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Citations for Leica :
951 - 1000 of 1217 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...
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bioRxiv - Cancer Biology 2023Quote: ... The standard ACD Red Rev BOND Rx protocol was modified to include BOND Epitope Retrieval Solution 2 (Leica Biosystems) pretreatment for 25min at 95°C and to increase Amp5 step time to 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... outside of the necrotic region) (Figure 8-figure supplement 1E-F) from 2 sections with the Application Suite Core v2.4 software (Leica).
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bioRxiv - Physiology 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 95 °C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was immersed in 30% sucrose for 2 days followed by embedding in Tissue-Tek OCT and sectioning of 50μm slices in a cryostat (Leica). Staining was done as described (Licht et al. ...
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bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
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bioRxiv - Microbiology 2023Quote: ... Slides were imaged on an inverted Leica TCS SP5 confocal microscope using a 63x/1.40 oil objective with 2-3x digital zoom at calibrated magnifications and recorded with LAS AF software (Leica). Quantitation of dextran and LAMP-1 colocalization with bacteria was analyzed with CellProfiler 3.1.9 (27 ...
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bioRxiv - Cell Biology 2022Quote: ... fluorescence images were captured every 2 s for 6 min using fluorescence microscopy (#AF6000-DMI6B; Leica Microsystems, Wetzlar, Germany) (objective lens ...
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bioRxiv - Biochemistry 2022Quote: ... Plunging was performed at 90-100% humidity via automatic backside blotting (via Leica Microsystems Cryo GP 2 plunge freezer) or dual-side blotting (Mark IV FEI/Thermo Fisher Scientific Vitrobot) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and cut into coronal slices (thickness 300 µm, -1.5 mm to 2 mm relative to bregma) on a vibratome (Leica VT1200S ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed for 20 min with Bond Epitope Retrieval Solution #2 (Leica Biosystems, equivalent EDTA, pH 9.0). Primary antibody (dilution at 1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Developmental Biology 2020Quote: Pregnant female mice (E3.5 and E9.5) were sacrificed by cervical dislocation for embryo dissections under a dissecting microscope (M165C or M80, Leica Microsystems; Wetzlar, Germany) as previously described (Behringer et al ...
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bioRxiv - Immunology 2021Quote: ... Fixed tissue samples were then embedded in paraffin and 5 µm thick sections were cut at a rotary microtome (Leica, Wetzlar, Germany). After a 10 min incubation in PBS ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: we measured the teleochrysalid abdomen surface area (Troscianko, 2014) from high-resolution photos (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD).
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were allowed to rest in cold oxygenated (95% O2/5% CO2) aCSF and then sliced coronally using a vibratome (Leica VT1000 S). Brain slices (400-μm thick sections ...
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bioRxiv - Genetics 2022Quote: ... H&E staining was performed on 5 µm sections from paraffin-embedded retinas according to standard protocols for light microscopy and photomicrography (Leica, Wetzlar, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Cells were then imaged at 37°C in a chamber supplemented with humidified 5% CO2 through white light laser confocal microscope TCS SP8 (Leica, Wetzlar, Germany). Movie S1 was processed by Bitplane-Imaris 9.5 (Andor Technology ...
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bioRxiv - Biophysics 2020Quote: ... stage of growth were imaged every 10 minutes for 12 hours in a customized incubator (37°C, 5% CO2, and 95% humidity) on a confocal microscope (Leica, TCL SP8), with acquisition of fluorescent and bright-field image z-stacks (Fig ...
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bioRxiv - Bioengineering 2021Quote: ... The samples washed in PBS (3x 5 min) and embedded in Mowiol prior to confocal laser scanning microscope analysis (Leica TCS SP8X). Collagen fiber organization was determined as described in the supplementary information (supplementary Fig.S2-4 and supplementary Eq.S1.).
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bioRxiv - Immunology 2021Quote: ... Lung tissue was embedded in Optimal Cutting Temperature Compound (Tissue-Tek) and 5 μm sections were cut on CM1950 Cyrostat (Leica, Weltzlar, Germany). Sections were blocked with 1% Rat serum and 1% FcBlock in PBS for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were collected onto cover glasses and coated with gold with sputter current 30 mA for 5 seconds (Leica EM ACE200). Cover glasses were sealed on aluminum stabs using carbon tape ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm each of carbon and iridium (Leica ACE 600). Prepared samples were imaged on a Zeiss Merlin FE-SEM using an InLens detector at 1.2 – 1.5 kV and 400 – 500 pA depending on the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... then cryoprotected with 20% sucrose in PBS for 24 hours at 4°C and cut in 5-µm-consecutive thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picro Sirius Red Stain Kit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were cut at 30 µm and BAT sections were cut at 5 µm using a cryostat (Leica Biosystems, Nussloch, Germany). Brain sections were labelled with Anti-FLAG M2 F1804 ...
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bioRxiv - Plant Biology 2023Quote: ... Stems were rehydrated first in ethanol 50% and later in water and then embedded in agarose 5% before being transversally cut using a vibratome (Leica vt 1000s) to obtain 120 micron thickness sections stored in water ...
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bioRxiv - Neuroscience 2022Quote: ... Delineation of CA1.SR and MDG subregions were performed using light microscope under 5 × objective lens, the newCAST software (Visiopharm, Hørsholm, Denmark) modified for stereology with a digital camera (Leica DFC 295, Germany) and a motorized stage (Ludl Mac 5000 ...
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bioRxiv - Plant Biology 2022Quote: ... The abaxial side of the leaf sample was imaged using a 63× objective (Leica, HC PL APO CS2 5 63×/1.20 Water). GFP was excited with a 488 nm WLL laser and collected at 503-530 nm ...
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bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
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bioRxiv - Developmental Biology 2023Quote: The images were initially processed in Fiji with a customed macro to batch open the lif files (Image taken on Leica Stellaris 5) and split the images into individual channels ...
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bioRxiv - Microbiology 2022Quote: ... cells were imaged using a Stedycon optical setup (Abberior Instruments GmbH) integrated with Leica SP 5 microscope (Leica Microsystems GmbH, Manheim, Germany).
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bioRxiv - Neuroscience 2022Quote: ... Fixed brains were washed three times for 5 min with PB and 150 µm-thick coronal sections were cut with a vibratome (Leica Microsystems, VT1000S). Tissue sections were labeled with DiIC18 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal and para-sagittal slices (300 μm) were prepared in ice-cold oxygenated (95% O2 / 5% CO2) ACSF with a vibratome (LeicaVT1000S, Leica Biosystems, Germany), then warmed to 36°C for 45 mins ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... the nuclei were stained with DAPI (1:2000) and the images were captured with 63X oil immersion objective by the confocal microscope (Leica STELLARIS 5). The measurements related to cell shape analysis of the nuclei in apical ...
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bioRxiv - Developmental Biology 2024Quote: ... PFA-fixed and paraffin embedded tissue slides (5 µm) were incubated for 1h at 60°C with Bond Dewax solution (Leica Bond, AR9222) to deparaffinize tissue sections ...
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bioRxiv - Biophysics 2024Quote: ... alveolospheres were cultured in 35 mm petri dish in a customized incubator (5% CO2, 37°C, 95% humidity) on a confocal microscope (Leica, TCL SP8). A 25 × /0.95 numerical aperture water objective and a 63×/1.20 numerical aperture water objective were both used for imaging ...
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bioRxiv - Neuroscience 2024Quote: ... 2.4 CaCl2, and 10 D-glucose bubbled with carbogen gas (95% O2, 5% CO2) using a vibratome (VT1200, Leica Biosystems, Wetzlar, Germany). Slices were allowed to recover for at least 30 min while submerged in a chamber filled with oxygenated aCSF at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Z-stack images with a spacing of 5 µm were obtained using an epifluorescence microscope with an automatic scanning stage (DMi8 S; Leica, Wetzlar, Germany) equipped with a ×10 dry objective (Leica ...
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bioRxiv - Biophysics 2020Quote: ... S cerevisiae (Sc) cells strain #2 after ~5h of starvation were high pressure frozen (HPM010, AbraFluid) and freeze substituted (EM-AFS2, Leica) with 0.1% uranyl acetate in acetone for 55h at −90°C ...
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bioRxiv - Biochemistry 2022Quote: ... Neutrophils were then counted at the site of transection at 2 hours post injury (hpi) using a fluorescence dissecting stereomicroscope (Leica).
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Plant Biology 2021Quote: ... The cross section of 2-4μm was cut with a glass knife using the ultramicrotome Leica EM UC7 (Leica Microsystems). The semi-thin sections were stained for 30mins in 0.1% aniline blue in 0.1M K3PO4 at RT ...
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bioRxiv - Plant Biology 2021Quote: ... The stained sections were observed within 1hr to detect callose deposition using confocal laser scanning microscopy (TCS SP5 2; Leica).
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.