kit (Agilent Technologies), and KAPA Library Quantification Kit for Illumina (Illumina) ...
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bioRxiv - Developmental Biology 2019Quote: The exonic sequences were captured with the Agilent Sure Select All Exon v4 kit (Agilent, Santa Clara, CA, USA) and sequencing was performed on an Illumina HiSeq2000 sequencing apparatus (Illumina ...
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bioRxiv - Genetics 2019Quote: ... Serine-to-alanine mutation was generated using QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, CA, USA 200522) following manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... and AFF4 NM_014423.4:c.772C>T mutations were engineered using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... coli were generated by introducing the corresponding mutations in plasmid pDL893 using the QuikChange site-directed mutagenesis kit (Stratagene).
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bioRxiv - Genetics 2020Quote: The verification of average size of the DNA fragments using the Agilent DNA 12,000 kit and the 2100 Bioanalyzer System (Agilent) equipment ...
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bioRxiv - Genetics 2020Quote: ... RNA quality and quantity were respectively assessed using Agilent RNA 6000 Pico kit on Agilent 2100 Bioanalyzer (Agilent Technologies) and Qubit RNA HS assay kit on Qubit 3.0 Fluorometer (Thermo Fischer Scientific) ...
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bioRxiv - Genetics 2021Quote: ... RNA sample labeling for the Agilent array was done with the Agilent One-Color Microarray-Based Exon Analysis Low Input Quick Amp WT Labeling kit and the arrays were processed on the Montreal heart institute (MHI) Integrative Biology Platform’s Agilent system (Agilent Microarray Hybridization oven and SureScan Microarray scanner ...
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bioRxiv - Cell Biology 2020Quote: ... Visualization was completed using the DAB substrate reagent from above mentioned Kit (Agilent/Dako, Cat. no. GV800+GV809/821). Information about the immunohistochemical stainings obtained with courtesy from the Human Protein Atlas61.
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bioRxiv - Cell Biology 2020Quote: ... Visualization was completed using the DAB substrate reagent from above mentioned Kit (Agilent/Dako, Cat. no. GV800+GV809/821). Information about the immunohistochemical stainings obtained with courtesy from the Human Protein Atlas61.
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Total RNA integrity was determined using Agilent bioanalyzer (2100 Bioanalyzer system) using RNA 6000 Nano Kit (Agilent, 5067-1511). Library preparation was performed using 500 ng of total RNA ...
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bioRxiv - Microbiology 2021Quote: ... the quality of RNA (RNA integrity number from 7.5 to 9.4) was examined using the BioAnalyzer 2100 system with an RNA 6000 Nano Kit (Agilent, USA) before using the Ribo-Zero rRNA removal kit (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... while libraries with mutations at multiple locations were generated by QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Physiology 2021Quote: ... The length from RNA fragments was evaluated using the Agilent Pico Kit and the Agilent 2100 BioAnalyzer (Agilent Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... the quality of the library was tested by the Agilent Bioanalyzer 2100 kit (Agilent Technology, Inc., Santa Clara, CA). Sequencing was performed on the PacBio RS II instrument as per the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2020Quote: ... following manufacturer’s instructions and the quality or fragment size was assessed using the Bioanalyser High Sensitivity DNA kit (Agilent). 2nM of 400-500bp DNA library was sequenced using Illumina NextSeq500 platform to obtain paired-end 150bp reads.
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bioRxiv - Genetics 2021Quote: ... Target sequencing was performed for all the exons of 550 cancer-related genes (Agilent SureSelect XT Custom kit; Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... pCDNA3/Flag-HA-AKT1 15G/K mutant was generated by site mutagenesis using QuickChange Lightning Site-Directed Mutagenesis Kit following the manufacturer’s protocol (Agilent). Primers introducing mutation sites were designed by Agilent PrimerDesign Program on Agilent website listed in Table 3 ...
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bioRxiv - Cancer Biology 2020Quote: ... and S311A mutations were introduced by site directed mutagenesis using the QuikChange II Site-directed mutagenesis kit (Agilent #200521) and the following primers:
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bioRxiv - Developmental Biology 2020Quote: Total RNA obtained from FACS-sorted E7.5 mouse embryonic populations was purified with the Absolutely RNA nanoprep kit (Agilent) and quantified as above ...
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bioRxiv - Biophysics 2020Quote: ... Mutagenesis of of tubbyCT was done using QuikChange II XL Site-Directed mutagenesis kit (Stratagene, Agilent Technologies, Waldbronn, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Mutations in the AAD of TopBP1 were generated using the “QuickChange Multi Site-directed mutagenesis kit” (Agilent technologies, C200515): W1145R with primer 3 ...
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bioRxiv - Cell Biology 2020Quote: ... RNA isolate quality was assessed with an Agilent Bioanalyzer 2100 on using the RNA Pico Kit (Agilent, 5067-1513). 10ng of each RNA isolate was then prepared using the Takara SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara ...
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bioRxiv - Genetics 2021Quote: ... pellets were harvested by centrifugation and resuspended in 0.25 mL of TRIzol Reagent or 0.1 mL of Absolutely RNA Nanoprep Kit (Agilent #400753) lysis buffer ...
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bioRxiv - Genetics 2020Quote: ... Synthesis of cDNA was carried out with the AccuScript Hi-Fi cDNA Synthesis Kit (Agilent Technologies, Santa Clara, USA) using Oligo(dT ...
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bioRxiv - Genomics 2020Quote: ... The quality of the extracted RNA samples was assessed with an Agilent RNA 6000 Nano Kit (Agilent Technologies, USA) using a 2100 Bioanalyzer.
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bioRxiv - Immunology 2020Quote: ... total RNA was analyzed with the RNA Nano 6000 Kit on a Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Transcript levels were determined via qPCR using the THUNDERBIRD SYBR qPCR Mix kit (Toyobo) and Mx399P QPCR system (Agilent). The expression of either the UBQ10 or HEL gene was used as an internal control ...
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bioRxiv - Microbiology 2020Quote: ... and quality checked using a High Sensitivity DNA kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Sequencing was performed on the Illumina MiSeq platform using the MiSeq Reagent Kit v3 (600 cycles ...
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bioRxiv - Microbiology 2020Quote: The plasmid HIV-1ΔEnv INHA (D116A)ΔNef was obtained by insertional mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent). HIV-1 viruses were produced by cotransfection with calcium phosphate with 10 μg HIV-1 LAI (BRU ...
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bioRxiv - Immunology 2021Quote: ... total RNA from PBMC’s from 2 ml of blood was extracted using AllPrep DNA/RNA Micro Kit from Agilent Technologies (Cat ...
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bioRxiv - Immunology 2021Quote: ... The site directed mutagenesis was conducted with QuikChange II Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA, United States) in accordance with the manufactur’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Mutations were introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518 ...
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bioRxiv - Microbiology 2020Quote: ... ZAP mutants (W611A, Q668A, and Q668R) were generated by using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies). E ...
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bioRxiv - Immunology 2020Quote: Point mutations by site-directed mutagenesis were introduced in env constructs using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... the following mutations were introduced into the BG505 SOSIPv5.2 construct(17) using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent): P240T ...
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bioRxiv - Cell Biology 2020Quote: Glycolysis stress test: Agilent Seahorse XF Glycolysis Stress Test Kit was used as per the standard protocol by Agilent Seahorse (https://www.agilent.com/en-us/agilent404?s=www.agilent.com/cs/library/usermanuals/public/XF_Glycolysis_Stress_Test_Kit_User_Guide.pdf) ...
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bioRxiv - Cell Biology 2021Quote: ... The catalytically inactive ATGL(S47A) mutant was was made by site directed mutagenesis using the QuickChange II kit (Stratagene). PLIN2-GFP was a generous gift from Carole Sztalryd (University of Maryland) ...
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bioRxiv - Immunology 2021Quote: ... After 2h of AIM2 activation cells were processed per manufacturer’s directions and analyzed using the Agilent Seahorse Mito Stress Test kit (Agilent). For over-night stimulation with LPS cells were treated 2h after plating with 10 ng/mL LPS and incubated overnight at 37°C.
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bioRxiv - Physiology 2020Quote: Hippocampal total RNA was isolated using an Agilent total RNA isolation mini kit following the manufacturers’ instructions (Agilent, USA). The amounts of RNA were quantified by a nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Single base-pair SMD-CRD missense variants were then introduced using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, Cat # 200521 ...
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bioRxiv - Genetics 2019Quote: ... RNA integrity and concentration were assessed using the Bioanalyzer 2100 with the RNA 6000 Nano Lab Chip kit (Agilent) and the ND-1000 UV-visible light spectrophotometer (NanoDrop Technologies).
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bioRxiv - Genetics 2020Quote: ... enrichment and elution were performed according to manufacturer’s instructions and protocols (SureSelect Human All Exon Kits Version CRE, Agilent). The library was sequenced on an Illumina HiSEQ 2500 as paired-end 75bp reads ...
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bioRxiv - Molecular Biology 2020Quote: ... and the anticodon stem deletion in the tDNA genes was made by QuickChange lightning site-directed mutagenesis kit (Agilent) using the following primers:
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bioRxiv - Microbiology 2022Quote: ... the vector pEC1_ptpA previously constructed [22] was used as template to generate the suicide plasmid pEC1_ptpA_D120A derivative by using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies) with the primer #1559 (5’-GGAAGAGAGTGATGTACCAGCTCCATACTACACGAATAATT-3’) ...