Labshake search
Citations for Leica :
801 - 850 of 917 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 at 10 °C and maintained at 90% humidity and then plunge frozen into liquid ethane (−180 °C) using a Leica GP plunger (Leica Microsystems, Austria).
-
bioRxiv - Physiology 2022Quote: ... Samples were embedded in the Optimal Cutting Temperature compound and sectioned to 20–30 µm at 50 °C using a cryostat (Leica CM 3050S) and stained with haematoxylin & eosin using Sakuras Tissue-Tek DRS 2000 at the Department of Anatomy ...
-
bioRxiv - Pathology 2022Quote: ... Slides were dried in a 60 °C oven for 30 min and mounted with a permanent mounting medium (Micromount®, Leica Biosystems). Lung sections from a SARS-CoV-2-infected hamster were used as positive assay controls.
-
bioRxiv - Molecular Biology 2023Quote: ... Vitreous sectioning was performed on infected cells tubes containing the vitrified sample pre-cooled at −140°C on an EM UC6/ FC7 cryo-ultramicrotome (Leica Microsystems, Vienna). The sample was trimmed to a pyramidal shaped block of 140 μm base and approximately 50 μm height using a 45° cryo trimming diamond knife (CT441 ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen in methylbutane solution (−50 to −60 °C) and sectioned into 40 μm serial sections with a freezing microtome (Leica SM 2010R). Serial sections spaced 240 μm apart representing the entire forebrain were collected in a 6-well tray using a freezing microtome (Stoyka et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... 30 μm) embedded in TissueTek (Sakura Finetek, California, USA) and sectioned at -20°C on a Leica CM1950 cryostat (Leica, Wetzlar, Germany).
-
bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Photographs of the thorax and wings were taken with a stereomicroscope (Leica M125 C; Camera: Leica MC 190 HD; Software: LAS V4.12). For the photographs the forewings and hindwings were mounted on a glass slide using a coverslip ...
-
bioRxiv - Cancer Biology 2024Quote: ... The slides were heated at 60°C for 1 h in an oven before H&E staining in the autostainer (Leica Autostainer XL). IHC was performed using a detection kit (Vector Laboratories ...
-
bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at –180 °C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
-
bioRxiv - Neuroscience 2023Quote: ... The brains were frozen using dry ice to preserve at - 80°C and sectioned at a thickness of 40 μm using a cryostat (CM3050S, Leica Biosystems, Germany).
-
bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at -180°C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
-
bioRxiv - Microbiology 2022Quote: ... Grids were blotted for 5 s and plunge-frozen in liquid ethane cooled by liquid nitrogen to approximately -195 °C using an EM GP Plunge Freezer (Leica, Wetzlar, Germany).
-
bioRxiv - Physiology 2023Quote: ... and imaged at 25°C in a glass-bottomed dish using an inverted confocal microscope (Leica SP8 DMi8, Leica Camera, Wetzlar, Germany) at 63x magnification (HC PL Apochromat C52 Oil objective ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
-
bioRxiv - Cell Biology 2023Quote: ... Seventy nm-thick frozen-hydrated sections were cut at −150°C in a Leica UC7/FC7 cryo-ultramicrotome (Leica Microsystems, Vienna, Austria). The EM grid was positioned underneath the ribbon using a Leica micromanipulator (Studer et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... then cryoprotected with 20% sucrose in PBS for 24 hours at 4°C and cut in 5-µm-consecutive thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picro Sirius Red Stain Kit (Abcam ...
-
bioRxiv - Microbiology 2023Quote: ... The dehydrated hNEC transwell membrane was then separated using a surgical blade and incubated in 65 °C paraffin (Leica EM-400 3801320) for 30 mins ...
-
bioRxiv - Bioengineering 2023Quote: ... The postfixed brains were frozen in Tissue Tek OCT at −80 °C and sliced coronally into 20 μm sections with a cryostat machine (CM1900, Leica Microsystems, USA). The sections were washed with 0.1 m PBS three times for 15 min each and blocked for 1 hour with a solution of 10% normal goat serum (Vector Laboratories ...
-
bioRxiv - Biophysics 2023Quote: DNA oligoPAINT FISH imaging was performed at 24 °C in a Leica TCS SP8 confocal microscope equipped with 100× 1.44 NA oil immersion objective (Leica, HC PLAN APO), two SPCM AQR (Perkin Elmer ...
-
bioRxiv - Immunology 2023Quote: ... Slides were analyzed and images were taken using a Leica DM4 B light microscope with a DFC450 C Microscope Digital Camera at 20X and the software Leica Application Suite V4.13 (Leica Microsystems, Wetzlar, Germany).
-
bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... Slides were baked at 60 °C for 15 min and deparaffinized with the Leica Bond Dewax solution (Leica Biosystems, Deer Park, IL), followed by heat-based antigen retrieval using Bond Epitope Retrieval Solution 1 (Leica Biosystems ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Resin blocks were stored in −20°C until trimming into 10 µm sections with a RM 2255 microtome (Leica Biosystems, Nussloch, Germany). Sections were placed on adhesive glass slides (Menzel-Gläser Superfrost® Plus ...
-
bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... cryoprotected in PBS with 30% sucrose for 24 h and kept at −80 °C until use for cryostat sectioning (40 µm-thick; Leica, CM 3050S). Sections were kept in cryoprotectant solution (20% glycerol ...
-
bioRxiv - Immunology 2024Quote: ... and tonsil sections of 4 µm-thick were deparaffinized at 70°C in 100% Clearene solvent (Leica Biosystems, Buffalo Grove, IL, USA) solution and washed in ethanol (EtOH ...
-
bioRxiv - Immunology 2024Quote: ... Brains were placed in 4 °C s-ACSF and sectioned with a vibrotome at 350–400 μm thick (Leica VT1200S; Leica, Nusslock, Germany). Collected slices were then incubated at 32 °C for 1 h in i-ACSF (124 mM/L NaCl ...
-
bioRxiv - Biophysics 2024Quote: ... alveolospheres were cultured in 35 mm petri dish in a customized incubator (5% CO2, 37°C, 95% humidity) on a confocal microscope (Leica, TCL SP8). A 25 × /0.95 numerical aperture water objective and a 63×/1.20 numerical aperture water objective were both used for imaging ...
-
bioRxiv - Microbiology 2024Quote: ... sucrose in PBS overnight at 4°C and embedded in OCT compound (Agar Scientific) and cryosectioned (10 µm thick, Leica CM1850 Cryostat). Sections were dried overnight onto Thermo Scientific SuperFrost Plus Adhesion slides ...
-
bioRxiv - Developmental Biology 2024Quote: ... PFA-fixed and paraffin embedded tissue slides (5 µm) were incubated for 1h at 60°C with Bond Dewax solution (Leica Bond, AR9222) to deparaffinize tissue sections ...
-
bioRxiv - Microbiology 2024Quote: ... Slides were dried in a 60 °C oven for 30 min and mounted with a permanent mounting medium (Micromount®, Leica Biosystems). Lung sections from a pig experimentally infected with swine influenza virus A/swine/Texas/4199-2/1998 H3N2 were used as a positive assay control.
-
bioRxiv - Neuroscience 2024Quote: ... Brains were then stored at −80°C for later sectioning or sectioned immediately on a Leica Micrtotome (Leica SM2010 R Sliding Microtome). Sections were 100 µm and stored in anti-freeze solution (30% glycerol ...
-
bioRxiv - Cell Biology 2024Quote: ... 3 µm apart) were acquired every 15 mins on a 37 °C temperature controlled (Cube & Box, CH) Leica SP8x confocal microscope (Leica Microsystems, Germany) equipped with a dry Leica 40x/0.85NA HCX PL APO CS ...
-
bioRxiv - Genetics 2021Quote: FFPE tissue sections were cut using a microtome and baked at 62°C for 20 minutes followed by haematoxylin and eosin (H&E) staining using a Leica Autostainer XL (Leica Microsystems, Wetzlar, Germany). Slides were mounted with DPX (distyrene ...
-
bioRxiv - Immunology 2021Quote: ... The organs were then embedded into paraffin wax using a HistoCore Arcadia C and H paraffin embedding station (Leica Biosystems, Buffalo Grove, IL). Each organ was sliced into 4 µm sheets using a RM2235 manual microtome (Leica Biosystems ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
-
bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
-
bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
-
bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
-
bioRxiv - Microbiology 2021Quote: ... the excess of solution was manually blotted using a Whatman filter paper and then the grid was plunge-frozen into liquid ethane cooled down at - 174°C by liquid nitrogen in a Leica EM CPC cryo-plunger (Leica Microsystems, Wetzlar, Germany). Three grids were prepared per condition ...
-
bioRxiv - Microbiology 2020Quote: ... While keeping the orientation succession the slices were further processed into paraffin blocks using the Leica ASP6025 tissue processor and Leica EG1150H+C tissue embedding centre (both Leica Biosystems, Nussloch, Germany). Three serial adjacent 2.5 μm thick sections were then cut with the Leica RM 2255 Microtome (Leica Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were removed from freshly euthanized mice and placed in 10% formalin at 4 °C for 24 h before embedding in paraffin blocks and preparing 4-μm tissue sections using a microtome (Leica Microsystems, Wetzlar, Germany). We detected MAGL mRNA in situ using the RNAscope 2.5 Assay for Ventana Discovery Ultra system (ACD Bio ...
-
bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
-
bioRxiv - Pathology 2022Quote: ... Heat-induced epitope retrieval (HIER) was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
-
bioRxiv - Neuroscience 2022Quote: ... Cryoprotected spinal cords were embedded in optimal cutting temperature (OCT) compound and frozen at −80 °C until cryostat sectioning (Leica Lm 1850, Germany). Transverse lumbar spinal cord cryosections were collected in serial at 30-μm or 40-μm thickness ...
-
bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...