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Citations for Repligen :
1 - 50 of 142 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 h incubation with 25 µL Protein A-agarose beads (RepliGen). Beads were washed five times with 0,1 x PBS after which beads were eluted in SDS sample buffer and incubated for 30 min at 37°C.
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bioRxiv - Biophysics 2024Quote: ... The dissolved peptide was added to a 1 mL Float-A-Lyzer G2 Dialysis Device from Repligen with a 0.5-1 kDa cutoff ...
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bioRxiv - Bioengineering 2024Quote: ... KrosFlo KR21 tangential flow filtration (TFF) equipment was purchased from Repligen (Waltham, MA). 96-well ...
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bioRxiv - Biophysics 2024Quote: ... (Repligen Spectra/Por, Spectrum Chemicals) and stored at 4° C.
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bioRxiv - Bioengineering 2024Quote: ... which used a polysulfone hollow fiber cartridges (Molecular weight cutoff: 300kDa, Repligen, Cat#D02-S500-05-N) as the filter ...
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bioRxiv - Bioengineering 2024Quote: ... LbL particles were then purified by TFF on a separate 100 kDa mPES membrane (Repligen) to remove any excess PLE.
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bioRxiv - Bioengineering 2024Quote: ... Excess dye was removed via extensive 0.9wt% NaCldialysis followed by extensive diH2O dialysis using a 3 kDa regenerated cellulose membrane (Repligen) and the purified PLE-cy3 was lyophilized until use.
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bioRxiv - Bioengineering 2024Quote: ... Excess PLR polymer was purified by TFF through a 100 kDa mPES membrane (Repligen) pre-treated with a 10 mg/mL solution of free PLR ...
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bioRxiv - Bioengineering 2024Quote: ... Ni-UL liposomes were purified via tangential flow filtration on a 100 kDa mPES membranes (Repligen) against 6 diafiltration volumes of deionized water.
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bioRxiv - Bioengineering 2024Quote: ... The resulting purified EVs were formulated in a sucrose-containing salt buffer via a second TFF diafiltration step (Repligen), then filter sterilized via 0.2 µm membrane (Sartorius ...
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bioRxiv - Evolutionary Biology 2024Quote: ... CaptivA® Protein A Affinity Resin (Repligen, CA-PRI-0025) (40 µl slurry per ChIP ...
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bioRxiv - Bioengineering 2024Quote: ... The clarified medium was concentrated up to 10-fold by volume by filtering through tangential flow filtration (TFF) (Repligen). After concentration ...
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bioRxiv - Immunology 2024Quote: ... 2 mg of anti-MHC-I mouse monoclonal antibodies (W6/32) covalently conjugated to Protein A sepharose (Repligen) using DMP as previously described [74,75] were added to the clarified supernatants and incubated with constant agitation for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 0.5% human serum albumin (Baxalta, NDC 0944-0493-01) and IGF-1 (0.1 µg/mL) (Repligen, 10-1011-125) for less than one day after isolation from the pancreas before being dissociated into single cells for LTC or scRNA-seq ...
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bioRxiv - Neuroscience 2024Quote: ... CNTNAP2- Fc secreted in the medium was affinity purified using ProteinA-CaptivA TM PriMAB (RepliGen) and subsequently cleaved with recombinant 3C protease to remove the Fc fragment and leave the mature (i.e. ...
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bioRxiv - Immunology 2024Quote: ... the sample was concentrated 20-fold on a 100 kDa hollow fiber TFF membrane (mPES, surface area: 0.16 m2, Repligen). After concentration ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 8G8-RSPO2RA SWEETS were purified using either CaptivA resin (Repligen, USA) under gravity-flow or using a prepacked MabSelect SuRe (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Ethanol was separated by tangential flow filtration (Repligen, TFF). The solution was concentrated to 0.1 mg/mL (mRNA concentration ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and purified using CaptivA® HF Protein A Resin (Repligen) in a gravity column ...
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bioRxiv - Biochemistry 2024Quote: ... or CaptivA Protein A sepharose (Repligen) plus the desired antibody ...
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bioRxiv - Biochemistry 2024Quote: ... or CaptivA Protein A sepharose (Repligen) plus the desired antibody ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3.5 kDa MWCO for release studies was obtained from Repligen (Massachusetts, USA). For Lipoplexes ...
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bioRxiv - Bioengineering 2024Quote: ... We installed a hollow-fiber membrane module (MiniKros Sampler 20 cm a 0.2 μm PES 1mm, Repligen GmbH, Ravensburg, Germany) to recycle the biomass back into the reactor ...
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bioRxiv - Synthetic Biology 2024Quote: ... filtrate was diafiltrated and concentrated to roughly 80 mL using the KrosFlo KR2i TFF System (Repligen, US) with 300 kDa cut-off hollow fiber filters (Spectrum Labs ...
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bioRxiv - Bioengineering 2024Quote: ... Dialysis tubing (Spectra/Por, 30 kDa) was purchased from Repligen (MA, USA), and CellBrite® Cytoplasmic Membrane Dyes were purchased from Biotium (Fremont ...
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bioRxiv - Immunology 2024Quote: RGFP966 (10 mg/kg, Repligen, Cat. #S7229) was dissolved in DMSO and diluted in a vehicle of 40% (vol/vol ...
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bioRxiv - Immunology 2024Quote: ... and elution steps were performed on an NGL COVID-19 resin (Repligen, Waltham, USA) as per the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... pH 5.2 and prepared at various concentrations from 85 mg/mL to 175mg/mL as measured in triplicate by Solo VPE (Repligen, Waltham Massachusetts). All viscosity measurements were performed at 20°C on an MCR-92 cone and plate rheometer (Anton Paar ...
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bioRxiv - Bioengineering 2024Quote: ... Tangential flow filtration filters were purchased from Repligen (Massachusetts, US).
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bioRxiv - Biophysics 2024Quote: ... and 300 kDa cut-off plugs (G235036, Repligen Corporation, CA USA) respectively following manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... Control and treatment samples were prepared in 3.5-5 kDa (G235029, Repligen Corporation, CA USA), and 300 kDa cut-off plugs (G235036 ...
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bioRxiv - Bioengineering 2024Quote: Nanoparticles were purified using a Spectrum Labs KrosFlo II filtration system (Repligen, Massachusetts, USA). Hollow fiber filter membranes with 100 or 300 kDa molecular weight cutoffs made of modified polyethylene sulfone were used to remove free dyes or polymers from the nanoparticle solution ...
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bioRxiv - Cancer Biology 2024Quote: ... was dialysed using the SpectraFlow Lab system (Repligen) and SnakeSkin dialysis tubing (3.5K MWCO ...
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bioRxiv - Cell Biology 2024Quote: ... using a 3.5 kD cutoff dialysis membrane (Repligen, 132724). The dialyzed proteins were run through a 5 mL HisTrap column to remove the cleaved His-ULP1 and the His-SUMO ...
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bioRxiv - Bioengineering 2024Quote: ... The 16,000 x g supernatants were filtered 0.2 µm and sEV isolated using a 300 kD MidiKros column (Repligen, Breda, Netherlands) on a KrosFlo Research IIi Tangential Flow Filtration (TFF ...
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bioRxiv - Bioengineering 2024Quote: ... on a KrosFlo Research IIi Tangential Flow Filtration (TFF) instrument (Repligen). The input flow rate was set to 50 mL/min resulting in a transmembrane pressure of approximately 300 mbars ...
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bioRxiv - Cell Biology 2024Quote: ... Hollow fiber columns with 300 kDa cut-off (MicroKros 20CM 300K MPES 0.5MM MLL X FLL 1/PK STER, Repligen) were used for TFF enrichment of EVs ...
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bioRxiv - Cancer Biology 2024Quote: Protein-A sepharose beads (Repligen, Waltham, Mass. USA) were covalently conjugated to 10 mg/mL W6/32 (pan-anti-HLA-I ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol and 2 mM dithiothreitol (DTT)) in 12-14 kDa Spectra/Por dialysis tubing (Repligen, cat#123697) with mild agitation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... using float-a-lyzer G2 dialysis cassettes with a MWCO of 8–10 Kd (Repligen, G235067), or buffer exchanged and concentrated in the same final formulation ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared conditioned tangential flow media was placed onto a 30 kDa cut-off filtration (TFF) column (Repligen, S02-E030-05-N, surface area 790 cm2). The media was concentrated 10-fold by placing the feed tube in the media and applying 12 PSI transmembrane pressure (TMP ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
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bioRxiv - Immunology 2023Quote: ... An alternate tangential flow system (ATF, Repligen) was used for perfusion ...
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bioRxiv - Bioengineering 2023Quote: ... We used tangential flow filtration (Krosflo KR2i; Repligen, Waltham, MA) to exchange ammonium sulfate for phosphate buffer solution (Potassium Phosphate Monobasic Anhydrous (KH2PO4 ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.70) using hollow fiber filter modules (100 kDa pore size, modified polyethersulfone; Spectrum MicroKros and MidiKros, Repligen). Liposomes were syringe filtered through 0.45 µm cellulose acetate membranes (VWR) ...
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bioRxiv - Cell Biology 2023Quote: ... Nanoparticles thus formed were dialyzed against HEPES buffer (pH 8.0) using dialysis tubing (Repligen, Waltham, USA) at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... and buffer exchanged into PBS by overnight dialysis through regenerated cellulose tubing (6-8 kDa MWCO, Repligen). To test conjugation ...
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bioRxiv - Cell Biology 2023Quote: ... chromatin was diluted to 300 µg/ml with lysis buffer and precleared with Protein A agarose beads (Repligen), blocked with BSA and tRNA ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant from the final centrifugation step was then passed through a 0.2 µm filter before subjection to tangential flow filtration (TFF) using a KrosFlo KR2i TFF system (Repligen; Boston, MA, USA). Using a protocol adapted from Heinemann et al. ...