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Citations for ChromoTek :
201 - 250 of 491 citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... A volume of 2 mL of total protein was mixed with 25 μL of GFP-TRAP agarose beads (50% slurry, ChromoTek) and incubated shaking overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and subjected to immunoprecipitation of RFP/mCherry/dsRed-tagged or GFP-tagged fusion proteins using RFP-trap or GFP-trap matrix (Chromotek), respectively ...
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bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mg of the total proteins were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA; Chromotek) for 2 hours at 4°C by vertical rotation ...
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bioRxiv - Plant Biology 2022Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... 600 μL of the supernatant were transferred to a 2.0 mL tube and an equal volume of protein extraction buffer with 5 μL GFP-Trap Magnetic Agarose beads (ChromoTek) were added ...
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bioRxiv - Neuroscience 2024Quote: ... for 1h at 4 °C to absorb bead-binding nonspecific proteins and immunoprecipitated using 50 μl of RFP-Trap magnetic Agarose (rtma, ChromoTek) for another 1h at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mg of protein lysate was incubated overnight at 4°C with 75 µl of GFP-Trap Agarose (gta-20, ChromoTek). For co- immunoprecipitations for MHC-I ...
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bioRxiv - Neuroscience 2020Quote: ... GFP fusion proteins in fixed cells were labelled with Alexa Fluor 647-coupled GFP nanobodies (GFP-booster gb2AF647-50, Chromotek, Germany) at 1:100 dilution ...
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bioRxiv - Microbiology 2022Quote: ... and 500 µg of protein from each sample was incubated with 35 µL pre-washed GFP-Trap magnetic beads (Chromotek, Inc.) for 1 h at 4 °C with rotation (1 mg/mL final lysate concentration) ...
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bioRxiv - Plant Biology 2021Quote: One milligram of total protein extract was mixed with 25 μl of GFP Nanobody/VHH coupled to agarose beads (GFP-Trap® Agarose, Chromotek) and incubated for 3 hours and 30 minutes at 4°C with end to end rocking ...
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bioRxiv - Cell Biology 2022Quote: ... Then 800-1000 µg of total protein lysates were added to 25 μl of anti-GFP magnetic beads (ChromoTek GFP-Trap) and tumbled end-over-end for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: For CoIP experiments 500 μl protein extract was mixed with 25 μl calibrated anti-GFP magnetic beads (ChromoTek GFP-Trap®) and incubated on a rotator for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Plant Biology 2020Quote: Blotted proteins were detected using anti-GFP (LifeTechnologies, Darmstadt, Germany) and anti-red FP (RFP) antibody (ChromoTek GmbH, Planegg-Martinsried, Germany) (both ...
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bioRxiv - Cell Biology 2023Quote: ... and approximately 250 µg of protein was incubated with anti-GFP-conjugated magnetic agarose beads (ChromoTek GFP-Trap® Magnetic Agarose) for 1-1.5 hours ...
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bioRxiv - Plant Biology 2024Quote: ... between EIN2-C and HAF2 following study previous briefly: Total protein extracts from plants mentioned in the paper were immunoprecipitated by DYKDDDDK Fab-Trap™ agarose beads (ChromoTek) for two hours at 4 °C with gentle rotating and then beads were washed five times with ice-cold 1XPBS buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Total proteins were extracted as described above and the extraction was mixed with 30 μL RFP-Trap® magnetic agarose beads (Chromotek) and rotated at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were adjusted to 0.5% NP-40 and 500mg of protein incubated with pre-washed magnetic GFP-TRAP beads (ChromoTek gtma-20) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... IPs of GFP-tagged proteins were performed by incubating cleared cell lysates for 2 hours at 4°C with GFP-Trap® beads (ChromoTek) that had been pre-equilibrated with cold IPB ...
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bioRxiv - Plant Biology 2024Quote: ... α-GFP (Chromotek, 3H9, 1:2000), α-YFP (Venus ...
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bioRxiv - Molecular Biology 2023Quote: ... mNeonGreen (1:500; Chromotek, 32f6). Coverslips were mounted using anti-fade reagent Prolong Gold containing DAPI (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... 1:400 GFP-booster (Chromotek), DAPI (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 100 mg of total protein (4 ml of extract) were immunoprecipitated with 25 µl of GFP-Trap magnetic agarose beads (Chromotek gtma-20) following incubation for 2 h at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... Twenty milligram protein extracts were taken as input and the rest of the extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates (200 μg protein) from HEK-293T cells expressing WT-TRPC6-YFP was incubated with GFP M-trap beads (Chromotek GTD-20) to immunoprecipitate TRPC6 ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mL of protein extract was incubated with 60 μL (50% slurry) of GFP-trap magnetic agarose beads (ChromoTek, Planegg-Martinsried, Germany) for 3 h on a rotary shaker at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-RFP (Invitrogen, #R10367, 1:1000 for WB and Chromotek #5F8, 1:1000 for WB), anti-phospho myosin light chain (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2022Quote: ... and 1:500 anti-rabbit nanobody coupled to Alexa Fluor 647 (Chromotek srbAF647-1-100). Slides were washed three times for two minutes in PBS in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti-RFP (1:20, Heinrich Leonhardt, 5F8) and rat anti-RFP (1:2000, ChromoTek-5F8). Fluorescent dyes DAPI (0.25 ng/µl ...
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bioRxiv - Plant Biology 2024Quote: ... membranes were probed with primary antibodies anti-GFP (1:1000) or anti-RFP (1:2000) (Chromotek) for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaves and pulldown assay were carried out according to102 with the following modifications: For immunoprecipitation of GFP-tagged proteins 50 µl of GFP-Trap magnetic beads (Chromotek, Planegg-Martinsried, Germany) were washed 3 times with extraction buffer and incubated for 2 h at 4 °C with 500 µl of a total protein extract containing a protease inhibitor cocktail (1x cOmplete™ ...
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bioRxiv - Plant Biology 2022Quote: ... or anti-RFP (1:2000; Chromotek) antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-booster (Chromotek; 1:300 dilution) was used to amplify GFP fluorescence for all tagged transgenes ...
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bioRxiv - Biochemistry 2021Quote: ... GFP-Booster_Atto488 (IF 1:300, ChromoTek), mouse anti-GFP (WB ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-Booster (1:1000; ABIN509419; Chromotek). The secondary antibodies used were goat anti-mouse IgG Alexa Fluor 568 conjugate (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... rat anti-RFP (1:1000, Chromotek), mouse anti-LN (1:10 ...
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bioRxiv - Cell Biology 2022Quote: ... rat anti-RFP (1:1000, Chromotek), mouse anti-Arl13B (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... GFP (1:5’000 Chromotek #3H9-100), GUS (1:1’000 Sigma Aldrich #G5545) ...
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bioRxiv - Neuroscience 2022Quote: ... anti-RFP (1:1000, rat, Chromotek), and anti-Lhx2 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-RFP (5f8, Chromotek, 1:500), and anti-Myc (sc-40 ...
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bioRxiv - Molecular Biology 2020Quote: ... PCNA (Chromotek, 16D10-100, 1:500). Secondary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... anti-Myc (ChromoTek, 1:1000, AB_2631398), anti-Vinculin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... anti-GFP (ChromoTek, 1:1000, 3H9), anti-RFP (ChromoTek ...
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bioRxiv - Biochemistry 2023Quote: ... anti-RFP (ChromoTek, 1:1000, 6G6). The secondary antibodies were ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GFP (1:1000, Chromotek, PABG1). Membranes were incubated for 1 h with either an IRDye secondary antibody (for LI-COR Odyssey imaging ...
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bioRxiv - Developmental Biology 2022Quote: ... rat anti-RFP (Chromotek, 1:200); mouse anti-β-Gal (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... anti-RFP (1:1000, rat, Chromotek), anti-KN (1:4000 ...