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Citations for Leica :
151 - 200 of 2255 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Neuroscience 2023Quote: ... paraffin blocks were then sliced in coronal orientation at a thickness of 4-5 µm on a semiautomated rotary microtome (Leica, Germany). These sections were then collected ...
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bioRxiv - Neuroscience 2022Quote: ... cerebral organoids were fixed in 4% PFA and sectioned into 10 µm thick slices with a cryostat (CM1950, Leica). Images were captured via a Leica TCS SP8 STED confocal microscope equipped with a LAS X software ...
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bioRxiv - Neuroscience 2024Quote: ... the brains were embedded in 4% agarose/10% sucrose and sectioned at 50 µm using a vibratome (Leica VT1200S). Sections were stored at −20°C in a glycol mixture until use ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μm (Arabidopsis and barley roots) or 5 μm (barley leaves) of cryosections cut by cryostat (Leica CM1850) were attached to selected polysine adhesion slides (PolysineTM adhesion microscope slides Cat.# 48382-117 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
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bioRxiv - Biophysics 2020Quote: ... the grids were lifted and held by tweezers inside the plunge freezer chamber at 90% relative humidity and 22 °C (Leica EM-GP plunger, Leica Microsystems). 1 μL drop of X5 concentrated gold nanoparticle (150 nm ...
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bioRxiv - Neuroscience 2023Quote: ... The appropriate CNS segments were excised and frozen in optimum temperature medium (OCT; Leica FSC 22 Frozen Section Media; Leica Biosystems) to section (40 μm free-floating ...
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bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
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bioRxiv - Microbiology 2021Quote: ... CFP (Ex: 430/24 nm Chroma ET430/24x, BS: LP 455 Leica 11536022 ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were imaged within 24 hours with an SP8 Confocal Microscope (Leica), equipped with a 20x objective (NA 0.75) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Neuroscience 2021Quote: The whole-mount samples were embedded in gelatin/albumin with 4% of Glutaraldehyde and sectioned at 20 μm thickness on a vibratome (Leica, VT1000 S vibrating blade microtome). The sections were mounted in Fluoromount Aqueous Mounting Medium (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20× ...
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bioRxiv - Physiology 2021Quote: ... Undecalcified 5 μm and 10 μm thick longitudinal sections were obtained using a microtome (RM2255, Leica Biosystems., IL, USA). 5 μm sections were stained with Goldner Trichome and at least two nonconsecutive sections per sample were examined for measurement of cellular parameters ...
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bioRxiv - Neuroscience 2020Quote: ... At least 5-10 flies per genotype were scanned using the Confocal Laser Scanning Platform Leica SP8 (Leica, Germany). Samples were excited with an argon laser at 488nm (20% tube current) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The number of fluorescent and non-fluorescent worms was counted in triplicates in droplets of 5-20 µl depending on worm density using a fluorescent dissecting scope (Leica Microsystems GmbH, Wetzlar, Germany). Competitive fitness was determined as relative proportion of non-fluorescent mutant worms compared to the fluorescent reference strain CL2122.
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bioRxiv - Bioengineering 2020Quote: ... and frozen to −50°C before cryosliced with a step of 10 μm at −20 °C using a modified cryotome (CM 1950; 839 Leica Microsystems, Germany). The slices were air-dried for 2 hours before staining ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µm thick sections covering the injured area were produced using a cryostat maintained at -20°C (Leica CM 3050s, Techno-med). To assess the integrity of mitochondrial function the sections were sequentially stained for COX and SDH activities ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were sputter-coated with a 10-nm-thick gold-palladium (80/20) layer using a high vacuum sputter coater Leica EM SCD500 (Leica Microsystems GmbH) and visualised with a Hitachi S-4800 field emission scanning electron microscope (Hitachi High-Technologies Corporation ...
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bioRxiv - Cancer Biology 2024Quote: ... The brains were cryoprotected using a sucrose gradient (10%, 20%, and 30%) and then coronally sectioned (30 μm thick) using a cryostat (Leica Microsystems, Germany). Three sections around the midpoint of the hippocampus were selected per animal for each stain ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Resin blocks were stored in −20°C until trimming into 10 µm sections with a RM 2255 microtome (Leica Biosystems, Nussloch, Germany). Sections were placed on adhesive glass slides (Menzel-Gläser Superfrost® Plus ...
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bioRxiv - Cell Biology 2024Quote: ... 15 μm cryo-sections were prepared (CM1950, Leica, Germany), immersed in oil red O working solution for 5 min and rinsed with H2O ...
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bioRxiv - Plant Biology 2024Quote: ... The plants were kept at 22℃ and leaves were screened after 2 to 3 days using a confocal microscope (Leica SP8 upright).
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bioRxiv - Cell Biology 2020Quote: ... Small aliquots of about 2 μl were transferred to gold-coated copper planchettes (Engineering Office M. Wohlwend GmbH; Sennwald, Switzerland) and immobilized by high pressure freezing in a Leica HPM100 (Leica Microsystems GmbH, Wetzlar, Germany). The obtained frozen cell pellets were kept in liquid nitrogen until further use.
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bioRxiv - Cell Biology 2021Quote: ... was deposited on an EM copper grid (400-mesh, Plano G2400C, coated with 6 nm thick carbon produced with a Leica EM ACE600 sputter coater), incubated for 30-60 sec and manually blotted with filter paper ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA (10 µl of 5 µg/µl solution) was injected into each testis using glass capillaries under a stereomicroscope (M165C; Leica). Testes were held between a pair of tweezers-type electrodes (CUY21 ...
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bioRxiv - Cell Biology 2023Quote: ... objective HI PLAN I 10x/0.22 PH1, equipped with a heated stage (Tempcontrol-37; Pecon, Erbach, Germany) and stand-alone FLEXACAM C1 colour camera (Leica Microsystems, Wetzlar, Germany). Using the phase contrast images in random order ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Cell Biology 2023Quote: ... objectives N PLAN 5x/0.12 PH0 and HI PLAN I 10x/0.22 PH1, equipped with a heated stage (Tempcontrol-37; Pecon, Erbach, Germany) and stand-alone FLEXACAM C1 colour camera (Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Neuroscience 2023Quote: ... 20×0.75 NA (Leica Stellaris) oil-immersion objectives ...
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bioRxiv - Developmental Biology 2020Quote: ... tissue sections were cut at 15 μm (Leica Cryostat 1900), blocked with 10% donkey serum with 0.1% Triton X-100 in PBS (PBST ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Microbiology 2024Quote: ... sucrose in PBS overnight at 4°C and embedded in OCT compound (Agar Scientific) and cryosectioned (10 µm thick, Leica CM1850 Cryostat). Sections were dried overnight onto Thermo Scientific SuperFrost Plus Adhesion slides ...
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bioRxiv - Neuroscience 2020Quote: ... allowing for ≥24 h before slicing on a vibratome (50 µm coronal sections; Leica). The actual electrode positions were confirmed by histological DAPI staining using antifade mounting medium with DAPI (Vectashield ...
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bioRxiv - Biochemistry 2020Quote: ... for 24 hr and embedded in paraffin by an automated tissue processor (Leica, TP1020). 4 μm-thick tissue sections were obtained ...
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bioRxiv - Neuroscience 2024Quote: ... allowing for ≥ 24 h before vibratome slicing (50 mm coronal sections; Leica VT1000 S). Coronal sections were mounted with a Mowiol mounting medium (Mowiol 4-88 ...
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bioRxiv - Cell Biology 2024Quote: ... After 24 h samples were visualized on a TSC SP5 confocal microscope (Leica, #5100000750) installed on an inverted LEICA DMI 6000CS (10741320 ...
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bioRxiv - Molecular Biology 2021Quote: ... the thecal plates of the dinoflagellates were stained with the fluorescent dye Calcofluor white M2R at a final concentration of 10–20 mg mL−1 (Fritz & Triemer, 1985) and examined under epifluorescence (lamp 50W) using a Leica DM IRB (Leica Microsystemps GMbH, Wetzlar, Germany) inverted microscope ...
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bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... was equipped with a 20× objective (HC PL FLUOTAR 20×/0.50 PH2∞/0.17/D, Leica Microsystems) and with a mercury lamp (EL6000 ...
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bioRxiv - Neuroscience 2024Quote: ... 2.4 CaCl2, and 10 D-glucose bubbled with carbogen gas (95% O2, 5% CO2) using a vibratome (VT1200, Leica Biosystems, Wetzlar, Germany). Slices were allowed to recover for at least 30 min while submerged in a chamber filled with oxygenated aCSF at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were sectioned (15 μm) on a cryostat (Leica CM 1900), and sections were mounted onto Superfrost Plus microscope slides (Fisher Scientific ...