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Citations for Agilent :
5001 - 5050 of 5601 citations for Rat CD178 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... H80S/D83S and G93A mutants were generated by the use of QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) (60) ...
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bioRxiv - Plant Biology 2023Quote: ... Protease activity of NIa-Pro was abolished by mutating cysteine 151 to alanine (C151A) using the same construct and the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA) in both pMDC32 and pSITE (Huogen et al. ...
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was checked with an RNA Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Pathology 2024Quote: ... and the integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). 1 µg of total RNA was reverse transcribed in a 20 µL μnal reaction volume using the High-Capacity Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... The INF2-ER[K792A] GFP construct was generated via site-directed mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). All constructs were sequenced completely across their coding region.
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bioRxiv - Genetics 2024Quote: ... was used for all experiments with integrated Cas9 (Supplementary Table 3).25 Cas9D10A was generated by site-directed mutagenesis of pLS504 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent #200521) using primers MK11 and MK12 ...
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bioRxiv - Cell Biology 2024Quote: ... The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with the QuickChange II XL Site Directed Mutagenesis Kit (#200521, Agilent Technologies) with the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... The integrity and quantity of the total RNA was measured using an Agilent 2100 Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies). Total RNA obtained from each sample was subjected to sequencing library construction using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The S71A mutation was introduced into the pMALc-MBP::CDC-42 plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521, Agilent technologies) with the following primers ...
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bioRxiv - Cell Biology 2024Quote: The aforementioned mutations in the hypervariable regions of RhoA-GFP and RhoB-GFP were generated according to the manufacturing instructions of the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) using the mutagenic primers listed in the Key Resources Table ...
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bioRxiv - Cell Biology 2024Quote: Cy3-labeled cRNAs were further hybridized onto Agilent Whole Human Genome kit 4 x 44K slides (Catalog number G4112F, Agilent Technologies) containing 44,397 oligonucleotide probes in 2X Hi-RPM Hybridization buffer (Catalog number 5188-5242 ...
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bioRxiv - Developmental Biology 2024Quote: ... The quality and concentration of the libraries were assessed using the Agilent High Sensitivity DNA Kit and Bioanalyzer 2100 (Agilent Technologies) and submitted to 150 bp paired-end high throughput sequencing using Hiseq4000 (Illumina).
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bioRxiv - Biophysics 2024Quote: OCR measurements were performed with the Agilent Seahorse XF Cell Mito Stress Test kit with an XF24 extracellular flux analyser (Seahorse Bioscience). XF24 plates were coated with either 23 µl of 100% Matrigel (soft ...
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bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified RNA samples were analyzed for quality control using an Agilent 2100 Bioanalyzer and an RNA 6000 Nano kit (Agilent Technologies). Microarray experiments were performed using a custom-designed Agilent 8 × 60 K array for the E ...
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bioRxiv - Microbiology 2024Quote: ... in which the glutamic acid in the active site was substituted by alanine employing QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies) and appropriate primers listed in SI appendix ...
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bioRxiv - Immunology 2024Quote: ... Detection was achieved using Leica DAB Detection Kit (Bond Polymer Refine Detection) for CD4 and Envision FLEX DAB+ (Diaminobenzidine) (Dako Omnis) for BCL6 ...
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bioRxiv - Microbiology 2024Quote: ... To assess quality and size of the libraries samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies). Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH ...
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bioRxiv - Immunology 2024Quote: The integrity of the isolated RNA (according to the aforementioned protocol) was measured with a Fragment Analyzer using RNA Kit 15 nt (Agilent Technologies). Samples with an RNA integrity number ≥ 8 were used for sequencing ...
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bioRxiv - Neuroscience 2024Quote: The mutant PDI-Ala256 protein was prepared from the full-length wild-type human PDI cDNA using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). For protein purification ...
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bioRxiv - Microbiology 2024Quote: ... Site-directed mutagenesis of the Trp252 residue in BoGH5A was performed using the QuikChange II Site Directed Mutagenesis (SDM) Kit (Agilent Technologies) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: The ATG (+1) codon of pKL-RLBP1 was mutated to an alanine codon (GCG) using the QuikChange Site-directed Mutagenesis kit (Agilent technologies) with the F primer and its reverse complement (Table S1 ...
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bioRxiv - Zoology 2024Quote: ... RNA integrity was verified using an Agilent 2100 Bioanalyzer and an RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). RIN values ≥ 7.0 were considered appropriate for mRNA library preparation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified total RNA samples (100 ng) were labeled with Cyanine3 (Cy3) using a Low Input Quick Amp WT labeling kit (One-color; Agilent Technologies). The Cy3-labeled cRNA samples were checked for amount (>825 ng ...
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bioRxiv - Developmental Biology 2024Quote: ... HySp5-2992-mBS4:Luc and HySp5-2992-mBS5:Luc constructs were generated using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Plant Biology 2024Quote: ... thaliana cpSRP54 residues 1-564 including a point mutation at position 185 (At-cpSRP54(Q185R)) was generated using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies) using pCBi18-At-cpSRP54 as template ...
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bioRxiv - Microbiology 2024Quote: ... The size distribution of the libraries was checked by high-resolution gel electrophoresis with a Bioanalyzer instrument using the DNA 7500 Pico kit (Agilent Technologies). Libraries were quantified through fluorometry with a QubitTM fluorometer and dsDNA HS reagents (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA libraries were constructed using the TruSeq Nano DNA kit and quality-checked in the TapeStation D1000 ScreenTape System (Agilent Technologies). Genomic libraries were sequenced on an Illumina NovaSeq with PE150 (paired-end ...
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bioRxiv - Genomics 2024Quote: ... RNA samples were quantified and RNA integrity numbers (RIN) derived using the Bioanalyzer High Sensitivity RNA 6000 Pico kit (Agilent, UK).
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bioRxiv - Genomics 2024Quote: ... and the RNA integrity number (RIN) was measured using a tape-station system with an RNA screen-tape analysis kit (Agilent Technologies).
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bioRxiv - Cell Biology 2024Quote: ... The ATP production from mitochondrial respiration and glycolytic respiration in response to Oligomycin and Rotenone/Antimycin A was measured using the ATP production assay kit (Agilent, USA). The oxygen consumption rate (OCR ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified with Agencourt AMPure XP beads and sample profile was analysed with the High Sensitivity D5000 kit (Agilent Technologies) on a TapeStation (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... GHTF assessed the quality and quantity of the amplified cDNA by electrophoresis using Agilent’s Agilent High Sensitivity DNA Kit (Agilent: 5067-4626) and Bioanalyzer before library construction ...
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bioRxiv - Plant Biology 2020Quote: ... RNA purity and integrity was examined using the NanoPhotometer® spectrophotometer (IMPLEN) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). RNA concentration was measured with Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting cDNA library was assessed for yield and purity using an Agilent High Sensitivity DNA kit for Agilent 2100 Bioanalyzer (Agilent Technologies, CA). Template preparation with the cDNA libraries followed by ISP enrichment was performed using the Ion OneTouch2 system with Ion PGM™ Hi-Q™ OT2 kit (Product No ...
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bioRxiv - Cancer Biology 2021Quote: ... Library concentrations as well as fragment size distributions were also analyzed by employing the Agilent Bioanalyzer High Sensitivity DNA Kit (Agilent: 5067-4626).
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted with Norgen single cell RNA purification kit (Norgen) and the quality was checked with a Bioanalyzer 2100 (Agilent, RIN >8.0). Ribosomic RNA was depleted with Ribo-Zero™ Gold Kit (Epicentre) ...
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bioRxiv - Cell Biology 2021Quote: Measurements of aerobic respiration and glycolysis were conducted with the Seahorse Bioscience XFe-96 bioanalyzer using the Seahorse XF Cell Mito Stress Test Kit (Agilent #103015-100). Cells were seeded on XF96 cell culture microplates (Agilent #102416-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were then quantified using a Nanodrop microvolume spectrophotometer (ng/μl) and quality was assessed using the Agilent High Sensitivity DNA Kit (Agilent -5067-4626) on a Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2019Quote: Cell mitochondrial oxygen consumption rates (OCRs) were assayed in 96-well plates by using a XF Cell Mito Stress Test Kit (Cat. #: 101706100, Seahorse Bioscience, U.S.) on the XFe96 Extracellular Flux Analyzer (Seahorse Bioscience ...
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bioRxiv - Cell Biology 2020Quote: Total RNA of CFs/HVPs co-culture and conditioned CFs exposed to co-culture medium was extracted using the Absolutely RNA Miniprep Kit (Agilent Technologies, USA) according to the manufacturer’s instructions and 1 μg was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... 150 base pair oligos were chemically synthesized using proprietary Agilent technology and probes were labeled with either Cy3 or Cy5 dyes using the Genomic DNA ULS Labeling Kit (Agilent, 5190-0419). 40 ng of LAD and non-LAD probes were combined with hybridization solution (10% dextran sulfate ...
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bioRxiv - Immunology 2021Quote: ... Following assessment of the quality of total RNA using Agilent 2100 bioanalyzer and RNA Nano Chip kit (Agilent Technologies, Santa Clara, CA), rRNA was removed from 2000 ng of total RNA with Ribo-Zero rRNA removal kit specific for Human/Mouse/Rat ...
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bioRxiv - Genetics 2021Quote: ... To reproduce the A-variant the following primers were used for SDM by site directed mutagenesis using QuickChange II site directed mutagenesis kit (Agilent Technologies, UK) using the following primers ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA quality was assessed using an Agilent 2100 Bioanalyzer and the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). RNA was quantified using Qubit RNA BR Assay Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... was fragmented to 200bp using a Covaris E Series and the resultant libraries were subjected to DNA Capture using SureSelect XT Mouse All Exon kit (Agilent, Stockport, Cheshire) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The T695A and T656A mutations were generated in WT-YME1L1 plasmid via QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) and confirmed by sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored at −80°C after verifying concentration and quality by spectrophotometer (Nanodrop, Thermo scientifics) and a Bioanalyzer (RNA 6000 Nano Kit, Agilent Technologies Inc). We produced libraries for these 21 individuals using the TruSeq RNA Library Prep Kit v2 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... The mutated forms of the amplified sequence in which the potential m6A sites were replaced by cytidine (C) or guanine (G) were constructed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, 200518). The fragments were separately inserted into the pCambia2300 vector ...