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Citations for Agilent :
5151 - 5200 of 5718 citations for Rat Cystatin S CST4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... RNA samples were quantified and RNA integrity numbers (RIN) derived using the Bioanalyzer High Sensitivity RNA 6000 Pico kit (Agilent, UK).
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bioRxiv - Genomics 2024Quote: ... and the RNA integrity number (RIN) was measured using a tape-station system with an RNA screen-tape analysis kit (Agilent Technologies).
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bioRxiv - Cell Biology 2024Quote: ... The ATP production from mitochondrial respiration and glycolytic respiration in response to Oligomycin and Rotenone/Antimycin A was measured using the ATP production assay kit (Agilent, USA). The oxygen consumption rate (OCR ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified with Agencourt AMPure XP beads and sample profile was analysed with the High Sensitivity D5000 kit (Agilent Technologies) on a TapeStation (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... GHTF assessed the quality and quantity of the amplified cDNA by electrophoresis using Agilent’s Agilent High Sensitivity DNA Kit (Agilent: 5067-4626) and Bioanalyzer before library construction ...
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bioRxiv - Plant Biology 2020Quote: ... RNA purity and integrity was examined using the NanoPhotometer® spectrophotometer (IMPLEN) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). RNA concentration was measured with Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting cDNA library was assessed for yield and purity using an Agilent High Sensitivity DNA kit for Agilent 2100 Bioanalyzer (Agilent Technologies, CA). Template preparation with the cDNA libraries followed by ISP enrichment was performed using the Ion OneTouch2 system with Ion PGM™ Hi-Q™ OT2 kit (Product No ...
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bioRxiv - Cancer Biology 2021Quote: ... Library concentrations as well as fragment size distributions were also analyzed by employing the Agilent Bioanalyzer High Sensitivity DNA Kit (Agilent: 5067-4626).
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted with Norgen single cell RNA purification kit (Norgen) and the quality was checked with a Bioanalyzer 2100 (Agilent, RIN >8.0). Ribosomic RNA was depleted with Ribo-Zero™ Gold Kit (Epicentre) ...
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bioRxiv - Cell Biology 2021Quote: Measurements of aerobic respiration and glycolysis were conducted with the Seahorse Bioscience XFe-96 bioanalyzer using the Seahorse XF Cell Mito Stress Test Kit (Agilent #103015-100). Cells were seeded on XF96 cell culture microplates (Agilent #102416-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were then quantified using a Nanodrop microvolume spectrophotometer (ng/μl) and quality was assessed using the Agilent High Sensitivity DNA Kit (Agilent -5067-4626) on a Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2019Quote: Cell mitochondrial oxygen consumption rates (OCRs) were assayed in 96-well plates by using a XF Cell Mito Stress Test Kit (Cat. #: 101706100, Seahorse Bioscience, U.S.) on the XFe96 Extracellular Flux Analyzer (Seahorse Bioscience ...
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bioRxiv - Cell Biology 2020Quote: Total RNA of CFs/HVPs co-culture and conditioned CFs exposed to co-culture medium was extracted using the Absolutely RNA Miniprep Kit (Agilent Technologies, USA) according to the manufacturer’s instructions and 1 μg was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... 150 base pair oligos were chemically synthesized using proprietary Agilent technology and probes were labeled with either Cy3 or Cy5 dyes using the Genomic DNA ULS Labeling Kit (Agilent, 5190-0419). 40 ng of LAD and non-LAD probes were combined with hybridization solution (10% dextran sulfate ...
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bioRxiv - Immunology 2021Quote: ... Following assessment of the quality of total RNA using Agilent 2100 bioanalyzer and RNA Nano Chip kit (Agilent Technologies, Santa Clara, CA), rRNA was removed from 2000 ng of total RNA with Ribo-Zero rRNA removal kit specific for Human/Mouse/Rat ...
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bioRxiv - Genetics 2021Quote: ... To reproduce the A-variant the following primers were used for SDM by site directed mutagenesis using QuickChange II site directed mutagenesis kit (Agilent Technologies, UK) using the following primers ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA quality was assessed using an Agilent 2100 Bioanalyzer and the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). RNA was quantified using Qubit RNA BR Assay Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... was fragmented to 200bp using a Covaris E Series and the resultant libraries were subjected to DNA Capture using SureSelect XT Mouse All Exon kit (Agilent, Stockport, Cheshire) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The T695A and T656A mutations were generated in WT-YME1L1 plasmid via QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) and confirmed by sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored at −80°C after verifying concentration and quality by spectrophotometer (Nanodrop, Thermo scientifics) and a Bioanalyzer (RNA 6000 Nano Kit, Agilent Technologies Inc). We produced libraries for these 21 individuals using the TruSeq RNA Library Prep Kit v2 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... The mutated forms of the amplified sequence in which the potential m6A sites were replaced by cytidine (C) or guanine (G) were constructed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, 200518). The fragments were separately inserted into the pCambia2300 vector ...
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bioRxiv - Genomics 2020Quote: ... Samples were then cleaned up using Qiagen RNeasy Mini kit columns and their integrity was checked on Agilent 2200 Bioanalyzer (Agilent Technologies, USA). A total of 133 samples were selected for RNA sequencing (74 liver and 59 head kidney samples ...
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bioRxiv - Genomics 2022Quote: ... and library profiles were assessed using a DNA High Sensitivity LabChip kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was sequenced on an Illumina NovaSeq instrument (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified products were cloned using the dual-colour selection Strataclone PCR cloning kit according to the manufacturer’s protocol (Stratagene, La Jolla, USA). Up to eight colonies containing the PCR product were selected and inoculated on Luria Bertani agar slants ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... The cDNAs were then synthesized and their qualities assessed on the Agilent Bioanalyzer with High Sensitivity DNA kit (Agilent Cat# 5067-4626). cDNAs were then used for library preparation and the quality of the final libraries assessed on the Agilent Bioanalyzer with DNA 1000 kit (Agilent ...
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bioRxiv - Genomics 2020Quote: ... the fragmented product is purified and then processed to generate sequencing-ready libraries using the SureSelectXT Library Preparation kit (Agilent, CA, USA) following manufacturer instructions ...
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bioRxiv - Neuroscience 2020Quote: The point mutations and modifications in the linker sequence between CiVSD and FPs were done using the QuickCjhange II XL site-directed mutagenesis kit (Agilent Technologies, USA). The sequence verification of the novel constructs was done using Tag/dye-termination method (W ...
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bioRxiv - Genomics 2019Quote: ... Samples were cleaned up using Qiagen RNeasy Mini kit columns and their integrity was checked on Agilent 2200 Bioanalyzer (Agilent Technologies, USA). Thereafter ...
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bioRxiv - Developmental Biology 2019Quote: ... OCR and ECAR parameters were measured using the Seahorse XFp Cell Energy Phenotype Test Kit™ (Agilent Biotechnology, Cat No. 103275-100). Metabolic stress was induced by simultaneous treatment with 1μm Oligomycin and 0.125µM Carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP).
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bioRxiv - Immunology 2019Quote: Total RNA integrity was determined with the Agilent total RNA Pico Kit on a 2100 Bioanalyzer instrument (Agilent Technologies, Paolo Alto, USA). Library construction was performed with the “SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian” (TaKaRa Bio USA ...
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bioRxiv - Cell Biology 2020Quote: ... These DN proteins were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD W1758A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primers W1758A F and W1758A R.
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bioRxiv - Cell Biology 2019Quote: ... Point mutations for amino acid substitutions or truncations were generated using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA).
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bioRxiv - Genetics 2019Quote: ... Sequencing libraries then underwent a final cleanup and size selection using KAPA Pure Beads 0.55X-0.75X double-sided size selection and the average fragment size of the library was determined using the 2100 Bioanalyzer and high sensitivity DNA kit (Agilent #5067-4626). The quantity of sequenceable library for each sample was determined using the KAPA Library Quantification Kit (Roche #KK4835 ...
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bioRxiv - Neuroscience 2019Quote: ... pcDNA3.1-HA-SorCS1cβ Y1132A and GFP-tagged Rab7 T22N were generated by in vitro mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Cat #200522) from pcDNA4-SorCS1cβ-myc (kindly provided by Alan D ...
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bioRxiv - Biochemistry 2019Quote: ... and triple mutant D65K/E66K/E67K (DEE/KKK) – were generated by site-directed mutagenesis (Quick Change Lightning Site directed Mutagenesis Agilent Kit 210518) using the pLew100-TbBILBO1 plasmid and transfection of the Tb427 29.13 cell line [10] ...
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bioRxiv - Cell Biology 2019Quote: The oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) were measured using the Mito Stress Test kit (Agilent, #103010-100), following the manufactureŕs instructions ...
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bioRxiv - Genetics 2019Quote: ... The integrity of RNA was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Genomics 2019Quote: ... we only analyzed regions overlapping between RNA-seq (with more than 10x read coverage in annotated exons) and the WES enrichment kit (Agilent SureSelect 71Mb). Again ...
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bioRxiv - Biochemistry 2020Quote: The oxygen consumption rate (OCR) of LPO-exposed HLCs was measured using the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100) on a Seahorse XF Analyser (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... The mutations to generate Asp276AlaSd and Tyr435HisSd were introduced by the QuikChange II Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
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bioRxiv - Genomics 2021Quote: ... Cy3-labeled cRNA was generated from 200 ng input total RNA using Agilent’s Low Input Quick Amp Labeling Kit (Agilent Technologies, Tokyo, Japan). cDNA was generated with the primer containing a T7 polymerase promoter ...
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bioRxiv - Genomics 2021Quote: ... Each sample was prepared according to Agilent library preparation protocols (Human All Exon 50 Mb kit; Agilent Technologies, Santa Clara, CA, USA). Libraries were subjected to paired-end sequencing on an Illumina Hiseq 2000 and 2500 instrument (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Cell Biology 2020Quote: ... All mutations and the addition of the Myc-tag to the N-terminus of α-PheRS were made by following the procedure of the QuickChange® Site-Directed Mutagenesis Kit (Stratagene). The genomic α-PheRS rescue construct (Myc::α-PheRS ...
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bioRxiv - Neuroscience 2020Quote: CLC-2 mutants were generated by site-directed mutagenesis using a QuickChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX). The following table lists primers used to generate expression vectors in this study.
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... One set of single and mixed samples was stained with a FITC PNA probe and the other set was left unstained to correct for auto-fluorescence (Dako telomere kit). DNA was denatured for 10 min at 82°C and allowed to hybridize overnight ...