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Citations for Leica :
951 - 1000 of 1401 citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Microbiology 2021Quote: ... the excess of solution was manually blotted using a Whatman filter paper and then the grid was plunge-frozen into liquid ethane cooled down at - 174°C by liquid nitrogen in a Leica EM CPC cryo-plunger (Leica Microsystems, Wetzlar, Germany). Three grids were prepared per condition ...
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bioRxiv - Microbiology 2020Quote: ... While keeping the orientation succession the slices were further processed into paraffin blocks using the Leica ASP6025 tissue processor and Leica EG1150H+C tissue embedding centre (both Leica Biosystems, Nussloch, Germany). Three serial adjacent 2.5 μm thick sections were then cut with the Leica RM 2255 Microtome (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were removed from freshly euthanized mice and placed in 10% formalin at 4 °C for 24 h before embedding in paraffin blocks and preparing 4-μm tissue sections using a microtome (Leica Microsystems, Wetzlar, Germany). We detected MAGL mRNA in situ using the RNAscope 2.5 Assay for Ventana Discovery Ultra system (ACD Bio ...
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bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Pathology 2022Quote: ... Heat-induced epitope retrieval (HIER) was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Neuroscience 2022Quote: ... Cryoprotected spinal cords were embedded in optimal cutting temperature (OCT) compound and frozen at −80 °C until cryostat sectioning (Leica Lm 1850, Germany). Transverse lumbar spinal cord cryosections were collected in serial at 30-μm or 40-μm thickness ...
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bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL) before immunostaining ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). The SARS-CoV-2 probe (cat ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted and snap-frozen on dry ice and sectioned at -20°C in 15 µm-thick coronal slices using a cryostat (Leica CM 1860 UV) and mounted onto Colorfrost Plus glass slides (Fisher 1255017) ...
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bioRxiv - Microbiology 2021Quote: ... Polymerization of these epoxy blocks was performed at 70 °C and then they were sectioned with an untramicrotome (Leica microsistems Gmbh, Wein Austria). Very small small sections of 80 nm thickness were collected on a cupper grid of 200 mesh (pore size ...
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bioRxiv - Neuroscience 2019Quote: ... brains were removed from the skull and 300μm-thick parasagittal slices (horizontal for thalamic stimulation experiments) were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Individual slices were then submerged in a recording chamber mounted on the stage of an upright BX51WI microscope (Olympus ...
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bioRxiv - Neuroscience 2019Quote: ... the neurons at the end of treatment were stained with JC-1probe solution at 37 °C for 20 min and observed under fluorescence microscope (DMi8, Leica Biosystems, Wetzlar, Germany). The mitochondria were also extracted by a mitochondrial extraction kit ...
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bioRxiv - Plant Biology 2019Quote: ... The abaxial side of the leaf samples was imaged using a 63×/1.2 water immersion objective lens (Leica C-APOCHROMAT 63×/1.2 water). FLIM experiments were performed using a Leica TCS SP8X confocal microscope equipped with TCSPC (time correlated single photon counting ...
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bioRxiv - Physiology 2019Quote: ... 5 mm of the mid-belly of lateral gastrocnemius muscle was cut into 10-μm thick frozen muscle cross-sections at −20°C with a cryostat (Leica, Wetzlar, CM1850, Germany). Because of the uneven thickness of the diaphragm ...
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Cell Biology 2022Quote: ... Vials were loosely capped and transferred to the chamber of the freeze substitution machine that contained about ½ inch-deep bath of ethanol to improve thermal conductivity and was pre-cooled to -112°C (EM AFSII, Leica Microsystems, Wetzlar, Germany). Within about 10 minutes in the freeze substitution machine ...
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bioRxiv - Immunology 2022Quote: Cryo-fluorescence microscopy samples were transferred to the cryo-FIB-SEM microscope in liquid nitrogen using a Leica EM-VCM500 machine and a cryo-holder suitable for c-clipped grids (Leica Microsystems, Vienna, Austria). Prior to sample loading into the FIB-SEM microscope ...
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bioRxiv - Neuroscience 2023Quote: ... for 48 hours at 4°C before being cut into 30 μm thick coronal serial sections on a sliding microtome (Leica Biosystems, Wetzlar Germany). The free-floating brain sections were then stored in antifreeze solution at −20°C until immunohistochemical staining.
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bioRxiv - Neuroscience 2023Quote: ... snap-frozen in powdered dry ice and stored at -80 °C until sectioned at 12 µm in the coronal plane with a Leica CM1860 UV cryostat (Leica Biosystems, Wetzlar, Germany). Sections corresponding to Atlas plates 20-26 of Paxinos (Bregma +1.34 to +0.62 mm ...
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bioRxiv - Genomics 2023Quote: ... and sliced into 13 µm slices using PTFE-coated microtome blades (Duraedge, 7223) at -10°C on a Cryostat CM1860 (Leica, 14-0491-46884). Liver sections were mounted on VWR Superfrost Plus slides (VWR ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were sectioned at 50 µm or 6 μm on a Leica CM3050 S cryostat set to −20°C for both the object and chamber temperature (Leica Biosystems, Nussloch, Germany). We collected sections from the primary somatosensory cortex (coronal sections from bregma ...
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Immunology 2024Quote: ... Brains were placed in 4 °C s-ACSF and sectioned with a vibrotome at 350–400 μm thick (Leica VT1200S; Leica, Nusslock, Germany). Collected slices were then incubated at 32 °C for 1 h in i-ACSF (124 mM/L NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Spheres were allowed to grow for 5 weeks and were photographed using an inverted bright-field microscope (Leica DMIRBE). Viable spheres were recovered by centrifugation at 100g for 5min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5-day old Arabidopsis seedling roots expressing AtC53-mCherry were cut and high-pressure frozen (EM PACT2, Leica, Germany), prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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Presynaptic mitochondria volume and abundance increase during development of a high-fidelity synapsebioRxiv - Neuroscience 2019Quote: ... Wafers were coated with a 5 nm layer of carbon before imaging to limit charging (Leica, Wetzlar, Germany, ACE600). Image acquisition was performed semi-automatically using a Merlin VP Compact SEM equipped with Atlas 5 AT software (Carl Zeiss Microscopy LLC. ...
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bioRxiv - Pathology 2020Quote: Frozen tissue blocks were sectioned at 5 µm thickness in a Leica CM1850 cryostat (Leica Microsystems GmbH, Wetzlar, Germany) at -20C ...
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bioRxiv - Developmental Biology 2021Quote: Live imaging of Rx3::H2B-GFP-derived organoids (Video 5 and 6) was performed with Sp8 confocal microscope (Leica). Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate ...
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bioRxiv - Zoology 2021Quote: ... Tissue sections with 5 μm thickness were cut using a microtome (Leica RM2235, Leica Biosystems Inc., Buffalo Grove, USA), fixed on slides ...
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bioRxiv - Cancer Biology 2022Quote: ... Invading cells were imaged with a sCMOS Orca-Flash 4.0LT camera (Hamamatsu) coupled 5 to an inverted DMi8 microscope (Leica), capturing serial z-stacks every 10 µm with a 10× objective (dry ACS APO 10x/NA 0.3) ...
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bioRxiv - Neuroscience 2021Quote: ... bubbled with 95% O2-5% CO2 and sliced using a Vibratome (Leica VT1200, Leica Microsystems Inc, Buffalo Grove IL) except for experiments in which high Ca2+ concentrations were used ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 100µm were performed at medium speed (around 5) and amplitude (approx. 6) using a vibratome Leica VT1000S (Leica). Sections were placed on a glass slide for immunostaining.
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bioRxiv - Neuroscience 2021Quote: ... The paraffin-embedded brains were sectioned in the coronal plane at 5 µm using a microtome (Leica RM 2265). Sections were collected at 40 μm intervals ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescence images of stained myotubes were obtained in 5 different areas using a DMI 3000 B microscope (Leica, Germany) and analyzed using ImageJ 1.52 software (National institutes of Health ...
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bioRxiv - Cell Biology 2019Quote: ... right after the processing and then sectioned at 5 µm by using Leica RM2255 (Leica Biosystems, Buffalo Grove, IL) for histological staining.
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bioRxiv - Physiology 2021Quote: ... Undecalcified 5 μm and 10 μm thick longitudinal sections were obtained using a microtome (RM2255, Leica Biosystems., IL, USA). 5 μm sections were stained with Goldner Trichome and at least two nonconsecutive sections per sample were examined for measurement of cellular parameters ...
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bioRxiv - Immunology 2021Quote: ... Organs included in paraffin were cut to a thickness of 5 microns (Microtome Leica RM2245 of disposable metal blades) and placed in a flotation solution in a water bath and then fixed on a slide sheet ...
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bioRxiv - Plant Biology 2020Quote: ... The embedded blocks were transversely sectioned at 5 to μm thickness using a Leica RM2125RT rotary microtome (Leica Microsystems) on RNase-free polyethylene naphthalate PEN membrane slides (Leica) ...