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Citations for Leica :
51 - 100 of 2255 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Neuroscience 2020Quote: ... in 10% sucrose solution and sectioned at 20 μm on a Cryostat (Leica NX70). Consecutive slides were mounted on glass slides and immunohistochemistry was performed to identify tumors or mounted on Frame Slides for microdissection (Leica ...
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bioRxiv - Neuroscience 2020Quote: ... and 10-µm-thick frozen sections were prepared at -20 °C (Leica CM1950, Germany). Sections were stored at -80 °C and used for immunofluorescence ...
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bioRxiv - Neuroscience 2022Quote: ... 10 or 20 μm-thick sections were cut using a Leica Cryostat (Leica, CM1860). Cryosections were washed with PBS to remove excess OCT on the sections and blocked in 10% Normal Donkey Serum (NDS ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 µm-thick sections were cut at -20°C using a CM3050S cryostat (Leica).
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bioRxiv - Biochemistry 2024Quote: ... The grids were glow-discharged for 20 seconds at 10 mA (Leica EM ACE200) prior to applying the sample ...
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bioRxiv - Neuroscience 2023Quote: ... and the remaining brain tissue was frozen in FSC 22 frozen section media (Leica). Brain blocks were cut coronally by a cryostat into 20 μm sections ...
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bioRxiv - Bioengineering 2022Quote: ... The gels were subsequently incubated with EM containing 10 μM forskolin (Wako) with DMSO or Inh-172 for 24 h before imaging with a Leica DMi8 (Leica Microsystems) using the Leica Application Suite X (LAS-X ...
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bioRxiv - Genomics 2020Quote: ... Sections of 5-10 μm were cut using a microtome (Leica-RM2265), placed on PEN MembranSlide glass slides with a 2.0μm membrane (Leica ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were frozen on copper tube carriers using the EM ICE high-pressure freezer (HPF, Leica). After HPF ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... followed by thin (∼3 nm) copper sputtering (Bal-tec MED 020, Leica Microsystemes SAS, Nanterre, France). Imaging was carried out using a Hitachi S800 FEG ...
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bioRxiv - Cell Biology 2023Quote: ... 0.75% glutaraldehyde) or high-pressure frozen between copper planchettes using EM ICE (Leica Microsystems, Vienna, Austria). Vitrified samples were freeze-substituted in 1% osmium tetroxide/0.1% uranyl acetate in acetone (AFS2 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Cell Biology 2024Quote: ... 4 to 5-week-old colonies were imaged using the stereo microscope (M205 FA, Leica), and the area was determined by applying thresholding to convert reflected light 8-bit images to 1-bit images and analyzed using Fiji software (ImageJ 1.54f ...
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bioRxiv - Molecular Biology 2021Quote: ... MM 24 mounting media (Leica, Wetzlar, Germany) was used to apply coverslips to slides.
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bioRxiv - Neuroscience 2021Quote: ... Cryoprotected midbrains (10%, 20%, and 30% sucrose in 0.1M PB) were cryosectioned (Leica Microsystems, Germany) at 40μm ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction products were resolved on 10-20% SDS-PAGE gels and visualized using LAS4000 (Leica), and stained with Simply Blue (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted and snap-frozen on dry ice and sectioned at -20°C in 15 µm-thick coronal slices using a cryostat (Leica CM 1860 UV) and mounted onto Colorfrost Plus glass slides (Fisher 1255017) ...
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bioRxiv - Plant Biology 2021Quote: ... five-day old seedlings were transferred to IAA containing liquid medium (10 µM) and imaged after 24 h (Leica TCS SP8 MP).
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bioRxiv - Developmental Biology 2021Quote: ... a 22×64mm coverslip (#1) was attached to a rectangular metal microscope slide frame (Leica) using magictape (Scotch) ...
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bioRxiv - Plant Biology 2021Quote: Confocal microscope (Leica TCS LSI macroscope with Plan APO 5 x and 20 x objective, Leica Microsystems) was used to detect emission of chlorophyll or redox state sensitive GFP (roGFP ...
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bioRxiv - Microbiology 2023Quote: ... copper grids were coated with thin layers of formvar and carbon (sputtered with Leica EM ACE 200), and glow discharged (PELCO easiGlow) ...
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bioRxiv - Cell Biology 2023Quote: ... 1.2 µl of the sample pellet was loaded in a type A gold coated copper carrier (Leica microsystems ...
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bioRxiv - Plant Biology 2023Quote: Nodules were embedded in 4-5% agarose and sliced in 100um sections using a vibratome (Leica). Samples were immediately imaged on slides using a 20x air objective using the excitation and emission settings described above ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were stored at −80°C until 10-15 μm coronal sections were cut using a cryostat (Leica CM3050). The sections were immediately placed on the functionalized coverslips described above ...
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bioRxiv - Biophysics 2022Quote: ... Samples were cross-sectioned into slices of 10 μm thickness by cryo-microtomy at −15°C (CM3050 S, Leica, Austria). The transversal sections were put onto microscope slides with a drop of D2O ...
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bioRxiv - Neuroscience 2020Quote: ... Tile-scan z stack images (10-15 optical sections depending on slice thickness) were collected using an SP8 confocal microscope (Leica) with a 20X magnification lens and Leica LASX software ...
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bioRxiv - Developmental Biology 2024Quote: ... as previously described.15 Embedded tissues were sectioned at 10 μm thickness on a HistoCore MULTICUT semi-automated rotary microtome (Leica) onto SuperfrostPlus glass slides (Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... Sections of 10 µm at −20 ° C were performed in cryostat (Leica CM1860UV, Leica Biosystems, Germany). The first section was stained with toluidine blue for visual inspection of the presence of islets ...
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bioRxiv - Biochemistry 2023Quote: ... Sections of 10 µm at −20 ° C were performed in cryostat (Leica CM1860UV, Leica Biosystems, Germany). The first section was stained with toluidine blue for visual inspection of the presence of islets ...
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bioRxiv - Cell Biology 2024Quote: ... Frozen blocks were then cut into 10 μm or 20 μm sections on a Cryostat (Leica) and mounted on Superfrost Plus slides (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... Embryos were then recorded on an inverted spinning disk confocal microscope (Leica DMI4000B-CSU 22 Yokogawa) with a 100X immersion objective (HCX PL APO 1.4 oil ...
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bioRxiv - Physiology 2023Quote: ... The samples were then rinsed in PBS and cryopreserved in FSC 22 Frozen Section Media (Leica) and stored at −80°C until use ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.1M phosphate buffered saline (PBS, Fisher #BP399-20) at +4°C before slicing on a freezing microtome (Leica) in a 1:12 series of 40 µm thick coronal sections ...
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bioRxiv - Immunology 2023Quote: ... Extracted VLPs were deposited on a carbon-coated copper grid and prepared using an EM GP workstation (Leica). Vitrified VLPs were prepared on a Lacey Carbon TEM grid (copper ...
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bioRxiv - Cell Biology 2024Quote: 70 nm ultrathin sections were prepared onto Formvar-coated copper slot grids using an ultramicrotome (EM UC7, Leica). The sections were post-stained for 5 min in 5% uranyl acetate in water at 60°C and followed by 5 min in lead citrate ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were sectioned from 5-10 μm thick on a Leica CM1950 cryostat (Leica) and these cryosections were attached to Superfrost Plus Gold slides (ThermoFischer Scientific).
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bioRxiv - Plant Biology 2024Quote: ... after which 5–10 mm transverse sections were prepared using a microtome (Leica RM2265).
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bioRxiv - Biophysics 2024Quote: ... After blotting for 4-5 s in a Leica EM GP2 plunge freezer (Leica Microsystems, Wetzlar, Germany) with the chamber set to 5°C and 95% humidity ...
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bioRxiv - Neuroscience 2024Quote: Mice were sacrificed by cervical dislocation and sagittal slices (250-400 μm) were prepared with an angle of 10-15° to maintain rostrocaudal striatonigral projections 82 using a vibrating microtome (Leica VT1200). Slice preparation was performed in ice-cold sucrose-based artificial cerebrospinal fluid (ASCF ...
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bioRxiv - Neuroscience 2022Quote: ... samples on SEM stubs were coated with a 10 or 15 nm platinum or gold layer using the sputter coating machine EM ACE600 (Leica Microsystems) at 30 mA current to be finally placed into the Crossbeam 540 FIB-SEM (Carl Zeiss Microscopy GmbH ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... frozen and sectioned at a thickness of 10 µm at -20 °C using a cryostat (Leica, CM1800). Sections were placed on Superfrost Microscope glass slides (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... and cryo-sectioned at a thickness of 10 µm at −20°C on a cryostat (Leica, CM1850). The sections were placed on a coated glass slide (MAS-GP type A ...
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bioRxiv - Zoology 2023Quote: ... Paraffin blocks were sectioned (5-7 μm, 20-40 sections per block) using a microtome (Leica RM 2125RT, Germany). Histological sections were stained with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2024Quote: ... 20 mM choline bicarbonate) saturated with carbogen (5% CO2 and 95% O2) using a vibratome (Leica VT 1200 S). 250 μm brain slices were incubated in 31°C ACSF solution (119 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... Adult nota were mounted in 22 0.8% low melting agarose and imaged on an MZ16 stereomicroscope (Leica) with a QImaging MicroPublisher 6 Color Camera ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Cell Biology 2020Quote: ... Sections mounted on Pioloform filmed copper grids prior to staining with 2% aqueous uranyl acetate and lead citrate (Leica). Ultrathin sections were examined on a Phillips CM 100 Compustage (FEI ...
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bioRxiv - Microbiology 2024Quote: Copper tubes were trimmed at <-140°C in an EM UC7 Cryo-ultramicrotome equipped with a micromanipulator (Leica Microsystems). Ultrathin sections were produced with a 35° diamond cryo- knife (Diatome ...