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Citations for Tocris :
1301 - 1350 of 1444 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Final concentrations are indicated in parentheses: (+)-muscarine-iodide (10 μM) and SKF 81297 hydrobromide (1μM) were obtained from Tocris. FSK (50uM ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 million H9 or RUES2 hPSCs were plated in E8 medium with 10 μM Rock inhibitor Y-27632 (Tocris) in 1 well of 12-well culture plates coated with iMatrix-511 ...
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bioRxiv - Developmental Biology 2023Quote: ... For passaging TESR medium was further supplemented with 10 μM Rho kinase inhibitor Y-27632 (Tocris, Cat. No. 1254).
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bioRxiv - Bioengineering 2023Quote: ... 10 nM gastrin, 50 ng/ml recombinant human EGF (R&D, 236-EG) and 500 nM A83-01 (Tocris). Organoids were passaged every 4 days by physical dissociation using fire-polished Pasteur pipettes.
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated in Anteriorization media-1 (100 ng/ml Noggin (R&D/Biotechne) and 10 μM SB431542 (Tocris)) for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL culture was incubated with HADA at a final concentration of 10 µM (λem ≈ 450 nm, Tocris Bioscience) 19 for 10 min and washed 3X with anaerobic PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The cholinergic nature of these inputs was confirmed by application of 10 uM Dihydro-β-erythroidine hydrobromide (DhβE, Tocris) and 10 uM Methyllycaconitine (MLA ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were further digested into single cells in 0.2% trypsin in PBS supplemented with 10 µmol/mL Y-27632 (Tocris). The digestion was stopped with EDTA ...
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bioRxiv - Cell Biology 2024Quote: ... we generated frozen stocks of ROCKi-adapted iPSCs by expanding cells under constant exposure to 10 µM ROCKi (Tocris) in mTeSR medium ...
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bioRxiv - Physiology 2024Quote: ... Drugs were added from the following stock solutions: (2S,5R)-2-isopropyl-N-(4-methoxyphenyl)-5-methylcyclohexanecarboximide (WS-12, Tocris #3040, Bio-Techne Ltd, Abingdon, UK) 5 mM in ethanol ...
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bioRxiv - Cell Biology 2020Quote: ... Organoids were recovered by quick thaw in a 37C water bath followed by washing in organoid media and plating in ECM with organoid media plus 10 μM ROCK inhibitor Y-27632 (Tocris).
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bioRxiv - Cell Biology 2020Quote: ... Inhibition of voltage-gated L-type Ca2+ channels was done by introduction of 10 μM amlodipine besylate (Tocris, catalog # 2571) into the cell culture medium 30 min before the experiment ...
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bioRxiv - Genetics 2021Quote: ... which were resuspended in mTeSR Plus media with 10 µM Rho-associated protein kinase inhibitor (ROCKi, Tocris Bioscience #Y-27632) for 24 h ...
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bioRxiv - Developmental Biology 2021Quote: ... coated 12-well plates at the density of 6×105 cells/well in mTeSR1 medium supplemented with 10 μM ROCK inhibitor (Y-27632, Tocris). The medium was changed daily ...
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bioRxiv - Neuroscience 2021Quote: ... and GABA-A receptor blockers (10 μM GYKI 52466 cat #1454, 10 μM CPP cat #0247, 50 μM picrotoxin cat #1128, final concentrations, all from Tocris), with a bath perfusion rate of ∼2 ml/min ...
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bioRxiv - Neuroscience 2020Quote: ... and miniature IPSCs (mIPSCs) were recorded in presence of APV and NBQX (Tocris; 50 μM and 10 μM APV respectively) to block AMPAR and NMDAR currents ...
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bioRxiv - Genomics 2020Quote: ... The involvement of the TLR1/2 in signal transduction was tested using CU-CPT22 at 10 µM (Tocris Bioscience, UK). The used concentrations of inhibitors were selected based on recommendations provided by the manufacturers ...
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous activity was measured at either −60 mV (sPSCs, drug free) or +10 mV (GABAergic sPSCs, 100 μM CNQX, Tocris). Activity was recorded at DIV 28 ...
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bioRxiv - Neuroscience 2020Quote: ... In this method dual SMAD inhibition was performed using KOSR media supplemented with 10 μM ROCKi and SMAD inhibitors: 5 μM dorsomorphin (Tocris) and 10 μM SB431542 (Tocris ...
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bioRxiv - Neuroscience 2021Quote: ... Drug-evoked locomotor-like activities were induced by bath-applying 10 to 14 µM of N-methyl- D-aspartate (NMDA, Tocris) and serotonin (5-HT ...
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bioRxiv - Neuroscience 2020Quote: ... placed in an aCSF-filled borosilicate glass pipette (3-5 MΩ) in the presence of 6,7-Dinitroquinoxaline-2,3-dione (DNQX; 10 μM; Tocris #14A/166549).
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bioRxiv - Neuroscience 2021Quote: ... hiPSc colonies were dissociated and cultured as aggregates in suspension in iPSc media with 10 μM Y27632 (Tocris, Bristol, UK) and 4 ng/ml bFGF (Peprotech ...
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bioRxiv - Neuroscience 2020Quote: ... cells were rinsed and incubated for indicated times with fresh medium with either vehicle or 10 μM BAY 36-7620 (Tocris) in the presence or absence of lysosomal inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... animals were intraperitoneal (i.p.) injected (0.1 ml/ 10 g) 1 hour prior to baseline freezing assessment with norbinalthorphimine (norBNI, Tocris Cat.# 0347) diluted in sterile saline (1 mg/ ml ...
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bioRxiv - Molecular Biology 2020Quote: ... consisting of complete serum-free differentiation medium (cSFDM) base as previously described (Jacob et al., 2017) supplemented with 10 μm SB431542 (“SB”; Tocris) and 2 μm Dorsomorphin (“DS” ...
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bioRxiv - Neuroscience 2021Quote: ... C57BL/6 wild-type mice were daily treated with vehicle or clemastine (#1453 Tocris; 50mg/kg solved in 10% DMSO) by intraperitoneal injections from postnatal day 5 (P5 ...
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bioRxiv - Neuroscience 2021Quote: Whole-cell current clamp recordings of resting membrane potential and spontaneous firing were recorded in the presence of DNQX (10 μM; Tocris) and picrotoxin (100 μM ...
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bioRxiv - Neuroscience 2021Quote: ... dihydro-β-erythroidine hydrobromide (DHβE, 1 μM), nitrobenzylthioinosine (NBTI, 10 μM), and tetrodotoxin (TTX, 1 μM) were obtained from Tocris Bioscience ...
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bioRxiv - Neuroscience 2020Quote: ... Pluripotent hESCs were incubated with small molecules to start the dual SMAD inhibition: BMP and TGF-b receptors were pharmacologically blocked with 100 nM LDN193189 (Stemgent) and 10 mM SB431542 (Tocris), respectively ...
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bioRxiv - Bioengineering 2020Quote: ... Biolamina)-coated 384-well plates in 30 μL of E8 media supplemented with CEPT cocktail or Y-27632 (10 μM, Tocris). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were mixed with cathepsin-specific fluorescent substrate and optionally with 10 µM L 006235 or 1 µM pepstatin A (Tocris) and incubated for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the synaptic NMDARs thus activated were blocked by application of the open-channel irreversible antagonist MK-801 (10 µM, Tocris) in the continued presence of bicuculline for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 dissociated iPS cells resuspended in mTeSR1 media containing 10 µM Y-27362 dihydrochloride (ROCKi; Tocris Bioscience, cat. no. 1254) and 0.05% poly-vinyl alcohol (PVA ...
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bioRxiv - Cell Biology 2021Quote: ... The growth medium was supplemented with 100 ng/mL human recombinant epidermal growth factor (EGF) (R&D) as the major growth-supporting peptide and was further supplemented with 10 µM Y27632 (Tocris), 500 nM A83-01 (Tocris) ...
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bioRxiv - Neuroscience 2020Quote: ... During BQA experiments the ACSF solution contained the following substances: 10 μM D-(−)-2-Amino-5-phosphonopentanoic acid (D-AP5) (Tocris), 10 μM 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX ...
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bioRxiv - Neuroscience 2020Quote: Pharmacological experiments were carried out on NGFC-PC pairs using ACSF with the following drugs: 10 μM SR 95531 hydrobromide (Tocris), 10 μM D-(−)-2-Amino-5-phosphonopentanoic acid (D-AP5 ...
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bioRxiv - Cell Biology 2022Quote: Taxol microtubules were prepared as described above, however were labeled with 16% biotin and 5% Alexa555 tubulin and stored in BRB80T (BRB80, 10 μM Taxol (Tocris)) ...
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bioRxiv - Neuroscience 2022Quote: Primary microglia cells from C57Bl/6 J mice cultured in presence of CSF-1 were treated with Formoterol (10 ng/ml, cat # 1448, Tocris) or vehicle for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were placed on 10% agarose pads and immobilized using a combination of 3μL of 100mM muscimol (TOCRIS biosciences #0289) and 7μL 0.05um polybeads (2.5% solids w/v ...
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bioRxiv - Neuroscience 2022Quote: ... single worms were mounted on a 10% agarose pad with 2μL of anesthetic [from mixture of 3μL of 100mM muscimol (TOCRIS biosciences #0289) and 7μL 0.05um polybeads (2.5% solids w/v ...
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bioRxiv - Cell Biology 2022Quote: ... the actin depolymerizer latrunculin B (10 μM; for 30 min only to preserve cell viability; Tocris Bioscience; Thermo Fisher Scientific), or the YAP inhibitor verteporfin (0.5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... 150 μL of complete activated media (treated with vehicle (H2O) or 10 μM Y-27632 (dissolved in H2O) (Tocris, 1254)) was very carefully added to each well (for the ROCKi experiments) ...
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bioRxiv - Neuroscience 2024Quote: ... Accutase-dissociated single cells are FACS-sorted for the expression of the respective fluorescent marker protein and either directly used for bulk NGS based validation of the desired genome modification or subsequently plated at clonal density (250 to 350 cells/cm2) on MEFs in hESC media supplemented with 10 µm ROCK inhibitor (Y27632, ToCris) for the first 24 hr ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated and plated into pre-coated 6-well Aggrewell 800 plates at a concentration of 18M cells per well in hESC media with 10 µM Rock Inhibitor (ToCris). The next day (Day 1) ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated single cells were then washed in PXGL or UXGL medium and plated onto MEF feeder plates with PXGL or UXGL medium supplemented with ROCK inhibitor 10 µM Y27632 (Tocris), and then re-fed with PXGL or UXGL medium every 2 days ...
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bioRxiv - Bioengineering 2023Quote: ... The media were changed at day 3 to remove the ROCK inhibitor and to add 10 µM DAPT (Tocris Bioscience), which remained until day 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Male Sprague Dawley (SD) rats (6-10 weeks old) were administered with a single dose of Sugen 5416 (SU) (20mg/kg, Tocris) delivered in CMC vehicle (as previously described)16 or DMSO and placed in hypoxia chambers (10% O2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were routinely passaged at a 1:8-1:10 split ratio in the presence of Y-27632 (ROCK inhibitor, Tocris) for the first day after passaging and removed for subsequent media changes.
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bioRxiv - Neuroscience 2022Quote: ... All iPSC lines were first seeded concurrently on GeltrexTM (Thermo)-coated plates at 105,000 cells/cm2 (1,000,000 cells in a well of a six-well plate) in mTeSRTM medium supplemented with 10 μM Y-27632 (Tocris). The Y-27632 compound was removed from the medium the next day and subsequently the medium was replaced daily ...
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bioRxiv - Neuroscience 2022Quote: ... before changing to neural induction medium (NIM) which is neural maintenance medium (NMM) supplemented with 10 μM SB-431542 (Tocris) and 1 μM Dorsomorphin ...