Labshake search
Citations for Leica :
1151 - 1200 of 1264 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... before being embedded in 5% low-melting agarose and sectioned into 100-400 μm-thick slices using a Vibratome (Leica, VT1200). Slices were blocked and permeabilized using PBST supplemented with 5% donkey serum and 0.3% Triton X at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated for 5-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached ∼0 mV (8 ...
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bioRxiv - Molecular Biology 2024Quote: ... were acquired with a Leica SP5 laser scanning confocal microscope or with Leica Stellaris 5 confocal microscope and LAS AF Lite software (Leica Microsystems). The images were analysed with ImageJ.
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Microfluidic Formation of Ultrathin, Handleable Collagen Sheets Exhibiting Toe-heel Tensile BehaviorbioRxiv - Bioengineering 2024Quote: The morphology of collagen sheets was imaged under wet condition were using a light sheet/confocal microscope (model Leica Stellaris 5), equipped with a water immersion lens (40× magnification ...
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bioRxiv - Cancer Biology 2024Quote: ... Processed tissues were embedded in paraffin and sectioned at room temperature using a manual rotary microtome (5 um sections; Leica RM2235). Frozen tongues were sectioned using a cryostat (10 um sections ...
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bioRxiv - Developmental Biology 2024Quote: ... Long bone samples were embedded in paraffin after dehydration and 5-7 μm-thick paraffin sections were cut with a microtome (RM2255, Leica Biosystems). Sections were stained with von Kossa ...
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bioRxiv - Developmental Biology 2024Quote: ... The ovaries were dissected in 5% glycerol (Carl Roth) and immediately analyzed using a Leica DMI6000b epifluorescence inverted microscope (Leica Microsystems), equipped with GFP ...
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bioRxiv - Developmental Biology 2024Quote: ... Stacks with a z-space of 0.4 μm were captured with a confocal microscope (Stellaris 5, Leica Microsystems GmbH, Wetzlar, Germany). The previous segmentation model trained on Hoechst labelled images was refined in Cellpose to create models for nuclei and GM130 labeled images.42 Digital masks of nuclei and Golgi bodies were created with Cellpose ...
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bioRxiv - Neuroscience 2024Quote: ... NeuN and active caspase-3 signals in dLGN (Figure S4C) were imaged using a 20x/0.7 NA water objective (Leica HC PL APO 20x/0,7 IMM CORR CS2). A 590 nm laser and an emission read of 595 nm – 750 nm were used for imaging caspase-3 and a 499 nm laser with a 509 nm - 595 nm emission read was used for the NeuN signal ...
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bioRxiv - Cell Biology 2020Quote: ... neural crest populations were captured at 4 days post-fertilation (dpf) using a Leica Sp8 and analyzed with the Leica Application Suite X (Leica Microsystems, Buffalo Grove, IL) software for image capturing ...
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bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290, Leica Microsystems GmbH, Wetzlar, Germany) with a mounted digital camera ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were head-fixed in a 1.5 x 4-inch polycarbonate tube under a custom-built fluorescence macroscope consisting of back-to-back 50 mm objective lens (Leica, 0.63x and 1x magnification), separated by a 495nm dichroic mirror (Semrock Inc ...
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bioRxiv - Molecular Biology 2020Quote: Frozen coronal ovine hypothalamic blocks (n=4 per group) for in-situ hybridization were cut into 16µm sections using a cryostat (CM3050s Leica Microsystems, Ltd., Milton Keynes, UK), and thaw mounted onto poly-l-lysine coated slides (VWR International ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Neuroscience 2022Quote: ... embedded them in 4% w/v agar/PBS and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were mounted with Fluorsave (Table 1S) and stored in the dark at 4°C until imaging on a Leica Stellaris5 confocal microscope (Leica Microsystems CMS GmbH, Germany). Quantification was performed as previously described42 ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
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bioRxiv - Plant Biology 2024Quote: ... 4 DAT seedlings were treated with 1 M PEP1 for 24h before they were imaged using confocal miscrscopes (Leica TCS SP8 and Zeiss LSM700). To visualize the GFP protein ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
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bioRxiv - Neuroscience 2024Quote: ... They were then perfused transcardially with ice-cold (4°C) phosphate buffered saline (PBS) using the Perfusion Two™ system (Leica Biosystems, Chicago, IL).
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bioRxiv - Neuroscience 2024Quote: ... following imaging and either underwent cardiac perfusion with phosphate buffered saline and 4% paraformaldehyde or had their brains extracted and post-fixed in 4% paraformaldehyde before being cut on a vibratome in 40 µm sections (Leica VT1000S, Deer Park, IL). Sections through the olfactory bulb were mounted on slides and were coverslipped using Fluoromount-G containing DAPI (SouthernBiotech ...
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bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).
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bioRxiv - Developmental Biology 2020Quote: Pregnant female mice (E3.5 and E9.5) were sacrificed by cervical dislocation for embryo dissections under a dissecting microscope (M165C or M80, Leica Microsystems; Wetzlar, Germany) as previously described (Behringer et al ...
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bioRxiv - Immunology 2021Quote: ... Fixed tissue samples were then embedded in paraffin and 5 µm thick sections were cut at a rotary microtome (Leica, Wetzlar, Germany). After a 10 min incubation in PBS ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: we measured the teleochrysalid abdomen surface area (Troscianko, 2014) from high-resolution photos (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD).
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were allowed to rest in cold oxygenated (95% O2/5% CO2) aCSF and then sliced coronally using a vibratome (Leica VT1000 S). Brain slices (400-μm thick sections ...
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bioRxiv - Genetics 2022Quote: ... H&E staining was performed on 5 µm sections from paraffin-embedded retinas according to standard protocols for light microscopy and photomicrography (Leica, Wetzlar, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Cells were then imaged at 37°C in a chamber supplemented with humidified 5% CO2 through white light laser confocal microscope TCS SP8 (Leica, Wetzlar, Germany). Movie S1 was processed by Bitplane-Imaris 9.5 (Andor Technology ...
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bioRxiv - Biophysics 2020Quote: ... stage of growth were imaged every 10 minutes for 12 hours in a customized incubator (37°C, 5% CO2, and 95% humidity) on a confocal microscope (Leica, TCL SP8), with acquisition of fluorescent and bright-field image z-stacks (Fig ...
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bioRxiv - Bioengineering 2021Quote: ... The samples washed in PBS (3x 5 min) and embedded in Mowiol prior to confocal laser scanning microscope analysis (Leica TCS SP8X). Collagen fiber organization was determined as described in the supplementary information (supplementary Fig.S2-4 and supplementary Eq.S1.).
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bioRxiv - Immunology 2021Quote: ... Lung tissue was embedded in Optimal Cutting Temperature Compound (Tissue-Tek) and 5 μm sections were cut on CM1950 Cyrostat (Leica, Weltzlar, Germany). Sections were blocked with 1% Rat serum and 1% FcBlock in PBS for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were collected onto cover glasses and coated with gold with sputter current 30 mA for 5 seconds (Leica EM ACE200). Cover glasses were sealed on aluminum stabs using carbon tape ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm each of carbon and iridium (Leica ACE 600). Prepared samples were imaged on a Zeiss Merlin FE-SEM using an InLens detector at 1.2 – 1.5 kV and 400 – 500 pA depending on the sample ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were cut at 30 µm and BAT sections were cut at 5 µm using a cryostat (Leica Biosystems, Nussloch, Germany). Brain sections were labelled with Anti-FLAG M2 F1804 ...
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bioRxiv - Plant Biology 2023Quote: ... Stems were rehydrated first in ethanol 50% and later in water and then embedded in agarose 5% before being transversally cut using a vibratome (Leica vt 1000s) to obtain 120 micron thickness sections stored in water ...
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bioRxiv - Neuroscience 2022Quote: ... Delineation of CA1.SR and MDG subregions were performed using light microscope under 5 × objective lens, the newCAST software (Visiopharm, Hørsholm, Denmark) modified for stereology with a digital camera (Leica DFC 295, Germany) and a motorized stage (Ludl Mac 5000 ...
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bioRxiv - Plant Biology 2022Quote: ... The abaxial side of the leaf sample was imaged using a 63× objective (Leica, HC PL APO CS2 5 63×/1.20 Water). GFP was excited with a 488 nm WLL laser and collected at 503-530 nm ...
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bioRxiv - Developmental Biology 2023Quote: The images were initially processed in Fiji with a customed macro to batch open the lif files (Image taken on Leica Stellaris 5) and split the images into individual channels ...
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bioRxiv - Microbiology 2022Quote: ... cells were imaged using a Stedycon optical setup (Abberior Instruments GmbH) integrated with Leica SP 5 microscope (Leica Microsystems GmbH, Manheim, Germany).
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bioRxiv - Neuroscience 2022Quote: ... Fixed brains were washed three times for 5 min with PB and 150 µm-thick coronal sections were cut with a vibratome (Leica Microsystems, VT1000S). Tissue sections were labeled with DiIC18 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal and para-sagittal slices (300 μm) were prepared in ice-cold oxygenated (95% O2 / 5% CO2) ACSF with a vibratome (LeicaVT1000S, Leica Biosystems, Germany), then warmed to 36°C for 45 mins ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... the nuclei were stained with DAPI (1:2000) and the images were captured with 63X oil immersion objective by the confocal microscope (Leica STELLARIS 5). The measurements related to cell shape analysis of the nuclei in apical ...
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bioRxiv - Developmental Biology 2024Quote: ... PFA-fixed and paraffin embedded tissue slides (5 µm) were incubated for 1h at 60°C with Bond Dewax solution (Leica Bond, AR9222) to deparaffinize tissue sections ...
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bioRxiv - Biophysics 2024Quote: ... alveolospheres were cultured in 35 mm petri dish in a customized incubator (5% CO2, 37°C, 95% humidity) on a confocal microscope (Leica, TCL SP8). A 25 × /0.95 numerical aperture water objective and a 63×/1.20 numerical aperture water objective were both used for imaging ...
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bioRxiv - Neuroscience 2024Quote: ... 2.4 CaCl2, and 10 D-glucose bubbled with carbogen gas (95% O2, 5% CO2) using a vibratome (VT1200, Leica Biosystems, Wetzlar, Germany). Slices were allowed to recover for at least 30 min while submerged in a chamber filled with oxygenated aCSF at room temperature ...