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Citations for Leica :
851 - 900 of 1217 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then cut into sections of 2 μm thickness with an ultra-microtome (Leica EM UC6). Sections were placed on glass coverslips for immunohistochemistry.
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal cerebellum sections 300 μm thick were cut with a vibratome (VT1200S, Leica or 7000smz-2, Campden Instruments) in ice-cold high-sucrose ACSF ...
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bioRxiv - Neuroscience 2020Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
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bioRxiv - Immunology 2022Quote: ... formalin-fixed paraffin-embedded tissue sections were pre-treated with the BOND Epitope Retrieval Solution 2 (Leica Biosystem) at 100°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Plant Biology 2021Quote: ... 2’,7’-dichlorofluorescein (DCF) fluorescence was visualized by confocal laser scanning microscopy (CLSM) (Leica TCS-SP5, Mannheim, Germany) with excitation/emission at 495 nm/525 nm.
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bioRxiv - Microbiology 2020Quote: ... 0.05 % uranyl acetate in acetone and placed in an AFS-2 freeze-substitution machine (Leica Microsystems, Wetzlar, Germany). Tissues were freeze-substituted at −90 °C for 72 h ...
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bioRxiv - Pathology 2021Quote: ... These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each ...
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bioRxiv - Cell Biology 2020Quote: ... in dry methanol for 32 hours at −90° C in an AFS 2 freeze substitution unit (Leica Biosystems). The temperature was then raised 5° C per hour to −45° C ...
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bioRxiv - Microbiology 2020Quote: ... samples were de-stained with 50% Lactophenol for 1–2 hours before observation under a light microscope (Leica Microscope ...
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bioRxiv - Microbiology 2022Quote: ... with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265 ...
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bioRxiv - Physiology 2024Quote: ... and Mac-2-staining were imaged using a Leica DMi8 Inverted Fluorescent Microscope (Leica Microsystems, Buffalo Grove, IL). Histological quantifications were performed using Image J software (v1.52q ...
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bioRxiv - Cancer Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-treated in ER2 (epitope retrieval solution 2) antigen retrieval buffer for 20 min at 93 °C (Leica), blocked in antibody (Ab ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Cell Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Microbiology 2023Quote: ... was applied to glow-discharged Quantifoil R2/2 grids and vitrified with a GP2 plunge-freezing device (Leica).
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bioRxiv - Biophysics 2023Quote: ... FFPE tissue samples were cut using a ultramicrotome in 2 πm thick sections (RM2125 RTS, Leica Biosystems, Germany).
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bioRxiv - Plant Biology 2023Quote: Roots fixed with 2% (v/v) formaldehyde in Tris were embedded in Cryo-Gel (Leica Biosystems, ID:39475237) and cut onto a cryostat (Leica CM1950 ...
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bioRxiv - Neuroscience 2024Quote: ... Horizontal slices (300µm) of the OB were cut using a vibratome (Campden Instruments 7000SMZ-2 or Leica VT1000S) and stored in ACSF containing the following (in mM) ...
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bioRxiv - Microbiology 2024Quote: ... with a tissue pretreatment of 30 minutes at 100°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems) and 1:750 dilution of OPAL 570 (AKOYA ...
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bioRxiv - Molecular Biology 2021Quote: ... The surface of the cocoon and all the fibers were observed using scanning electron microscopy (SEM, Hitachi, Japan) at an acceleration voltage of 5 kV and a polarizing optical microscope (Leica, Germany). For transmission electron microscope (TEM ...
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bioRxiv - Developmental Biology 2021Quote: ... The entire cerebellum of each animal (N=5 analyzed) was sectioned coronally at 50μm thickness on a vibratome (Leica VT 1000S) and all sections were processed for immunohistochemistry using a human nuclear antigen antibody (HuNu ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... Detached nodules were embedded in 5% low melting point agarose and sliced into 30-μm thick sections with a rotary microtome RM2235 (Leica Microsystems). Roots were also stained with X-Gal (Affymetrix ...
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bioRxiv - Neuroscience 2020Quote: CGEs from E14.5 Plxna4+/+;Htr3a-GFP or PlxnA4-/-;Htr3a-GFP coronal acute brain slices were microdissected under a fluorescence dissecting scope (Leica M165FC) and cells dissociated with 0.25% trypsin-EDTA (Life Technologies) ...
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bioRxiv - Bioengineering 2020Quote: ... The diffusion experiments were performed in the environmentally controlled chamber (set at 37 °C, 5% CO2) of a fluorescent microscope (Leica, CTR6500). The dextran solution was perfused inside the microvessel channel and images were acquired every 30 sec over 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were rapidly removed from the skull and coronal brain slices containing the hippocampal formation (350μm thick) were prepared in the ice-cold cutting solution bubbled with 95%O2/5% CO2 using Vibratome VT1200S (Leica Microsystems) and then incubated in an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Cell Biology 2021Quote: ... the proximal and distal 2-3 mm of LCCA were discarded and the remaining portion (4-5 mm) were embedded in optimal cutting temperature compound for frozen sectioning on a cryostatmicrotome (Leica Biosystems). Frozen sections (10 μm thick ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 with 5% CO2/ 95% O2) and whole brain sagittal slices (300 µm) were cut using a vibratome (VT1200, Leica Biosystems). Afterwards slices were transferred to 34°C cutting solution for 45 minutes to recover and finally maintained at RT until used for recordings.
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bioRxiv - Genomics 2021Quote: ... Serial sections cut to a thickness of 5 μm were obtained using a microtome (Leica RM2125RT, Leica Microsystems Nussloch GmbH, Germany) and stained in Gill 2 hematoxylin (Thermo Scientific Kalamazoo ...
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bioRxiv - Microbiology 2021Quote: ... collecting ∼200 000 bright-field images at 5 ms resolution with a 100× oil objective on a Leica DMi8 inverted microscope (Leica, Germany) at a set temperature value of 30°C.
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bioRxiv - Microbiology 2021Quote: ... Images of the colony patterns were taken under a 5× objective using a Leica DM6000B fluorescence microscope (Leica Corporation, Wetzlar, Germany) equipped with a LED fluorescence illuminator (Leica Corporation) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were placed in an incubator chamber at 37 °C with 5% CO2 supplementation mounted on a Leica TCS SP8X microscope system (Leica Microsystems). Laser micro–irradiation was performed using a protocol adapted from (6) ...
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bioRxiv - Cell Biology 2021Quote: ... and xz images were acquired in the presence of the membrane dye CellMask (5 mg/ml, Life Technology) using Leica TCS SP5X confocal microscope (Leica Microsystems). The MFI of Fluo-4 in the cytoplasmic region in individual cells was measured using the NIH ImageJ software ...
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bioRxiv - Bioengineering 2022Quote: The liver with repopulated bile ducts of GFP-expressing cells was cultured in the circulation culture system for 5 days and imaged using confocal microscopy (Leica Microsystems). A reconstructed 3D image was prepared from Z-stack images using ImageJ (National Institutes of Health).
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bioRxiv - Microbiology 2020Quote: ... were visualized in the phase-contrast mode with a Leica DMi8 microscope with a 20X objective and an attached environment control chamber (37°C with 5% CO2) (Leica Microsystems). For each Transwell ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of DAPI-stained samples were used for fluorescence microscopy observation under the Leica TCS SP8 confocal microscope (Leica, Germany).
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended in 80 µl of the M solution and 5 µl were used for fluorescence microscopy observation under the Leica TCS SP8 confocal microscope (Leica, Germany). The data was analyzed using the Leica Application Suite X imaging and analysis software (Leica ...
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bioRxiv - Microbiology 2022Quote: ... The target probe was validated on reproductive tracts from mice experimentally inoculated with Chlamydia muridarum strain Nigg.5 Slides were stained on an automated stainer (Leica Bond RX, Leica Biosystems) with RNAscope 2.5 LS Assay Reagent Kit-Red (322150 ...
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bioRxiv - Neuroscience 2020Quote: ... and 25 mM dextrose) with bubbling 95% O2 and 5% CO2 using a vibratome (VT1000 S, Leica Microsystems, Buffalo Grove, IL). Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... For immunohistochemistry 5 μm thick serial sections were cut at −23°C using a Leica CM3050 cryostat (Leica Microsystems, Wetzlar, Germany) and collected in Superfrost® Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: All experiments were imaged using a Leica SP-5 confocal microscope using a Plan-Apochromatic 63x 1.40 NA oil-immersion objective (Leica microsystems, 506210). Fluorophores were excited using a 100 mW Ar laser (458 ...
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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bioRxiv - Cell Biology 2022Quote: ... lensectomy and sclerectomy were performed and retinas embedded in 5% agar and 150 µm sections made using a fully automated VT1200S vibrating blade microtome (Leica Biosystems). Sections were cultured in retinal media (DMEM extra (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... at 5 µm in thickness with a tungsten carbide blade (SL-T30, SECTION LAB Co. Ltd) using a cryostat system (Leica CM1850). The undecalcified sections attached to the adhesive film were immerged in 100% ethanol for 30 sec to remove the compound and air-dried ...