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Citations for Leica :
1051 - 1100 of 1264 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and sectioned to a thickness of 7 µm for the testis and 5 µm for the epididymis using an Ultrathin Microtome from Leica, Germany ...
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bioRxiv - Plant Biology 2024Quote: ... The protein patterns of AP2:VENUS and SOC1:GFP under continuous LDs at the SAM were acquired with a Stellaris 5 confocal microscope (Leica) for fluorescence quantification (Fig ...
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bioRxiv - Immunology 2024Quote: The quality of cells and grids was assessed in widefield mode on a Stellaris-5 cryo-confocal laser scanning microscope equipped with a cryo-stage and x50/0.9 numerical aperture objective (Leica Microsystems). Z-stacks of desired regions were acquired in confocal-scan mode with a 547 nm laser line (560-580 nm emission) ...
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bioRxiv - Neuroscience 2024Quote: Positioning and fluorescence of VLPs/cells on grids was assessed in widefield mode on a Stellaris-5 cryo-confocal laser scanning microscope equipped with a cryo-stage and x50/0.9 numerical aperture objective (Leica Microsystems). Z-stacks of desired regions were acquired in confocal-scan mode with a 490 nm laser (505-540 nm emission) ...
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bioRxiv - Immunology 2024Quote: ... Sections were paraffin embedded by the histological core at the RI-MUHC and cut into 5 µm section using a microtome (Leica). Sections were deparaffinized in sequential xylenes followed by serial hydration steps in descending concentrations of ethanol ...
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bioRxiv - Microbiology 2024Quote: ... 3-3.5 μL) were applied to glow-discharged lacey carbon grids and plunge frozen using an EM GP Plunge Freezer (Leica). Grids were positively glow-discharged using amylamine for the Hyper2 sample ...
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bioRxiv - Immunology 2024Quote: ... 5 µm formalin fixed paraffin-embedded sections were stained with Akoya Biosciences Opal multiplex automation kit reagents (Leica Cat#ARD1001EA) on a Leice BondRX autostainer ...
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bioRxiv - Neuroscience 2024Quote: ... Brains was postfixed overnight at 4 °C in a 4 % formaldehyde solution and then embedded in 5 % agarose gel for sectioning (50-µm-thick coronal sections) with a vibratome (VT1000S, Leica).
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bioRxiv - Neuroscience 2024Quote: ... at 5 µm-thickness for paraffin samples or in a cryostat at 30 µm-thickness for frozen samples (Leica, Germany).
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bioRxiv - Biophysics 2024Quote: ... Grids were blotted for 5 seconds then plunge frozen into liquid ethane cooled by liquid nitrogen using an EM GP2 (Leica) plunge freezer at 4°C and 100% relative humidity ...
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bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence signal was recorded between 410 nm and 600 nm after excitation at 405 nM in 5 nm increments using a confocal microscope (Leica TCS SP8-AOBS ...
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bioRxiv - Cell Biology 2024Quote: ... samples were freeze-substituted at -90°C in anhydrous acetone containing 2% osmium tetroxide and 0.1% uranyl acetate at -90°C for 72 hours and warmed to room temperature with a gradient of +5°C per hour (EM AFS-2, Leica). Sections of the samples were analysed in a Tecnai Spirit T12 Electron microscope equipped with an Eagle OCD camera (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... filaments with three or more cells of 5-day-old protonemata regenerated from protoplasts were imaged using a wide-field microscope (DMI4000B, Leica). The length of subapical cells was analyzed using Fiji software (ImageJ 1.54f ...
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bioRxiv - Cancer Biology 2024Quote: IHC of MPO was performed using 5-micron paraffin embedded sections on a Leica Bond RX auto stainer (Leica Biosystems). MPO primary antibody was diluted to 1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Biochemistry 2024Quote: ... and finally incubated with 3 μM MitoSOX Red for 10 min and fixed with paraformaldehyde (2%) and analyzed by fluorescence microscopy (Leica TCS SP5, Buffalo Grove, IL).
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were dissected and post-fixated overnight in 4% paraformaldehyde, followed by sectioning (coronal plane, 50 μm) with a vibratome (VT100S, Leica Biosystems, Wetzlar, Germany). The slices were collected and stored in PB for immunohistochemistry.
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Cell Biology 2021Quote: ... samples were mounted on aluminum SEM stubs and sputter-coated with a 4 nm platinum-palladium layer using a Leica EM ACE600 sputter coater (Leica Microsystems, Illinois, USA). Images were acquired at 2 kV using a Zeiss Sigma 300 SEM (Zeiss ...
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bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Pathology 2022Quote: ... in situ hybridization was performed on 12 µm-thick sections of 4% PFA-fixed OCT mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval (HIER ...
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bioRxiv - Bioengineering 2022Quote: ... Perfusion devices were then fixed with 4% para-formaldehyde and imaged using a 16x immersion lens on SP8 multiphoton microscope (Leica Microsystems, Morrisville, NC). Tumor volumes were quantified by imaging individual tumors post bioprinting
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bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then embedded in a 4% agarose/1x PBS w/v solution prior to mounting and slicing on a vibratome (Leica VT 1000 S). 40 μm or 60 μm sections containing the pPVN (−0.70 to −1.06 mm relative to Bregma) ...
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bioRxiv - Pathology 2022Quote: ... Pictures of 4-6 different fields per sample were taken under a Leica DM5000 B microscope equipped with a digital CCD (Leica Camera, Wetzlar, Germany).
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bioRxiv - Physiology 2021Quote: ... Fluorescence emission was detected every 526 ms using a Leica SP8 DIVE multiphoton confocal fluorescence imaging system as described above (emission at 460-520 nm for Fluo-4 and at 580-640 nm for Fura Red) (Leica Microsystems, Heidelberg, Germany).
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bioRxiv - Immunology 2023Quote: ... Formalin-fixed paraffin-embedded (FFPE) sections of 4 μm thickness were stained with antibodies specific for CD21 (predilute, clone 2G9, Leica Biosystems, Danvers, MA), CD3 (predilute ...
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bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The fluorescence of the dyes was visualized and recorded 4 hours after the application using a White Light Laser Confocal Microscope (Leica TCS SP8 X). WT ...
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bioRxiv - Plant Biology 2023Quote: ... The fluorescence of the dyes was visualized and recorded 4 hours after the application using a White Light Laser Confocal Microscope (Leica TCS SP8 X).
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bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
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bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were taken and fixed in 4% PFA at RT overnight and 50 μm slices were cut with a vibratome (Leica VT 1000 S). For brain sections from the anterograde and trans-synaptic tracing studies ...
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bioRxiv - Neuroscience 2023Quote: ... for 48 hours at 4°C before being cut into 30 μm thick coronal serial sections on a sliding microtome (Leica Biosystems, Wetzlar Germany). The free-floating brain sections were then stored in antifreeze solution at −20°C until immunohistochemical staining.
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Cell Biology 2022Quote: ... spheroids were fixed with 4% PFA at room temperature and photographed using a Leica DM IL LED microscope (Leica Microsystems GmbH, Wetzlar, Germany). Contact inhibition ...
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bioRxiv - Immunology 2024Quote: ... Brains were placed in 4 °C s-ACSF and sectioned with a vibrotome at 350–400 μm thick (Leica VT1200S; Leica, Nusslock, Germany). Collected slices were then incubated at 32 °C for 1 h in i-ACSF (124 mM/L NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were stored at 4°C and imaged using the 63x oil immersion objective on the Leica TCS SP5 confocal microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were sputter-coated with a 4 nm platinum layer using a Leica EM ACE600 sputter coater (Leica Microsystems GmbH, Wetzlar, Germany) and examined using a Zeiss Crossbeam 340 Field-Emission Electron Microscope (Zeiss Microscopy ...
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bioRxiv - Cancer Biology 2024Quote: ... The immunohistochemistry protocol is briefly described as follows: tissue sections (4 μm) were stained in a Leica BOND RX automated stainer (Leica Biosystems Nussloch GmbH). The tissue sections were deparaffinized and rehydrated following the Leica BOND protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Molecular Biology 2021Quote: ... The surface of the cocoon and all the fibers were observed using scanning electron microscopy (SEM, Hitachi, Japan) at an acceleration voltage of 5 kV and a polarizing optical microscope (Leica, Germany). For transmission electron microscope (TEM ...
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bioRxiv - Developmental Biology 2021Quote: ... The entire cerebellum of each animal (N=5 analyzed) was sectioned coronally at 50μm thickness on a vibratome (Leica VT 1000S) and all sections were processed for immunohistochemistry using a human nuclear antigen antibody (HuNu ...