Labshake search
Citations for Agilent :
5051 - 5100 of 5601 citations for Rat CD178 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Samples were then cleaned up using Qiagen RNeasy Mini kit columns and their integrity was checked on Agilent 2200 Bioanalyzer (Agilent Technologies, USA). A total of 133 samples were selected for RNA sequencing (74 liver and 59 head kidney samples ...
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bioRxiv - Genomics 2022Quote: ... and library profiles were assessed using a DNA High Sensitivity LabChip kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was sequenced on an Illumina NovaSeq instrument (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified products were cloned using the dual-colour selection Strataclone PCR cloning kit according to the manufacturer’s protocol (Stratagene, La Jolla, USA). Up to eight colonies containing the PCR product were selected and inoculated on Luria Bertani agar slants ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... The cDNAs were then synthesized and their qualities assessed on the Agilent Bioanalyzer with High Sensitivity DNA kit (Agilent Cat# 5067-4626). cDNAs were then used for library preparation and the quality of the final libraries assessed on the Agilent Bioanalyzer with DNA 1000 kit (Agilent ...
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bioRxiv - Genomics 2020Quote: ... the fragmented product is purified and then processed to generate sequencing-ready libraries using the SureSelectXT Library Preparation kit (Agilent, CA, USA) following manufacturer instructions ...
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bioRxiv - Neuroscience 2020Quote: The point mutations and modifications in the linker sequence between CiVSD and FPs were done using the QuickCjhange II XL site-directed mutagenesis kit (Agilent Technologies, USA). The sequence verification of the novel constructs was done using Tag/dye-termination method (W ...
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bioRxiv - Genomics 2019Quote: ... Samples were cleaned up using Qiagen RNeasy Mini kit columns and their integrity was checked on Agilent 2200 Bioanalyzer (Agilent Technologies, USA). Thereafter ...
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bioRxiv - Developmental Biology 2019Quote: ... OCR and ECAR parameters were measured using the Seahorse XFp Cell Energy Phenotype Test Kit™ (Agilent Biotechnology, Cat No. 103275-100). Metabolic stress was induced by simultaneous treatment with 1μm Oligomycin and 0.125µM Carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP).
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bioRxiv - Immunology 2019Quote: Total RNA integrity was determined with the Agilent total RNA Pico Kit on a 2100 Bioanalyzer instrument (Agilent Technologies, Paolo Alto, USA). Library construction was performed with the “SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian” (TaKaRa Bio USA ...
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bioRxiv - Cell Biology 2020Quote: ... These DN proteins were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD W1758A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primers W1758A F and W1758A R.
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bioRxiv - Cell Biology 2019Quote: ... Point mutations for amino acid substitutions or truncations were generated using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA).
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bioRxiv - Genetics 2019Quote: ... Sequencing libraries then underwent a final cleanup and size selection using KAPA Pure Beads 0.55X-0.75X double-sided size selection and the average fragment size of the library was determined using the 2100 Bioanalyzer and high sensitivity DNA kit (Agilent #5067-4626). The quantity of sequenceable library for each sample was determined using the KAPA Library Quantification Kit (Roche #KK4835 ...
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bioRxiv - Neuroscience 2019Quote: ... pcDNA3.1-HA-SorCS1cβ Y1132A and GFP-tagged Rab7 T22N were generated by in vitro mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Cat #200522) from pcDNA4-SorCS1cβ-myc (kindly provided by Alan D ...
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bioRxiv - Biochemistry 2019Quote: ... and triple mutant D65K/E66K/E67K (DEE/KKK) – were generated by site-directed mutagenesis (Quick Change Lightning Site directed Mutagenesis Agilent Kit 210518) using the pLew100-TbBILBO1 plasmid and transfection of the Tb427 29.13 cell line [10] ...
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bioRxiv - Cell Biology 2019Quote: The oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) were measured using the Mito Stress Test kit (Agilent, #103010-100), following the manufactureŕs instructions ...
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bioRxiv - Genetics 2019Quote: ... The integrity of RNA was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Genomics 2019Quote: ... we only analyzed regions overlapping between RNA-seq (with more than 10x read coverage in annotated exons) and the WES enrichment kit (Agilent SureSelect 71Mb). Again ...
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bioRxiv - Biochemistry 2020Quote: The oxygen consumption rate (OCR) of LPO-exposed HLCs was measured using the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100) on a Seahorse XF Analyser (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... The mutations to generate Asp276AlaSd and Tyr435HisSd were introduced by the QuikChange II Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
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bioRxiv - Genomics 2021Quote: ... Cy3-labeled cRNA was generated from 200 ng input total RNA using Agilent’s Low Input Quick Amp Labeling Kit (Agilent Technologies, Tokyo, Japan). cDNA was generated with the primer containing a T7 polymerase promoter ...
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bioRxiv - Genomics 2021Quote: ... Each sample was prepared according to Agilent library preparation protocols (Human All Exon 50 Mb kit; Agilent Technologies, Santa Clara, CA, USA). Libraries were subjected to paired-end sequencing on an Illumina Hiseq 2000 and 2500 instrument (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Cell Biology 2020Quote: ... All mutations and the addition of the Myc-tag to the N-terminus of α-PheRS were made by following the procedure of the QuickChange® Site-Directed Mutagenesis Kit (Stratagene). The genomic α-PheRS rescue construct (Myc::α-PheRS ...
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bioRxiv - Neuroscience 2020Quote: CLC-2 mutants were generated by site-directed mutagenesis using a QuickChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX). The following table lists primers used to generate expression vectors in this study.
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... One set of single and mixed samples was stained with a FITC PNA probe and the other set was left unstained to correct for auto-fluorescence (Dako telomere kit). DNA was denatured for 10 min at 82°C and allowed to hybridize overnight ...
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bioRxiv - Bioengineering 2020Quote: ... RNA quality after purification was assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Pico Kit for low sample quantities (Agilent Technologies, USA) providing data on the integrity of RNA (RNA Integrity Number ...
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bioRxiv - Bioengineering 2020Quote: ... LEU4 and Ll_ilvD were generated by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). The CEN plasmids harboring wild-type ILV6 (pYZ127) ...
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Persistent DNA damage rewires lipid metabolism and promotes histone hyperacetylation via MYS-1/Tip60bioRxiv - Cell Biology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Neuroscience 2020Quote: ... The number of PCR amplification cycles was adjusted for each sample group based on the number of cycles needed to detect RNA in the lowest input sample as determined by Bioanalyzer High Sensitivity DNA Kit (Agilent #5067-4627). Negative controls for each group were run in parallel to ensure that additional cycles did not result in unspecific background product ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial respiration and glycolysis were assessed using Seahorse extracellular flux analyzer (XFe96) and Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies, USA). Cells were seeded in 96-well plates (2 × 104 cells/well) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quality control of RNA integrity and total RNA concentration was then measured using the Bioanalyzer Eukaryote Total RNA Pico kit (Agilent #5067-1513). Only samples with RIN values of 8.0 or greater were submitted for sequencing ...
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bioRxiv - Cell Biology 2021Quote: Point mutations were introduced into the wild-type human Myo1e in the pEGFP-C1 vector using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies #210519) [7 ...
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bioRxiv - Neuroscience 2020Quote: DNA isolation of FACS-enriched tdTomato cells was performed using QIAamp DNA Micro Kit (Quiagen, Germany) according to manufacturer’s instruction and checked for integrity by capillary gel electrophoresis (Bioanalyzer, Agilent Technologies, Inc., USA).
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bioRxiv - Neuroscience 2020Quote: ... the staining was revealed by 30 min incubation in a HRP-anti rabbit polymer system (DAKO REAL Envision™ Kit, #K400311-2, Agilent, France) followed by a DAB revelation of few seconds (DAKO DAB Kit ...
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... The extracted RNA was assayed for RNA integrity on 2100 Bioanalyzer using RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). RNA samples were depleted for ribosomal RNAs (rRNAs ...
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bioRxiv - Cell Biology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were then analyzed by qPCR generating CT values using NEBNEXT Library Quant Kit (#E7630) and by tape station with D1000 screen tape from Agilent (#5067-5582) to calculate fragment base pair size ...
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bioRxiv - Cell Biology 2021Quote: ... The substitution S59D and S59A were introduced into the pLVX-IRES-FLAG-tagged HERPUD1 vector using the QuikChange II XL direct-mutagenesis kit (Stratagene, cat#200522) and the mutagenesis service of GenScript (Hong Kong ...
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bioRxiv - Plant Biology 2021Quote: ... Grown colonies were screened with colony PCR using CP-F and UnivR primers and KAPA2G Robust HotStart Kit (Agilent, Supplemental Method 2). Sanger sequencing of the selected clone confirmed correct sequence of the PVY-coding part and correct in-frame insertion of GFP coding sequence ...
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bioRxiv - Plant Biology 2020Quote: ... the HY5 36th Serine (AGC) was changed into Alanine (GCC) and Aspartic Acid (GAC) respectively using Quickchange II site-directed mutagenesis kit (Agilent, Catalog #200523) and cloned into pB7FWG vectors ...
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bioRxiv - Plant Biology 2021Quote: ... needles and green stem) of 4 individual clonal Pinus radiata trees using the Plant RNA Isolation Mini Kit (Agilent, Santa Clara, CA). 50 mg of plant material from each tissue type were used for the RNA extractions ...
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bioRxiv - Microbiology 2020Quote: ... Mutations were made in the pET-51b containing the wild type DesC using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara) according to the manufacture’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA concentration and integrity were quantified using a RNA 6000 Nano Kit 2100 on a Bioanalyzer Instrument (Agilent Technologies, CA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... Deletion and mutation variants were generated using standard cloning techniques and the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, 200521).
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bioRxiv - Immunology 2020Quote: ... RNA integrity was analyzed on a subset of samples using 2100 Bioanalyzer and the RNA 6000 Nano kit (Agilent, Santa Clara, CA). Reverse transcription and cDNA synthesis was performed on 200ng of RNA using Maxima First Strand cDNA synthesis kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Sections labelled for F4 were incubated with secondary antibody from kit polymer-HRP anti-rabbit (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark) while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories ...