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Citations for ChromoTek :
401 - 450 of 1610 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and Nano-secondary AF647 (Chromotek) prepared in PBS with 10 % BSA and 0.1 % Triton X-100 for 1 hour at room temperature ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: For Figure 1E and 2C wild type or mutant versions of Faa1-EGFP were recruited to GFP-Trap_A beads beads (Chromotek). Assays were performed under equilibrium conditions with 1 mM of the prey liposomes in buffer (50 mM HEPES/Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 500 ug lysate was then added to GFP-Trap® Agarose resin (ChromoTek) and samples were incubated rotating at 4°C for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... Primary anti-V5 (ChromoTek; Catalog number: v5ab) was used in a dilution 1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was applied to GFP-Trap Agarose beads (Chromotek) equilibrated in the same buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein extracts were incubated with goat anti-GFP Trap agarose (gta-20, Chromotek) or anti-c-Myc Agarose (20168 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was incubated with 50 µl HNG-washed HaloTrap-agarose beads (Chromotek) with end-over-end rotation for 15 minutes hours to capture specific binding proteins ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged for 20 min (full speed, 4°C) and the supernatant was incubated with mEGFP-Trap Agarose beads (ChromoTek) for 1 hour according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a monoclonal anti-mNeonGreen antibody (Chromotek, Germany ...
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bioRxiv - Cell Biology 2023Quote: ... mNeonGreen (Cat# 32f6; 1:1,000 dilution; ChromoTek). Immunoblots were developed by binding HRP-conjugated anti-rabbit IgG (ab6721;1:5,000 ...
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bioRxiv - Immunology 2023Quote: ... 2 mg of cell lysates were incubated with 25 ml of GFP-TRAP beads (Chromotek, GTA-20) for 1 hr at 4°C on rotation ...
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bioRxiv - Biochemistry 2023Quote: ... IGF2BP1 was immunoprecipitated from HEK293T cells by GFP-trap magnetic beads (ChromoTek). The cells were lysed using cold lysis buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... RFP-Trap agarose beads (Chromotek, rta) were used following a similar protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were centrifuged at 21,000 g at 4 °C for 10 min and then incubated for 10 min at 4 °C with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek) rotating ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... Atg9-EGFP PLs were immobilized on GFP-TRAP_A beads (Chromotek). The assay was performed under equilibrium conditions with a prey concentration of 1 µM Faa1-mCherry ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was incubated at 4°C for 1 h with GFP-Trap Agarose beads (Chromotek) and washed 4 times ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µl of RFP-trap beads (Chromotek) were equilibrated by washing them three times with wash buffer (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... blotted to nitrocellulose membranes and incubated with antibodies specific for GFP (Chromotek, 3h9), RFP (Chromotek ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was loaded onto 70 μL of GFP-Trap beads (Chromotek gtma-20) which were prewashed once with RIPA wash buffer (as above ...
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bioRxiv - Molecular Biology 2023Quote: ... transferred to Nitrocellulose membranes and analysed with anti-RFP (Chromotek) and anti-Flag-HRP conjugated (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Dynabeads were replaced with 25 µL/sample of GFP-Trap beads (ChromoTek), which are covalently bound to a highly efficient alpaca-raised nanobody against the tag ...
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bioRxiv - Molecular Biology 2023Quote: ... rat anti-GFP (Chromotek, 3H9) (diluted 1:1000) ...
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bioRxiv - Microbiology 2023Quote: ... After removing cell debris by centrifugation 10ul GFP-trap Sepharose (Chromotek) slurry was added to the lysate and incubation was carried out for 1.5 hours on a rotating shaker at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was pre-cleared with Sepharose beads and subjected to immunoprecipitation with GFP-Trap beads (Chromotek). After extensive washing with RIP washing buffer ...
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bioRxiv - Plant Biology 2023Quote: ... protein extracts were incubated with GFP-Trap Agarose (Chromotek, Germany) at 4°C for 1 h with gentle rotation ...
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bioRxiv - Cell Biology 2023Quote: ... 80 µL GFP-Trap slurry (ChromoTek) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were measured by Bradford method and lysates were then incubated with rotation in pre-washed GFP-TRAP beads (Chromotek) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 80 µL of RFP-Trap slurry (ChromoTek) was used ...
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bioRxiv - Biochemistry 2023Quote: ... or GFP-trap magnetic agarose (Chromotek, to precipitate GFP-tagged Cx43) were rocked with 200 µL of clarified cell lysate for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Developmental Biology 2023Quote: ... 25μl of GFP-Trap beads (GFP-Trap Dynabeads, Chromotek #gtd-20) per lysate were used for GFP pulldown from experiment Tg(buc:Buc-GFP) ...
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bioRxiv - Cell Biology 2023Quote: ... an mNeonGreen-Trap Agarose Kit (ntak-20; Chromotek) to pull down Tld1-mNeonGreen (mNG ...
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bioRxiv - Developmental Biology 2023Quote: ... and 25μl of control no-GFP nanobody beads (Chromotek), were used for IP control on Tg(buc:Buc-GFP ...
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bioRxiv - Neuroscience 2023Quote: ... a GFP booster stain (Chromotek Alexa Fluor 488) at 1:500 and DAPI stain (Thermofisher D1306 ...
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bioRxiv - Neuroscience 2023Quote: ... anti-RFP (rat monoclonal, 1:1000, Chromotek #5f8 used to stain for virally-expressed tdTomato only in the dual immuno-in situ while for all other experiments endogenous tdTomato fluorescence was visualized) ...
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bioRxiv - Plant Biology 2023Quote: ... and the remaining supernatant (∼2.7 ml) was incubated with 30 µL of GFP-Trap magnetic agarose beads (GFP-TrapMA; Chromotek) for 2 h at 4°C by gentle vertical rotation ...
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bioRxiv - Neuroscience 2023Quote: ... 250 μL of pre-rinsed anti-GFP nanobody magnetic beads slurry (Chromotek) in 10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: GFP-trap agarose (Chromotek) or anti-Flag M2 affinity gel (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... 300 mg tissue from whole seedlings was used with 30 µl of GFP magnetic trap beads (Chromotek). The plants were ground to powder in liquid nitrogen and lysed in 2.5[ml cold nuclei extraction buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated with magnetic agarose GFP-Trap beads (Chromotek) for 2-3 h at 4°C with end-to-end rotation ...
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bioRxiv - Cell Biology 2023Quote: ... MYC-tagged protein was purified from transduced cells (see above) with Myc-trap agarose (Chromotek), a nanobody based system ...
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bioRxiv - Plant Biology 2023Quote: ... Immunogold labelling of CASP1-GFP was carried out according to [45] using a 1:5 dilution of rat monoclonal anti-GFP 3H9 (Chromotek) and a 1:20 dilution of goat anti-rat IgG conjugated to 10 nm colloidal gold particles (British Biocell International) ...
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bioRxiv - Biophysics 2023Quote: ... The membrane was air-dried for 1 h and immunoblotted with a primary antibody against mNeonGreen tag (Chromotek) at room temperature for 1 h followed by a secondary antibody (680nm anti-mouse ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Trap beads (Chromotek) were equilibrated and washed 3 times in 500 µL of ice-cold RIPA buffer and were spun down at 2500 × g for 2 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM Tris pH 7.5 and 5 µl of equilibrated GFP-Trap beads (Chromotek) and incubated overnight at 4°C with constant mixing ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitation was carried out using the GFP-Trap Agarose KIT (ChromoTek), following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Cell Biology 2023Quote: ... 80 µl GFP trap Dynabeads (Chromotek) were washed with ice cold lysis buffer 3x and collected by spinning at 2000g for min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining supernatants were mixed with GFP-Trap magnetic beads (8 µL of slurry per sample; Chromotek, gtma-20) prewashed with IP Buffer [500 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... EGFP-NLRP3 was immunoprecipitated with GFP-Trap (Chromotek). The agarose beads were washed with IP washing buffer (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... 40 µl of GFP-TRAP beads (Chromotek) beads were added and samples were incubated for 30 minutes at 4°C ...