Labshake search
Citations for Agilent :
5001 - 5050 of 5718 citations for Rat Cystatin S CST4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Mitochondrial probes used for visualization of mt-mRNA and mt-rRNA levels were restriction fragments labeled with α-32P-dCTP and a random priming kit (Agilent). Different mitochondrial tRNAs and 7S RNA were detected using specific oligonucleotides labeled with ψ-32P-ATP ...
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bioRxiv - Physiology 2024Quote: ... Sample integrity was assessed prior to library preparation using the Bioanalyzer 2110 system with an RNA Nano 6000 Assay Kit (Agilent Technologies). One hundred fifty-base paired-end sequencing was performed using an Illumina NovaSeq platform according to manufacture instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were analyzed on Fragment Analyzer with Genomic DNA 50kb Kit and TapeStation with either High Sensitivity D5000 ScreenTape System or High Sensitivity RNA ScreenTape System (Agilent Technologies) according to the manufacturer’s protocols [41-43] ...
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bioRxiv - Genomics 2024Quote: ... The RNA quantity was assessed using the NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies) and the Agilent 2100 Bioanalyzer with the RNA 6000 Nano LabChip kit (Agilent Technologies). For DNA isolation ...
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bioRxiv - Neuroscience 2024Quote: ... and assessed for RNA integrity using an RNA Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). The sequencing libraries were then multiplexed and loaded onto the Illumina NovaSeq 6000 instrument’s flowcell ...
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bioRxiv - Neuroscience 2024Quote: ... The library molarity was determined by measuring the library size (approximately 330 bp) using the Fragment Analyzer 5300 and the Fragment Analyzer DNA HS NGS fragment kit (Agilent Technologies) and the library concentration (>2 ng/µl ...
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bioRxiv - Immunology 2024Quote: ... we first inserted the SalI site at just downstream of the termination codon of the dsRed cDNA of pIRES2-dsRed-IRF418 using a QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies), and isolated the BglII-SalI fragment containing the mIRF4-IRES-dsRed DNA region from the mutated plasmid ...
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bioRxiv - Genomics 2024Quote: ... quality control was performed by run on an Agilent 2100 Bioanalyzer System using the High Sensitivity DNA Kit (Agilent, 5067-4626). Libraries were then created from each fraction using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The quality of the RNA samples was assessed using an Agilent 2100 Bioanalyzer (RNA 6000 Nano Kit, 5067-1511, Agilent technologies) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA integrity was initially assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), and only RNA samples with an RIN value above 4 were selected for further processing ...
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bioRxiv - Genomics 2024Quote: ... DNA quality and size were assessed using an Agilent 4200 TapeStation with a gDNA ScreenTape Kit (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Microbiology 2024Quote: ... Library pools concentrations were measured using Qubit dsDNA HS Assay Kit and the quality of the libraries was assessed by High Sensitivity D5000 ScreenTape (Agilent Technologies), followed by sequencing using Illumina NextSeq500.
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from cells under the indicated experimental conditions using a Qiagen miRNAeasy kit (Valencia) and measured using an Agilent Bioanalyzer (Agilent Technologies). Illumina Novaseq 6000 libraries were prepared and sequenced by Novogene (CA ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 libraries were generated from murine tissue samples and libraries were quantified using the Agilent DNA 1000 kit (Agilent Technologies, USA).
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bioRxiv - Immunology 2023Quote: ... The predicted CLIPA8 cleavage site 63IMLR66 was replaced by IEGR [26] using the CLIPA8mutag primer and the QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies) to create plasmid pOET3-CLIPA8Xa-V5-His (Table S3) ...
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bioRxiv - Biochemistry 2023Quote: ... was used as a template to generate deletion variants for expression in insect cells using the QuickChange II XL site-directed mutagenesis kit (Agilent Technologies). For bacterial expression of the ΔN790 variant ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality control for the constructed library was performed by the Agilent Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies; Catalog # 5067-4626), while Qubit DNA HS Assay Kit was used for qualitative and quantitative analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification and quality checked of libraries was done using an Agilent 2100 Bioanalyzer instrument and a DNA 7500 kit (Agilent Technologies). Libraries were pooled and sequenced on a NovaSeq6000 S1 100 cycle run (v1.5 SBS reagents) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The quality of each library was assessed using a Bioanalyzer high sensitivity kit on an Agilent 2100 Bioanalyzer (Agilent 5067-4626). The libraries were submitted for sequencing at Cancer Research UK Cambridge Institute Genomic Core Facility using Illumina NovaSeq with 100bp single-end reads.
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bioRxiv - Cell Biology 2023Quote: The NGS sample was diluted with TE buffer and assayed using a High Sensitivity DNA Kit (cat. #5067-4626, Agilent Technologies) on a Bioanalyzer 2100 ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase was blocked using Peroxidase block (Refine Kit) followed by Protein block (X090930-2, Agilent Technologies Inc., Santa Clara, CA). Primary antibodies were applied at room temperature ...
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bioRxiv - Genetics 2023Quote: ... RNA quantity and quality were assessed using NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies) and Agilent 2100 Bioanalyser (RNA 6000 Nano LabChip kit, Agilent Technologies).
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LSD1 inhibition improves efficacy of adoptive T cell therapy by enhancing CD8+ T cell responsivenessbioRxiv - Immunology 2023Quote: Seahorse experiments were performed on sorted CD8+ cells activated in presence or absence of LSDi using XF Cell Mito Stress kit (Seahorse Bioscience). OCR and ECAR were measured with XF96 Extracellular Flux Analyzers (Seahorse Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... mutations G to C at positions 445 and 457 were introduced into DCP2 in plasmid pQZ145 (Zeidan et al. 2018) using primers AKV005/AKV006 and the Quick-change Site-Directed mutagenesis kit (Agilent, 200519), generating plasmid pAV008 containing dcp2-E149Q,E153Q ...
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bioRxiv - Molecular Biology 2023Quote: ... Promoter Capture was carried out using Hi-C libraries from three Vehicle-treated tumour samples and three Decitabine-treated tumour samples with the SureSelect target enrichment system and RNA bait library according to manufacturer’s instructions (Agilent Technologies kit), using 12 post-capture PCR cycles as required ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and for fragment size on a Fragment Analyzer using the HS NGS Fragment kit (1–6000 bp) (Agilent Technologies Inc., USA). Final enriched libraries were pooled at equimolar quantities ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The L834R mutation was introduced in EGFR using the QuikChange II Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Agilent Technologies, #200523). The plasmid used for the neural network training encoded for the extracellular and transmembrane domain (ECTM ...
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bioRxiv - Microbiology 2022Quote: ... C194 in NlpC/P60Ela and C153 in in NlpC/P60Sne were changed to alanine using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). The respective sense and antisense primers listed in Supplementary Table 1 were used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using the broad range RNA assay and rudimentary cDNA quality and size information was obtained from an Agilent 2100 Bioanalyzer with a Broad Range DNA Kit (Agilent, USA). For Qubit conversions from ug to picomoles cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA isolated from jejunum was subjected to quality control analysis using an Agilent 2100 Bioanalyzer with RNA 6000 Nano Kits (Agilent, USA). After poly A enrichment the samples were fragmented and reverse-transcribed to generate complementary DNA for sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... Required quality control steps and quantification measurements within this protocol were performed using the Agilent High Sensitivity DNA Kit (5067-4626, Agilent Technologies) and the KAPA Library Quantification Kit (2700098952 ...
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bioRxiv - Cell Biology 2023Quote: ... Probes (available in Table S7) were labeled with 32P-dCTP using the Prime-It RT random labeling kit (Agilent, catalog #300329) and hybridized to the membrane ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids harboring GlnR amino acid substitutions (pET28a-glnR derivatives) were constructed using site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit, Agilent, Inc.).DNA fragments consisting of different canonical GlnR-dependent promoters (narG whose downstream genes encoding M ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was performed in technical triplicates from at least 3 independent biological samples using the SYBR green Brilliant II fast kit (Agilent Technologies) on an Mx3005p apparatus (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The T94D mutation was introduced in the plasmids by PCR using the QuickChange Multi-Site Directed Mutagenesis kit (#200514 -Agilent Technologies). The sequence of the primer used to introduce the mutation T94D in the Nme1 sequence of plasmids is reported in key resources table ...
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bioRxiv - Microbiology 2022Quote: ... was performed to remove adapter dimers and 1 μl of the cleaned pool was visualised using the Agilent High Sensitivity DNA Kit for Bioanalyzer 2100 (Agilent Technologies) to confirm adapter-dimer removal.
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bioRxiv - Neuroscience 2023Quote: ... concentration and molarity of the libraries were then measured with the Fragment Analyzer HS NGS Fragment Kits (Agilent, cat#DNF-474). All libraries were pooled equimolar and sequenced on NovaSeq6000 and NovaSeq control software NCS v1.7 and the Illumina data analysis pipeline RTA3.4.4 and Bcl2fastq v2.20 for the following image analysis ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... and the concentrations and RNA Integrity Numbers (RIN) were determined with an RNA 6000 nano kit on a 2100 Bioanalyzer (Agilent Technologies). Libraries for TCR sequencing were prepared as described (55) ...
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bioRxiv - Neuroscience 2023Quote: ... The quality of each library was assessed by capillary electrophoresis on FragmentAnalyzer using the NGS High Sensitivity kit (Agilent, DNF-474). Sample libraries were pooled and sequenced on Illumina NovaSeq 2000 targeting 50,000 read pairs per tissue-covered spot.
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bioRxiv - Plant Biology 2023Quote: ... RNA integrity was examined using the Agilent RNA 6000 Nano kit on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and the RNA yield was determined using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Sample DNA concentrations were measured using the Qubit dsDNA HS kit and the DNA quality was evaluated using TapeStation with the Genomic ScreenTape (Agilent Technologies). Sequencing libraries were prepared using the NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and ACT1 were prepared by PCR from CLA1-2 genomic DNA using primers N1 to N10 (Supplementary Table S2d) and radioactively labeled by P32 with Random Primer Labeling Kit (Agilent, 300385). Blots were scanned using Image Quant 5.2 software (Molecular Dynamics).
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bioRxiv - Microbiology 2022Quote: 375W substitutions in all the primary isolates were introduced by site-directed mutagenesis in plasmids carrying Envs using a QuikChange site-directed mutagenesis kit (Stratagene Inc.) and mutagenic primers to introduce each mutation (56) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fragment size distributions of tagmented and dual indexed libraries were analyzed by capillary gel electrophoresis using a Bioanalyzer High Sensitivity DNA kit (50067-4626, Agilent, USA). Up to 384 high quality libraries with size distribution between 250-1000bp were pooled using equal molar quantities ...
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bioRxiv - Cell Biology 2023Quote: ... the integrity of DNAse I-treated RNA was determined using the Agilent RNA 6000 Pico Kit and an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA with RIN values > 4 was used for RT.
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bioRxiv - Cell Biology 2023Quote: ... TP53-missense mutations and protospacer adjacent motif (PAM) sequence mutation were introduced using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies). A puromycin or neomycin resistance cassette was cloned into the BamHI site which was placed between left- and right-arm of the amplified and cloned DNA fragments ...
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bioRxiv - Genomics 2023Quote: ... The concentration and quality of the sample was evaluated with Qubit dsDNA HS kit and Tapestation D5000 Screen Tape (Agilent Technologies). The expected recovery was above 50 ng.
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Bioengineering 2023Quote: ... Specific site directed mutations on the S309 and CMAB0 antibody sequence was done using the Quick-change site directed mutagenesis kit II (Agilent technologies).
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bioRxiv - Cell Biology 2023Quote: ... gene expression libraries were constructed using 1-100 ng of each amplified cDNA library and quantified using the TapeStation High Sensitivity D1000 kit (Agilent, Germany) to determine the average fragment size and library concentration ...