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Citations for Repligen :
51 - 100 of 118 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... The PET hydrolase reaction was induced on a cellulose dialysis membrane (Spectra/Por 2 Trial Kit, 12–14 kD: flat width 25 mm; Repligen, USA), and bottle-derived PET powder was used as the substrate ...
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bioRxiv - Bioengineering 2022Quote: ... and C02-E100-05-N tangential flow filtration filters were purchased from Repligen. Poly-L-arginine hydrochloride (38.5 kDa) ...
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bioRxiv - Bioengineering 2022Quote: ... Then the slurry was washed with 10x of 85 mM imidazole in PBS and the active protein was eluted with 250 mM imidazole and dialyzed three times against 100-fold excess of PBS at 4 °C (3,000 kDa molecular weight cut-off, Spectra/Por, Repligen). Finally ...
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bioRxiv - Bioengineering 2022Quote: ... MSC-conditioned media was collected after purified using sequential ultracentrifugation and tangential flow filtration (TFF) (KrosFlow KR2i, Repligen, Waltham, MA, USA). In brief ...
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bioRxiv - Bioengineering 2022Quote: ... The polymer was further purified using dialysis (1 kDa MWCO, Repligen) against 3.5 liters of deionized water with a total of four changes over 72 h at room temperature and lyophilized to obtain a pure white solid powder ...
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bioRxiv - Bioengineering 2022Quote: ... Thiolated dECM was purified by dialysis (100-500 Da MWCO, Repligen) against deionized water at room temperature and lyophilized to obtain a solid powder ...
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bioRxiv - Cell Biology 2022Quote: ... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
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bioRxiv - Cell Biology 2022Quote: ... followed by addition of Protein A agarose (Repligen Corporation, Waltham, MA) or protein G agarose (Thermo-Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... the organs were placed in a homemade filtered centrifuge tube with a nylon mesh filter with 20 μm opening pores (Repligen). The interstitial fluid (between 1-5 μL for each organ ...
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bioRxiv - Cancer Biology 2022Quote: ... The resultant liposomes were then cooled on ice for 5 minutes before dialysis using a 50kDa MWCO regenerated cellulose dialysis bag (Repligen, California, USA) overnight in pH 7.4 HBS at 4°C.
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bioRxiv - Cancer Biology 2022Quote: ... MAbs were purified using CaptivA® Protein A Affinity Resin (Repligen), for affinity chromatography ...
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bioRxiv - Immunology 2022Quote: ... followed by diafiltration and concentration through tangential flow filtration using a 750 kDa molecular weight cut-off (MWCO) modified polyethersulfone (mPES) membrane (Repligen, Waltham, MA). Terminal filtration of the saRNA material was done using a 0.22 µm PES filter ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μg of total brain or heart lysate extracts were incubated with 15 μL of Protein-A Sepharose beads (CaptivA protein resin, Repligen, Cat#CA- PRI-0100) and 2 μg of anti-Cav1.2 α1-subunit or control rabbit IgG antibody ...
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bioRxiv - Neuroscience 2022Quote: ... protein-A Agarose beads (Repligen 102500-03) and RFP trap magnetic beads (Chromo Tech rtma ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates clarified by centrifugation at 10,000g for 15 minutes at 4°C were incubated with 5ul of serum containing antibodies for 2 hours at 4°C followed by the addition of pre-blocked (with 1% BSA) Protein-A beads (Repligen) for an additional 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A-Sepharose was from Repligen (Needham, MA). Other common reagents were from Sigma-Aldrich.
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bioRxiv - Physiology 2022Quote: ... and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen). All proteins were further polished with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... the organs were placed in a homemade filtered centrifuge tube with a nylon mesh filter with 20 μm opening pores (Repligen). The interstitial fluid (between 1-5 μL for each organ ...
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bioRxiv - Biochemistry 2022Quote: ... An XCell™ ATF system (Repligen, Waltham, MA) was used for steady-state perfusion slowly ramping up the exchange rate from 0.25 to 1.0 vessel volumes a day (VVD ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Supernatants from each culture were dialyzed into 1.5 L 1X PBS for 24 hours using 6-8 kDa molecular weight cut off (MWCO) dialysis tubing (Repligen, #132655). Dialysed samples were collected and imidazole was added to a final concentration of 20 mM ...
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bioRxiv - Immunology 2021Quote: ... EV-depleted FBS was collected from the filtrate from FBS introduced through tangential flow filtration (TFF) system with a 500 kDa molecular weight cutoff (MWCO) hollow fiber filter (polysulfone, Repligen, Waltham, MA). After 48 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A agarose beads (RepliGen) were blocked for 1 hr at 4°C in Buffer BC150 containing 1% fish skin gelatine (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Harvests were then concentrated using tangential flow filtration using a Minikros (100 kD) modified polyethersulfone (PES) hollow fiber filter (Repligen) on a KROSFLO KMPI system ...
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bioRxiv - Immunology 2021Quote: Protein-A sepharose beads (Repligen, Waltham, Mass. USA) were covalently conjugated to 10 mg/mL W6/32 (pan-anti-HLA-I ...
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bioRxiv - Bioengineering 2021Quote: ... Alternating tangential flow was set to 0.11 L/min (5 L/m2/min) using an XCell Lab Controller (Repligen). The desired rate of medium exchange was achieved by controlling the flow rate of the permeate (spent medium ...
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bioRxiv - Bioengineering 2021Quote: ... A 0.022 m2 filter (ATF1, Repligen) used with alternating tangential flow at 5 L/m2/min to exchange medium at 3 L/day (0.11 L/m2/min ...
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bioRxiv - Bioengineering 2021Quote: ... but with the addition of a KRJr permeate control pump (Repligen) set to 0.66 L/min/m2 ...
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bioRxiv - Bioengineering 2021Quote: ... provides shear rate (1516 s−1) and other fluid dynamic parameters similar to those of an 11 m2 filter (ATF10, Repligen) used for perfusion of a 2000 L bioreactor of circa 1500 L working volume under recommended conditions ...
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bioRxiv - Bioengineering 2021Quote: ... The submerged addition line was welded to the bioreactor connection line of an XCell ATF 1 single-use hollow-fibre filter and pump device (Repligen) with a 0.22 μm pore size ...
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bioRxiv - Bioengineering 2021Quote: ... the clarified lysate was concentrated by tangential flow filtration at 5 L/min/m2 using a KR2i KrosFlo (Repligen) with Pellicon 2 mini cassettes (300 kDa ...
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bioRxiv - Bioengineering 2021Quote: ... 8-10 kDa molecular weight cut off (MWCO) was purchased from Repligen (Waltham, MA, USA). TLR agonists (ssRNA40/LyoVec and CpG-A ODN 2216 ...
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bioRxiv - Biophysics 2021Quote: ... using a 10 kDa MWCO dialysis membrane (Spectrum® Repligen, Waltham, MA, USA). For ACE2 purification ...
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bioRxiv - Bioengineering 2021Quote: An alternating tangential flow filtration system (ATF 2, Repligen) was used for cell retention ...
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bioRxiv - Bioengineering 2021Quote: ... an alternating tangential flow filtration system (ATF 2, Repligen) was utilized ...
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bioRxiv - Microbiology 2021Quote: Particles in the flow-through from the filtration stage were concentrated on a 100 kDa tangential flow filter (Spectrumlabs, Repligen, Waltham, Massachusetts, USA) to a final volume of ~500 ml ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dialyzed against 3 liters of Milli-Q water at 4°C using a 1 kDa cut-off dialysis tubing (Repligen Spectra/Por 6 Pre-Wetted Regenerated Cellulose ...
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bioRxiv - Cell Biology 2021Quote: ... The pooled fractions containing the OAD were dialyzed (REPLIGEN dialysis membranes) against the OAD buffer for 6h at 4 °C to remove the sucrose and then loaded on an EnrichQ 5/50 column (Bio-Rad ...
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bioRxiv - Synthetic Biology 2021Quote: ... the Avestin EmulsiFlex-C3 homogeniser was used at 15000-20000 psi and the final pellet-free cell extract was dialysed (using a Spectra/Por 4 Dialysis Tubing, 12-14 kD MWCO from Repligen) overnight in S30B buffer before one final centrifugation at 12000xg for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... A final dialysis step was performed to place the product into the final formulation buffer using a 300 kD MWCO UF cartridge (Repligen mPES MiniKros, Cat No. S02-E300-05). The final purified SpFN 1B-06 PL recovery yielded 55.47 mg in a volume of 215 mL ...
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bioRxiv - Physiology 2021Quote: ... The diluted LNP formulation was concentrated and diafiltered by tangential flow filtration using spectrum hollow fiber membranes (mPES Kros membranes, Repligen) and Tris buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2021Quote: ... Excess dye and/or drug was removed via KrosFlo II tangential flow filtration (TFF) system (Repligen, Waltham, MA). See supplemental method for cisplatin quantification techniques.
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bioRxiv - Immunology 2021Quote: ... The culture in the bioreactor was cooled to 20°C before starting the concentration with a 790cm2 mPES hollow fiber with a 0.45µm pore size using the KMPi TFF system (Repligen). The concentrated biomass was diafiltrated with two Diafiltration Volumes (2DV ...
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bioRxiv - Molecular Biology 2021Quote: ... The culture supernatant was applied to a column packed with CaptivA® Protein A Affinity Resin (Repligen, Massachusetts, USA) pre-equilibrated with 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 2 mM KH2PO4 pH 7.4 (PBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... rEGF (Repligen) and rTransferrin (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... The streptavidin decoration of the cellulose membrane (Spectra/Por 7, Repligen, Waltham Massachusetts ...
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bioRxiv - Molecular Biology 2020Quote: ... 1x PIC) and pre-cleared for 1 h with either protein A agarose beads (Repligen, for RING1B ChIP) or protein A magnetic Dynabeads (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... two pre-packed columns with POROS 50 HQ resin (0.8 cm ID x 5.0 cm H, V = 2.5 mL, strong anion exchange resin, Repligen GmBH) were first equilibrated with 5 column volumes (CV ...
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bioRxiv - Bioengineering 2020Quote: Hollow fiber cartridges (molecular weight cut off, MWCO: 500 kDa, material: polysulfone) were supplied by Repligen (Rancho Dominguez, CA). Amicon® ultra-15 centrifugal filter units (MWCO ...
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bioRxiv - Microbiology 2020Quote: ... Media dialysis was carried out using Spectra/Por®2 dialysis membrane tubing (29mm diameter) with a molecular weight cutoff of 12-14kD (Repligen, Waltham, Ma).
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bioRxiv - Molecular Biology 2020Quote: ... Each aliquot was rotated for 1 hour with 100 μl 50% Sepharose Protein A Fast-Flow bead slurry (IPA400HC, Repligen) previously equilibrated in Buffer L ...