Labshake search
Citations for Leica :
101 - 150 of 2255 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The samples were sectioned to 100 nm-thick onto bare 100-mesh copper grids by ultramicrotome (Leica EM UC7) for further observation ...
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bioRxiv - Biochemistry 2024Quote: ... were glow-discharged (15 sec at 15 mA) on a Leica EM ACE600 instrument (Leica Microsystems). 5 µl of UPF2L or UPF2L / RNA complex was applied to the grids at a concentration of 0.01 to 0.005 mg/ml and incubated for 1 min before blotting with Whatman filter paper (GE Healthcare) ...
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bioRxiv - Physiology 2024Quote: ... apparatus The tissues were embedded in paraffin (Roth) sliced in 4-5 μm sections using a microtome (Leica). The sections underwent deparaffination and rehydration procedures ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were paraffin-embedded and cut into 4–5 μm sections using a microtome (RM2125, Leica; Germany). These sections were then stained with hematoxylin and eosin (H&E ...
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bioRxiv - Physiology 2021Quote: ... Muscle sections (10 µm) were cut from the mid belly at -20° C on a cryostat (Leica, CM1950), collected on SuperFrost Plus (VWR ...
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bioRxiv - Physiology 2024Quote: ... Muscle sections (10 µm) were cut from the mid belly at -20°C on a cryostat (Leica, CM1950), collected on SuperFrost Plus (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... 10 μm-thick (to minimize cell overlap) coronal sections were collected at -20 °C using a cryostat (Leica; brains were adjusted to the temperature of the cryostat for 30 min prior to sectioning) ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post fixed in 4% paraformaldehyde overnight and cryoprotected in 20% sucrose azide prior to freezing and sectioning at 40μm (CM3050S; Leica, Wetzlar, Germany). Tissue slices containing M2 were stained with neutral red and electrode placement was validated ...
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bioRxiv - Developmental Biology 2024Quote: ... at room temperature, incubated with 20% sucrose (Fisher BioReagents, BP220-1) overnight at 4°C and then embedded in OCT (Leica, 14020108926) and stored at −80°C until further use.
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bioRxiv - Developmental Biology 2024Quote: ... the samples were transferred to 30% Sucrose solution overnight at 4°C before OCT (Sakura) inclusion for performing 20 µm coronal sections using Cryostat (Leica Biosystems). Cryostat sections were washed three times with PBST (1x PBS with Tween 20 ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were stretched with chloroform to eliminate compression and mounted on Pioloform filmed copper grids prior to staining with 1% aqueous uranyl acetate and lead citrate (Leica). They were viewed on a Philips CM100 Compustage Transmission Electron Microscope with images collected using an AMT CCD camera (Deben) ...
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bioRxiv - Cell Biology 2020Quote: ... Ultrathin sections (50nm) were deposited onto formvar-coated copper grids (Plano) and postcontrasted using 1% uranyl acetate in water and ultrostain (Leica). TEM images were acquired on a JEM 1400plus (JEOL ...
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bioRxiv - Biochemistry 2022Quote: ... were transferred to formvar-coated copper grids and poststained with uranyl acetate and lead citrate in an EM-Stain apparatus (Leica) and subsequently observed with a Zeiss EM 900 transmission electron microscope (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... All the samples were sectioned at 5–10 μm thickness using a microtome (Leica microsystem, Germany). Haematoxylin and eosin (H&E ...
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bioRxiv - Immunology 2024Quote: ... 5 to 10-minute-long videos were recorded using an inverted Leica SP8 microscope (Leica Microsystems). Image analysis was performed with Fiji.
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bioRxiv - Physiology 2024Quote: ... Blocks were cut into thick (10 μm) serial (3-4) sections and placed onto PEN-slides (Leica) along with thin (4 μm ...
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bioRxiv - Neuroscience 2024Quote: Fresh-frozen brains were cut into 10 µm thick coronal sections at −15°C using a Leica CM 1860 UV cryostat (Leica Biosystems, Nussloch, Germany). Tissue sections were thaw-mounted onto conductive MALDI IntelliSlides™ (Part No 1868957 ...
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bioRxiv - Bioengineering 2024Quote: ... Japan) and cut into 10 or 15 μm-thick frozen sections on cryofilm using a cryostat (CM3050S or CM1860; Leica Microsystems, Wetzlar, Germany). After cutting out the frozen sections ...
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bioRxiv - Plant Biology 2022Quote: ... Stained seed coats were fixed with 4-5% agarose gel and dissected by microtome (Leica, VT1000 S, Wetzlar, Germany) and were visualized by Nikon 80i-epi-microscope ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5; Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Microbiology 2020Quote: ... UK) which had been glow discharged for 20 seconds at 10 mA in an Ace 200 (Leica Microsystems (UK) Ltd ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Microbiology 2024Quote: ... at 17°C and 63.5 bar and the sample was sputter coated with a 5 nm thick layer of Au/Pd (80/20) using the Leica ACE600 (Leica Microsystems). Samples were mapped by SEM using an Aquilos 2 dual-beam cryo-focused ion beam-scanning electron microscope (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... a z-stack (1 µm sections) was acquired at 21–22 °C with the HC PL APO 63×/1.40 NA oil immersion objective (Leica).
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bioRxiv - Neuroscience 2020Quote: ... brains were transferred into 30 % sucrose in phosphate buffer until they sank down before they were cut into 40 µm- or 60 µm- thick coronal sections using a dry-ice-cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at −20 °C in cryoprotectant solution (25 % ethyleneglycol ...
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bioRxiv - Neuroscience 2022Quote: ... brains were cut into 40 μm coronal sections using a dry ice–cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at 4°C in cryoprotectant solution (25% ethylene glycol and 25% glycerol in 0.1 M phosphate buffer).
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bioRxiv - Neuroscience 2022Quote: ... incubated in 30 % sucrose in phosphate buffer for two days and cut into 40 μm coronal sections using a dry-ice-cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at 4 °C in cryoprotectant solution (25 % ethyleneglycol ...
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bioRxiv - Neuroscience 2024Quote: ... brains were cut into 40 µm coronal sections using a dry ice–cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at 4°C in cryoprotectant solution (25% ethylene glycol and 25% glycerol in 0.1 M phosphate buffer) ...
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bioRxiv - Bioengineering 2024Quote: ... Then the specimens were sectioned to 150 nm-thick onto bare 100-mesh copper grids by an ultramicrotome (Leica EM UC7) with cryo-chamber to maintain the samples under -150 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 20 and 20 µm thickness respectively using a cryostat (Leica). Immunohistochemistry on tissue sections will be performed according to standard procedures ...
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bioRxiv - Neuroscience 2023Quote: ... frozen tissue blocks from each of the 4 AD subjects were sectioned at 10 micron thickness (Leica, CM3050s) and stored at −80°C ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were coated with platinum (4 to 10 nm) in a EM MED020 vacuum coating system (Leica) and observed in a scanning electron microscope (ESEM Quanta 250 FEG FEI ...
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bioRxiv - Zoology 2021Quote: ... embedded in paraffin and sectioned at 5–10 μm thickness on a Leica microtome RM2255 (Leica, Germany). Sections were de-waxed ...
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bioRxiv - Physiology 2021Quote: ... tissue was fixed in 4% buffered paraformaldehyde and samples subsequently paraffin-embedded and 5 μm sections prepared on a microtome (Leica). Adipose sections were viewed at 20 x magnification ...
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bioRxiv - Plant Biology 2020Quote: Seventy µm-thick transversal sections of the midrib of leaflets 4 or 5 from L1 leaf were cut using a vibratome (Leica) and stained with periodic acid (1% w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
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bioRxiv - Immunology 2021Quote: Kidneys were isolated from resting mice after PBS perfusion and fixed O/N in 20 mL of 10% Neutral Buffered Formalin (Leica). Fixed kidneys were paraffin-embedded using a Shandon Citadel 1000 tissue processor (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Cells were imaged at 24 h using a M205FA Stereomicroscope (Leica) and a DMi8 inverted microscope (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... 24 and 48 (MCF7) using a Leica Dmi8 microscope (Leica Microsystems), and wound size was observed over time ...
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bioRxiv - Neuroscience 2021Quote: ... S1 were dissected and incubated in 0.1M PBS containing 30 % sucrose until the tissue sank to the bottom before being embedded and frozen in Surgipath FSC 22 Clear Frozen Section Compound (Leica) freezing medium on dry ice ...
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bioRxiv - Cancer Biology 2022Quote: ... brain tissues were extracted and snap frozen using liquid nitrogen before being implanted in FSC 22 Frozen Section Media (Leica). Using a cryostat (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... tissue samples were transferred into gold or copper carriers and frozen under high pressure (>300 bar) using an HPM010 (Leica, Wetzlar, Germany). Frozen samples were stored in liquid nitrogen until further processing ...
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bioRxiv - Cell Biology 2020Quote: ... Sections (15 μm-thick, Leica CM 1950 Cryostat) were obtained ...
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bioRxiv - Neuroscience 2021Quote: ... sectioned (15 μm) using a cryostat (Leica, Germany) and stored at − 80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the proximal and distal 2-3 mm of LCCA were discarded and the remaining portion (4-5 mm) were embedded in optimal cutting temperature compound for frozen sectioning on a cryostatmicrotome (Leica Biosystems). Frozen sections (10 μm thick ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 with 5% CO2/ 95% O2) and whole brain sagittal slices (300 µm) were cut using a vibratome (VT1200, Leica Biosystems). Afterwards slices were transferred to 34°C cutting solution for 45 minutes to recover and finally maintained at RT until used for recordings.