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Citations for Tocris :
1351 - 1400 of 1444 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Neuronal activity was manipulated either with field stimulation (100mA,10 Hz,600 AP) or 6 hours of 50 µM picrotoxin (PTX, Tocris) treatment 65 ...
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bioRxiv - Cell Biology 2023Quote: iPSCs were first differentiated into iAT2s and passaged as above in 3D cultures in iAT2 medium (CK+DCI) supplemented for the first 3 days after passaging with 10 uM Y-27632 (Tocris). To generate iAT1s ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614] ...
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bioRxiv - Biochemistry 2022Quote: ... hiPSCs were patterned for 21 days with a growth factor cocktail to promote differentiation toward ventral midbrain neuronal progenitor cells for 11 days (10 mM SB431542, Tocris; 100 nM LDN193189 ...
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bioRxiv - Developmental Biology 2023Quote: ... The average baseline EPSP was established with a minimum of 20 sweeps before adding glutamate receptor antagonists (10 μM NBQX (Tocris) and 10 μM CPP (Tocris) ...
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bioRxiv - Cancer Biology 2023Quote: ... also known as H-1152 dihydrochloride (CAS 451462-58-1), was Santa Cruz Biotechnology (Dallas, TX). Fasudil (Cat. # 0541/10) and hydroxyfasudil hydrochloride (Cat. #2415) were purchased from Tocris Bioscience (Bristol ...
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bioRxiv - Neuroscience 2023Quote: ... were obtained by holding the membrane potential at 0 mV and with the presence of NBQX (10 μM, 0373, Tocris) and CPP (20 μM ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded in 60 mm dishes coated with GFR Matrigel and incubated in mTESR1 with 10 μM Y-27632 Rock inhibitor (Tocris). At the time of transfection ...
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Sexual differences in neuronal and synaptic properties across subregions of the mouse insular cortexbioRxiv - Neuroscience 2023Quote: ... were recorded at −70 mV and isolated by using the GABAA receptor blocker gabazine 10 mM (SR 95531 hydrobromide; Tocris).
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bioRxiv - Cell Biology 2023Quote: ... MSD3 iPSCs were transduced with lentiviral constructs in mTeSR Plus medium containing 10 µM Y-27632 (ROCK inhibitor; Tocris, 1254) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Oxotremorine (10 µM), Riluzole hydrochloride (100 µM), Strychnine (10 µM), Tetrodotoxin (TTX, 1 μM) and ZD7288 (50 µM) were obtained from Tocris. Suramin (100 µM ...
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bioRxiv - Developmental Biology 2024Quote: Incretin mimetic drug treatments were performed by supplementing unmodified Arc-3 medium with Metformin hydrochloride at 10 µM (Tocris, 2864), Exendin-4 at 10 nM (Tocris ...
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bioRxiv - Neuroscience 2024Quote: ... were recorded at −70 mV and isolated by using the GABAA receptor blocker gabazine 10 mM (SR 95531 hydrobromide; Tocris).
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bioRxiv - Neuroscience 2024Quote: ... NN3053 hESCs were plated on Lam-inin-521 in mTeSR1 medium with 10 µM Rock inhibitor Y-27632 (Tocris Bioscience). The following day ...
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bioRxiv - Neuroscience 2024Quote: ... D1-like and D2-like receptors were blocked by applying SFK 83566 hydrobromide for 5 minutes in the bath at 10 µM (1586, Tocris) and sulpiride at 10 µM (S8010 ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s protocol in mTeSR PLUS was supplemented with penicillin/streptomycin and 10 µM ROCK inhibitor (Y-27632 Tocris, 1254). Three days later about 200 EB were transferred to a well of a 24-well ultra-low attachment plate (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... The drugs used were 10 µM para-Nitroblebbistatin (motorPharma, #DR-N-111) or 5 µM Y-27632 (Tocris Bioscience, #1254). As a control we used dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... The stimulation protocol consisted of a 60-s baseline followed by a 30-s pulse of 100 µl of glutamate 10 µM (Tocris), and a 150-s washout ...
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bioRxiv - Cancer Biology 2024Quote: ... PCTS and PCLS 8 mm in diameter were cultured in BioR plates in the Bioreactor and treated with sorafenib (10 µM – 20 µM) (Tocris), lenvatinib (0.25 µM – 1.0 µM ...
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bioRxiv - Biochemistry 2024Quote: ... A549 RIG-I−/− ACE2+ cells (80-90% confluent in 6-well plates) were transduced by adding 10 µg ml−1 Polybrene (Tocris) and recombinant lentivirus to a multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2024Quote: ... coated 6-well plates with 1-1.5 million cells per well in E8 with 10 μM ROCK inhibitor Y-27632 (Tocris 1254). At day 1 of differentiation medium was exchanged to induction medium (IM ...
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bioRxiv - Neuroscience 2021Quote: ... cells were whole-cell voltage-clamped at −40mV and recorded in the presence of the GABAAR antagonist Gabazine (10 μM, Tocris 1262). N-methyl-D-aspartic acid (NMDA ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSCs were seeded on Matrigel-coated plates or coverslips at a density of 1.1×105 cells/cm² in mTeSR1 medium supplemented with 10 μM of ROCK inhibitor Y-27632 (Ri; Tocris, ref. 1254). To induce MES differentiation ...
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bioRxiv - Cell Biology 2021Quote: ... the Versene solution was then aspirated and replaced with E8 + 10 nM Y-27632 dihydrochloride ROCK inhibitor (Tocris cat. no. 125410); hiPSC colonies were then gently detached mechanically using a cell scraper ...
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bioRxiv - Cell Biology 2021Quote: ... 4 × 106 cells were seeded in a 21 cm2 dish and after 24 hours stimulated for ten minutes with 10 μM DAMGO ([D-Ala2, N-MePhe4, Gly-ol]-enkephalin, Tocris 1171) in DMEM medium (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... neural differentiation was started by changing the culture media to neural induction media (NIM) which included 10 μM SB431542 (Tocris, 616461) and 100 nM LDN-193189 (Stemgent ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension using accutase and seeded at a density of 1.0 x 106 cells per well on Matrigel coated 6-well plates in mTeSR1 supplemented with 10 mM ROCK inhibitor Y-27632 (Tocris, 1254).
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bioRxiv - Neuroscience 2021Quote: ... the Versene solution was then aspirated and replaced with E8 + 10 nM Y-27632 dihydrochloride ROCK inhibitor (Tocris cat. no. 125410); hiPSC colonies were then gently detached mechanically using a cell scraper ...
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bioRxiv - Neuroscience 2020Quote: ... hiPSC were passaged upon reaching approximately 80% confluence using EDTA at 37°C for three to five minutes and plated in mTESR1 with 10 μM Y-27562 (Tocris, UK). Media without Y-27562 was supplemented the day following.
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bioRxiv - Neuroscience 2022Quote: Macrophages were plated in a 12-well plate at 106 cells per well and treated with 10 μM E64 (Tocris, 5208) or 20 μM Z-Phe-Ala-FMK cathepsin B inhibitor (Santa Cruz ...
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bioRxiv - Neuroscience 2022Quote: ... and singularized cells were seeded at a defined density (between 42,000 and 62,000 cells/cm2 depending on the hiPSC line) on matrigel coated 6-well plates in StemMACS iPS-Brew supplemented with 10 µM Y27632 (ROCK inhibitor, Tocris Bioscience). After 24 h (Fig ...
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bioRxiv - Genetics 2021Quote: ... 4-6 x 104 cells per well were seeded onto V-bottom 96 well plate in NPC differentiation media suppled with 10 µM Y27632 (Tocris; 1254) to generate embryonic bodies (EBs ...
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bioRxiv - Cell Biology 2020Quote: ... into fibronectin-coated wells of two 96 well plates per biological replicate to assess proliferation on day 0 (D0) and day 3 (D3) in the presence or absence of 10 μM RARβ antagonist LE135 (Tocris Bioscience) using the cell viability MTS assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coated 6-well plates at a density of 100,000 cells/cm2 in E8 medium containing 10 μM ROCK-inhibitor (Y-27632 (Tocris, Bristol, UK)) ...
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bioRxiv - Cell Biology 2022Quote: ... Human ePSCs were thawed at 37°C and plated on top of the feeder cells in EPSCM with 10 µM Y-27632 dihydrochloride (Tocris, 1254) for 48 h ...
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bioRxiv - Genetics 2022Quote: ... Medium was changed on the next day and GFP positive cells were sorted 48-72 h post transfection and plated on feeder cells in standard TSC medium containing 10 μM ROCK inhibitor Y-27632 (Tocris #1254). Clonal TSC colonies were picked 7-10 days post sort ...
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bioRxiv - Genetics 2022Quote: ... cells were seeded in a 96-well plate on a feeder layer at a low density (~ 1 cell per well) in XEN media supplemented with 10 μM ROCK inhibitor Y-27632 (Tocris #1254) until clones appeared (approximately 5 days) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 200 μm CNQX and 10 μm CPP in some experiments to block all transmission to RGCs (Tocris, Bristol UK). All of the multi-electrode array recordings were processed with the software spyking-circus [Yger et al ...
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bioRxiv - Neuroscience 2022Quote: ... or 26-28hpl (late L1) were mounted on 10% agarose pads and immobilized in a pool of 3μL of 100 mM muscimol (TOCRIS biosciences #0289) + 7μL 0.05um polybeads (2.5% solids w/v ...
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bioRxiv - Neuroscience 2023Quote: ... 5-(4-Bromophenyl)-3-(1,2-dihydro-6-methyl-2-oxo-4-phenyl-3-quinolinyl)-4,5-dihydro-γ-oxo-1H-pyrazole-1-butanoic acid (DQP 1105, 10 μM dissolved in DMSO; Tocris Bioscience), or 6-Cyano-7-nitroquinoxaline-2,3-dione disodium (CNQX ...
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bioRxiv - Neuroscience 2022Quote: Cultured aNSCs/NPCs from young and old male C57BL/6 mice were plated onto PDL-coated coverslips in 24-well plates with complete activated media with or without 10 μM ROCK inhibitor (ROCKi) compound Y-27632 (dissolved in H2O) (Tocris, 1254). Forty-eight hours after plating ...
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bioRxiv - Neuroscience 2022Quote: aNSCs/NPCs were cultured in complete activated media until Passage 2-4 and then seeded at a density of 200,000 cells per well in a PDL-coated 12-well plate with complete activated media treated with vehicle (H2O) or 10 μM Y-27632 (dissolved in H2O) (Tocris, 1254). After 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 11320033))-coated coverslips in 24-well plates with complete activated or quiescent media with or without 10 μM Y-27632 (dissolved in H2O) (Tocris, 1254). After 48 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred to 96-well plates previously coated with fibronectin and containing 100 uL of 10% FBS DMEM culture media containing the WNT agonist CHIR99021 (Tocris, #4423), or antagonist XAV939 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... To enhance their neuronal fate NSC medium was enriched by 1 µM dorsomorphin (Stemgent, USA) and 10 µM SB-431542 (Tocris, UK). The selected neurorosettes were allowed to form neurospheres in T25 flasks ...
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bioRxiv - Genetics 2023Quote: ... and 10 µg of the Lenti guide puro/blast-gRNA plasmid using 1 mg/ml of PEI transfection reagent (Tocris, 7854). Incubation with the transfection mix was done for 5 hours at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... and then seeded at a density of 3.8 × 10⁴ cells per well in 1% Geltrex-coated 24-well cell culture plates (30024, SPL Life Sciences) in mTeSR1 supplemented with Y-27632 dihydrochloride (10 μM) (1254, Tocris Bioscience). The medium was replaced with mTeSR1 the following day ...
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bioRxiv - Genetics 2023Quote: ... and resuspended in stem cell media supplemented with 10 µM Y-27632 dihydrochloride (Rho kinase [ROCK] inhibitor; Tocris; cat. no. 1254). hiPSCs were used for ≤ 10 passages after thawing.
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bioRxiv - Genetics 2023Quote: ... and resuspended in stem cell media supplemented with 10 μM Y-27632 dihydrochloride (Rho kinase [ROCK] inhibitor; Tocris; cat. no. 1254). hiPSCs were used for no more than 10 passages after thawing ...
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bioRxiv - Developmental Biology 2023Quote: ... hPSCs were dissociated with EDTA for 10 minutes at 37°C and allowed to aggregate into spheroids of 10,000 cells each in V-bottom 96 well microplates (S-Bio) in E8 medium with ROCK inhibitor (Y-27632, 10 μM) and WNT inhibitor (XAV939, 5 μM, Tocris 3748). The next day (day 0) ...