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Citations for Leica :
701 - 750 of 927 citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The fresh Phenol Red-free DMEM media with 10% FBS was added prior to confocal microscopic experiment (Leica Stellaris 8). A 405 nm laser excitation was used to image BFP and a 488 nm laser excitation was used to image EGFP.
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
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bioRxiv - Cancer Biology 2023Quote: ... snap-frozen livers were embedded in Tissue-Tek® O.C.T (Sakura: 4583) and cut in 10 μm sections using Leica Cryostat (Leica Biosystems). 5 sections in a distance of 100 μm to each other were stained per liver ...
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bioRxiv - Zoology 2023Quote: The histochemical investigation was conducted on tegumental enzymes using frozen sections prepared and cut to 10-12 µm in a cryostat (CM 3050S, Leica). The modified lead nitrate method detected acid phosphatase (AcPase ...
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bioRxiv - Developmental Biology 2023Quote: ... organoids were cryopreserved, embedded in OCT (Cell Path Ltd, Newtown, UK) and cut (10 µm) on a cryostat (Leica Cm1860). IF was performed as previously described [59] ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Neuroscience 2023Quote: ... Images were captured at 10-s intervals for 12 minutes by using a confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The cross-sectional area of central canal was normalized to the baseline area in 0 minute ...
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Glia detect and mount a protective response to loss of dendrite substructure integrity in C. elegansbioRxiv - Neuroscience 2023Quote: Animals were anesthetized on ice for ~10-60 minutes prior to observation with a fluorescence dissecting microscope equipped with a 2X objective (Leica). Animals with obvious GFP accumulation at the nose tip were scored as strong ...
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bioRxiv - Biochemistry 2024Quote: ... The grids were then blotted for 3.0 s at 10°C and 75% humidity and plunge-frozen in liquid ethane using an EMGP2 (Leica, Germany). Cryo-EM data were collected with a 300 kV Krios G4 transmission electron microscope (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... The number of neutrophils that migrated to the lower side of the membrane was counted in 10 random microscope fields using the x20 objective (Leica). Neutrophils that passed through the membrane were quantified ...
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bioRxiv - Neuroscience 2024Quote: ... The number of body bends per 20 seconds for at least 10 worms was recorded for worms in off-feed and on-feed conditions using stereo-zoom microscope (Leica)28 ...
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bioRxiv - Neuroscience 2024Quote: ... A segment from 12 mm to 17 mm from the lesion site was serially cut in 10 µm longitudinal sections in a cryostat (Leica) and picked up in glass slides ...
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bioRxiv - Molecular Biology 2021Quote: Facet joint cartilage and synovium (containing a thin layer of fibrous capsular tissue due to technical difficulties in obtaining pure synovium from mouse facet joints) were freshly dissected under a microscope (LEICA M80, with a LEICA IC80 HD video camera). Cartilage and synovium samples were collected separately in RNAlater solution (Ambion ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated with CD8 mouse monoclonal antibody (clone 4B11, 1:150, 0.2 µg IgG2b/ml) from Novocastra (Leica Microsystems, Newcastle Upon Tyne, UK) for 45 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections from human and mouse spinal cord or DRGs were cut at 12 μm using a freezing microtome (CM1850; Leica, Buffalo Grove, IL, USA) and incubated at 4°C overnight with primary antibodies including ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... Randomly selected hemispheres were sliced into serial sagittal plane sections with a thickness of 10 μm using a cryo-ultramicrotome (Leica, Germany), which were then stained by immunofluorescence.
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bioRxiv - Biophysics 2019Quote: ... Cryo-preserved skin in optimal cutting temperature (OCT) compound was sectioned to a nominal thickness of 10 µm (Leica CM3050 cryostat). DQ gelatin (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... The number of invading neutrophils was counted in 10 random fields of view using a fluorescence microscope (Leica SP8 Confocal microscope).
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bioRxiv - Neuroscience 2019Quote: ... mice (∼10 weeks of age) were sacrificed and coronal brain slices (300 µm thickness) were acquired with vibratome (LEICA VT 1200s) in ice-cold recovery solution (containing 93 mM NMDG ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were then rinsed with PBS five times (10 min each) and sectioned coronally at 60 μm using a Leica VT1000S vibratome (Leica, Germany).
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bioRxiv - Neuroscience 2020Quote: ... Transverse or longitudinal serial sections of 10 μm thick were made on a Reichert-Jung 23 Biocut microtome (Leica, Bannockburn, IL) of the scaffold and spinal cord areas.
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bioRxiv - Neuroscience 2021Quote: ... All slices were embedded in 10 % gelatin and further sectioned into slices of 60 μm thickness in ice-cold PB using a vibratome VT1000S (Leica Microsystems). After sectioning ...
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bioRxiv - Neuroscience 2021Quote: ... The frozen OCT block containing the organoid was then sectioned (10 µM thick) using a cryostat (Leica, Model CM 1950, Germany). For the immunocytochemistry staining ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Plant Biology 2021Quote: ... Samples were then mounted on aluminum stubs with carbon tape and sputter coated with 10 nm iridium (Leica EM ACE 600, Leica Microsystems) and imaged using a FEI Versa 3D scanning electron microscope (FEI ...
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bioRxiv - Neuroscience 2022Quote: ... 80% glycerol and imaged with the Leica TCS-SP2 confocal microscope using a 10x dry objective (HC PL APO CS 10×0.4, Leica, Bensheim, Germany), a 20x dry objective (HC PL APO CS 20.0×0.70 UV) ...
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bioRxiv - Bioengineering 2022Quote: ... The gels were subsequently incubated with EM containing 10 μM forskolin (Wako) with DMSO or Inh-172 for 24 h before imaging with a Leica DMi8 (Leica Microsystems) using the Leica Application Suite X (LAS-X ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were removed and placed in additional 10% formalin phosphate until sectioning into 40 µM thick sections via cryostat (CM 3050s; Leica Biosystems). Brain sections were stained with cresyl violet in order to visually confirm tetrode recording sites.
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bioRxiv - Systems Biology 2022Quote: ... followed by a blocking step for endogenous peroxidase using 0.3% H2O2/methanol and fixation with 10% neutral buffered formalin (Leica Biosystems, Germany). Slides were washed in Milli-Q water and 0.05% Tween20 in 1x Tris-Buffered Saline (TBS-T) ...
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bioRxiv - Cell Biology 2020Quote: ... CH) were carbon-coated to a thickness of 10 nm using the Leica EM ACE600 high-vacuum coating device (Leica Microsystems). The pattern of a Maxtaform H15 finder grid (Science Services ...
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bioRxiv - Physiology 2020Quote: Serial cross sections (10 μm) were cut from the TA and GA muscles using a Leica CM 3050S cryostat (Leica Microsystems). Muscle sections were fixed in cold acetone for 5 minutes at −20°C ...
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bioRxiv - Cell Biology 2021Quote: ... We used the optimized step intervals along the Z-axis to capture 3D images and the deconvolution of the acquired images was performed in the Leica Application Suite-X software using the tool blind deblur with 10 iterations (Leica Microsystems).
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bioRxiv - Cancer Biology 2021Quote: ... FFPE samples were sliced to a thickness of 10 μm and placed on a glass slide with foil (Leica, Wetzlar, Germany), stained with toluidine blue (see supplementary material ...
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bioRxiv - Biochemistry 2020Quote: ... H&E staining on 10 µm frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... samples were dehydrated in a graded ethanol series (50,70, 90, 100 and 100%) for 10 minutes each and then critical point dried (Leica CPD 300). Glass slips were adhered to a conductive carbon adhesive tab on aluminum stubs ...
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bioRxiv - Neuroscience 2020Quote: ... transverse hippocampal slices (400 μm) were prepared from male C57BL/6 mice (10-12 weeks of age) using a vibratome (Leica, VT1200S). The CA3 region was cut ...
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bioRxiv - Immunology 2022Quote: ... A total of 10 photomicrographs at 50x or 200x magnification per animal were randomly obtained using the microscope Leica DMI6000B (Leica microsystems). The total septal area and the total lung area were quantified using the Pro Plus 7 software (Media Cybernetics ...
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bioRxiv - Neuroscience 2022Quote: ... 10 control and 10 MK801-treated brains were cut to 10-μm thick frozen sections with a freezing microtome (Leica CM 1860 UV, Leica, Germany). The sections were stained with hematoxylin and eosin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse brain was embedded and frozen in optimal cut temperature (OCT) (SAKURA, USA) compound at - 80°C and 10 μm cryosection slices were prepared by using a Leica cryostat (Leica, Germany). The slices were warmed up to room temperature and fixed with 4% PFA for 5 min before used ...
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bioRxiv - Immunology 2022Quote: ... Four images of each well were taken with Leica DMi8 fluorescent microscope equipped with a 10× magnification objective (Leica, Wetzlar, Germany). Overall ...
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bioRxiv - Neuroscience 2022Quote: ... samples on SEM stubs were coated with a 10 or 15 nm platinum or gold layer using the sputter coating machine EM ACE600 (Leica Microsystems) at 30 mA current to be finally placed into the Crossbeam 540 FIB-SEM (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... H&E staining on 10 μM frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Microbiology 2023Quote: ... sectioned them at 10 µm thickness using a microtome and mounted the sections on thermoexitable POL-membranes (No. 115005191; Leica, Germany). Next ...
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bioRxiv - Neuroscience 2023Quote: ... using the same acquisition setting for all slices and at ×10 and ×63 magnification using a Leica SP8 confocal microscope (Leica, Germany). The images were analysed by a person blinded to the genotype using Qupath and ImageJ (NIH ...
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bioRxiv - Neuroscience 2023Quote: ... The paraffin-embedded brains and lumbar spinal cords were then serially sectioned at 10 μm thickness using a Leica RM2235 manual rotary microtome (Leica Biosystems) and mounted on poly-L-lysine microscope slides (ThermoFisher).
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bioRxiv - Cancer Biology 2023Quote: ... A microarray of fresh-frozen DMBA/TPA induced squamous cell carcinoma samples was cryo-sectioned with 10 μm thickness (Leica, CM3050S) and placed on the capture area of a spatial gene expression slide (10× Genomics ...
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bioRxiv - Developmental Biology 2022Quote: ... Processed samples were then embedded in OCT (Tissue-Tek: 4583) and transverse sections 10-14μm were taken using a cryostat (Leica: CM 3050S). EdU incorporation was visualized by using a labeling mix containing Copper Sulfate Pentahydrate (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...