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Citations for Leica :
751 - 800 of 927 citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... mice were transcardially perfused with 10% formalin and coronal slices (100 μm) containing the hippocampus were cut with a vibratome (VT1000S, Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: The right hemisphere of each brain was sliced into 300μm coronal sections at −10°C using a cryostat (CM3050 S; Leica, Nuβloch, Germany). Punches of 0.75mm and 1.25mm were used to extract tissue from the frontal cortex ...
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bioRxiv - Bioengineering 2023Quote: ... was used to visualize the nano-architecture of PA hydrogel-stabilized cardiac tissue after decellularization (DECIPHER samples) and native cardiac tissue cryosections cut at 10 µm (Leica CM1950) from flash-frozen fresh young hearts embedded in OCT (Tissue-Tek ...
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bioRxiv - Neuroscience 2023Quote: ... compound (Sakura Finetek, CA, USA) and cryo-sectioned into 10 µm thick slices using a cryostat (CM1860; Leica Biosystems, Nussloch, Germany), The sections were mounted on glass slides ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue samples were frozen in OCT (Tissue-Tek) on dry ice and sectioned at 10 μm on a cryostat microtome (Leica CM1950). Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X- 100 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Mice were sacrificed by cervical dislocation and sagittal slices (250-400 μm) were prepared with an angle of 10-15° to maintain rostrocaudal striatonigral projections 82 using a vibrating microtome (Leica VT1200). Slice preparation was performed in ice-cold sucrose-based artificial cerebrospinal fluid (ASCF ...
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bioRxiv - Neuroscience 2024Quote: ... The immunofluorescence-stained brain sections were also imaged at ×10 and ×63 magnification using a Leica SP8 confocal microscope (Leica, Germany).
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bioRxiv - Biophysics 2024Quote: ... on top of which the samples (coverslips) were mounted using 10 μl of immersion oil (Type F immersion liquid, Leica Microsystems). After assembling the coverslip ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were then embedded in OCT media and cut into 10-μm-thick sections using a CM1900 Cryostat (Leica, Wetzlar, Germany). Primary human astrocytes were fixed with 4% PFA for 20 mins ...
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bioRxiv - Pathology 2019Quote: ... Secondary detection was performed this the Bond Polymer Refine Detection Kit (Leica, DS9800). Tissue sections were imaged using a Nikon Eclipse Ci light microscope with 4x or 20x objectives and Nikon Elements software.
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bioRxiv - Cancer Biology 2020Quote: ... Diaminobenzidine (DAB) staining was developed using the Bond Polymer Refine Detection Kit (Leica). Slides were counterstained with hematoxylin ...
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bioRxiv - Cancer Biology 2022Quote: ... blocking of endogenous peroxidase activity with Bond polymer refine detection kit (Leica: DS9800) (iii ...
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bioRxiv - Microbiology 2022Quote: ... Detection was completed using the Bond Research Detection kit (Leica Biosystems; CAT# DS9455). Nuclei were counterstained with DAPI (Vector Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... 306C7B3 stainings were done with enzyme pretreatment (Bond Enzyme Pretreatment Kit, Leica Biosystems) at 37°C for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... blocking of endogenous peroxidase activity with Bond polymer refine detection kit (Leica: DS9800) (iii ...
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bioRxiv - Cancer Biology 2023Quote: ... This was developed by application of the Novolink DAB substrate kit (Leica Biosystems) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... DAB refine and hematoxylin (Bond Polymer Refine Detection Kit, Leica Microsystems, Cat# DS9800) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 minutes using the Bond Polymer Refine Kit (Leica Biosystems, cat# DS9800) minus the DAB and Hematoxylin with a 1:150 dilution of OPAL520 reagent for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... was performed by the VCU Cancer Mouse Models Core Laboratory with the Leica Bond RX auto-stainer using heat-induced epitope retrieval buffer 1 (Leica, Sodium Citrate buffer, pH 6.0). Stained slides were then imaged on the Vectra Polaris (Akoya Biosciences) ...
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bioRxiv - Genetics 2021Quote: ... tissues were embedded in paraffin wax 60 (Sakura, Tokyo, Japan) and 10 μm sections were prepared using a Leica RM2125 microtome (Leica, Wetzlar, Germany). Sections were dried on glass slides overnight and then hydrated sequentially with 100% xylene ...
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bioRxiv - Neuroscience 2021Quote: ... The right hemisphere was cut into 40µm-thick sagittal sections in a series of 10 using a sliding microtome (Leica SM2010R, Wetzlar, Germany). The sections were stored in a cryoprotection solution at -20°C for further use ...
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bioRxiv - Molecular Biology 2021Quote: Frozen skin sections (thickness: 10 µm) from three Caucasian males were mounted on membrane slides (PEN-Membrane 2.0 µm, Leica Microsystems, Wetzlar, Germany) treated with 0.1 % (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
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bioRxiv - Bioengineering 2020Quote: ... and frozen to −50°C before cryosliced with a step of 10 μm at −20 °C using a modified cryotome (CM 1950; 839 Leica Microsystems, Germany). The slices were air-dried for 2 hours before staining ...
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bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Neuroscience 2020Quote: ... frozen with liquid nitrogen and cryo-sectioned (25 μm coronal section thickness for brains and 10 μm axial section thickness for spinal cords) using a cryostat (CM1850, Leica, Wetzlar, Germany) with a microtome blade (A35 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µm thick sections covering the injured area were produced using a cryostat maintained at -20°C (Leica CM 3050s, Techno-med). To assess the integrity of mitochondrial function the sections were sequentially stained for COX and SDH activities ...
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bioRxiv - Plant Biology 2021Quote: ... The percentage root colonization was obtained by analyzing 10 random stained roots under microscope at 20X (Leica Microscope. Type 020-518.500, Germany). Staining was performed by softening the sample with 10% KOH for 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... Transverse sections (10 µm thickness) were taken from the middle portion of kernels within the paraffin block on a manual microtome (Leica, Heidelberg, Germany). Sections were collected on Superfrost plus charged slides (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Spinal cord sections and longitudinal sciatic nerve sections were cut at 12 μm and DRGs at 10 um thickness using a cryostat (Leica Biosystems, CM3050S). Sciatic nerve and DRG sections were mounted on Superfrost+ microscope slides (Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... embedded in Tissue Tek® and after freezing at −80°C cut in 10 μm cross-sections using a Cryostat Microm HM560 (Leica Biosystems).
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bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were sputter-coated with a 10-nm-thick gold-palladium (80/20) layer using a high vacuum sputter coater Leica EM SCD500 (Leica Microsystems GmbH) and visualised with a Hitachi S-4800 field emission scanning electron microscope (Hitachi High-Technologies Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Plant Biology 2021Quote: ... five-day old seedlings were transferred to IAA containing liquid medium (10 µM) and imaged after 24 h (Leica TCS SP8 MP).
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bioRxiv - Cell Biology 2021Quote: ... Time-lapse confocal imaging (intervals of 10 seconds per image) was perfomed on a Leica SP5 confocal laser scanning microscope (Leica, Wetzlar, Germany) equipped with HCX PL APO λ blue 40.0×/1.25 oil objective ...
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bioRxiv - Systems Biology 2022Quote: ... were dropped under paraffin oil and their circumference measured by formula using the eye-piece graticule (10 mm = 200 parts) (Leica Microsystems, Germany). A 5μl drop was pulled around the midgut portion and MTs and a 0.3μl drop onto the CNC with the help of a capillary pull glass rod ...
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bioRxiv - Cancer Biology 2019Quote: ... before intracardial perfusion with heparinized 0.01 M PBS (10 U/ml of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Physiology 2021Quote: ... Diaphragm bundles were processed for immunohistochemical analyses of fiber cross-sectional area and myosin heavy chain isoforms as described previously.57,61 Bundles embedded in Tissue-Tek OCT freezing medium were sliced into sections of 10 μm thickness at approximately -20ºC using a cryostat (Leica, CM 3050S model). Sections were incubated in 1:200 wheat germ agglutinin (WGA ...
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bioRxiv - Cancer Biology 2021Quote: ... The prefrozen liver tissues in OCT were transverse sectioned at a thickness of 10 µm using a CM1950 cryostat (Leica, Wetzlar, Germany). Tissue sections adhering to the Stereo-seq chip surface were incubated at 37°C for 3 min ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).
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bioRxiv - Biophysics 2023Quote: ... and/or H2O2 (10 mM) were prepared immediately before measurements and condensates formation was monitored by DIC microscopy in situ (Leica LMD 7). Each sample was filled into 1.5 mm diameter quartz capillaries ...
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bioRxiv - Physiology 2023Quote: ... Cryosectioning of previously frozen placentas was performed with 10-mm-thick sections using a cryostat (Leica CM1860-UV, Leica Biosystems, Nussloch, Germany). Then ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Resin blocks were stored in −20°C until trimming into 10 µm sections with a RM 2255 microtome (Leica Biosystems, Nussloch, Germany). Sections were placed on adhesive glass slides (Menzel-Gläser Superfrost® Plus ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with calcein AM and Sir-Actin were captured at 10-s intervals for 12 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). During the imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Neuroscience 2023Quote: ... The cell volume of cultured ependymocytes were visualized by calcein signals at 10-s intervals for 12 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The cell volume from all calcein positive cells in a field of recording selected of every culture dish used in experiment were included in the statistics ...
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bioRxiv - Cancer Biology 2024Quote: ... The brains were cryoprotected using a sucrose gradient (10%, 20%, and 30%) and then coronally sectioned (30 μm thick) using a cryostat (Leica Microsystems, Germany). Three sections around the midpoint of the hippocampus were selected per animal for each stain ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were cut into 50 μm slices, images captured using Carl Zeiss Axio Scan microscope (Promenade 10, Jena, Germany) or confocal scanning microscope (DMi8, Leica, Wetzlar, Germany).