Labshake search
Citations for Leica :
801 - 850 of 1308 citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The number of protoplasts was counted with the C-Chip Neubauer improved hemocytometer (NanoEnTek, Seoul) under a light microscope (Leica VT1000, Wetzlar). The viability of protoplasts was determined using trypan blue solution (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... embedded in Tissue Tek® and after freezing at −80°C cut in 10 μm cross-sections using a Cryostat Microm HM560 (Leica Biosystems).
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bioRxiv - Cell Biology 2022Quote: ... The frozen sample were transferred to cryovials in liquid nitrogen vapor and transferred to a pre-cooled (−90°C) freeze substitution unit (Leica EM AFS). Freeze substitution was performed using a mixture of 0.12% aqueous Uranyl acetate in anhydrous acetone using the following program ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 4% PFA for 24 hours at 4°C and sectioned (20-60 μm) using a vibratome (Leica VT 1000s). If necessary ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S, Leica, Wetzlar, Germany) in 50 µm slices ...
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bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
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bioRxiv - Bioengineering 2022Quote: ... frozen human brains were sliced at a temperature of −30 °C into 60 μm-deep coronal sections using a cryostat microtome (Leica Microsystems, Germany). Finally ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were postfixed in 4% paraformaldehyde overnight on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S), with sections collected into six tubes (containing cryoprotectant solution as described[32] ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2021Quote: ... the chambered slide was imaged using Leica SP5 inverted confocal microscope with environmental chamber to maintain the sample humidified at 37°C (Leica Microsystems, USA). Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems ...
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bioRxiv - Cell Biology 2020Quote: ... The grids were manually blotted using Whatman filter paper and plunge-frozen into liquid ethane at −174°C using a Leica EM-CPC equipment (Leica, Wetzlar, Germany). After freezing ...
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bioRxiv - Immunology 2020Quote: ... at which time tissues were removed from the BSL-3 laboratory and stored in 1X PBS at 4°C until processed in a Leica PELORIS automated vacuum infiltration processor (Leica, Wetzlar, Germany), followed by paraffin embedding with a Histocore Arcadia paraffin embedding machine (Leica) ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 at 10 °C and maintained at 90% humidity and then plunge frozen into liquid ethane (−180 °C) using a Leica GP plunger (Leica Microsystems, Austria).
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bioRxiv - Physiology 2022Quote: ... Samples were embedded in the Optimal Cutting Temperature compound and sectioned to 20–30 µm at 50 °C using a cryostat (Leica CM 3050S) and stained with haematoxylin & eosin using Sakuras Tissue-Tek DRS 2000 at the Department of Anatomy ...
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bioRxiv - Pathology 2022Quote: ... Slides were dried in a 60 °C oven for 30 min and mounted with a permanent mounting medium (Micromount®, Leica Biosystems). Lung sections from a SARS-CoV-2-infected hamster were used as positive assay controls.
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bioRxiv - Molecular Biology 2023Quote: ... Vitreous sectioning was performed on infected cells tubes containing the vitrified sample pre-cooled at −140°C on an EM UC6/ FC7 cryo-ultramicrotome (Leica Microsystems, Vienna). The sample was trimmed to a pyramidal shaped block of 140 μm base and approximately 50 μm height using a 45° cryo trimming diamond knife (CT441 ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen in methylbutane solution (−50 to −60 °C) and sectioned into 40 μm serial sections with a freezing microtome (Leica SM 2010R). Serial sections spaced 240 μm apart representing the entire forebrain were collected in a 6-well tray using a freezing microtome (Stoyka et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The brains were frozen using dry ice to preserve at - 80°C and sectioned at a thickness of 40 μm using a cryostat (CM3050S, Leica Biosystems, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at -180°C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Microbiology 2022Quote: ... Grids were blotted for 5 s and plunge-frozen in liquid ethane cooled by liquid nitrogen to approximately -195 °C using an EM GP Plunge Freezer (Leica, Wetzlar, Germany).
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bioRxiv - Physiology 2023Quote: ... and imaged at 25°C in a glass-bottomed dish using an inverted confocal microscope (Leica SP8 DMi8, Leica Camera, Wetzlar, Germany) at 63x magnification (HC PL Apochromat C52 Oil objective ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Cell Biology 2023Quote: ... Seventy nm-thick frozen-hydrated sections were cut at −150°C in a Leica UC7/FC7 cryo-ultramicrotome (Leica Microsystems, Vienna, Austria). The EM grid was positioned underneath the ribbon using a Leica micromanipulator (Studer et al. ...
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bioRxiv - Microbiology 2023Quote: ... The dehydrated hNEC transwell membrane was then separated using a surgical blade and incubated in 65 °C paraffin (Leica EM-400 3801320) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... The postfixed brains were frozen in Tissue Tek OCT at −80 °C and sliced coronally into 20 μm sections with a cryostat machine (CM1900, Leica Microsystems, USA). The sections were washed with 0.1 m PBS three times for 15 min each and blocked for 1 hour with a solution of 10% normal goat serum (Vector Laboratories ...
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bioRxiv - Biophysics 2023Quote: DNA oligoPAINT FISH imaging was performed at 24 °C in a Leica TCS SP8 confocal microscope equipped with 100× 1.44 NA oil immersion objective (Leica, HC PLAN APO), two SPCM AQR (Perkin Elmer ...
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bioRxiv - Immunology 2023Quote: ... Slides were analyzed and images were taken using a Leica DM4 B light microscope with a DFC450 C Microscope Digital Camera at 20X and the software Leica Application Suite V4.13 (Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were baked at 60 °C for 15 min and deparaffinized with the Leica Bond Dewax solution (Leica Biosystems, Deer Park, IL), followed by heat-based antigen retrieval using Bond Epitope Retrieval Solution 1 (Leica Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Resin blocks were stored in −20°C until trimming into 10 µm sections with a RM 2255 microtome (Leica Biosystems, Nussloch, Germany). Sections were placed on adhesive glass slides (Menzel-Gläser Superfrost® Plus ...
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bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were embedded in OCT and frozen on dry ice and stored at -80°C until sectioning on a cryostat (Leica CM3050-S). Brain sections were mounted at 10 μm thickness on Superfrost Plus slides (1255015 ...
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bioRxiv - Microbiology 2023Quote: ... Slides were dried in a 60 °C oven for 30 min and mounted with a permanent mounting medium (Micromount®, Leica Biosystems). Lung sections from a pig experimentally infected with swine influenza virus A/swine/Texas/4199-2/1998 H3N2 were used as a positive assay control.
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at –180 °C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Neuroscience 2023Quote: ... 30 μm) embedded in TissueTek (Sakura Finetek, California, USA) and sectioned at -20°C on a Leica CM1950 cryostat (Leica, Wetzlar, Germany).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Photographs of the thorax and wings were taken with a stereomicroscope (Leica M125 C; Camera: Leica MC 190 HD; Software: LAS V4.12). For the photographs the forewings and hindwings were mounted on a glass slide using a coverslip ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were heated at 60°C for 1 h in an oven before H&E staining in the autostainer (Leica Autostainer XL). IHC was performed using a detection kit (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... The frozen samples were stored at -20°C in preparation for tissue sectioning using a cryostat (Leica CM3050 S, 10 μm thickness).
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bioRxiv - Cell Biology 2024Quote: ... Microscopy images were collected at 37 °C with a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DM I8 inverted epifluorescence microscope (Leica Microsystems, Germany), with a 63× 1.4 NA oil immersion lens (506350 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A series of time laps pictures was taken (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD) every 10s for 24h ...
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bioRxiv - Cancer Biology 2022Quote: ... The specimen frozen blocks were serially sectioned longitudinally at respectively 5 μm thickness using Cryostat microtome (Leica CM1950). Sections were used for H&E and immunofluorescence staining ...
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bioRxiv - Neuroscience 2019Quote: ... Regions of interest were delineated according to 5× magnification using a Leica DM4000B microscope (Leica, Concord, ON, Canada). Sections spanning plates 14–18 of the Paxinos & Franklin mouse atlas28 were analyzed ...