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Citations for Repligen :
1 - 50 of 118 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 h incubation with 25 µL Protein A-agarose beads (RepliGen). Beads were washed five times with 0,1 x PBS after which beads were eluted in SDS sample buffer and incubated for 30 min at 37°C.
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bioRxiv - Bioengineering 2024Quote: ... KrosFlo KR21 tangential flow filtration (TFF) equipment was purchased from Repligen (Waltham, MA). 96-well ...
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bioRxiv - Biophysics 2024Quote: ... (Repligen Spectra/Por, Spectrum Chemicals) and stored at 4° C.
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bioRxiv - Microbiology 2024Quote: ... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The dissolved peptide was added to a 1 mL Float-A-Lyzer G2 Dialysis Device from Repligen with a 0.5-1 kDa cutoff ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was filtered by MICROKROS 20CM 0.65UM MPES (Repligen, and was concentrated by C06-E65U-07-S), MICROKROS 20CM 0.2 UM PES (Repligen ...
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bioRxiv - Bioengineering 2024Quote: ... and MICROKROS 20CM 0.05UM PS (Repligen, C02-S05U-05-S), respectively ...
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bioRxiv - Bioengineering 2024Quote: ... MICROKROS 20CM 0.2 UM PES (Repligen, C02-P20U-05-S), and MICROKROS 20CM 0.05UM PS (Repligen ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol and 2 mM dithiothreitol (DTT)) in 12-14 kDa Spectra/Por dialysis tubing (Repligen, cat#123697) with mild agitation at 4°C ...
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bioRxiv - Immunology 2023Quote: ... An alternate tangential flow system (ATF, Repligen) was used for perfusion ...
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bioRxiv - Bioengineering 2023Quote: ... We used tangential flow filtration (Krosflo KR2i; Repligen, Waltham, MA) to exchange ammonium sulfate for phosphate buffer solution (Potassium Phosphate Monobasic Anhydrous (KH2PO4 ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.70) using hollow fiber filter modules (100 kDa pore size, modified polyethersulfone; Spectrum MicroKros and MidiKros, Repligen). Liposomes were syringe filtered through 0.45 µm cellulose acetate membranes (VWR) ...
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bioRxiv - Cell Biology 2023Quote: ... Nanoparticles thus formed were dialyzed against HEPES buffer (pH 8.0) using dialysis tubing (Repligen, Waltham, USA) at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... and buffer exchanged into PBS by overnight dialysis through regenerated cellulose tubing (6-8 kDa MWCO, Repligen). To test conjugation ...
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bioRxiv - Cell Biology 2023Quote: ... chromatin was diluted to 300 µg/ml with lysis buffer and precleared with Protein A agarose beads (Repligen), blocked with BSA and tRNA ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant from the final centrifugation step was then passed through a 0.2 µm filter before subjection to tangential flow filtration (TFF) using a KrosFlo KR2i TFF system (Repligen; Boston, MA, USA). Using a protocol adapted from Heinemann et al. ...
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bioRxiv - Bioengineering 2023Quote: ... The product was then purified by dialysis in 1 kg/mol MWCO tubing (Repligen) against deionized water for three days ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02% sodium azide) using 6-8 kDa dialysis tubing (Repligen) at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02% sodium azide) overnight at 4C using 6-8 kDa molecular weight cut-off dialysis tubing (Repligen) and the expression tag was simultaneously removed using recombinant TEV protease (∼1:20 ratio mg/mg) ...
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bioRxiv - Biochemistry 2023Quote: ... An XCell™ ATF 2 stainless steel device (Repligen, Waltham, MA) with a 0.2 μm hollow filter fiber cartridge (Repligen ...
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bioRxiv - Biochemistry 2023Quote: ... with a 0.2 μm hollow filter fiber cartridge (Repligen, Waltham, MA) controlled by an XCell C24 Controller (Repligen ...
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bioRxiv - Biochemistry 2023Quote: ... controlled by an XCell C24 Controller (Repligen, Waltham, MA) was used for steady-state perfusion ...
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bioRxiv - Bioengineering 2023Quote: ... a 100-kDa MWCO MidiKros mPES membrane (D04-E100-05-N; Repligen, Boston, MA, USA) with 6 diafiltration steps and a transmembrane pressure of 5 PSI was used to concentrate samples to ∼10-15 mL26 ...
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bioRxiv - Bioengineering 2023Quote: ... or via tangential flow filtration (Repligen; KrosFlo Research I Peristaltic Pump with MicroKros Hollow Fiber Filter ...
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bioRxiv - Synthetic Biology 2023Quote: ... large volumes of conditioned media were diafiltrated and concentrated to roughly 50 mL using the KrosFlo KR2i TFF System (Repligen, US) with 300 kDa cut-off hollow fiber filters (Spetrum Labs ...
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bioRxiv - Bioengineering 2023Quote: ... Explanted retinas were positioned on a poly-lysine coated membrane (Sigma, P8920; Repligen 132544), with the RGCs side facing a 256-channel MEA (256MEA200/30iR-ITO ...
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bioRxiv - Bioengineering 2023Quote: ... Dialysis membranes (molecular weight cutoff [MWCO] value:12, 000–14, 000) were purchased from Repligen (USA). Liberase and DNase I were obtained from Merck (Darmstadt ...
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bioRxiv - Immunology 2023Quote: ... Spike protein from different variants were purified using a one-step affinity method with NGL COVID-19 Spike Protein Affinity Resin (Repligen, Waltham, MA, USA) (19) ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C followed by Protein A agarose beads (CA-PRI-0100, Repligen) for 1 hr the following morning ...
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bioRxiv - Bioengineering 2023Quote: ... 12-14 kDa molecular weight cut-off (MWCO) dialysis membrane was acquired from Repligen (Waltham, MA, USA). Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 8G8-RSPO2RA SWEETS were purified using either CaptivA resin (Repligen, USA) under gravity-flow or using a prepacked MabSelect SuRe (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... using float-a-lyzer G2 dialysis cassettes with a MWCO of 8–10 Kd (Repligen, G235067), or buffer exchanged and concentrated in the same final formulation ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared conditioned tangential flow media was placed onto a 30 kDa cut-off filtration (TFF) column (Repligen, S02-E030-05-N, surface area 790 cm2). The media was concentrated 10-fold by placing the feed tube in the media and applying 12 PSI transmembrane pressure (TMP ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Dialysis was performed against deionized water using Spectra/Por 3 dialysis membrane tubing (Repligen) with a MWCO of 3.5 kDa for 48 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A agarose beads (Repligen, 10-1003-02) or protein A/G magnetic beads (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A agarose (Repligen, #CA-PRI-0100), Mouse anti-HA magnetic beads (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... The suspension was then diafiltered using a MicroKros cross-flow diafiltration cartridge (500 kDa cutoff) from Repligen, Rancho Dominguez ...
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bioRxiv - Immunology 2022Quote: A 50 kDa cut-off Tangential flow filtration column (Repligen) was washed and equilibrated as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... SmT1v3-R and –D were purified using a one-step affinity method with NGL COVID-19 Spike Protein Affinity Resin (Repligen, Waltham, MA, USA). 150-250 ml of supernatant was applied to a 15-ml column by gravity flow ...
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bioRxiv - Immunology 2022Quote: ... using CaptivA™ PriMAB rProtein A Affinity Resin (Repligen). Resin was washed (50 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... antibodies were purified using the Protein A resin (Repligen, CA-PRI-0100). All the antibody preparations were reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen. Polystyrene semi-micro cuvettes for the Malvern Zetasizer were purchased from VWR and DTS1070 folded capillary cells were purchased directly from Malvern ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A agarose beads (Repligen) were blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA in 1x TE buffer at 4⁰C for 1 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A agarose beads (Repligen) were blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA in native ChIP incubation buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A beads (Repligen) were blocked in BC150 supplemented with 1% Fish gelatin (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mg of anti-MHC-I mouse monoclonal antibodies (W6/32) covalently conjugated to Protein A sepharose (Repligen) using DMP as previously described [42,43] were added to the clarified supernatants and incubated with constant agitation for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... resulting in a crude BTT(1,2)-DNA ligand.The BTT(1,2)-DNA ligand was purified using 3.5kD MWCO dialysis tubing for 24 hours in 3.5 L of water at room temperature (Repligen). Invitrogen Qubit ssDNA Assay Kit was used to measure the concentration of the purified BTT(1,2)-DNA ligand.
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bioRxiv - Microbiology 2022Quote: ... The filtrate was concentrated using a 100 kDa TFF column (Repligen N06-E100-05-N) and viruses were pelleted by ultracentrifugation (141,000 × g ...
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bioRxiv - Microbiology 2022Quote: ... TFF concentration (Repligen N06-E100-05-N, 100 kDa) optiprep (Sigma ...