Labshake search
Citations for Repligen :
101 - 142 of 142 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... EV-depleted FBS was collected from the filtrate from FBS introduced through tangential flow filtration (TFF) system with a 500 kDa molecular weight cutoff (MWCO) hollow fiber filter (polysulfone, Repligen, Waltham, MA). After 48 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein A agarose beads (RepliGen) were blocked for 1 hr at 4°C in Buffer BC150 containing 1% fish skin gelatine (Sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... Harvests were then concentrated using tangential flow filtration using a Minikros (100 kD) modified polyethersulfone (PES) hollow fiber filter (Repligen) on a KROSFLO KMPI system ...
-
bioRxiv - Immunology 2021Quote: Protein-A sepharose beads (Repligen, Waltham, Mass. USA) were covalently conjugated to 10 mg/mL W6/32 (pan-anti-HLA-I ...
-
bioRxiv - Bioengineering 2021Quote: ... Alternating tangential flow was set to 0.11 L/min (5 L/m2/min) using an XCell Lab Controller (Repligen). The desired rate of medium exchange was achieved by controlling the flow rate of the permeate (spent medium ...
-
bioRxiv - Bioengineering 2021Quote: ... A 0.022 m2 filter (ATF1, Repligen) used with alternating tangential flow at 5 L/m2/min to exchange medium at 3 L/day (0.11 L/m2/min ...
-
bioRxiv - Bioengineering 2021Quote: ... but with the addition of a KRJr permeate control pump (Repligen) set to 0.66 L/min/m2 ...
-
bioRxiv - Bioengineering 2021Quote: ... provides shear rate (1516 s−1) and other fluid dynamic parameters similar to those of an 11 m2 filter (ATF10, Repligen) used for perfusion of a 2000 L bioreactor of circa 1500 L working volume under recommended conditions ...
-
bioRxiv - Bioengineering 2021Quote: ... The submerged addition line was welded to the bioreactor connection line of an XCell ATF 1 single-use hollow-fibre filter and pump device (Repligen) with a 0.22 μm pore size ...
-
bioRxiv - Bioengineering 2021Quote: ... the clarified lysate was concentrated by tangential flow filtration at 5 L/min/m2 using a KR2i KrosFlo (Repligen) with Pellicon 2 mini cassettes (300 kDa ...
-
bioRxiv - Bioengineering 2021Quote: ... 8-10 kDa molecular weight cut off (MWCO) was purchased from Repligen (Waltham, MA, USA). TLR agonists (ssRNA40/LyoVec and CpG-A ODN 2216 ...
-
bioRxiv - Biophysics 2021Quote: ... using a 10 kDa MWCO dialysis membrane (Spectrum® Repligen, Waltham, MA, USA). For ACE2 purification ...
-
bioRxiv - Bioengineering 2021Quote: An alternating tangential flow filtration system (ATF 2, Repligen) was used for cell retention ...
-
bioRxiv - Bioengineering 2021Quote: ... an alternating tangential flow filtration system (ATF 2, Repligen) was utilized ...
-
bioRxiv - Microbiology 2021Quote: Particles in the flow-through from the filtration stage were concentrated on a 100 kDa tangential flow filter (Spectrumlabs, Repligen, Waltham, Massachusetts, USA) to a final volume of ~500 ml ...
-
bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dialyzed against 3 liters of Milli-Q water at 4°C using a 1 kDa cut-off dialysis tubing (Repligen Spectra/Por 6 Pre-Wetted Regenerated Cellulose ...
-
bioRxiv - Cell Biology 2021Quote: ... The pooled fractions containing the OAD were dialyzed (REPLIGEN dialysis membranes) against the OAD buffer for 6h at 4 °C to remove the sucrose and then loaded on an EnrichQ 5/50 column (Bio-Rad ...
-
bioRxiv - Synthetic Biology 2021Quote: ... the Avestin EmulsiFlex-C3 homogeniser was used at 15000-20000 psi and the final pellet-free cell extract was dialysed (using a Spectra/Por 4 Dialysis Tubing, 12-14 kD MWCO from Repligen) overnight in S30B buffer before one final centrifugation at 12000xg for 30 minutes ...
-
bioRxiv - Immunology 2021Quote: ... A final dialysis step was performed to place the product into the final formulation buffer using a 300 kD MWCO UF cartridge (Repligen mPES MiniKros, Cat No. S02-E300-05). The final purified SpFN 1B-06 PL recovery yielded 55.47 mg in a volume of 215 mL ...
-
bioRxiv - Physiology 2021Quote: ... The diluted LNP formulation was concentrated and diafiltered by tangential flow filtration using spectrum hollow fiber membranes (mPES Kros membranes, Repligen) and Tris buffer (pH 7.4 ...
-
bioRxiv - Bioengineering 2021Quote: ... Excess dye and/or drug was removed via KrosFlo II tangential flow filtration (TFF) system (Repligen, Waltham, MA). See supplemental method for cisplatin quantification techniques.
-
bioRxiv - Immunology 2021Quote: ... The culture in the bioreactor was cooled to 20°C before starting the concentration with a 790cm2 mPES hollow fiber with a 0.45µm pore size using the KMPi TFF system (Repligen). The concentrated biomass was diafiltrated with two Diafiltration Volumes (2DV ...
-
bioRxiv - Molecular Biology 2021Quote: ... The culture supernatant was applied to a column packed with CaptivA® Protein A Affinity Resin (Repligen, Massachusetts, USA) pre-equilibrated with 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 2 mM KH2PO4 pH 7.4 (PBS) ...
-
bioRxiv - Molecular Biology 2021Quote: ... rEGF (Repligen) and rTransferrin (Merck) ...
-
bioRxiv - Cell Biology 2020Quote: ... The streptavidin decoration of the cellulose membrane (Spectra/Por 7, Repligen, Waltham Massachusetts ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1x PIC) and pre-cleared for 1 h with either protein A agarose beads (Repligen, for RING1B ChIP) or protein A magnetic Dynabeads (Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... two pre-packed columns with POROS 50 HQ resin (0.8 cm ID x 5.0 cm H, V = 2.5 mL, strong anion exchange resin, Repligen GmBH) were first equilibrated with 5 column volumes (CV ...
-
bioRxiv - Bioengineering 2020Quote: Hollow fiber cartridges (molecular weight cut off, MWCO: 500 kDa, material: polysulfone) were supplied by Repligen (Rancho Dominguez, CA). Amicon® ultra-15 centrifugal filter units (MWCO ...
-
bioRxiv - Microbiology 2020Quote: ... Media dialysis was carried out using Spectra/Por®2 dialysis membrane tubing (29mm diameter) with a molecular weight cutoff of 12-14kD (Repligen, Waltham, Ma).
-
bioRxiv - Molecular Biology 2020Quote: ... Each aliquot was rotated for 1 hour with 100 μl 50% Sepharose Protein A Fast-Flow bead slurry (IPA400HC, Repligen) previously equilibrated in Buffer L ...
-
bioRxiv - Molecular Biology 2020Quote: Sonicated chromatin was precleared using Protein A agarose beads (Repligen) for 1 hour (1h ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein A beads (Repligen) were used to capture antibody-bound protein at 4 °C for 1 h ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates (500 µg for HEK293 or 2 mg for U251N) were incubated with 3 µg of indicated antibodies and 12 µl protein-A sepharose beads (IPA300, Repligen) (co-IP ...
-
bioRxiv - Immunology 2020Quote: Preparation of immunoaffinity columns: 1.5 mL of protein A agarose resin (Repligen) was washed (PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... Antibody-bound nucleosomes were captured for 1 hr at 4°C using protein A agarose (Repligen) beads ...
-
bioRxiv - Molecular Biology 2020Quote: ... ChIP reactions were incubated with pre-blocked protein A agarose beads (Repligen) for 2 hr (RING1B and SUZ12 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Diluted chromatin was pre-cleared for 1 hr using Protein A agarose beads (Repligen) that were pre-blocked with 1 mg/ ml BSA and 1 mg/ml yeast tRNA ...
-
bioRxiv - Immunology 2020Quote: ... Formulation was performed by TFF (ProStream mPES,1 m2 10 kDa MWCO, L-screen; Repligen) operated with a TMP of 15 psig at a process flux of 8 LMH ...
-
bioRxiv - Immunology 2020Quote: ... Harvest was performed via tangential flow filtration using 750 kDa hollow fiber cartridge (Spectrum/Repligen) at load challenge <50 g/m2 ...
-
bioRxiv - Cell Biology 2020Quote: ... 60 μL of Protein A agarose bead slurry (Repligen) were washed and resuspended in lysis buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein A agarose beads (Repligen) were used to capture the immunoprecipitated material for 1 h at 4°C ...
-
bioRxiv - Genetics 2020Quote: ... Vectors that encoded the anti-FAM19A5-IgG1 antibody were transfected into HEK293F cells and the recombinant antibody was purified using Protein A beads (RepliGen). Anti-FAM19A5 antibodies that recognized the epitopes formed at N-terminal and C-terminal regions were generated and called N-A5-Ab and C-A5-Ab ...