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Citations for Leica :
801 - 850 of 1217 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The resulting samples were cut to 5 µm by using a Reichert-Jung 820H Histostat rotary microtome (Leica, Wetzlar, Germany) and Drukker VC186200 diamond knives (Cuijk ...
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bioRxiv - Neuroscience 2020Quote: ... brains were embedded in a 5% low-gelling agarose gel (in PBS) and 50µm coronal slices were cut using a vibroslicer (Leica VT1000S). Slices were then stored in PBS-Azide (0.02-0.05% ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5 ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA (10 µl of 5 µg/µl solution) was injected into each testis using glass capillaries under a stereomicroscope (M165C; Leica). Testes were held between a pair of tweezers-type electrodes (CUY21 ...
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bioRxiv - Neuroscience 2022Quote: ... Near-horizontal middle entorhinal cortical (bregma –6.5 mm to –5.1 mm) slices of thickness of 350 μm were obtained using a vibrating blade microtome (Leica Vibratome) while the brain was submerged in oxygenated ice-cold cutting solution ...
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bioRxiv - Physiology 2021Quote: ... tissue was fixed in 4% buffered paraformaldehyde and samples subsequently paraffin-embedded and 5 μm sections prepared on a microtome (Leica). Adipose sections were viewed at 20 x magnification ...
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bioRxiv - Plant Biology 2020Quote: Seventy µm-thick transversal sections of the midrib of leaflets 4 or 5 from L1 leaf were cut using a vibratome (Leica) and stained with periodic acid (1% w/v ...
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bioRxiv - Microbiology 2021Quote: ... imaging the colony and crystal formation at regular intervals at 5× magnification with a Leica MZ7.5 stereomicroscope (Leica, United Kingdom), using an Infinity 2 Lumenera microscope camera (Teledyne Technologies Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μm sections were taken for H&E staining using standard methods and imaged using a DMI 6000B microscope (Leica). To examine tdTomato expression in sections ...
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bioRxiv - Cancer Biology 2022Quote: Hematoxylin and Eosin (H&E) staining was performed on 5 μm thick slices using an automated system (Leica Autostainer, ST5010) with the protocol as described in Supplemental Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Retinal cups were embedded in 5% agar moulds and immediately underwent sectioning on a fully automated vibratome (Leica VT1200 S). Sections were ‘Chased’ by incubation with 0.6 µM SNAP-Cell 647-SiR (New England Biolabs (S9102S ...
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bioRxiv - Neuroscience 2022Quote: ... Horizontal slices containing the substantia nigra were cut at 222 μm in Krebs buffer bubbled with 95/5% O2/CO2 using a vibrating microtome (Leica). Slices were allowed to recover at 30°C in vials with 95/5% O2/CO2 Krebs with MK801 (10 μM ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000 ...
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bioRxiv - Bioengineering 2022Quote: PTFE surfaces were sputter-coated with platinum to develop a 5 nm thick conductive layer on the surface using an EM ACE600 sputter coater (Leica). Images were acquired using a Quanta 450 scanning electron microscope (FEI company).
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells expressing OMM-Cepia3 and ER-mit RspA-splitFAST were mounted into an open-topped chamber in mKRB supplemented with TFCoral (5 µM) and imaged by a Thunder Imager 3D Cell Culture (Leica), equipped with a LED8 illumination system ...
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bioRxiv - Plant Biology 2023Quote: ... cell walls were labelled with propidium iodide (5 µg/mL) and imaged using a 20x PLAN APO multi-immersion objective (Leica), with λexc = 488 nm and λem = 551-651 nm ...
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bioRxiv - Plant Biology 2024Quote: ... The cells were then washed 3 times for 5 min with PBS and covered with PBS before imaging them with a TCS SP8 confocal laser-scanning microscope (Leica). Excitation/emission settings were 488 nm/498-524 nm HyD1 SMD hybrid detector for CAH4 ...
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bioRxiv - Neuroscience 2024Quote: ... 87 mM NaCl and 25 mM D-glucose) gassed with 95% O2/5% CO2 to give a pH of 7.4 using the VT-1200 Vibratome (Leica, Germany). Slices were then allowed to recover for at least an hour in a chamber filled with ACSF ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin-embedded tissue was sectioned into approximate 0.5cm3 cubes and then cut into 5-μm slices by a LEICARM2235 Slice Machine (Leica, Germany). The samples were stained with haematoxylin/eosin (H/E ...
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bioRxiv - Pathology 2023Quote: ... 5 μm thick sections were taken from the paraffin blocks using a rotary microtome (Leica RM2155, Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin-eosin (HE) ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue visualization and image collection was performed using a Leica Stellaris 5 Confocal microscope and LAS X software (Leica Microsystems). All images were acquired using 40X (HC PL APO CS2 ...
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bioRxiv - Biophysics 2024Quote: ... were evenly positioned on a ring installed 5 cm above the sample positioned at the focal plane of the objective lens (OBJ; 100×/1.44, Leica, Germany), as shown in Fig ...
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bioRxiv - Biochemistry 2022Quote: ... serial 5 μm thick sections of adult scallop’s eye were obtained using a Leica CM1950 freezing microtome (Leica, Wetzlar, Germany) for Raman spectroscopy testing (alpha300R ...
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bioRxiv - Immunology 2023Quote: ... with a stage top incubator to maintain 37 °C and 5% CO2 or on a Leica TCS SP8 Laser Scanning Confocal (Leica) fluorescence microscope with an incubator to maintain 37 °C and 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
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bioRxiv - Cell Biology 2023Quote: ... the tibiae were embedded in methyl methacrylate (MMA) and 5 µm sections cut with a tungsten steel knife on a motorised rotary microtome (Leica). Sections were stained either with Goldner’s Trichrome to analyse osteoid and lipid droplets or counterstained with calcein blue to quantify the amount of bone formed between the injected alazarin red-S and calcein ...
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bioRxiv - Bioengineering 2023Quote: ... Immunostained cells and tissues were imaged on the Evos Revolve widefield microscope using a 20x objective or Stellaris 5 confocal microscope (Leica) with a 10x or 25x water-immersion objective to capture data in DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were imaged either with a Zeiss Observer A1 microscope and the AxioVision software 4.8 or with a STELLARIS 5 confocal microscope (Leica, Germany) at 40x (HC PL APO 40x/1.30 OIL CS2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 μm or 0.5 μm step sizes was performed on a Leica TCS sp8 Lightning Confocal/STED microscope coupled to LasX software (Leica) using a 63x (1.4 ...
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bioRxiv - Biophysics 2023Quote: ... A volume of 3ul of the sample was applied onto glow-discharged (Leica EM ACE200, 30 s at 5 mA) grids (Quantifoil R1.2/1.3 200 mesh Cu grids ...
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bioRxiv - Developmental Biology 2023Quote: ... and 18.5 counting images were acquired using Leica Application Suite X (LAS X) on a Leica DMI8 fluorescent microscope from Leica Microscopy ...
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bioRxiv - Neuroscience 2023Quote: ... and immobilized in 5% methyl cellulose for imaging with a M165 FC stereo microscope and LAS Software V4.21 (Leica Microsystems). Total body length measurements and eye area were quantified using Fiji/ImageJ software (63) ...
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bioRxiv - Developmental Biology 2023Quote: ... ganglionic eminences explant cultures were placed in a humidified and thermoregulated chamber maintained at 37 °C and 5% CO2 on the stage of a spinning-disk microscope (Leica), equipped with an Orca Flash 4.0 Camera ...
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bioRxiv - Neuroscience 2024Quote: ... Brains was postfixed overnight at 4 °C in a 4 % formaldehyde solution and then embedded in 5 % agarose gel for sectioning (50-µm-thick coronal sections) with a vibratome (VT1000S, Leica).
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bioRxiv - Neuroscience 2024Quote: ... at 5 µm-thickness for paraffin samples or in a cryostat at 30 µm-thickness for frozen samples (Leica, Germany).
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bioRxiv - Developmental Biology 2024Quote: ... and sectioned to a thickness of 7 µm for the testis and 5 µm for the epididymis using an Ultrathin Microtome from Leica, Germany ...
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bioRxiv - Plant Biology 2024Quote: ... The protein patterns of AP2:VENUS and SOC1:GFP under continuous LDs at the SAM were acquired with a Stellaris 5 confocal microscope (Leica) for fluorescence quantification (Fig ...
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bioRxiv - Immunology 2024Quote: The quality of cells and grids was assessed in widefield mode on a Stellaris-5 cryo-confocal laser scanning microscope equipped with a cryo-stage and x50/0.9 numerical aperture objective (Leica Microsystems). Z-stacks of desired regions were acquired in confocal-scan mode with a 547 nm laser line (560-580 nm emission) ...
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bioRxiv - Microbiology 2024Quote: ... 3-3.5 μL) were applied to glow-discharged lacey carbon grids and plunge frozen using an EM GP Plunge Freezer (Leica). Grids were positively glow-discharged using amylamine for the Hyper2 sample ...
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bioRxiv - Immunology 2024Quote: ... Sections were paraffin embedded by the histological core at the RI-MUHC and cut into 5 µm section using a microtome (Leica). Sections were deparaffinized in sequential xylenes followed by serial hydration steps in descending concentrations of ethanol ...
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bioRxiv - Neuroscience 2024Quote: Positioning and fluorescence of VLPs/cells on grids was assessed in widefield mode on a Stellaris-5 cryo-confocal laser scanning microscope equipped with a cryo-stage and x50/0.9 numerical aperture objective (Leica Microsystems). Z-stacks of desired regions were acquired in confocal-scan mode with a 490 nm laser (505-540 nm emission) ...
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bioRxiv - Immunology 2024Quote: ... 5 µm formalin fixed paraffin-embedded sections were stained with Akoya Biosciences Opal multiplex automation kit reagents (Leica Cat#ARD1001EA) on a Leice BondRX autostainer ...
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bioRxiv - Biophysics 2024Quote: ... Grids were blotted for 5 seconds then plunge frozen into liquid ethane cooled by liquid nitrogen using an EM GP2 (Leica) plunge freezer at 4°C and 100% relative humidity ...
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bioRxiv - Cancer Biology 2024Quote: IHC of MPO was performed using 5-micron paraffin embedded sections on a Leica Bond RX auto stainer (Leica Biosystems). MPO primary antibody was diluted to 1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... filaments with three or more cells of 5-day-old protonemata regenerated from protoplasts were imaged using a wide-field microscope (DMI4000B, Leica). The length of subapical cells was analyzed using Fiji software (ImageJ 1.54f ...
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bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence signal was recorded between 410 nm and 600 nm after excitation at 405 nM in 5 nm increments using a confocal microscope (Leica TCS SP8-AOBS ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Cell Biology 2020Quote: ... 0.05% uranyl acetate in acetone and then placed in a AFS-2 freeze substitution machine (Leica Microsystems, Vienna). Samples were freeze substituted at −90°C for 72 h ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...