Labshake search
Citations for Leica :
701 - 750 of 917 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems). Images containing complexes were collected manually with a 120 kV TALOS transmission electron microscope (Thermo Fisher Scientific ...
-
Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: ... and vitrification using a Leica GP2 plunger equipped with a blotting detection sensor and incubation chamber maintaining an environment of 21°C and 90% humidity (Leica Microsystems). Grids were placed into the GP2 incubation chamber and excessive liquid was manually removed with a piece of filter paper by gently touching the side of the grid ...
-
bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
-
bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
-
bioRxiv - Neuroscience 2023Quote: ... containing the dorsal hippocampus were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... sagittal sections at 5° angle tilted to the midline with 300 μm thickness were obtained inside the same slicing solution at 4°C using Leica VT1200S vibrotome (Leica Biosystems). Slices were immediately transferred to a chamber filled with artificial cerebrospinal fluid (ACSF ...
-
bioRxiv - Immunology 2022Quote: ... the tissue slides were heat pre-treated using a ER1 (pH6) target retrieval buffer for 15 minutes at 97°C (AR9961, Leica Biosystems). The slides were then stained for multiplex immunofluorescence using the BOND RX Opal multiplex procedure (v7.0.3.216 RX ...
-
bioRxiv - Microbiology 2022Quote: ... Cryo-sections (70 nm thick) of the frozen material were obtained at −120°C using an Ultracut cryo-ultramicrotome (Leica Microsystems). The cryo-sections were collected on formvar-coated nickel grids ...
-
bioRxiv - Microbiology 2023Quote: ... samples were stored light-protected in the same buffer at 4°C until imaging with a Leica TCS SP5 II laser scanning confocal microscope (Leica Microsystems) using sequential scanning mode.
-
bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with half seed in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems). with vacuum infiltration ...
-
bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with dozens of seeds in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems) with vacuum infiltration ...
-
bioRxiv - Neuroscience 2023Quote: ... thin coronal midbrain cryosections (12 µm) were cut at -20°C and transferred to RNaseZAP-treated PPS FrameSlides (Leica Microsystems, # 11600294). Six sections per FrameSlide ...
-
bioRxiv - Developmental Biology 2023Quote: ... and embedded tissue was frozen rapidly and either stored at − 80 °C or immediately sectioned into 20-μm thick frozen sections using a cryostat (Leica CM3050S). Histological sections were placed in Superfrost® slides and placed at -20°C ...
-
bioRxiv - Pathology 2023Quote: Carriers containing high pressure frozen samples for cryoFIB-SEM liftout were transferred to a cryo-ultramicrotome (Leica EM FC7, −160°C) equipped with a Trim 45 T1865 diamond knife ...
-
bioRxiv - Neuroscience 2023Quote: ... The L4 segments were fixed overnight at 4°C and cut into 60 μm thick transverse sections with a vibrating blade microtome (Leica VT1200). The sections were incubated for 3 days in rabbit anti-GFP (see Table S2) ...
-
bioRxiv - Bioengineering 2023Quote: ... the tissue was placed in a cryostat chamber at -20°C and sliced into 15 µm thick sections (CM1850, Leica microsystems). The FBR to the polymer coatings was assessed by staining with haematoxylin and eosin (H&E ...
-
bioRxiv - Neuroscience 2022Quote: C57Bl/6J wild-type mice were anesthetized with isoflurane and brains were rapidly dissected and frozen on dry ice in OCT embedding medium and stored at -80°C until being sectioned on a cryostat (Leica CM3050s). Ten-micron sections were collected at -20°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Mouse brain was embedded and frozen in optimal cut temperature (OCT) (SAKURA, USA) compound at - 80°C and 10 μm cryosection slices were prepared by using a Leica cryostat (Leica, Germany). The slices were warmed up to room temperature and fixed with 4% PFA for 5 min before used ...
-
bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
-
bioRxiv - Neuroscience 2024Quote: ... brains were immediately frozen and stored at −80°C until sectioning at 12 μm on a cryostat (Leica Biosystems, Wetzlar, DE). PVH and ARC tissue was collected from brain sections using laser capture microdissection with a PALM Robot Microbean (Carl-Zeiss ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...
-
bioRxiv - Neuroscience 2024Quote: ... Cultures were inspected with the microscope Leica DM IL Led Fluo and pictures acquired with camera Leica DFC450 C (Leica Microsystem).
-
bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...
-
bioRxiv - Microbiology 2023Quote: ... as described above, the cells were incubated (37°C, 5% CO2) inside the Mica Microhub system using the internal incubator (Leica microsystems). Videos were analysed using the complementary software (Leica microsystems).
-
bioRxiv - Molecular Biology 2023Quote: Adult flies containing P[GMR-Gal4] and four copies of P[UAS-arm*] or its variants were frozen at −20°C overnight and photographed with a Leica Stereo Dissecting Scope (Leica DMI6000B) attached to a digital camera ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue blocks were warmed to −20°C for at least 1h prior to sectioning and sectioned at 18 µm using a cryostat (Leica CM3050S) at −20°C ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were frozen at -20⍰C and cryosections with 30 μm thickness were prepared Leica CM1950 cryomicrotome (Leica BioSystems, Diegem, Belgium). Slides were then thawed on Superfrost glass slides (Epredia ...
-
bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval was carried out with TRIS buffer at pH 9 for 20 mins at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems). HA and Myc tag wer detected using anti-HA (see above ...
-
bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...
-
bioRxiv - Systems Biology 2023Quote: Frozen serial 8 μm sections of mice heart tissues were cut at −20°C with a Microtome (CM1950; Leica, Nussloch, Germany) and mounted on ITO-coated glass slides for MALDI-MSI and on coated glass slides for HE staining ...
-
bioRxiv - Neuroscience 2023Quote: ... snap-frozen in powdered dry ice and stored at -80 °C until sectioned at 40 µm in the coronal plane with a Leica SM 2000R freezing microtome (Leica Microsystems). The sections were stored at -20 °C in 24-well tissue culture plates filled with antifreeze solution (30% ethylene glycol ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000; Ted Pella, or VT1000P; Leica). Sections were collected in cryoprotectant (25% glycerol ...
-
bioRxiv - Immunology 2024Quote: ... tissues were subsequently embedded in OCT and stored at –80 °C before sectioning (6 μm) on a Leica CM1850 Cryostat (Leica Biosystems). Sections were incubated for 1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... timelapse phase contrast images were acquired for 2 h at 37 °C with a 10X or 20X fluotar objective on a Leica DMIRE2 inverted microscope (Leica, Germany) equipped with a MS-2000 XYZ automated stage (ASI ...
-
bioRxiv - Biochemistry 2022Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge-frozen in liquid ethane at −181 °C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using a Tecnai G2 (FEI ...
-
bioRxiv - Biophysics 2022Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge-frozen in liquid ethane at –181 °C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using a Tecnai G2 (FEI ...
-
bioRxiv - Biochemistry 2022Quote: ... polymerized at 70°C for 24 hours and then cut to 70 nm ultra-thin sections with an Ultracut S ultramicrotome (Leica, Germany). After cutting ...
-
bioRxiv - Neuroscience 2022Quote: ... for 5 s followed by high-pressure freezing after 25 s at -180°C under 2100 bar pressure in an HPM100 machine (Leica Microsystems). After freezing ...
-
bioRxiv - Molecular Biology 2022Quote: ... the grid was back-blotted and immediately plunge frozen in liquid ethane at −182°C (Leica Automatic Plunge Freezer EM GP2).
-
bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
-
bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
-
bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were placed in a 37°C chamber incubator supplemented with 5% CO2 mounted on Leica TCS SP8X microscope system (Leica Microsystems).
-
bioRxiv - Cell Biology 2023Quote: The frozen blocks were removed from the-80°C freezer and allowed to sit in the cryostat (Leica CM 1950, #047742456) at-20°C for 1 hour to equilibrate ...
-
bioRxiv - Neuroscience 2023Quote: The right hemisphere of each brain was sliced into 300μm coronal sections at −10°C using a cryostat (CM3050 S; Leica, Nuβloch, Germany). Punches of 0.75mm and 1.25mm were used to extract tissue from the frontal cortex ...
-
bioRxiv - Cell Biology 2024Quote: ... The frozen specimens were freeze-substituted in anhydrous acetone containing 2% osmium tetroxide (OsO4) at -80°C for 72 hours in an AFS2 machine (Leica Microsystems). After being slowly warmed to RT over 48 hours ...
-
bioRxiv - Biochemistry 2024Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...