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Citations for ChromoTek :
1 - 50 of 1646 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Lysates from 21-GFP cells grown on two 150 mm dishes and infected with HSV-2 strain 186 at an MOI of 3 or uninfected were prepared at 18 hpi and immobilized on GFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies used: GFP Monoclonal antibody (3H9, Chromotek) 1:1000-2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with GFP-Trap magnetic beads (Chromotek) for 1.5 hours at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... or GFP-Trap magnetic agarose affinity beads (Chromotek, gtma-20) overnight at 4°C in a mini-rotator ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on 8% SDS-PAGE gels and detected by western blot using monoclonal mouse anti-HA (MMS-101R; Covance) or monoclonal mouse anti-RFP antibodies (6G6; ChromoTek) prior to secondary antibody treatment with polyclonal goat anti-mouse conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated with 25 μl of anti-HA magnetic beads (Pierce) or with 25 μl of GFP-Trap Magnetic Agarose (ChromoTek) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µl anti-GFP magnetic agarose beads (Chromotek) per sample were equilibrated by washing 3x with 500 µl lysis buffer ...
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bioRxiv - Plant Biology 2024Quote: ... VirE2-Venus and Venus cell lysates were incubated with GFP-Trap magnetic agarose beads (Chromotek, USA) at 4°C for 2 hr with gentle shaking ...
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bioRxiv - Plant Biology 2024Quote: ... GFP-tagged proteins were detected by α-GFP antibodies (ChromoTek).
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bioRxiv - Biochemistry 2024Quote: ... 1 mg of total protein was incubated with 25 μl of GFP-Trap® Magnetic Beads (ChromoTek) in 1 ml of extraction buffer at 4 °C for 2 h in a rotating wheel ...
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bioRxiv - Biochemistry 2024Quote: ... 20µl of GFP-Trap agarose (Proteintech (Chromotek), was applied to the supernatant and incubated overnight on a rotating platform at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Extracted proteins were then incubated with equilibrated GFP-trap beads (Chromotek) at 4°C for 2 h under gentle agitation ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then transferred to new collection tubes and incubated with to 30 µl of prewashed anti-myc or -V5 beads (Chromotek) and equilibrated to a final volume of 1000 µl by adding lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... GFP tagged AMPKγ3 was then immunoprecipitated by incubating 400 µg of protein with 1 µg of anti-GFP antibody (Chromotek, cat no. 3H9) and 15 µl of protein G sepharose beads (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was incubated in RFP-Trap magnetic beads (Chromotek) for 1 hour at 4°C on a rotator ...
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bioRxiv - Cell Biology 2024Quote: ... rat anti-HA (7C9) (Chromotek, 7c9-100 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation with MycTrap beads (Chromotek, Germany) for 1h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-trap agarose beads (ChromoTek, gta-10) were equilibrated in ice-cold wash/dilution buffer (20 mM Tris-HCl pH 7.7 ...
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bioRxiv - Cell Biology 2024Quote: ... thawed 90-nm-thick cryosections were incubated for 30 min at RT with a rabbit anti-GFP antibody (Chromotek, PABG1, 1:200 dilution) followed by protein A conjugated to 10 nm gold particles for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 µL of slurry of the GFP trap (Chromotek, #gta) were added to the lysate and rotated overnight at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mg nuclear or 3 mg cytoplasmic extract per pulldown was incubated in triplicates with 15 μl of a 50% slurry of GFP-trap beads (ChromoTek, gta-20) or control agarose beads (ChromoTek ...
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bioRxiv - Genetics 2024Quote: ... The GFP-Trap agarose kit (Chromotek) was used for immunoprecipitation according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-Trap agarose beads (ChromoTek) equilibrated in cold lysis buffer were incubated with the post-nuclear supernatant fraction for 3-5 h at 4°C with rotation ...
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bioRxiv - Plant Biology 2024Quote: ... GFP-Trap agarose beads (ChromoTek) were used and incubated with the crude extract for 3–4 hr at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... 40 mL of α-GFP beads (Chromotek) were added to the lysate for protein binding overnight at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... incubated with primary antibody α-RFP (mCherry) (Chromotek, 6G6, 1:2000) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 µl beads (GFP-Trap Agarose beads, Chromotek, Germany) were washed once with ice-cold 20 mM Tris pH 7.5 with 100mM NaCl (2,500 g ...
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bioRxiv - Plant Biology 2024Quote: ... between EIN2-C and HAF2 following study previous briefly: Total protein extracts from plants mentioned in the paper were immunoprecipitated by DYKDDDDK Fab-Trap™ agarose beads (ChromoTek) for two hours at 4 °C with gentle rotating and then beads were washed five times with ice-cold 1XPBS buffer ...
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bioRxiv - Plant Biology 2024Quote: ... GFP-trap beads (GFPTrap-A kit, Chromotek) were equilibrated with dilution buffer (same as the extraction buffer except for the omission of Triton X-100) ...
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bioRxiv - Neuroscience 2024Quote: ... labeled using primary antibody (mouse anti-mNEONGreen, 1:1000, ChromoTek) and secondary antibody (goat anti-mouse conjugated with Alexa Fluor 555 ...
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bioRxiv - Neuroscience 2024Quote: ... incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rat anti-RFP, 1:1000, 5f8, Chromotek; mouse anti-5mC ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-RFP (1:1000 Chromotek, 5F8) and rabbit anti-RFP (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... Lysate was incubated with anti-mNeonGreen nanobody-Trap Agarose beads (ChromoTek) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 20uL (ChromoTek) GFP beads were added and samples were incubated overnight at 4°C with gently agitation ...
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).
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bioRxiv - Plant Biology 2024Quote: ... Immunoprecipitation of the samples was performed following the GFP-Trap_MA protocol (Chromotek) using 6 µL of beads per sample and equilibrating the samples with 1.5 volumes of GFP-Trap_MA wash buffer ...
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S: ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-RFP (1:1,000; Chromotek #5f8) to reveal AAV-sgRNA-DIO-dTomato reporters ...
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bioRxiv - Cell Biology 2024Quote: ... and then 10 μL of GFP-Trap magnetic beads (Chromotek) was added to the supernatants an incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The remaining supernatant was incubated with 25 μl of GFP-nanotrap (gta-20, Chromotek) beads for 1h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated with GFP-Trap_A beads (ChromoTek, Thermo Fisher Scientific) on a rotator for 2 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 mL of the resulting supernatant (containing 2 – 4 mg of proteins) were supplemented with 15 μL of RFP-Trap magnetic particles (ChromoTek), and incubated for 2.5 h at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-Trap agarose beads (ChromoTek, cat # GT10) were first washed with IP buffer (10 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... 25 µL primary anti-RFP antibody (ChromoTek, 5f8) diluted 1:500 in YSS was added to each 250 µL pre-cleared lysate and incubated overnight at 4°C with rocking ...
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bioRxiv - Cell Biology 2024Quote: ... 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek) and incubated for 2 hours at 4°C with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: Samples for STED microscopy were prepared by incubating yeGFP-tagged Cdc3 cells (wild-type CDC11 or mutants) with a 1/250 dilution of an anti-GFP nanobody Atto 647N (ChromoTek), as previously detailed in (52) ...
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bioRxiv - Cell Biology 2024Quote: ... 1% NP-40) with protease and phosphatase inhibitors were incubated with GFP-Trap Agarose beads (Chromotek) in a rotation wheel for 6 h at 4°C ...