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Citations for Tocris :
1401 - 1444 of 1444 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Cells grown in normal culture media supplemented with 10 ng/ml doxycycline for 48 hours to induce shRNA or treated with 1 μM TCE5003 (Tocris, 5099) for 22 hours ...
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bioRxiv - Neuroscience 2023Quote: iPSCs were replated at a density of 2 x 104 cells/cm2 in mTeSR medium containing 10 µM Rho kinase inhibitor (Y-27632, Tocris #5849), which was removed after 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... all buffers and solutions used during the process (from animal perfusion to sorted cells flash freezing) were supplemented with a cocktail composed of Actinomycin D (3μM, Tocris #1229/10), Anisomycin (100 μM ...
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bioRxiv - Microbiology 2024Quote: ... Samples were prepared in an aqueous buffer (150 mM NaCl, 10 mM HEPES) and incubated with either dextran sulfate (>500 kDa, MilliporeSigma # D6001) or heparin (Tocris # 2812) prepared in ultrapure water ...
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bioRxiv - Cell Biology 2024Quote: ... One million cells were plated per well of a Geltrex-coated 6-well plate in E6 medium (Thermo A1516401) supplemented with 10 μM ROCK inhibitor Y-27632 (Tocris 1254).
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bioRxiv - Neuroscience 2021Quote: ... The bath solution usually contained 6,7-dinitroquinoxaline-2,3-dione (DNQX, 10 µM) and D- aminophosphonovaleric acid (D-APV, 50 µM) to block ionotropic glutamate receptors (Tocris, Abingdon, United Kingdom). Patch electrodes had resistances of 4-6 MΩ when filled with KMeSO4- or CMeSO4-based internal solution (same as used in vivo) ...
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bioRxiv - Molecular Biology 2021Quote: ... the uptake of 10 μM of 14C-Glutamate was measured with or without the indicated concentrations of WAY213613 (Tocris, Bio-Techne, US). Expression of EAAT2 in total membranes was analyzed by immunoblotting ...
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bioRxiv - Neuroscience 2020Quote: ... GABA currents (IGABA) were elicited in one of two ways: pressure application of a GABA solution (10 μM in aCSF; Tocris #5A/205401) using a Picospritzer II (General Valve Corporation) ...
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bioRxiv - Neuroscience 2021Quote: ... Whole cell currents were recorded (see electrophysiology below) with synaptic transmission and action potentials blocked by CPPene (10 µM, Tocris bioscience; 1265), NBQX (10 μM ...
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bioRxiv - Neuroscience 2021Quote: ... which is a 1:1 combination of SKSRM and neuronal maintenance medium (NMM) supplemented with 10 μM SB431542 and 1 μM dorsomorphin (both from Tocris Bioscience Inc) for 10 days for neuronal induction ...
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bioRxiv - Microbiology 2021Quote: ... iAT2 cells were cultured as 2D cultures on Matrigel-coated plates in CK+DCI medium + 10 µM Y-27632 (Tocris, Cat#1254) for 48 h before switching to CK+DCI medium on day 3.
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bioRxiv - Neuroscience 2020Quote: ... To ensure that we recorded AP-driven neurotransmitter release a portion of the experiments were concluded by bath application of 10 μM tetrodotoxin (TTX, Tocris, Bristol, UK).
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bioRxiv - Biophysics 2022Quote: ... or αCD45 Fab (prepared as in previous work)8 was added in a 1:10 molar ratio to the desired dye - PAJF549 (Tocris Bioscience, UK) or HM-SiR (Sarafluor-650B ...
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bioRxiv - Genomics 2020Quote: ... supplemented with SMAD inhibitors (DMH-1 2 μM Tocris, Cat.: #4423; SB431542-10 μM Tocris, Cat.: #1614 and CHIR99021 3 μM, CHIR Tocris, Cat.: #4423).
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bioRxiv - Microbiology 2021Quote: ... iATII cells were cultured as 2D cultures on Matrigel-coated plates in CK+DCI medium + 10 µM Y-27632 (Tocris, Cat#1254) for 48h before switching to CK+DCI medium on day 3.
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bioRxiv - Neuroscience 2023Quote: ... we recorded the firing properties of hM4Di-expressing neurons before and after applying 10 μM clozapine N-oxide (CNO, Tocris, Cat.#: 4936) (in ACSF).
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bioRxiv - Neuroscience 2023Quote: ... Transduced neurons were placed onto a custom-built live-imaging chamber and imaged in Tyrode’s solution supplemented with 10 μM cyanquixaline (CNQX, Tocris Bioscience, Cambridge, UK) and 50 μM 2-amino-5-phosphonopentanoic acid (D-AP5 ...
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bioRxiv - Neuroscience 2022Quote: ... these experiments were performed in the presence of AMPA and NMDA receptor blockade (10 µM NBQX and 50 µM D-AP5 respectively; Tocris, Bristol, UK). To isolate excitatory currents ...
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bioRxiv - Neuroscience 2022Quote: ... experiments were performed in the presence of GABAA and GABAB blockade (5 µM SR-95531 and 10 µM CGP-52432 respectively; Tocris, Bristol, UK).
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bioRxiv - Neuroscience 2024Quote: ... Cells in dissociation reagent were incubated for 15-20 min at 37οC to ensure dissociation of cell clusters before adding 5 mL of DMEM/F12 containing 0.04% BSA and 10% ROCK inhibitor Y-27632 (1/1000; Tocris; Cat. No. 1254), followed by resuspension by gentle pipetting ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were centrifuged at 400 rpm for 5 mins and resuspended in NBFAK medium supplemented with 10 μM Rho-associated kinase inhibitor Y-27632 (ROCKi, Tocris Bioscience, UK). Cells were counted and seeded at 20 000 cells per well (or as described in the text ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025], 250 nM Purmorphamine [Tocris, 4551] ...
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bioRxiv - Physiology 2023Quote: Mice of 20 weeks of aged were injected retro-orbitally with 10 mM L-NMMA (NG-methyl-L-arginine acetate salt, Tocris Bioscience, 0771) to reach a final systemic concentration of 10 µM ...
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bioRxiv - Cell Biology 2023Quote: ... and distributed to 6-well low-attachment plates containing primitive streak/embryoid body media (10 μM Y-27632 (Tocris/Biotehne, Minneapolis, MN), 3 ng/ml BMP4 (R&D/Biotechne ...
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bioRxiv - Neuroscience 2024Quote: ... the animals were imaged as described above and then injected intraperitonially with either MPEP hydrochloride (10 mg/kg; Tocris; dissolved in ddH2O), an mGluR5 antagonist ...
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bioRxiv - Developmental Biology 2024Quote: ... Between 1.5 × 106 and 3.0 × 106 cells were added to each well in 2 ml mTeSR medium with 10 μM Rho-kinase inhibitor Y-27632 dihydrochloride (Tocris, UK # 1254). To aggregate the cells into the microwells ...
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bioRxiv - Developmental Biology 2020Quote: ... 9 month old Tg(id1-CRM2:GFP) fish were bathed in 300 ml fresh fish water containing 600 µl of a 10 mM DMH1 (Tocris, Wiesbaden-Nordenstadt, Germany) stock solution (final concentration 20 µM ...
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bioRxiv - Neuroscience 2021Quote: ... namely the mitogen activated protein kinase kinase (MAPKK) inhibitor U0126 (50 μM) and the CaM kinase II (CaMKII) inhibitor KN-62 (10 μM) (Tocris Bioscience, United Kingdom). The inhibitors were added to the cultures thirty minutes prior to DOI treatment ...
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bioRxiv - Neuroscience 2021Quote: ... were dissolved in magnesium-free HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 μM; Nimodipine, Tocris, 0600, 1 μM; LY-367385, Sigma, L4420, 100 μM; MPEP, Tocris, 1212, 10 μM). Type I mGluR agonist DHPG (Tocris ...
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bioRxiv - Neuroscience 2022Quote: ... Disassociated cells were pre-plated as reported earlier20 at a density of 200,000 cells/cm2 supplemented with 10 μM Y- 27632 (catalog# 1254, Tocris Bioscience, Bristol, United Kingdom) on Matrigel-coated plates and differentiation started when confluent ...
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bioRxiv - Neuroscience 2022Quote: ... dissociated hiPSCs were plated at 1×104 cells/cm2 into 6-well plates in the presence of 10 µM ROCK inhibitor (Y-27632, Tocris Bioscience, Bristol, UK). Cortical and hypothalamic differentiation took place on a substrate of geltrex (1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was aspirated and the pellet re-suspended in fresh E8 medium supplemented with 10 µM ROCK inhibitor (Tocris R&D Systems). Re-suspended cells were seeded at a density between 7,500 – 12,500 cells / cm2 on fresh Matrigel-coated plates (Day – 3) ...
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bioRxiv - Cancer Biology 2023Quote: ... USA), nicotinamide (final concentration, 10 mmol/L) (MilliporeSigma, St. Louis, MO, USA), prostaglandin E2 (final concentration, 1 μmol/L) (Tocris Bioscience, Bristol, UK) B27 supplement (final concentration ...
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bioRxiv - Neuroscience 2023Quote: ... were dissolved in HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 µM; Nimodipine, Tocris, 0600, 1 µM; LY-367385, Sigma, L4420, 100 µM; MPEP, Tocris, 1212, 10 µM). Type I mGluR agonist DHPG (Tocris ...
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bioRxiv - Neuroscience 2024Quote: ... hiPSCs were plated in 6-well plates in Basal Neural Maintenance media (BNMM) (Suppl. Table 1) supplemented with 10 μM SB-431542 (Tocris, Bristol, United Kingdom) and 0.5 μM LDN-193189 (Tocris ...
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bioRxiv - Neuroscience 2024Quote: ... the saline in the chamber was substituted with a 10 µM solution (in 100 µL saline) of the NMDA receptor antagonist (R)-CPP (Tocris Bioscience; Bristol, UK). After incubation for 1 h at 38 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the saline in the chamber was substituted with a 10 µM solution (in 100 µL saline) of the AMPA receptor antagonist NBQX disodium salt (NBQX; Tocris Bioscience; Bristol, UK). After incubation for 1 h at 38 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1% Pen/Strep) +2iLIF (10 ng/ml leukemia inhibitory factor + 3 μM CHIR99021 + 1 μM PD0325901 from Tocris, Cat. # 4423 and 4192, respectively). The cells were grown on 0.1% gelatin-coated dishes and usually seeded at a density of 0.5–1.5 × 104/cm2 ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μM forskolin (solvent: DMSO, 063-02193, Fujifilm Wako Pure Chemicals, Osaka, Japan) and 1 μM LDN193189 (solvent: DMSO, 6053-10, Tocris Bioscience Bristol United Kingdom), with an induction period of 10 days.
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bioRxiv - Neuroscience 2023Quote: ... for 18-30 minutes and reseeding them into Matrigel (BD Bioscience #354277)-coated 6-well plates at 1 x 105 cells/cm2 in cortical differentiation medium containing 10 µM Rock-inhibitor (Tocris, Minneapolis, MN, USA #1254), which was removed after 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... FAST-STEM hiPSCs grown to 80% confluency were dissociated into single cells using TypLE for 5 minutes and seeded into the Aggrewell at a density of 1.2×105 cells per-well in 1 mL of mTesR Plus with 10 μg/mL ROCK Inhibitor Y-27632 (Tocris Bioscience, Bristol, UK; 1254) and 1% Penicillin-Streptomycin (Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the extracellular solution was supplemented by 10 µM NBQX (Biotechne, CAS 479347-86-9) to block AMPA receptors and 50 µM APV (Tocris, CAS 79055-68-8) to block NMDA receptors ...
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bioRxiv - Biophysics 2021Quote: ... microtubules were grown with 32 µM tetra-rhodamine-labeled tubulin (25% labeled) for 30 minutes at 35°C and stabilized with 10 µM Taxol (Tocris, Minneapolis, MN, USA; Cat. # 1097) in BBR80 buffer ...
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bioRxiv - Neuroscience 2021Quote: ... glutamate and GABA receptor blockers (10 μM NBQX, Tocris, UK, 1044; 5 μM CPP, Tocris, 0247; 5 μM SR-95531, Tocris, 1262; 1 μM CGP-55845, Tocris, 1248) were included in the recording solutions.