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Citations for Leica :
651 - 700 of 917 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S ...
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired every 15 min at 30°C for 17 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL 614 APO 63x/1.4 Oil ph3 objective Leica) ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections of the IC (200 μm) were cut in 34°C ACSF with a vibrating microtome (VT1200S, Leica Biosystems) and incubated at 34°C for 30 min in ACSF bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in PFA overnight at 4 °C and dehydrated in 30% sucrose (in PBS) for at least 18 hours before vibratome sectioning (VT1000s, Leica). Brains were cut into 50 µm coronal section using cryostat (CM 1950 ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope antigen retrieval was performed at 100°C for 20 min in Bond Epitope Retrieval Solution 1 (Cat#AR9961, Leica) for GRPR or Bond Epitope Retrieval Solution 2 (1/100 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was snap frozen in OCT and stored at −70°C until sectioning in to 12 µm slices on a cryostat (Leica). Thaw-mounted sections were stored at −70°C ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Genomics 2024Quote: ... on the Leica Bond RXm system by deparaffinization and heat-induced epitope retrieval (HIER) at 100 °C for 15 min using ER2 epitope retrieval buffer (Leica Biosystems product ...
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bioRxiv - Plant Biology 2024Quote: ... the root tips were substituted with 2% osmium tetroxide in anhydrous acetone and maintained at -80°C for 24 hours using an AFS2 temperature-controlling system (Leica). Subsequently ...
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bioRxiv - Physiology 2024Quote: ... 5-µm-thick transverse sections were cut at the mid-diaphysis on a cryostat set to -30 to -35°C (Leica) using a modified tape transfer protocol based on (27) ...
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bioRxiv - Immunology 2024Quote: ... Brains were then frozen in OCT and stored at −80°C until sectioning into 40 μm sections using a cryostat (Leica). 40 μm sections were then stored in 1X PBS containing 0.05% NaN3 at 4°C.
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bioRxiv - Neuroscience 2024Quote: ... Frozen sections were immersed in 0.5% uranyl acetate in methanol at –90°C in the AFS freeze-substitution unit (Leica Microsystems), infiltrated at –45°C with Lowicryl HM-20 resin (Chemische Werke Lowi ...
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bioRxiv - Bioengineering 2024Quote: ... at 4°C and embedded in OCT for generation of 15 µm coronal sections using a CM3050 S cryostat (Leica).
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bioRxiv - Developmental Biology 2024Quote: ... Included tendon constructs were then frozen at -80°C in isopentane for 1 minute and 12µm cryosections were generated with a CM3050 S Cryostat (Leica Biosystems).
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bioRxiv - Immunology 2024Quote: ... Slides were baked in an oven at 70°C for 60 minutes and then loaded onto an automated stainer (Leica Bond III for CD4 staining and Dako Omnis (Dako / Agilent ...
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bioRxiv - Physiology 2024Quote: TA muscles were cryopreserved in optimal cutting temperature compound (Sakura Finetek) using liquid nitrogen-cooled isopentane and cryosectioned at 8µm (−18°C, Leica CM1950). Mounted sections were stained with haematoxylin and eosin (H&E ...
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bioRxiv - Plant Biology 2024Quote: ... the root tips were substituted with 2% osmium tetroxide in anhydrous acetone and maintained at −80 °C for 24 hours using an AFS2 temperature-controlling system (Leica). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... were added before back-side blotting and plunge freezing into liquid ethane at −185 °C using a GP2 (Leica Microsystems). The plunge-frozen grids were clipped into FIB-AutoGrids (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... samples were freeze-substituted at -90°C in anhydrous acetone containing 2% osmium tetroxide and 0.1% uranyl acetate at -90°C for 72 hours and warmed to room temperature with a gradient of +5°C per hour (EM AFS-2, Leica). Sections of the samples were analysed in a Tecnai Spirit T12 Electron microscope equipped with an Eagle OCD camera (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... Frozen pellets were immersed in 0.5% uranyl acetate in methanol at −90°C in an AFS freeze-substitution unit (Leica Microsystems), infiltrated at −45°C with Lowicryl HM-20 resin (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica, Wetzlar, Germany). After gradient infiltration with increasing concentration of HM20 ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems). The SARS-CoV-2 probe (catalog number 848568 ...
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bioRxiv - Cell Biology 2022Quote: ... Live-cell imaging was performed at 37°C with a Leica TCS SP8 confocal microscope with a 100×/1.40 Oil HC PL APO CS2 objective (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...
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bioRxiv - Immunology 2021Quote: ... Samples were blotted for 3-4 s at 80% humidity and 20°C and subsequently plunge-frozen in liquid ethane using an EM GP blotter (Leica Microsystems). Grids containing NLRP3PYD filaments were imaged using a Krios Titan TEM (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Bioengineering 2020Quote: ... The diffusion experiments were performed in the environmentally controlled chamber (set at 37 °C, 5% CO2) of a fluorescent microscope (Leica, CTR6500). The dextran solution was perfused inside the microvessel channel and images were acquired every 30 sec over 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
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bioRxiv - Neuroscience 2021Quote: ... Confocal and STED images were acquired at 23°C with a modified TCS SP5 STED-CW gated (Leica Microsystems, Mannheim, Germany) operated with Leica’s microscope imaging software ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Biophysics 2020Quote: ... the grids were lifted and held by tweezers inside the plunge freezer chamber at 90% relative humidity and 22 °C (Leica EM-GP plunger, Leica Microsystems). 1 μL drop of X5 concentrated gold nanoparticle (150 nm ...
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bioRxiv - Neuroscience 2020Quote: ... The fly head-embedded gelatin was subsequently cut with 12 μm thickness using a cryo-microtome at −20°C (Leica CM1520). Sections were thaw-mounted on indium tin oxide coated glass and freeze-dried overnight in the ToF-SIMS instrument for further analysis.
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bioRxiv - Plant Biology 2021Quote: ... The abaxial side of cotyledons were imaged using a 63x/1.2 water immersion objective lens (Leica C-APOCHROMAT 63×/1.2 water). GFP was excited at 488 nm and fluorescence emission collected between 500-540nm ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Cell Biology 2021Quote: ... Samples were embedded at 60°C for 48 h and cut into 70 nm sections with a microtome (EM UC7, Leica, Germany). After electron staining with uranyl acetate and lead citrate ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were placed in an incubator chamber at 37 °C with 5% CO2 supplementation mounted on a Leica TCS SP8X microscope system (Leica Microsystems). Laser micro–irradiation was performed using a protocol adapted from (6) ...
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bioRxiv - Cell Biology 2022Quote: SMLM images were acquired at room temperature (24 °C) on a customized microscope (Mund et al., 2018) with a 160x NA 1.43 oil-immersion objective (Leica, Wetzlar, Germany). Illumination was done using a a LightHub laser combiner (Omicron-Laserage Laserprodukte ...
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bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then frozen in −20°C isopentane and cut into 20 μm-thick coronal sections on a cryostat (Leica CM1900), collected on silane-coated slides and stored at −20°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: ... were visualized in the phase-contrast mode with a Leica DMi8 microscope with a 20X objective and an attached environment control chamber (37°C with 5% CO2) (Leica Microsystems). For each Transwell ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were cultured for 4 hours at 37 °C in the climate chamber of a Leica SP8 Confocal system (Leica Microsystems) microscope ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Systems Biology 2022Quote: FLIM of EGFR-mCitrine/PTB-mCherry or PTP-mCitrine/DyTo was performed at 37°C on the Leica SP8 laser-scanning microscope equipped with a fast lifetime contrast module (FALCON, Leica Microsystems) using the 63×/1.4NA oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Genomics 2022Quote: ... tissue slides were baked overnight at 60°C to ensure tissue adherence to the positively charged glass slides (Leica Bond Plus Microscope slides, Leica Biosystems). Then the tissue was deparaffinized and digested with Protease Plus (ACD Bio ...
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bioRxiv - Developmental Biology 2021Quote: ... For immunohistochemistry 5 μm thick serial sections were cut at −23°C using a Leica CM3050 cryostat (Leica Microsystems, Wetzlar, Germany) and collected in Superfrost® Plus slides (Thermo Fisher Scientific ...