Labshake search
Citations for Agilent :
5101 - 5150 of 7040 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The LaGH13_31B Y295A variant was generated using primers in Table S3 and the QuickChange II Site-Directed Mutagenesis kit (Agilent) with pET-28a(+)-LaGH13_31B as template ...
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bioRxiv - Biochemistry 2020Quote: MRG15 W53A was generated by site-directed mutagenesis using an Agilent Quikchange XL Site-Directed Mutagenesis kit (Agilent #200517) with the MRG15 WT in pGEX-6P-3 vector ...
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bioRxiv - Neuroscience 2021Quote: ... The mutations were introduced into human APP695 using the QuickChange Site-Directed Mutagenesis Kit (Stratagene, San Diego, CA, U.S.) and confirmed by sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... and RNA quality assayed using an Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit (Agilent, Santa Clara, CA). First-strand cDNA synthesis was performed using the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio ...
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bioRxiv - Cancer Biology 2020Quote: ... Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies). Individual libraries were diluted to 4nM and pooled for sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... was generated by site directed mutagenesis using primers oM18_17 and oM18_18 (Table 1) and a Quikchange II Site-directed Mutagenesis kit (Agilent, 200523). The PCR product was digested with DpnI enzyme to remove template vector and transformed into Stbl2 chemically competent cells (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... The quality of the DNA libraries was tested with the High Sensitivity DNA Bioanalysis Kit (Agilent, catalog # 5067-4626). DNA concentration of each library was measured with the Qubit dsDNA HS Assay Kit & fluorometer (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... and cDNA library size was checked using Fragment Analyzer HS NGS Fragment Kit (1-6000bp) (Agilent formerly Advanced Analytical). Only high-quality cDNA libraries without notable degradation were used for sequencing library construction ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... and quality of the isolated RNA was checked by running RNA 6000 Pico Kit using Agilent 2100 Bioanalyzer (Agilent). Paired-end ...
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bioRxiv - Cell Biology 2021Quote: ... A plasmid with smc3-K112,113R (AMp1392) was generated by site-directed mutagenesis (using QuikChange XL Site-directed mutagenesis kit, Agilent). Both plasmids were integrated into the LEU2 locus in the pCLB2-3HA-SMC3 strain background.
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bioRxiv - Microbiology 2021Quote: ... Sample quality control was performed using the Bioanalyzer High Sensitivity DNA Analysis Kit in the Agilent 2100 Bioanalyzer (Agilent). All samples were prepared and sequenced in technical replicate ...
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bioRxiv - Microbiology 2020Quote: ... Paired-end libraries (∼140-480bp insert size) were prepared using NEXTFlex DNA sequencing kit and quantified with Bioanalyzer (Agilent). WGS was performed using Illumina NextSeq 500 platform and the raw data of each strain were obtained through de-multiplexing.
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bioRxiv - Cancer Biology 2020Quote: ... Quality and quantity of the libraries were assessed with the DNA-7500 kit on a 2100 Bioanalyzer (Agilent Technologies) using a Kapa Library Quantification kit (Kapa Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was extracted from approximately 25 mg of liver tissue using the RecoverEase DNA Isolation Kit (Agilent Technologies). The λ-EG10 phage were packaged in vitro from genomic DNA using Transpack packaging extract (Agilent Technologies) ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Subsequently we strictly adhered to the Seahorse XF Cell Mito Stress Test Kit protocol (Agilent Technologies Cat# 103015-100). Cells were counted before and after the assay using a Celigo S Image cytometer (Nexcelom Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... The final sequencing libraries were inspected and quantified using the Bioanalyzer 2100 Instrument and DNA HS kit (Agilent Technologies). All libraries were diluted to 100 pM working solutions and then pooled as needed to perform the template preparation on an Ion OneTouch 2 according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... The Ribo-Zero treated RNA was quantified by using Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, CA, USA) and further used for the cDNA synthesis by using ScriptSeq™ v2 RNA-Seq kit (Epicentre ...
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bioRxiv - Microbiology 2021Quote: ... Quality of the DNA sample was checked by running Agilent High Sensitivity DNA Kit using Agilent 2100 Bioanalyzer (Agilent) before sequenced using HiSeq 2500 (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... EV small RNA size profiles were analyzed using capillary electrophoresis by RNA 6000 Pico Kit (Agilent Technologies 5067-1513) on a Fragment Analyzer (Advanced Analytical) ...
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bioRxiv - Cell Biology 2020Quote: ... The final product was assessed for its size distribution and concentration using BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... and pENTR4-HA-TRF2 (S119A/M122A) were produced by oligonucleotide-directed mutagenesis (Quick Change Site-directed Mutagenesis Kit; Stratagene) with the following mutagenic oligonucleotides and their complement.
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bioRxiv - Cell Biology 2021Quote: Yeast two-hybrid (Y2H) screens were performed using the HybriZAP two-hybrid cDNA synthesis kit (Stratagene, La Jolla, USA) as previously described (Maerker et al ...
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bioRxiv - Cell Biology 2020Quote: ... RNA quality and quantity was assessed using the Bioanalyzer RNA 6000 Nano Kit (Art. No. 5067-1511, Agilent Technologies) and Qubit™ RNA BR Assay Kit (Art ...
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bioRxiv - Cell Biology 2021Quote: ... Specific point mutations of RanGAP1 were introduced by site-directed mutagenesis with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).
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bioRxiv - Cell Biology 2021Quote: ... the pUASt-ifc was used as a backbone and site-directed mutagenesis was done with QuikChange Kit (Agilent 200523) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... The average size and concentration of all sequencing libraries was analysed using a Bioanalyser High Sensitivity DNA Kit (Agilent) followed by qPCR using SensiMix SYBR Green mastermix (Bioline ...
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bioRxiv - Biochemistry 2022Quote: PMCA1d and PMCA2w/a constructs were subjected to site-directed mutagenesis using QuikChange site-directed mutagenesis kit according to the manufacturer’s protocol (Agilent). To obtain an inactive variant D475N (the autophosphorylation site ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase activity was measured directly after mixing 10µl of cellular extract with 50 µl of Luciferase substrate (Luciferase assay kit, Stratagene). Normalization was made by measuring the β-Gal activity after 1-hour incubation of 7.5 µl of cellular extract with 50 µl of β-Gal substrate (β-Gal detection kit II ...
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bioRxiv - Microbiology 2022Quote: ... Amino acid substitutions and deletions were introduced into pET28b vectors with the QuickChange II Site-Directed mutagenesis kit (Stratagene), according to the manufacturer’s directions ...
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bioRxiv - Genomics 2022Quote: ... RNA quality was also assessed with the Agilent 2200 TapeStation system RNA high sensitivity kit (Agilent, Santa Clara, CA) before and after polyA isolation with NEBNext® Poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... and cDNA levels were measured by qPCR using the Brilliant III Ultra-Fast qPCR Kit (Agilent Technologies, Cat. #600880) and the StepOnePlusTM qPCR System (Agilent Technologies) ...
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bioRxiv - Immunology 2022Quote: ... Amber (TAG) codons were introduced site-specifically in the 2B4 TCR and CD3 plasmid using Quikchange mutagenesis kit (Agilent).
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bioRxiv - Pathology 2022Quote: ... DNA microarray analysis was performed using a Quick Amp labeling kit and a Whole Human Genome DNA Microarray 4×44K according to the manufacturer’s protocol (Agilent). Signal intensity was normalized by adjusting the data to a 75th percentile value ...
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bioRxiv - Pathology 2022Quote: ... and Fragment Analyzer quality control subsets (Fragment Analyzer Automated CE System and High Sensitivity RNA Assay Kit, Agilent Technologies), the optimal amount of total RNA (800.00ng ...
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bioRxiv - Genomics 2022Quote: ... and its integrity was assessed using RNA 6000 Nano LabChip Kit in microcapillary electrophoresis (Agilent 2100 Bioanalyzer, Agilent Technologies). Samples were then stored at −80°C until sequencing.
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bioRxiv - Genomics 2022Quote: ... and its integrity was assessed using RNA 6000 Nano LabChip Kit in microcapillary electrophoresis (Agilent 2100 Bioanalyzer, Agilent Technologies). Samples were then stored at −80°C until sequencing.
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bioRxiv - Cancer Biology 2022Quote: ... Site directed mutagenesis was performed following the manufacturer’s protocol using the Quick-Change II site-directed mutagenesis kit (Agilent Technologies ...
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bioRxiv - Biophysics 2022Quote: Site-directed mutagenesis of the human yb-1 coding gene was carried out directly on the pET22b-YB-1_1-180 expression plasmid by using the “Quikchange II XL site-directed mutagenesis kit” from Stratagene and appropriate oligonucleotides (Eurofins Genomics) ...
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bioRxiv - Biophysics 2022Quote: ... G553I) variant were cloned using the QuikChange II Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Agilent Technologies, #200523). All plasmids were sequenced to confirm the correct sequences (Genewiz).
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bioRxiv - Developmental Biology 2022Quote: ... The quality of ATAC libraries was assessed with Agilent Bioanalyzer with DNA High Sensitivity kit (Agilent Technologies, 5067-4627) and quantified with Kapa NGS library quantification kit (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... The quality of cDNA traces was checked using a High Sensitivity DNA Kit in a Bioanalyzer instrument (Agilent Technologies).
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bioRxiv - Neuroscience 2020Quote: ... Cy3 labeling was performed with T7 RNA polymerase using Agilent Low RNA Input Linear Amplification Kit following manufacturer’s instructions (Agilent). The microarray was performed using the SuperPrint G3 Mouse GE 8×60K Microarray Kit (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... The final length and concentration of the amplicons were confirmed using a Bioanalyzer DNA 1000 Kit (Agilent Technologies, USA). The library was subsequently diluted to 50 pM and loaded into the Ion 318 chip using an Ion Chef Instruments with an Ion PGM Hi-Q Chef Solutions ...
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bioRxiv - Cell Biology 2021Quote: ... RNA integrity was confirmed using the RNA Nano 6000 assay kit of the Bioanalyzer 2100 system (Agilent Technologies, USA). Poly(A ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and quality checked on a Bioanalyzer High Sensitivity Chip on an Agilent Bioanalyzer 2100 (Kit #5067-4626, Agilent Technologies). The library was sequenced on two lanes of a Illumina HiSeq 3000 system in paired end mode and 150bp read length.
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bioRxiv - Evolutionary Biology 2021Quote: ... and quality checked on a Bioanalyzer High Sensitivity Chip on an Agilent Bioanalyzer 2100 (Kit #5067-4626, Agilent Technologies). A detailed hands-on protocol can be found here ...
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bioRxiv - Developmental Biology 2020Quote: ... Final libraries were quantified and checked for fragment size distribution using a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies). Pooled sequencing of Nextera libraries was carried out using a HiSeq4000 (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Insert size and concentration of each pooled library were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies). Paired-end (2×150bp ...
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bioRxiv - Microbiology 2020Quote: ... Insert sizes and concentrations of each pooled library were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies). Paired-end sequencing (2×150-bp reads ...