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Citations for Leica :
601 - 650 of 927 citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and hematoxylin (Bond Polymer Refine Detection Kit, Leica Microsystems, Cat# DS9800) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the Bond Polymer Refine Detection Kit (DS9800, Leica). The slides were scanned with a whole-slide scanner (Aperio AT2 ...
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bioRxiv - Neuroscience 2022Quote: In vivo images of mouse retinal structure were obtained with spectral domain-optical coherence tomography (SD-OCT) (Leica Microsystems, Buffalo Grove, IL). The mice received 1% tropicamide drops to stimulate eye dilation and were anesthetized with ketamine and xylazine (50 and 5 mg/kg body weight ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121 ...
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bioRxiv - Neuroscience 2021Quote: ... and coronal sections of the mouse brain containing the dentate gyrus and prefrontal cortex were then cut using a VT1200S microslicer (300 μm) (Leica, Wetzlar, Germany). Micro-dissected areas were post-fixed at 4C for 2 hours in 2% osmium tetroxide in 0.1mol/l Cacodylate Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and incubated in blocking solution for 2.5h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The left and right hippocampus was micro dissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) then placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany) and secured with ear bars into a nose cone delivering isoflurane to maintain anesthesia ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2020Quote: ... At least 150 cells per mouse were examined and quantified for cell type following the manufacturer’s instructions using a CME microscope (Leica, Buffalo Grove, IL) with a 40x objective ...
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bioRxiv - Neuroscience 2023Quote: ... A single 250 μm-thick parasagittal slice was cut for each mouse in this ice-cold solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1000S; Leica, Deerfield, IL). Slices were then recovered at 32°C for 20-30 minutes in oxygenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) and placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany). Mice were given a subcutaneous injection of meloxicam (2 mg/kg ...
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bioRxiv - Cell Biology 2020Quote: Initial microscopic screening and quantitative examination of protein sorting in cultured mammalian cell lines was performed on a confocal Leica TCS SP5 II microscope (Leica GmbH, Heidelberg, Germany). Unless stated otherwise ...
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bioRxiv - Plant Biology 2019Quote: For BiFC assays Yellow Fluorescent Proteins (YFP) were visualized using a Leica TCS-SP2 laser scanning confocal microscope (LSM) (Leica Microsystems, Heidelberg, Germany) with a 63×1.4 NA oil-objective lens and processed using Leica confocal software v.2.61 about 16 h post-transformation ...
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bioRxiv - Cell Biology 2021Quote: ... the epithelium at ALI was visualized for Green Fluorescent Protein (GFP) at 20X by fluorescent microscopy using 3i Marianas/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA), assessed for functional phenotypes (as described below) ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections (10 μm) of inner ears were cut in the coronal plane using a cryostat (Leica CM-3050S cryostat). Whole mount brain slices were prepared from mice at P8 ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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bioRxiv - Neuroscience 2021Quote: ... Immunostained brain sections were analyzed with a ×10 objective using a Leica DMR microscope (DM6000B, Leica Microsystems, Wetzlar, Germany) equipped with a digital camera Leica DFX 3000FX (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 in 10 coronal sections (40 μm) were cut and collected using a Lecia CM1900 cyrostat (Leica Biosystems, UK) spanning the emergence of cortical M1 (approx ...
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20× ...
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bioRxiv - Neuroscience 2020Quote: ... Agarose blocks were then incubated for 10-15min on ice and sectioned using a vibratome (Leica VT100S vibrating microtome) in cold HBSS ...
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bioRxiv - Developmental Biology 2022Quote: ... Frozen sections with a thickness of 10 μm were prepared using a Leica CM1850 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were dried under an air dryer and kept at −80°C until use ...
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bioRxiv - Neuroscience 2022Quote: ... cerebral organoids were fixed in 4% PFA and sectioned into 10 µm thick slices with a cryostat (CM1950, Leica). Images were captured via a Leica TCS SP8 STED confocal microscope equipped with a LAS X software ...
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bioRxiv - Microbiology 2022Quote: Organ samples frozen in OCT were cut into 10 µm sections using a CM1850UV cryostat (Leica Biosystems, Wetzlar, Germany) and adhered to polysine microscopy slides (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... UK) which had been glow discharged for 20 seconds at 10 mA in an Ace 200 (Leica Microsystems (UK) Ltd ...
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bioRxiv - Cancer Biology 2021Quote: ... Images were acquired as three-dimensional scans with 10 μm Z-steps and processed with LAS X software (Leica) to obtain maximum projection images ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissue blocks were cut into 10 μm thick sections and mounted on membrane slides (PEN Membrane, 2.0 µm, Leica). LMD was conducted similarly for mouse and human samples ...
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bioRxiv - Cell Biology 2020Quote: 10 μL of the nuclei solution was pipetted on a hemocytometer (Neubauer) for visualization under the (Leica DM IL inverted) microscope ...
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bioRxiv - Cancer Biology 2020Quote: ... Bright field images were captured every 10 min for 72 h using an inverted motorized microscope (Leica DMI 6000) coupled with an incubation system to control the temperature and CO2 levels during the course of the experiments ...
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bioRxiv - Physiology 2021Quote: ... Undecalcified 5 μm and 10 μm thick longitudinal sections were obtained using a microtome (RM2255, Leica Biosystems., IL, USA). 5 μm sections were stained with Goldner Trichome and at least two nonconsecutive sections per sample were examined for measurement of cellular parameters ...
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bioRxiv - Plant Biology 2020Quote: ... They were coated with a 10 nm gold layer in an EM SCD005 sputter coater (Leica Microsystems, Vienna, Austria) and mounted on the microtome of the VolumeScope SEM (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The tissue blocks were sectioned by cryotome into 170 - 210 consecutive 10 µm thin tissue sections (1.7 - 2.1 mm total depth) which were placed on PEN membrane slides (Leica Microsystems). Representative sections were mounted on charged glass slides and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2020Quote: ... At least 5-10 flies per genotype were scanned using the Confocal Laser Scanning Platform Leica SP8 (Leica, Germany). Samples were excited with an argon laser at 488nm (20% tube current) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cryosections of 10 µm thickness were cut from snap-frozen samples using a cryomicrotome (CM1950; Leica Biosystems, Wetzlar, Germany). Cryosectioned samples were used for tissue staining and stored at -20°C ...
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bioRxiv - Neuroscience 2020Quote: ... 10–30 serial ultrathin sections (55 ± 5nm) were cut using a Leica Ultracut S ultramicrotome (Leica Microsystems, Vienna, Austria) and collected on Formvar-coated slot copper grids ...
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bioRxiv - Molecular Biology 2022Quote: ... Frozen muscles were transversely cryosectioned into 10 µM thick sections at the mid-belly on a cryostat (Leica Biosystems). Sections were stained with haematoxylin (H&E) ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen tissues were sliced coronally into 7 - 10 μm thick sections using a cryostat machine (Leica CM3050 S, Germany), mounted on glass slides and stored at −20 °C.
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bioRxiv - Microbiology 2023Quote: ... 10 and 24 h) using a Lumix DC T290 camera (Panasonic) equipped with a DC Vario-ELMAR lens (Leica). The area covered by bacterial cells at a given timepoint as well as the area of the whole agar plate were measured using Fiji ImageJ 1.52p ...
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bioRxiv - Neuroscience 2023Quote: ... Z-stacks were collected with a step size of 0.2 µm and deconvolved using the LAS AF One Software Blind algorithm (10 iterations) from Leica Software v3.1.0 ...
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bioRxiv - Plant Biology 2023Quote: ... Grids were blotted for 2.5 s at 10 °C and 95 % humidity using a Leica EM GP plunger (Leica). Data for NCPs was acquired at the Cryo-EM facility of the Gene Centre in Munich ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μm thick cryosections of the cerebral cortex and hippocampus were prepared using a vibratome (Leica VT1200, Wetzlar, Germany). The sections were Hoechst-stained ...
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bioRxiv - Bioengineering 2023Quote: ... was performed before coating of the samples with a 10 nm gold/palladium layer (Sputter Coater EM SCD500, Leica). Scanning electron microscopy was performed in a high vacuum environment at 10 kV voltage (Quattro S ...
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bioRxiv - Biophysics 2023Quote: Tiled images were captured using a Leica TCS SP8 laser scanning confocal microscope with a 20× oil immersion (NA 0.75) or a 10× air (NA 0.3) objective (Leica Microsystems). Images were merged and processed using LAS X (Leica Microsystems ...
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bioRxiv - Molecular Biology 2023Quote: ... Inhibitor-induced effects on worm viability were assessed using a stereo microscope at 10× magnification (M125 C, Leica, Germany) and the following scores ...
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bioRxiv - Cell Biology 2019Quote: The gastrocnemius and quadriceps muscles were isolated from mouse hind limbs and dehydrated in a 30% sucrose solution and then sectioned in a constant cold section box (Leica CM 3000, Germany) at −20 °C ...
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bioRxiv - Physiology 2021Quote: ... blocked samples were incubated for 1 hr at room temp in buffer A containing mouse anti-dystrophin IgG1 (1:20, #NCL-DYS2, Novocastra / Leica, Buffalo Grove, IL). The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500 ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...