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Citations for ChromoTek :
451 - 491 of 491 citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 1% Triton X-100) and incubated with 250 µl of magnetic beads (RFP-Trap Dynabeads, Chromotek, rtd-20) for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL of lysate was added to 10 μl GFP-Trap® Magnetic Particles M-270 (Chromotek/ Proteintech) and incubated for 2h at 4°C on a rotating platform ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mg lysate per sample was subjected to immunoprecipitation overnight using GFP-Trap Magnetic Agarose (ChromoTek GmBh) at 4 ℃ on a rotary shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Samples (inputs and eluates) were further analysed by western blot with rat anti-GFP (1:1000, 3H9, Chromotek) and rabbit anti-Fbxo42 (1:1000 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 ml of filtered plant extract was incubated with 30 µl of GFP-Trap® Magnetic beads (Chromotek) in a rotatory mixer at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek). Beads were then washed thrice in buffer containing 50mM Tris-HCl pH=7.5 and 150mM NaCl buffer (no protease inhibitors or detergent) ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of lysate was incubated with 25 μL of equilibrated GFP-trap magnetic agarose beads (Chromotek, gtma-20) for 1 h with end-over-end rotation at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... one step of O/N incubation at 4ºC with GFP booster (1:250, Alexa Fluor ® 488 Chromotek #gb2AF488), Phalloidin-647 (1:250 ...
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bioRxiv - Neuroscience 2022Quote: ... Another set of sections was incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... mCherry and GFP or EYFP signals in the neurons were enhanced by immunostainings using the following antibodies: rat anti-RFP (1:1,000, Chromotek) revealed by Cy3-conjugated donkey anti-rat secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2021Quote: Single-molecule tracking was carried out in modified Tyrode’s solution supplement with 0.8% BSA and ATTO647N-conjugated anti-GFP nanobodies (1:15000 dilution, GFPBooster-ATTO647N, Chromotek, #gba647n) on Nanoimager microscope (Oxford Nanoimaging ...
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bioRxiv - Immunology 2021Quote: ... GFP-tagged PD-1 variants or BTLA variants were IP’ed from the lysate using GFP-Trap (Chromotek, #gta-20). Equal fractions of the IP samples were subjected to SDS-PAGE and blotted with indicated antibodies.
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bioRxiv - Developmental Biology 2021Quote: ... blocked as above and incubated in primary (rat anti-GFP, 1:200, ChromoTek, Cat. No. 3h9-100, clone 3H9) diluted in block as above ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was blocked using BlueBlock PF (SERVA) and developed using rat monoclonal anti-RFP 5F8 (1:2000, Chromotek) and rabbit anti-rat-peroxidase A9542 (1:20000 ...
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bioRxiv - Biophysics 2023Quote: ... 0.25 mM PMSF) and eluted with 4 × 1 mL 0.15 mg/mL (50 µM) 3 x FLAG peptide (Chromotek). The elutant was concentrated with Amico-4 (50k MWCO ...
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bioRxiv - Neuroscience 2022Quote: ... Sections ipsilateral to the injection side were incubated in a mixture of the following primary antibodies: rat-RFP (1:1,000, Chromotek), rabbit anti-somatostatin (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were washed extensively and GFP-booster (tagged with Alexa488, ChromoTek, gb2AF488-50, 1:500 in 3%BSA/PBS) and anti-rabbit-Alexa568 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: FLAG-Trap immunoprecipitations were performed in TREx-BCBL-1 RTA FLAG-ORF11 O/E cells as per the manufacturer’s protocol (Chromotek). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were blocked in 3% BSA in PBS-T for 30 min before incubation with GFP-Booster _Atto488 (1:300; Chromotek), or mouse anti-G3BP1 in 3% BSA in PBS-T for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Arabidopsis stable lines expressing native VAP27-1-RFP or Mito-mCherry were stained with anti-TraB1a mouse primary antibody at a dilution of 1:500 and anti-RFP rat primary antibody (Chromotek), followed by secondary antibody incubation with FITC conjugated against mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek, Germany, Cat. No. gtma-20) for 3h at 4°C while rotating ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: Samples for STED microscopy were prepared by incubating yeGFP-tagged Cdc3 cells (wild-type CDC11 or mutants) with a 1/250 dilution of an anti-GFP nanobody Atto 647N (ChromoTek), as previously detailed in (52) ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... resuspended in 1 ml ice cold IPP150 buffer and subsequently processed using similar protocol as above with the following exceptions: 1/ supernatant was incubated with 50 μl of V5-Trap magnetic Particles M-270 (Chromotek, v5td-20 ...
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bioRxiv - Molecular Biology 2022Quote: ... on a rotating wheel at 4° C for 1 h in triplicates with GFP-Trap agarose beads (#gta-200, Chromotek) or control agarose beads (Chromotek) ...
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bioRxiv - Cell Biology 2023Quote: ... portions of the blot were reacted against anti- mNeonGreen (mouse monoclonal antibody 32F6 from Chromotek, Inc., at 1:200 dilution), anti- HA (rabbit monoclonal antibody ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed with a buffer containing 1% Triton X-100 for 20 min and the lysate was incubated with anti-GFP magnetic beads (Chromotek) for 2 hours in 0.33% Triton X-100 solution ...
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bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...
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bioRxiv - Plant Biology 2024Quote: Immunoprecipitation was performed by adding 1 g of homogenized leaf material 50 µL of GFP-Trap® coupled to magnetic beads (ChromoTek). Samples were processed as described (Raffeiner et al. ...
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bioRxiv - Cell Biology 2023Quote: ... mEGFP-mAID-NIPBL and SCC1-mEGFP were detected and visualised with an anti-GFP nanobody booster (Chromotek, gba488-100, 1:250).
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bioRxiv - Synthetic Biology 2024Quote: ... condition or GFP (7.7 mg mL-1 for experiments in Figs. 3a bottom and 3b bottom, 4b-c, Supplementary Figs. 8, 12; purchased from Chromotek, Germany) for NLS(- ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were blocked for 30 min in 1x Roti-block solution (Carl Roth GmbH, Karlsruhe) before applying rabbit anti-GFP 1:1000 (ChromoTek GmbH, Martinsried) over night at 4 °C in the same solution ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 50 µL of sepharose beads for 1 h at 4°C with constant rotation and subjected to immunoprecipitation with 25 µL GFP Trap agarose beads (Chromotek, gta-20) during 1 h at 4°C with constant rotation ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed and treated for immunofluorescence with α-SINV C antibody at 1:200 dilution and GFP-Booster_Atto488 (ChromoTek GmbH, #gba488-100) at 1:500 dilution following the procedure described in (Garcia-Moreno et al. ...
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bioRxiv - Cell Biology 2024Quote: ... thawed 90-nm-thick cryosections were incubated for 30 min at RT with a rabbit anti-GFP antibody (Chromotek, PABG1, 1:200 dilution) followed by protein A conjugated to 10 nm gold particles for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... The fixed cells were incubated for 1 hour in blocking solution (3% BSA in PBS) followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse mAb α-mNeonGreen (Chromotek #32f6-100), 1:500 ...