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Citations for Leica :
851 - 900 of 1264 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Neuroscience 2024Quote: ... the sucrose solution was removed and organoids were placed on OCT (at least 3 organoids on each OCT drop) and cryosectioned using a standard cryostat (Leica CM1860, Leica Biosystems). 20 μm thick sections were collected on Ultra Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then embedded in low melting point agarose (σ Type VIIA, 3% in Ames medium) and 250 µm slices were obtained using a vibratome (Leica Microsystems, VT1200S) in cold (∼4° C) ...
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bioRxiv - Neuroscience 2024Quote: Fresh-frozen brain tissue samples from the three groups (n=3) were sectioned at 10 μm thickness using a cryostat (Leica Instruments GmbH, Germany). Tissue optimization was performed with TO slides (part no ...
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bioRxiv - Cell Biology 2024Quote: ... Samples of GN2 or scraped cells were placed between two 3 mm diameter aluminum- or gold-coated (Type A) specimen carriers with 100 µm indentations (Leica Microsystems Inc., Austria). HT29 cells directly grown on the same type of 3 mm diameter aluminum- or gold-coated (Type A ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... when embryos were fixed by 4% PFA for ensuing WISH analysis or imaged using a Fluorescent stereoscopic microscope (LEICA M205 FCA). 10 pg of gsc mRNA and 10 pg of ndr2 mRNA were injected ...
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bioRxiv - Biochemistry 2020Quote: ... but the purified V2R-Gs-Nb35 complex was at a concentration of 4 mg/ml and the cryo-EM grids were prepared using a EM GP2 (Leica microsystems) plunge freezer with a 4s blotting time (100% humidity and 4°C).
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Molecular Biology 2021Quote: ... The paraffin blocks transferred to room temperature and the blocks sections cut 4 mm thick in a rotatory microtome (Leica RM2135) and mounted in glass slides ...
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bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were removed and post-fixed in PBS containing 4% paraformaldehyde over night before cutting 50μm thick coronal sections on a vibratome (VT 1000 S from Leica, Wetzlar, Germany). Floating sections were permeabilized with 2% Triton X-100 in PBS overnight and blocked with 2% normal goat serum (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in 4% low-melting agarose and coronal cortical slices were generated (300 µm) with a vibrating microtome (VTS1000 Leica Microsystems). Slices were next incubated for 60 minutes at 37°C in DMEM/F-12 imaging media without phenol red supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Neuroscience 2020Quote: Brains were embedded in agar (3-4% w/v dissolved in dH2O) before being cut into 50 μm coronal sections on a vibrating microtome (VT1000S; Leica Microsystems). Free-floating tissue sections were collected in series ...
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bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 300μm thickness were embedded into 4% low melting agarose and further cut into 50 μm slices with vibrating-blade microtome (Leica, Germany). Nuclei were counterstained by adding a small drop of ProLong™ Gold Antifade Mountant with DAPI (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: Brain organoids (4.5 months old) were embedded into 4% low melting agarose and 300 μm slices were cut with vibrating-blade microtome (Leica, Germany). Slices were prepared using ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were embedded in paraffin and 4 μm thick sections were made using a Leica RM2165 microtome (Leica, Wetzlar, Germany). Alcian Blue/Periodic Acid-Schiff (AB/PAS ...
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bioRxiv - Cell Biology 2022Quote: ... The imaged area was marked with a circle (4 mm in diameter) around the center of the imaged area using an objective diamond scriber (Leica, 11505059)39 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted from the skull and stored in 4% formaldehyde before being cut in sagittal sections (50 μm) using a vibratome (Leica VT1000) and stained with cresyl violet ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were cultured for 4 hours at 37 °C in the climate chamber of a Leica SP8 Confocal system (Leica Microsystems) microscope ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Plant Biology 2020Quote: ... by infiltration for 10 min in a vacuum and after further incubation for 4 h processed using the Leica Semi-Enclosed Benchtop Tissue Processor TP1020 (Leica Biosystems) for an ethanol series (75 % ...
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bioRxiv - Bioengineering 2021Quote: ... according to standard histological procedures and 4 μm thick sections of the paraffin blocks were cut on the microtome (Leica, HM325). The paraffin sections were stained with Haematoxylin-Eosin (HE ...
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bioRxiv - Neuroscience 2021Quote: ... brains of perfused mice (n=4 per group) were serially cut into 35 μm-thick coronal sections using cryostat (Leica, Germany). Sections were permeabilized in PBS containing 0.3% Triton X-100 for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Brains electroporated with the pCAG-mRFP;Hes5p-GFP plasmid were dissected 11 h after electroporation and embedded in 4% Agarose in complete HBSS medium (Polleux and Ghosh, 2002) and sectioned using a vibratome (Leica VT1200S). Electroporated areas of the lateral cortex were microdissected under the fluorescent dissecting scope (Zeiss Axio Zoom) ...
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bioRxiv - Immunology 2022Quote: ... grids were metalized by platinum sputtering (4 nm) using a Leica ACE600 cryo-sputter coater equipped with a cryo-stage (Leica Microsystems). Metallized samples were then transferred to a pre-cooled Crossbeam 550 FIB-SEM microscope (Carl Zeiss NTS GmbH ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post fixed in 4% paraformaldehyde overnight and cryoprotected in 20% sucrose azide prior to freezing and sectioning at 40μm (CM3050S; Leica, Wetzlar, Germany). Tissue slices containing M2 were stained with neutral red and electrode placement was validated ...
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bioRxiv - Neuroscience 2022Quote: ... The lumbar region of spinal cord was embedded in low-melting point agarose (4% solution, in PBS) and sectioned into 40 µm thick coronal sections with a vibrating microtome (Leica VT1000S). For KCC2 staining ...
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bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused with 4% PFA in PBS and brains were removed and sectioned coronally or sagittally on vibratome (Leica VT1000) at 50-75 μm and mounted on slides with ProLong(tm ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde in PBS and imaged using laser scanning confocal microscopy (LEICA DMi8, Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed with 4% PFA in PB overnight and 50-µm coronal sections of the brainstem were obtained on a vibratome (Leica VT1200S). Expression of EGFP at the calyx of Held terminals was visualized using an epifluorescence inverted microscope (CKX41 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...