Labshake search
Citations for Leica :
751 - 800 of 1401 citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and cross-sectioned to a thickness of 5 µm using a Leica RM2025 microtome (Leica Microsystems, Germany). The obtained histological sections were initially subjected to topographic staining using the hematoxylin-eosin (H/E ...
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bioRxiv - Immunology 2023Quote: ... Counterstaining was performed with hematoxylin for 5 min and slides were finally mounted with Micromount (Leica Biosystems).
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bioRxiv - Cancer Biology 2024Quote: ... Confocal images were collected using a TCS-SP 5 spectrophotometric multiphoton laser scanning confocal microscope (Leica Microsystems) collecting 0.2 - 1 µm optical sections.
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bioRxiv - Cell Biology 2020Quote: ... prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica, Wetzlar, Germany). After gradient infiltration with increasing concentration of HM20 ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems). The SARS-CoV-2 probe (catalog number 848568 ...
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bioRxiv - Cell Biology 2022Quote: ... Live-cell imaging was performed at 37°C with a Leica TCS SP8 confocal microscope with a 100×/1.40 Oil HC PL APO CS2 objective (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Cell Biology 2019Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge frozen in liquid ethane at −181°C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using three different microscopes ...
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bioRxiv - Neuroscience 2019Quote: ... brains were flash frozen using crushed dry ice and coronal sections (40 μm) containing the BNST were collected using a cryostat set to −20° C (Leica Microsystems). Sections were wet-mounted onto gelatin-subbed glass microscope slides ...
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bioRxiv - Biochemistry 2019Quote: ... The grids were blotted for 2 s at 25 °C under 95% humidity before being plunged into liquid ethane using a Leica EM GP2 (Leica Microsystems). The grids were stored in liquid nitrogen before data acquisition.
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bioRxiv - Microbiology 2019Quote: ... After 24h incubation at 37°C images of the halo zones were taken using a Leica S8APO stereomicroscope (Leica, Wetzlar, Germany). The area of halo zones was determined by image analysis in Inkscape (version 0.91 ...
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bioRxiv - Cell Biology 2019Quote: ... back-blotted on the sample-free side with filter paper for 2 sec (22 °C, 90% humidity) and then plunged into liquid ethane using the EM Grid Plunger (Leica Microsystems). Electron micrographs for the cryo-ET study were collected with a 200 kV TF20 transmission electron microscope (FEI ...
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...
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bioRxiv - Immunology 2021Quote: ... Samples were blotted for 3-4 s at 80% humidity and 20°C and subsequently plunge-frozen in liquid ethane using an EM GP blotter (Leica Microsystems). Grids containing NLRP3PYD filaments were imaged using a Krios Titan TEM (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
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bioRxiv - Neuroscience 2021Quote: ... Confocal and STED images were acquired at 23°C with a modified TCS SP5 STED-CW gated (Leica Microsystems, Mannheim, Germany) operated with Leica’s microscope imaging software ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Biophysics 2020Quote: ... the grids were lifted and held by tweezers inside the plunge freezer chamber at 90% relative humidity and 22 °C (Leica EM-GP plunger, Leica Microsystems). 1 μL drop of X5 concentrated gold nanoparticle (150 nm ...
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bioRxiv - Neuroscience 2020Quote: ... The fly head-embedded gelatin was subsequently cut with 12 μm thickness using a cryo-microtome at −20°C (Leica CM1520). Sections were thaw-mounted on indium tin oxide coated glass and freeze-dried overnight in the ToF-SIMS instrument for further analysis.
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bioRxiv - Plant Biology 2021Quote: ... The abaxial side of cotyledons were imaged using a 63x/1.2 water immersion objective lens (Leica C-APOCHROMAT 63×/1.2 water). GFP was excited at 488 nm and fluorescence emission collected between 500-540nm ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Cell Biology 2021Quote: ... Samples were embedded at 60°C for 48 h and cut into 70 nm sections with a microtome (EM UC7, Leica, Germany). After electron staining with uranyl acetate and lead citrate ...
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bioRxiv - Cell Biology 2022Quote: SMLM images were acquired at room temperature (24 °C) on a customized microscope (Mund et al., 2018) with a 160x NA 1.43 oil-immersion objective (Leica, Wetzlar, Germany). Illumination was done using a a LightHub laser combiner (Omicron-Laserage Laserprodukte ...
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bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then frozen in −20°C isopentane and cut into 20 μm-thick coronal sections on a cryostat (Leica CM1900), collected on silane-coated slides and stored at −20°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Bioengineering 2020Quote: ... and cells were cultured for 4 hours at 37 °C in the climate chamber of a Leica SP8 Confocal system (Leica Microsystems) microscope ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Systems Biology 2022Quote: FLIM of EGFR-mCitrine/PTB-mCherry or PTP-mCitrine/DyTo was performed at 37°C on the Leica SP8 laser-scanning microscope equipped with a fast lifetime contrast module (FALCON, Leica Microsystems) using the 63×/1.4NA oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Genomics 2022Quote: ... tissue slides were baked overnight at 60°C to ensure tissue adherence to the positively charged glass slides (Leica Bond Plus Microscope slides, Leica Biosystems). Then the tissue was deparaffinized and digested with Protease Plus (ACD Bio ...
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bioRxiv - Plant Biology 2019Quote: ... T1 plants were grown under long day conditions at 22 °C and phenotyped using the z-stack function of a Leica M205 FA microscope (Leica, www.leica.de).
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bioRxiv - Biochemistry 2020Quote: ... Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems). Images containing complexes were collected manually with a 120 kV TALOS transmission electron microscope (Thermo Fisher Scientific ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: ... and vitrification using a Leica GP2 plunger equipped with a blotting detection sensor and incubation chamber maintaining an environment of 21°C and 90% humidity (Leica Microsystems). Grids were placed into the GP2 incubation chamber and excessive liquid was manually removed with a piece of filter paper by gently touching the side of the grid ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2022Quote: ... timelapse phase contrast images were acquired for 2 h at 37 °C with a 10X or 20X fluotar objective on a Leica DMIRE2 inverted microscope (Leica, Germany) equipped with a MS-2000 XYZ automated stage (ASI ...
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bioRxiv - Biochemistry 2022Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge-frozen in liquid ethane at −181 °C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using a Tecnai G2 (FEI ...
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bioRxiv - Biophysics 2022Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge-frozen in liquid ethane at –181 °C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using a Tecnai G2 (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... polymerized at 70°C for 24 hours and then cut to 70 nm ultra-thin sections with an Ultracut S ultramicrotome (Leica, Germany). After cutting ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 s followed by high-pressure freezing after 25 s at -180°C under 2100 bar pressure in an HPM100 machine (Leica Microsystems). After freezing ...
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bioRxiv - Molecular Biology 2022Quote: ... the grid was back-blotted and immediately plunge frozen in liquid ethane at −182°C (Leica Automatic Plunge Freezer EM GP2).
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bioRxiv - Neuroscience 2022Quote: C57Bl/6J wild-type mice were anesthetized with isoflurane and brains were rapidly dissected and frozen on dry ice in OCT embedding medium and stored at -80°C until being sectioned on a cryostat (Leica CM3050s). Ten-micron sections were collected at -20°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse brain was embedded and frozen in optimal cut temperature (OCT) (SAKURA, USA) compound at - 80°C and 10 μm cryosection slices were prepared by using a Leica cryostat (Leica, Germany). The slices were warmed up to room temperature and fixed with 4% PFA for 5 min before used ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Neuroscience 2024Quote: ... brains were immediately frozen and stored at −80°C until sectioning at 12 μm on a cryostat (Leica Biosystems, Wetzlar, DE). PVH and ARC tissue was collected from brain sections using laser capture microdissection with a PALM Robot Microbean (Carl-Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were inspected with the microscope Leica DM IL Led Fluo and pictures acquired with camera Leica DFC450 C (Leica Microsystem).
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bioRxiv - Cell Biology 2024Quote: ... Microphotographs (N>=6 fish per genotype) were acquired in a Leica DM4000 B microscope coupled to a Leica DFC425 C camera (Leica Microsystems).