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Citations for ChromoTek :
1 - 50 of 491 citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and a red fluorescent protein tag (tdTomato; ChromoTek, 5f8-100, 1:500) expressed by the rabies virus ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Plant Biology 2023Quote: ... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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bioRxiv - Cell Biology 2023Quote: ... Halo-tag (mouse, 28a8 ChromoTek, 1:200), Alexa Fluor 647-direct labeled CETN1 (rabbit ...
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bioRxiv - Biophysics 2023Quote: ... Purified recombinant Enhanced Green Fluorescent Protein (EGFP) was purchased from Chromotek. Bafilomycin A1 was purchased from InvivoGen ...
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bioRxiv - Molecular Biology 2020Quote: ... rat monoclonal to myc-tag (Chromotek #9e1, 1:500), mouse monoclonal to Alpha Adaptin (Thermo #MA1-064 ...
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bioRxiv - Cell Biology 2024Quote: ... Detection of Venus-tagged proteins was based on anti-GFP tag Polyclonal IgG antibody (Chromotek) (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Overexpressed protein was immunoprecipitated from the cell lysate using tag specific nano-trap (SPOT-Trap-MA, Chromotek) and analysed by WB.
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bioRxiv - Neuroscience 2024Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Cell Biology 2023Quote: ... Spot tag (ChromoTek, Planegg-Martinsried, Germany), TOMM22 (Merck ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Biophysics 2022Quote: ... SNAP/CLIP-tag monoclonal antibody (6F9, Chromotek), single-domain antibody (nanobody ...
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bioRxiv - Immunology 2024Quote: ... Vimentin was labeled using Vimentin-Label Atto488 (1:200, ChromoTek, #vba448, Rosemont, IL) overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... The membrane was air-dried for 1 h and immunoblotted with a primary antibody against mNeonGreen tag (Chromotek) at room temperature for 1 h followed by a secondary antibody (680nm anti-mouse ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... PARP1-Chromobody (RFP-tag) plasmid was obtained from Chromotek (#xcr). All constructs were amplified with a Plasmid Midi Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant myc and myc-BDNF were from Chromotek and Cusabio ...
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bioRxiv - Developmental Biology 2023Quote: ... The CUT and TAG experiment was done for two biological replicates using antibodies anti-GFP (Chromotek, PABG1-100, 1:50), anti-YFP (Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... rat monoclonal anti-red fluorescent protein (RFP) (Chromotek, 1:1000) and goat anti-mCherry (1:1000) ...
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bioRxiv - Neuroscience 2022Quote: ... red fluorescent protein (RFP; rat, Chromotek: 5F8, RRID: AB_2336064; 1:2000) FoxP2 (mouse ...
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Co-release of GABA and ACh from medial olivocochlear neurons fine tunes cochlear efferent inhibitionbioRxiv - Physiology 2024Quote: ... (2) rat anti-Red Fluorescent Protein (RFP) (1:500; 5F8, Chromotek) to label tdTomato positive cells ...
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bioRxiv - Cell Biology 2024Quote: ... and the SPOT tag was detected using SPOT-Label Alexa Fluor 568 (ChromoTek #ebAF568). Both VHH nanobodies were used at a 1:1,000 dilution and incubated for 2 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The SPOT tag was detected using the SPOT-Label Alexa Fluor 568 (ChromoTek, Planegg-Martinsried, Germany) at a dilution of 1:1,000 ...
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bioRxiv - Genetics 2022Quote: ... 1 mg of protein extracts was incubated with agarose GFP traps (Chromotek, #GTA-20) pre-equilibrated with Buffer D (20mM HEPES pH 7.9 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 ml of protein lysate was incubated with 20 µl GFP-trap agarose (Chromotek) beads that were previously equilibrated with co-IP extraction buffer according to manufacturer’s directions at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were stained with rat anti-red fluorescent protein (RFP) (1:800, mAB 5F8, Chromotek) and then IRDye® 800CW goat anti-rat secondary antibody (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated for 1 h with 25 μl GFP trap (ChromoTek; Planegg-Martinsried, Germany) on a rotating wheel at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mg total proteins were incubated with 15 µl pre-equilibrated magnetic GFP-TRAP beads (Chromotek) for 2 h on a rotator at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Plant Biology 2023Quote: ... GFP tagged proteins were detected using an anti-GFP antibody (1:4000; mAb rat 3H9; Chromotek) and a secondary anti-rat antibody (1:15000 ...
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bioRxiv - Cell Biology 2024Quote: ... Xbp1s-GFP protein levels were analyzed by immunoblot with rat anti-GFP (1:1000, 3H9, Chromotek) and tubulin was used as loading control ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg of total protein was incubated with 25 μl of GFP-Trap® Magnetic Beads (ChromoTek) in 1 ml of extraction buffer at 4 °C for 2 h in a rotating wheel ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mL of protein lysate was incubated with 30 µl of HA-trap agarose (Chromotek, Illinois, USA) equilibrated beads at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... FITC MEF and micromolar concentration values were obtained by calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250).
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... benthamiana leaves were extracted at 1 mg ml−1 in extraction buffer as described14 and immuno-adsorbed by means of their GFP-tags on GFP-Trap agarose beads (ChromoTek). Immunoblots were developed either directly with Streptavidin-alkaline phosphatase conjugate (Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... Small cuts from the infiltrated regions were incubated for one hour in anti-Spot-Tag VHH/Nanobodies conjugated to the organic fluorophore ATTO488 (Chromotek), diluted 1:1000 in 10 mM Tris-HCl solution pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were then subjected western blotting probed by mouse anti-RFP (6g6, Chromotek, IL, USA) and mouse anti-α-tubulin antibodies (TAT-1 ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mL of the protein supernatant was incubated with 10 μL of magnetic GFP-Trap beads (Chromotek, Cat. No. gtma) or magnetic Myc-Trap beads (Chromotek ...
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bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
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bioRxiv - Microbiology 2022Quote: GFP-tagged target proteins and interacting proteins were affinity purified using GFP-trap agarose beads (Chromotek). Proteins were processed ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined and an equal amount of protein was mixed with TurboGFP-Trap Magnetic Agarose (Chromotek) for 1 hour at RT with agitation ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were detected using α-HA (Chromotek) and α-GFP antibodies (Santa Cruz Biotechnology ...